LOCUS       NM_032864               5233 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.
ACCESSION   NM_032864
VERSION     NM_032864.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Townsend,C., Leelaram,M.N., Agafonov,D.E., Dybkov,O., Will,C.L.,
            Bertram,K., Urlaub,H., Kastner,B., Stark,H. and Luhrmann,R.
  TITLE     Mechanism of protein-guided folding of the active site U2/U6 RNA
            during spliceosome activation
  JOURNAL   Science 370 (6523) (2020)
   PUBMED   33243851
  REMARK    GeneRIF: Mechanism of protein-guided folding of the active site
            U2/U6 RNA during spliceosome activation.
REFERENCE   3  (bases 1 to 5233)
  AUTHORS   Dopie,J., Sweredoski,M.J., Moradian,A. and Belmont,A.S.
  TITLE     Tyramide signal amplification mass spectrometry (TSA-MS) ratio
            identifies nuclear speckle proteins
  JOURNAL   J Cell Biol 219 (9) (2020)
   PUBMED   32609799
REFERENCE   4  (bases 1 to 5233)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 5233)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5233)
  AUTHORS   Varjosalo,M., Keskitalo,S., Van Drogen,A., Nurkkala,H.,
            Vichalkovski,A., Aebersold,R. and Gstaiger,M.
  TITLE     The protein interaction landscape of the human CMGC kinase group
  JOURNAL   Cell Rep 3 (4), 1306-1320 (2013)
   PUBMED   23602568
REFERENCE   7  (bases 1 to 5233)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
REFERENCE   8  (bases 1 to 5233)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   9  (bases 1 to 5233)
  AUTHORS   Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
            Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
  TITLE     Dynamic protein-protein interaction wiring of the human spliceosome
  JOURNAL   Mol Cell 45 (4), 567-580 (2012)
   PUBMED   22365833
REFERENCE   10 (bases 1 to 5233)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK092038.1, BU508261.1, BF223635.1, BE465896.1 and AL138849.12.
            
            On Nov 22, 2018 this sequence version replaced NM_032864.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.141408.1,
                                           SRR1660803.23158.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257181.10/ ENSP00000257181.8
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1130              AK092038.1         39-1168
            1131-1744           BU508261.1         129-742
            1745-2575           AK092038.1         1783-2613
            2576-2657           BF223635.1         65-146              c
            2658-2717           BE465896.1         9-68                c
            2718-5233           AL138849.12        3495-6010
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..5233
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="pre-mRNA processing factor 38A"
                     /db_xref="GeneID:84950"
                     /db_xref="HGNC:HGNC:25930"
                     /db_xref="MIM:617031"
     exon            1..278
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1087
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="PRP38 pre-mRNA processing factor 38 domain
                     containing A"
                     /codon_start=1
                     /product="pre-mRNA-splicing factor 38A"
                     /protein_id="NP_116253.2"
                     /db_xref="CCDS:CCDS567.1"
                     /db_xref="GeneID:84950"
                     /db_xref="HGNC:HGNC:25930"
                     /db_xref="MIM:617031"
                     /translation="MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTA
                     ELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRML
                     GALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDI
                     ILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYR
                     DLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHR
                     SRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE"
     exon            279..438
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            439..560
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            561..646
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            647..757
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            758..870
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            871..897
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            898..995
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1044
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..5233
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1393..1398
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="hexamer: ATTAAA"
     polyA_site      1418
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="major polyA site"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="hexamer: ATTAAA"
     polyA_site      2576
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
     regulatory      2692..2697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="hexamer: AATATA"
     polyA_site      2717
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
     regulatory      5203..5208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
                     /note="hexamer: AATAAA"
     polyA_site      5233
                     /gene="PRPF38A"
                     /gene_synonym="Prp38; PRP38A"
ORIGIN      
        1 acactacggt gtttccggct tcaagatggt cgcctaagct gtttagtgaa acttcttcca
       61 cctttctcca ttcctctagg tgctttttct gaacctggat gtgaggcatt aaaggatccg
      121 acggaaatag aattgaaggc attctaaaat ggctaaccgt acagtgaagg atgcgcacag
      181 catccatggc accaaccctc aatatctggt ggagaagatc attcgaacgc gaatctatga
      241 gtccaagtac tggaaagagg agtgctttgg acttacagct gaacttgtag tcgataaagc
      301 catggagtta aggtttgtgg gtggcgtcta tggtggcaac ataaaaccaa caccctttct
      361 gtgtttaacc ttgaagatgc ttcaaattca acccgagaag gatatcattg tagagtttat
      421 caaaaatgaa gatttcaagt atgtccgcat gctgggggca ctttacatga ggctgacagg
      481 cactgcaatt gattgctaca agtacttgga acctttgtac aatgactatc gaaaaatcaa
      541 gagccagaac cgaaatgggg agtttgaatt gatgcatgtt gatgagttta ttgatgaact
      601 attgcacagt gagagagtct gtgatatcat tctgccccga ctacagaaac gctatgtatt
      661 agaggaagct gagcaactgg agcctcgagt tagtgctctg gaagaggaca tggatgatgt
      721 ggagtccagt gaagaggaag aagaggagga tgagaagttg gaaagagtgc catcacctga
      781 tcaccgccgg agaagctacc gagacttgga caagccccgt cgctctccca cactgcgcta
      841 caggaggagt aggagccggt ctcccagaag gcggagtcga tctcccaaaa ggagaagccc
      901 ctcccctcgc cgagaaaggc atcggagcaa gagtccaaga cgtcaccgca gcaggtcccg
      961 agatcggcgg cacagatccc gttccaagtc cccaggtcat caccgtagtc acagacacag
     1021 gagccactca aagtctcccg aaaggtctaa gaagagccac aagaagagcc ggagagggaa
     1081 tgagtaatgg actcagtttg gttttagtcc acatggcctc ctgtggatat aaggatatct
     1141 gtatgtggaa ggattaagat ctcccccagg cagctataag aatattttag tttttttctt
     1201 atcaagtttc tcaaccttta tttttaatga aggaggtgct gagttttgta tctttttaat
     1261 cataatcaac atcagttttt gacccaacta accttgactg tattcaaact tatgagagta
     1321 taaaggatct ggaggttggg gatatgactg acaaggaaag gctgtggcca cctgatgacc
     1381 ctttcccttt ttattaaacc ggacacacct gtttcccatt tcgctgtagt ttagtttttg
     1441 gtttgttgtg gttggaactg ctttgagaat cctgggattt gtgctgctgc tgttattcaa
     1501 agatcaaagg agtaaaacat agttgctcct aacttttttc cagcagcagc aagtggtaat
     1561 aaacatgaaa actggtttgt agcagttttg aaagaataga atgcattcaa atgtaaggct
     1621 gcttctggat cattaaagcc agtttcatca aacagttcaa cagagagcag cacttaatac
     1681 cctttataca gcccattttt tcatagtttc atttgttctt gcccacaagc ttgaaatcca
     1741 ggttaaggta tccagccttt atcatataag cattgacatt atccaggcct agtcagtagc
     1801 agtagggtaa cgggattgaa aaagatttga tggagaggaa agtatctaat attagtcatg
     1861 gttttgacct aaattgctag acagtcgtgc cattcacaaa gtcagaaaat acagcaggaa
     1921 gagacagctt ttagaggggc agagaattag aggatggtgg tagtaatgaa aatgatgcat
     1981 tcagtttaac aagtttaatt tgagacagct atggtatagc taaaaacaaa agcccataaa
     2041 gttggagata gggaccagag tttaacatag cgatctaggc cagaattgac aatgtttaag
     2101 taatggtgga atctgtcaat aagacttccc agagtgttaa tatatatcag aaatgcacac
     2161 aacagaacca taggcaacac caacacagga agaaatagaa gtcacctatt gaaaactgga
     2221 atggcccatt cagaaagaca ggagaattag gagagagtga gtgaaagaag ccaagggtca
     2281 agaatgcaga agcaaaggaa ggactaaaaa gcctccacgg acatcgataa tcaagagatt
     2341 attaagatct ttgctagaag agttccttta cctgtactta actcccttaa aaagagaaaa
     2401 gtgatggaat gacttctgca actgtagtcc cagcaggaac tgtgaagacc agctgccctg
     2461 caggatccag tttttcttgg gaggttgtat ctggtcataa ggtaaacatt ctatatattc
     2521 tatgcctgct ctagaattga aagacttcag cagtattaaa gcatttttta atcttatttg
     2581 agctttcata ttcttcaatg gttcctttcg taatacctga gttaataaaa gtggtctgaa
     2641 agagctctta cggtttctga tagaactata taggactacc ttttaagatt aaatataagc
     2701 atctgtacgt aaatgtatta cagtgtcata gcagttattt ctttatgaaa gtgatctagg
     2761 tagaaagaaa tgtcttctgg catggacaga aatgacccat ggaccaacca agctgtcatc
     2821 cagtacactc ttaaaataaa actttgtctt aaaatatgtt ttaaagtaaa aataaaccca
     2881 cgcacaaagc ccaataaatt ttagtaacag aaataaacct ccctaaacaa acattaaaac
     2941 atacttgttt agacatatgg taatatgtgg accaaaattg aggtaccaca gccaatcaat
     3001 agcaggaaag agaatgtccc tattctttaa ttagaagaag gaggtaagag taagacatgt
     3061 tgcatgtctg tagatatcgt tttgataaaa ttacacagga gatggagctt gaggacaaac
     3121 cttgctgtaa ggctctttct gtcatctatg tgcttgccta tggatgaagc attttaaact
     3181 ttttgactac agcatacatt ttaaaaacgt attttacatc ttacctctgt acacatctgt
     3241 attctgtact gagacaaatt tcaggaaaga atacttacta tgcaaagtga attctggtgt
     3301 ttcctacttg ttaacagttt ttaatattgg atgaaaccac ttaatcgatt tcacttccaa
     3361 taatttgaaa tacactgact taaaagcttt gccatagggc acaatcgcct tttaatacta
     3421 agaaagtaag ggagatgtgt tccaaggggc aaaagaaata atgcattttt ggccaggtgc
     3481 ggtggctcac gcctgtaatc ccagcacttt gggaggccga ggcgggcaga tcacctgagg
     3541 tcgggagttt gagaccagcc tggccaacat ggagaaaccc tgtctctact aaaaaataca
     3601 aaattagccg ggcatggtgg cgcatgcctg taatcccagt tacttgggag gctgaggcag
     3661 gagaatcgct tgaacctggg aggcagagga tgcggtgagc tgagatcgtg ccactgcact
     3721 tcagcctggg caaaaagagc gaaactccat ctcaaaaaaa aaaaaaaaag aagaagaaga
     3781 atgggtttgg tgagagggtt ccaatattaa caacccaact tcaagctcat tcctctaacc
     3841 tcttcctgtt tgttattctt cagtagaccg aagtttaaat aagcattatt tagaaaagaa
     3901 cgatcacaga ttgcccactc cctcaaaaag gttaatgtga cagaaattct ccacctgctc
     3961 aaaaaagaca gcagacatga tctctttaaa aaaaaaaagt tcaaaagaga ttaagagatg
     4021 aaaaataaga ttggtggagt ttaaaggcct tatactagaa accacaaaac ataaaacagc
     4081 acagagaaaa taggctggag gaaataaaaa atggaaaaga gaaatatgta gaataaagaa
     4141 atattctgat ggtttaaaaa gaattggccg ggtgcggtgg ctcacgcctg taatcccagc
     4201 actttgggag gccgaggcag acggatcatg aggtcaggag atcgagacca tccaggctaa
     4261 cacagtgaaa cccgtctcta ctaaaaatac aaaaaattag ctgggtgtgc tggcgggtag
     4321 agctactagg gaggctgagg caggagaatg gcgtgaatcc gggaggcgga gctttcagtg
     4381 agccgagatc gcgccattgc actccagcct gggcaacaga gtgagactcc gtctcaaaaa
     4441 gaaaaaagaa ttaaatgggg tcaggatggt ctcagatctt ataacaagaa ggcaatgaag
     4501 caaaaggctc caaaggtttg agaaaaagtg ccaggaattt tatactttgc caaagttgtc
     4561 ttaaaataca aaggctatag atgttctcaa gtttgtaaga acttaaaagt acaaatcatg
     4621 agtctttggg aaaaaaccgc ccaataatga aattcaacta aagaagaaat gaatcaaatt
     4681 aaggacttag gaacaaagaa tcagtaaaag gagtgttagt aaacacttca gaaactttaa
     4741 aaataatggg caatgattat aaagtcaaat attatgaaca ctgtctatat agaaataaca
     4801 agtagagtgt gctggtttca tctttcatag caggaatcac gtacagtctc taattaaaac
     4861 atacatgaag ctagaaaaaa aaatagcctc tttattgttt tccgtaattg tccttaattt
     4921 taggagaaat cttacaagac tagattctct tgtggggaaa aaagtattta aattgagcat
     4981 tttttcagtt tcacacttgt tttccttttg ttaatttgct atatttgtta aatatagcat
     5041 atgagcccac ttttgtaaaa ctatttgtat atatgtgcct gtatgattaa gagagatggt
     5101 tggaatgaat gaagttcacc agatagtact tctttctggc tgatgaactt ttgaatgatt
     5161 tttaaacttt gtacttttat ataatgctta ttttcttttt acaataaaca tgttaatttc
     5221 tacaaggaag aaa
//
