LOCUS       NM_007244                563 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens proline rich 4 (PRR4), transcript variant 2, mRNA.
ACCESSION   NM_007244
VERSION     NM_007244.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 563)
  AUTHORS   Perumal,N., Funke,S., Wolters,D., Pfeiffer,N. and Grus,F.H.
  TITLE     Characterization of human reflex tear proteome reveals high
            expression of lacrimal proline-rich protein 4 (PRR4)
  JOURNAL   Proteomics 15 (19), 3370-3381 (2015)
   PUBMED   26173177
  REMARK    GeneRIF: High expression of lacrimal proline-rich protein 4 (PRR4)
            was found in reflex tears.
REFERENCE   3  (bases 1 to 563)
  AUTHORS   Perumal,N., Funke,S., Pfeiffer,N. and Grus,F.H.
  TITLE     Characterization of lacrimal proline-rich protein 4 (PRR4) in human
            tear proteome
  JOURNAL   Proteomics 14 (13-14), 1698-1709 (2014)
   PUBMED   24782455
  REMARK    GeneRIF: A new PRR4 isoform designated as PRR4-N3 in addition to
            PRR4 (gi154448886) and pHL E1F1 (gi1050983) was identified in human
            tear proteome.
REFERENCE   4  (bases 1 to 563)
  AUTHORS   Pieragostino,D., Agnifili,L., Fasanella,V., D'Aguanno,S.,
            Mastropasqua,R., Di Ilio,C., Sacchetta,P., Urbani,A. and Del
            Boccio,P.
  TITLE     Shotgun proteomics reveals specific modulated protein patterns in
            tears of patients with primary open angle glaucoma naive to therapy
  JOURNAL   Mol Biosyst 9 (6), 1108-1116 (2013)
   PUBMED   23580065
REFERENCE   5  (bases 1 to 563)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   6  (bases 1 to 563)
  AUTHORS   Aluru,S.V., Agarwal,S., Srinivasan,B., Iyer,G.K., Rajappa,S.M.,
            Tatu,U., Padmanabhan,P., Subramanian,N. and Narayanasamy,A.
  TITLE     Lacrimal proline rich 4 (LPRR4) protein in the tear fluid is a
            potential biomarker of dry eye syndrome
  JOURNAL   PLoS One 7 (12), e51979 (2012)
   PUBMED   23272196
  REMARK    GeneRIF: Lacrimal proline rich 4 (LPRR4) protein in the tear fluid
            is a potential biomarker of dry eye syndrome.
REFERENCE   7  (bases 1 to 563)
  AUTHORS   Fung,K.Y., Morris,C., Sathe,S., Sack,R. and Duncan,M.W.
  TITLE     Characterization of the in vivo forms of lacrimal-specific
            proline-rich proteins in human tear fluid
  JOURNAL   Proteomics 4 (12), 3953-3959 (2004)
   PUBMED   15526346
REFERENCE   8  (bases 1 to 563)
  AUTHORS   Dickinson,D.P. and Thiesse,M.
  TITLE     A major human lacrimal gland mRNA encodes a new proline-rich
            protein family member
  JOURNAL   Invest Ophthalmol Vis Sci 36 (10), 2020-2031 (1995)
   PUBMED   7544782
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB229285.1 and AF530472.1.
            
            On Nov 23, 2018 this sequence version replaced NM_007244.2.
            
            Summary: This gene encodes a member of the proline-rich protein
            family that lacks a conserved repetitive domain. This protein may
            play a role in protective functions in the eye. Alternative
            splicing result in multiple transcript variants. Read-through
            transcription also exists between this gene and the upstream PRH1
            (proline-rich protein HaeIII subfamily 1) gene. [provided by
            RefSeq, Feb 2011].
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP343443.1, BP343614.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA2150585
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228811.8/ ENSP00000228811.4
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DB229285.1         6-22
            18-563              AF530472.1         1-546
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.2"
     gene            1..563
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /note="proline rich 4"
                     /db_xref="GeneID:11272"
                     /db_xref="HGNC:HGNC:18020"
                     /db_xref="MIM:605359"
     exon            1..102
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..443
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; proline-rich polypeptide 4; proline rich 4
                     (lacrimal); lacrimal proline-rich protein; nasopharyngeal
                     carcinoma-associated proline-rich protein 4"
                     /codon_start=1
                     /product="proline-rich protein 4 isoform 2 precursor"
                     /protein_id="NP_009175.2"
                     /db_xref="CCDS:CCDS41756.1"
                     /db_xref="GeneID:11272"
                     /db_xref="HGNC:HGNC:18020"
                     /db_xref="MIM:605359"
                     /translation="MLLVLLSVVLLALSSAQSTDNDVNYEDFTFTIPDVEDSSQRPDQ
                     GPQRPPPEGLLPRPPGDSGNQDDGPQQRPPKPGGHHRHPPPPPFQNQQRPPRRGHRQL
                     SLPRFPSVSLQEASSFFQRDRPARHPQEQPLW"
     exon            103..138
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /inference="alignment:Splign:2.1.0"
     exon            139..461
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /inference="alignment:Splign:2.1.0"
     exon            462..563
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      541..546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /note="hexamer: AATAAA"
     polyA_site      563
                     /gene="PRR4"
                     /gene_synonym="LPRP; PROL4"
                     /note="major polyA site"
ORIGIN      
        1 aacgcagagt tgggagcaac tccagagcct ccttcaagat gctgctggtc ctgctctcag
       61 tggtccttct ggctctgagc tcagctcaga gcacagataa tgatgtgaac tatgaagact
      121 ttactttcac cataccagat gtagaggact caagtcagag accagatcag ggaccccaga
      181 gacctcctcc tgaaggactc ctacctagac cccctggtga tagtggtaac caagatgatg
      241 gtcctcagca gagaccacca aaaccaggag gccatcaccg ccatcctccc ccacctcctt
      301 ttcaaaatca gcaacgacca ccccgacgag gacaccgtca actctctcta ccccgatttc
      361 cttctgtcag cctgcaggaa gcatcatcat tcttccagag ggacagacca gcaagacatc
      421 cccaggagca accactctgg taatctagaa ttcagtggca gaaaataaat aagaagataa
      481 cttccttcag aaagccatga cattgaaata atgtggtcat aactctttct tcagtatacc
      541 aataaaatat taatagcatg caa
//
