LOCUS       NM_002765               2469 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens phosphoribosyl pyrophosphate synthetase 2 (PRPS2),
            transcript variant 2, mRNA.
ACCESSION   NM_002765
VERSION     NM_002765.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Luo,Y., Yuan,J., Huang,J., Yang,T., Zhou,J., Tang,J., Liu,M.,
            Chen,J., Chen,C., Huang,W. and Zhang,H.
  TITLE     Role of PRPS2 as a prognostic and therapeutic target in
            osteosarcoma
  JOURNAL   J Clin Pathol 74 (5), 321-326 (2021)
   PUBMED   33589531
  REMARK    GeneRIF: Role of PRPS2 as a prognostic and therapeutic target in
            osteosarcoma.
            Erratum:[J Clin Pathol. 2021 Aug;74(8):e12. doi:
            10.1136/jclinpath-2020-206505corr1.. PMID: 34282027]
REFERENCE   3  (bases 1 to 2469)
  AUTHORS   Yang,Y., Song,L., Huang,X., Feng,Y., Zhang,Y., Liu,Y., Li,S.,
            Zhan,Z., Zheng,L., Feng,H. and Li,Y.
  TITLE     PRPS1-mediated purine biosynthesis is critical for pluripotent stem
            cell survival and stemness
  JOURNAL   Aging (Albany NY) 13 (3), 4063-4078 (2021)
   PUBMED   33493137
  REMARK    GeneRIF: PRPS1-mediated purine biosynthesis is critical for
            pluripotent stem cell survival and stemness.
REFERENCE   4  (bases 1 to 2469)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2469)
  AUTHORS   Lei,B., Zhang,S.B., Xie,L.X. and Mao,X.M.
  TITLE     [Expression of phosphoribosyl pyrophosphate synthase 2 in human
            testis tissue]
  JOURNAL   Zhonghua Nan Ke Xue 26 (2), 128-133 (2020)
   PUBMED   33346415
  REMARK    GeneRIF: [Expression of phosphoribosyl pyrophosphate synthase 2 in
            human testis tissue].
REFERENCE   6  (bases 1 to 2469)
  AUTHORS   Dawson,S.J. and White,L.A.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J Infect 24 (3), 317-320 (1992)
   PUBMED   1602151
REFERENCE   7  (bases 1 to 2469)
  AUTHORS   Ishizuka,T., Iizasa,T., Taira,M., Ishijima,S., Sonoda,T.,
            Shimada,H., Nagatake,N. and Tatibana,M.
  TITLE     Promoter regions of the human X-linked housekeeping genes PRPS1 and
            PRPS2 encoding phosphoribosylpyrophosphate synthetase subunit I and
            II isoforms
  JOURNAL   Biochim Biophys Acta 1130 (2), 139-148 (1992)
   PUBMED   1314091
REFERENCE   8  (bases 1 to 2469)
  AUTHORS   Becker,M.A., Heidler,S.A., Bell,G.I., Seino,S., Le Beau,M.M.,
            Westbrook,C.A., Neuman,W., Shapiro,L.J., Mohandas,T.K.,
            Roessler,B.J. et al.
  TITLE     Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases
            1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes
  JOURNAL   Genomics 8 (3), 555-561 (1990)
   PUBMED   1962753
REFERENCE   9  (bases 1 to 2469)
  AUTHORS   Taira,M., Iizasa,T., Yamada,K., Shimada,H. and Tatibana,M.
  TITLE     Tissue-differential expression of two distinct genes for
            phosphoribosyl pyrophosphate synthetase and existence of the
            testis-specific transcript
  JOURNAL   Biochim Biophys Acta 1007 (2), 203-208 (1989)
   PUBMED   2537655
REFERENCE   10 (bases 1 to 2469)
  AUTHORS   Taira,M., Kudoh,J., Minoshima,S., Iizasa,T., Shimada,H.,
            Shimizu,Y., Tatibana,M. and Shimizu,N.
  TITLE     Localization of human phosphoribosylpyrophosphate synthetase
            subunit I and II genes (PRPS1 and PRPS2) to different regions of
            the X chromosome and assignment of two PRPS1-related genes to
            autosomes
  JOURNAL   Somat Cell Mol Genet 15 (1), 29-37 (1989)
   PUBMED   2536962
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA478574.1, BC110875.1 and
            BU675363.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_002765.4.
            
            Summary: This gene encodes a phosphoribosyl pyrophosphate
            synthetase that plays a central role in the synthesis of purines
            and pyrimidines. The encoded protein catalyzes the synthesis of
            5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate.
            Alternate splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2010].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. This
            results in a shorter protein (isoform 2), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040483.1, Y00971.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380668.10/ ENSP00000370043.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA478574.1         58-66
            10-2218             BC110875.1         1-2209
            2219-2469           BU675363.1         24-274              c
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2"
     gene            1..2469
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /note="phosphoribosyl pyrophosphate synthetase 2"
                     /db_xref="GeneID:5634"
                     /db_xref="HGNC:HGNC:9465"
                     /db_xref="MIM:311860"
     exon            1..208
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1043
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /EC_number="2.7.6.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ribose-phosphate diphosphokinase 2; ribose-phosphate
                     pyrophosphokinase 2; PPRibP synthetase; PRS-II;
                     phosphoribosyl pyrophosphate synthase II; testicular
                     secretory protein Li 41"
                     /codon_start=1
                     /product="ribose-phosphate pyrophosphokinase 2 isoform 2"
                     /protein_id="NP_002756.1"
                     /db_xref="CCDS:CCDS14150.1"
                     /db_xref="GeneID:5634"
                     /db_xref="HGNC:HGNC:9465"
                     /db_xref="MIM:311860"
                     /translation="MPNIVLFSGSSHQDLSQRVADRLGLELGKVVTKKFSNQETSVEI
                     GESVRGEDVYIIQSGCGEINDNLMELLIMINACKIASSSRVTAVIPCFPYARQDKKDK
                     SRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLQWIRENIA
                     EWKNCIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVDRMVLVGDVKDRVAIL
                     VDDMADTCGTICHAADKLLSAGATKVYAILTHGIFSGPAISRINNAAFEAVVVTNTIP
                     QEDKMKHCTKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL"
     exon            209..392
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     exon            393..491
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     exon            492..616
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     exon            617..790
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     exon            791..950
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     exon            951..2469
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2445..2450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /note="hexamer: AATAAA"
     polyA_site      2469
                     /gene="PRPS2"
                     /gene_synonym="PRSII"
                     /note="major polyA site"
ORIGIN      
        1 gcagcagcag cctcccgcgt cgctgtcgct gttgcctccg ccacctcctc cgccgccgcg
       61 cgcccctcgg agttccgcgc cccaccatgc ccaacatcgt gctgttcagc ggcagctcgc
      121 atcaggacct gtcccagcgc gtggccgacc gcctgggcct ggagctgggc aaggtggtca
      181 cgaagaagtt cagcaaccag gagaccagcg tggagattgg tgaaagcgtg agaggggaag
      241 atgtctacat catccagagc ggctgcgggg aaattaacga caacctgatg gaactcctca
      301 tcatgatcaa tgcctgcaag attgcgtcat catccagagt aactgccgtg atcccgtgtt
      361 tcccatacgc ccgacaagat aaaaaggaca agagtcgtgc cccaatttct gcaaaacttg
      421 tggccaatat gctgtcggtg gctggggcgg atcacatcat caccatggac ctgcatgctt
      481 ctcagataca gggattcttt gatattcctg tggataattt gtatgcggag cccgcagtcc
      541 tgcagtggat tcgggaaaac attgccgagt ggaagaactg tatcattgtt tcacctgacg
      601 cagggggagc caaaagggtt acatcaattg cagacaggtt gaatgtggaa tttgctttga
      661 tccacaaaga gaggaagaag gcgaatgaag tggaccggat ggtcctggtg ggcgacgtga
      721 aggaccgtgt ggccatcctc gtggatgaca tggctgacac ttgcggcacc atctgccatg
      781 ctgcggacaa gctgctgtca gctggagcca ccaaagtgta tgctatcctt acccatggga
      841 tcttctctgg accagctatt tccagaataa ataatgccgc ctttgaggct gttgtcgtca
      901 caaacacaat tccgcaagag gacaaaatga aacactgcac caagattcag gtcattgaca
      961 tttccatgat cttggccgaa gcaatccgaa ggacacacaa tggggaatcc gtgtcctacc
     1021 tgttcagcca tgtcccgcta taaatccaga atgggaagtg tccagcaagc ctactctgac
     1081 ttctgacttg tttttgtttt ctggattttt agctgtaggt attcagcaat gataggttaa
     1141 tcactggcaa aagcatcaga tctttgtata tgctaagatt tattgtttcc ccttctaaag
     1201 ctcaagatca tttctttcca gtttttgggg aaatggtggt ggttatttgg tctttaagtg
     1261 aactgtctta aatgagaaac gtttttgtca ttttgacttt taacaggtac aggtgatctc
     1321 ttcctttgtt ctttcagtac tttgaggcga caactttcaa gtatataatt tcattgtgga
     1381 agtcatagtt tatatatttc gaggttgcca aaggtgactt cagattaaag ccttctgtgt
     1441 aaatatatac tgataatgcc tatggacatt tgggtaaaac cctgtataga attaattatc
     1501 cttttacttt ggagtgaacc ttggaaaatt tataattata ataccatgga ttttgaattt
     1561 tccttttttt tttttttttg gataactcag tttcagataa accatcttgg ttactgtgct
     1621 taatttggac caaattttat ttagcttaat atggacactg acacattttg gggggtatac
     1681 attagacata tcagagcagt gtatttctgg atcatttttt aaatgacctc ttctaaaaca
     1741 taactgtcac ttacctgaaa tgctgcatcc taaaattcca aaattatatt gagcaatcgc
     1801 caaggcctaa agccaactga cttaaaggta atcatttcag ctaagattaa atttaaagcc
     1861 taagaatgta tagagctagt tttaaaataa tgatctcaga tttttaaaaa ggatatagga
     1921 acctgcattg tcattctctg aattaagaac tgatggtttc tatcattatt tagccccacc
     1981 tttgtatttt aaaatccttc agaatacatt tatgaaccaa tgcgactgga cttagccaca
     2041 cacaatggaa attcagacct tgactatttg gtgtttccag ttcacaaagg tgatgaagac
     2101 tgtcttggga gcagcttaat cccaaaattt gtacatttct tgctgctcct ggcgtggaaa
     2161 cttaagtgag accaccaaat acattggtcc tgtccaattc tactgaatgg gggtggacct
     2221 ggcatttatc tggccaaaaa caggagccag agaaatatga atataccaaa gttgtttgtt
     2281 tagcctccaa cttaaattac attagtcaac ttatagatac tcatatgatc acttttcttt
     2341 ttagatacta catcaactag attcaggagt atatcatttg cagtgcttgt attggtttaa
     2401 aatgtaagat tttaagatcc tctaacactg tactaaaaca tttcaataaa atcattctga
     2461 ctgcgttca
//
