LOCUS       NM_001244926            2897 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens pre-mRNA splicing tri-snRNP complex factor PRPF4
            (PRPF4), transcript variant 2, mRNA.
ACCESSION   NM_001244926
VERSION     NM_001244926.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Park,S., Han,S.H., Kim,H.G., Jeong,J., Choi,M., Kim,H.Y., Kim,M.G.,
            Park,J.K., Han,J.E., Cho,G.J., Kim,M.O., Ryoo,Z.Y. and Choi,S.K.
  TITLE     PRPF4 is a novel therapeutic target for the treatment of breast
            cancer by influencing growth, migration, invasion, and apoptosis of
            breast cancer cells via p38 MAPK signaling pathway
  JOURNAL   Mol Cell Probes 47, 101440 (2019)
   PUBMED   31445970
  REMARK    GeneRIF: Results confirmed that the PRPF4 gene was overexpressed in
            various breast cancer cell lines. Its knockdown slows down breast
            cancer progression via suppression of p38 MAPK phosphorylation. In
            conclusion, the PRPF4 gene plays an important role in the growth of
            breast cancer cells.
REFERENCE   3  (bases 1 to 2897)
  AUTHORS   Charenton,C., Wilkinson,M.E. and Nagai,K.
  TITLE     Mechanism of 5' splice site transfer for human spliceosome
            activation
  JOURNAL   Science 364 (6438), 362-367 (2019)
   PUBMED   30975767
REFERENCE   4  (bases 1 to 2897)
  AUTHORS   Islam,S.U., Ahmed,M.B., Lee,S.J., Shehzad,A., Sonn,J.K., Kwon,O.S.
            and Lee,Y.S.
  TITLE     PRP4 kinase induces actin rearrangement and epithelial-mesenchymal
            transition through modulation of the actin-binding protein cofilin
  JOURNAL   Exp Cell Res 369 (1), 158-165 (2018)
   PUBMED   29787735
  REMARK    GeneRIF: Possible molecular mechanism of PRP4-induced actin
            cytoskeleton remodeling and epithelial-mesenchymal transition,
            makes PRP4 an important target in colon cancer.
REFERENCE   5  (bases 1 to 2897)
  AUTHORS   Trepte,P., Kruse,S., Kostova,S., Hoffmann,S., Buntru,A.,
            Tempelmeier,A., Secker,C., Diez,L., Schulz,A., Klockmeier,K.,
            Zenkner,M., Golusik,S., Rau,K., Schnoegl,S., Garner,C.C. and
            Wanker,E.E.
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology
            for quantitative mapping of protein-protein interactions in
            mammalian cells
  JOURNAL   Mol Syst Biol 14 (7), e8071 (2018)
   PUBMED   29997244
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2897)
  AUTHORS   Heng,H.H., Wang,A. and Hu,J.
  TITLE     Mapping of the human HPRP3 and HPRP4 genes encoding
            U4/U6-associated splicing factors to chromosomes 1q21.1 and
            9q31-q33
  JOURNAL   Genomics 48 (2), 273-275 (1998)
   PUBMED   9521884
REFERENCE   7  (bases 1 to 2897)
  AUTHORS   Horowitz,D.S., Kobayashi,R. and Krainer,A.R.
  TITLE     A new cyclophilin and the human homologues of yeast Prp3 and Prp4
            form a complex associated with U4/U6 snRNPs
  JOURNAL   RNA 3 (12), 1374-1387 (1997)
   PUBMED   9404889
REFERENCE   8  (bases 1 to 2897)
  AUTHORS   Wang,A., Forman-Kay,J., Luo,Y., Luo,M., Chow,Y.H., Plumb,J.,
            Friesen,J.D., Tsui,L.C., Heng,H.H., Woolford,J.L. Jr. and Hu,J.
  TITLE     Identification and characterization of human genes encoding Hprp3p
            and Hprp4p, interacting components of the spliceosome
  JOURNAL   Hum Mol Genet 6 (12), 2117-2126 (1997)
   PUBMED   9328476
REFERENCE   9  (bases 1 to 2897)
  AUTHORS   Lauber,J., Plessel,G., Prehn,S., Will,C.L., Fabrizio,P.,
            Groning,K., Lane,W.S. and Luhrmann,R.
  TITLE     The human U4/U6 snRNP contains 60 and 90kD proteins that are
            structurally homologous to the yeast splicing factors Prp4p and
            Prp3p
  JOURNAL   RNA 3 (8), 926-941 (1997)
   PUBMED   9257651
  REMARK    Erratum:[RNA 1997 Oct;3(10):1204-6]
REFERENCE   10 (bases 1 to 2897)
  AUTHORS   Horowitz,D.S. and Krainer,A.R.
  TITLE     A human protein required for the second step of pre-mRNA splicing
            is functionally related to a yeast splicing factor
  JOURNAL   Genes Dev 11 (1), 139-151 (1997)
   PUBMED   9000057
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA815191.1, BC007424.2,
            BC001588.2, AW052006.1 and CA420073.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_001244926.1.
            
            Summary: The protein encoded by this gene is part of a heteromeric
            complex that binds U4, U5, and U6 small nuclear RNAs and is
            involved in pre-mRNA splicing. The encoded protein also is a
            mitotic checkpoint protein and a regulator of chemoresistance in
            human ovarian cancer. Several transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Apr 2016].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction at the 5' end of an exon compared to variant 1. The
            resulting isoform (2) has the same N- and C-termini but is 1 aa
            shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225056.1, SRR18074968.3570576.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374198.5/ ENSP00000363313.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DA815191.1         25-47
            24-2219             BC007424.2         1-2196
            2220-2768           BC001588.2         2187-2735
            2769-2880           AW052006.1         2-113               c
            2881-2897           CA420073.1         18-34               c
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q32"
     gene            1..2897
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /note="pre-mRNA splicing tri-snRNP complex factor PRPF4"
                     /db_xref="GeneID:9128"
                     /db_xref="HGNC:HGNC:17349"
                     /db_xref="MIM:607795"
     exon            1..113
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1652
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /note="isoform 2 is encoded by transcript variant 2;
                     PRP4/STK/WD splicing factor; U4/U6 small nuclear
                     ribonucleoprotein Prp4; PRP4 homolog; WD splicing factor
                     Prp4; U4/U6 snRNP 60 kDa protein; pre-mRNA processing
                     factor 4; PRP4 pre-mRNA processing factor 4 homolog"
                     /codon_start=1
                     /product="U4/U6 small nuclear ribonucleoprotein Prp4
                     isoform 2"
                     /protein_id="NP_001231855.1"
                     /db_xref="CCDS:CCDS59142.1"
                     /db_xref="GeneID:9128"
                     /db_xref="HGNC:HGNC:17349"
                     /db_xref="MIM:607795"
                     /translation="MASSRASSTATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAK
                     GESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVST
                     DDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEE
                     YQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQMQELHKSLR
                     SLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVG
                     AIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSG
                     RFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWD
                     LRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNL
                     VTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIAT
                     CSYDRTFKLWMAE"
     exon            114..291
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            292..478
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            479..566
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            567..646
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            647..740
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            741..835
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            836..894
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1018
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1108
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1231
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1339
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1458
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..2897
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2742..2747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /note="hexamer: ATTAAA"
     polyA_site      2776
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /note="major polyA site"
     polyA_site      2802
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
     polyA_site      2851
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
     regulatory      2855..2860
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
                     /note="hexamer: TATAAA"
     polyA_site      2897
                     /gene="PRPF4"
                     /gene_synonym="HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60"
ORIGIN      
        1 gcacttcccc tctgctgggc gcgcggtgga cggtctgaaa gggagtgttc gggtttcgct
       61 ggggcctcgc ggctccagag cccagcatgg cttcctcgcg agcctcttcc acggcaacca
      121 aaactaaagc acccgacgac ttagttgctc cggtcgtgaa gaaaccacac atctattatg
      181 gaagtttgga agagaaggag agggagcgtc tggccaaagg agagtctggg attttgggga
      241 aagacggact taaagcaggg atcgaagctg gaaatattaa tataacctct ggagaagtgt
      301 ttgaaattga agagcatatc agcgagcgac aggcagaagt attggctgag tttgagagaa
      361 ggaagcgagc ccggcagatc aatgtttcca cagatgactc agaggtcaaa gcttgcctta
      421 gagccttggg ggaacccatc acactttttg gagagggtcc tgctgaaaga agagaaaggt
      481 taagaaatat cctctcagtt gtcggtactg atgccttgaa aaagaccaaa aaggatgatg
      541 agaagtctaa aaagtccaaa gaagagtatc agcaaacctg gtatcatgaa ggaccaaata
      601 gcttgaaggt ggcaagacta tggattgcta attattcgtt gcccagggca atgaaacgct
      661 tggaagaggc ccgactccat aaggagattc ctgagacaac aaggacctcc cagatgcaag
      721 agctgcacaa gtctctccgg tctttgaata atttttgcag tcagattggg gatgatcggc
      781 ctatctccta ctgtcacttt agtcccaatt ccaagatgct ggccacagct tgttggagtg
      841 ggctttgcaa gctctggtct gttcctgatt gcaacctcct tcacactctt cgagggcata
      901 acacaaatgt aggagcaatt gtattccatc ccaaatccac tgtctccttg gacccaaaag
      961 atgtcaacct ggcctcttgt gcggctgatg gctctgtgaa gctttggagt ctcgacagtg
     1021 atgaaccagt ggcagatatt gaaggccata cagtgcgtgt ggcgcgggta atgtggcatc
     1081 cttcaggacg tttcctgggc accacctgct atgaccgttc atggcgctta tgggatttgg
     1141 aggctcaaga ggagatcctg catcaggaag gccatagcat gggtgtgtat gacattgcct
     1201 tccatcaaga tggctctttg gctggcactg ggggactgga tgcatttggt cgagtttggg
     1261 acctacgcac aggacgttgt atcatgttct tagaaggcca cctgaaagaa atctatggaa
     1321 taaatttctc ccccaatggc tatcacattg caaccggcag tggtgacaac acctgcaaag
     1381 tgtgggacct ccgacagcgg cgttgcgtct acaccatccc tgctcatcag aacttagtga
     1441 ctggtgtcaa gtttgagcct atccatggga acttcttgct tactggtgcc tatgataaca
     1501 cagccaagat ctggacgcac ccaggctggt ccccgctgaa gactctggct ggccacgaag
     1561 gcaaagtgat gggcctagat atttcttccg atgggcagct catagccact tgctcatatg
     1621 acaggacctt caagctgtgg atggctgaat agatgacaat gggaaaagga cttgaacctc
     1681 aagctctctc taaggagctg ttttcctcaa acgagaagaa ttgaagtgtt tagttctatc
     1741 atgttttctg ccaattacca tgcatagacc ctcagtagaa ttggatttcc atgtcagccc
     1801 ccactccagg aaggcagccc aatccctagg tgatggggaa cccctctcac ggttgaaaat
     1861 ttattacctt tttacgccct gccacgaact gtgtagacat tgtttttatt aatcttttgt
     1921 ttggccgggc gtggtggctc acgcctgtaa tcctagcact ttgggaggcc gaggtgggta
     1981 gatcgcttga gctcaggagt tcaagacgag cctgggcaac atggcaaatg ccgtctctgc
     2041 aaaaaaatac taaaattagc tggtcgcggt ggcttctgcc tgtgattccg gctacttggg
     2101 aggctgaggt gggagggatt gcttaagcct gggaggcaga ggttgcagtg agccgagatt
     2161 gcgccattgc actctagcct gtgtgacaga gcaagaccct gtctcaaaaa aaaaaaaaat
     2221 ttgttcgaat gccttatagc cttcctcaca gcacccagga ttgtgactga ctctgcattt
     2281 ttaattcttg aaacttggct ttccataaca tggtacatgc ttcaggacta catatgaccc
     2341 agagagcaag gtggctgaac tatagtctgg aagccctcag gtaaagaggc acatctcacc
     2401 actcattggt taaacaatgc atcatagcga gcacttttcc tttccctgga gaatgggatg
     2461 tgaagcagta gaccgcagcc acgccgatgg ttatacagtg aagaagactt cacctcttcc
     2521 tattgagttt gcttggaatg ctgacagcat caggcaactc tgaactgaac atttgctttg
     2581 tcagaaaata tctttttttt tactttgaag tttggcaacc ttcatgttac cccaaagcaa
     2641 aaccattgtg tcaggagtca aacaaatgtt tagaaagcaa acatgacgtc tctattgtac
     2701 aacctccttt ctcttggctg tttaaaggat gtacttcgtg tattaaaggg tactttatgt
     2761 tgaagtaacg aaaaaaccct aatgggtgtt cctaaaccta aaccttttct ttattccaca
     2821 tttgctacgg taaaatcctg ttacaaaact accctataaa gaattatttt ctatagttaa
     2881 gcattttctg aatccta
//
