LOCUS       NM_019854               2399 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens protein arginine methyltransferase 8 (PRMT8),
            transcript variant 1, mRNA.
ACCESSION   NM_019854
VERSION     NM_019854.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2399)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   4  (bases 1 to 2399)
  AUTHORS   Jeong,H.C., Park,S.J., Choi,J.J., Go,Y.H., Hong,S.K., Kwon,O.S.,
            Shin,J.G., Kim,R.K., Lee,M.O., Lee,S.J., Shin,H.D., Moon,S.H. and
            Cha,H.J.
  TITLE     PRMT8 Controls the Pluripotency and Mesodermal Fate of Human
            Embryonic Stem Cells By Enhancing the PI3K/AKT/SOX2 Axis
  JOURNAL   Stem Cells 35 (9), 2037-2049 (2017)
   PUBMED   28543863
  REMARK    GeneRIF: PRMT8 in human embryonic stem cells plays an important
            role not only in maintaining pluripotency but also in controlling
            mesodermal differentiation.
REFERENCE   5  (bases 1 to 2399)
  AUTHORS   Toma-Fukai,S., Kim,J.D., Park,K.E., Kuwabara,N., Shimizu,N.,
            Krayukhina,E., Uchiyama,S., Fukamizu,A. and Shimizu,T.
  TITLE     Novel helical assembly in arginine methyltransferase 8
  JOURNAL   J Mol Biol 428 (6), 1197-1208 (2016)
   PUBMED   26876602
  REMARK    GeneRIF: Biochemical, biophysical and mutagenesis experiments
            demonstrated that hPRMT8 forms an octamer in solution.
REFERENCE   6  (bases 1 to 2399)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
REFERENCE   7  (bases 1 to 2399)
  AUTHORS   Kim,J.D., Kako,K., Kakiuchi,M., Park,G.G. and Fukamizu,A.
  TITLE     EWS is a substrate of type I protein arginine methyltransferase,
            PRMT8
  JOURNAL   Int J Mol Med 22 (3), 309-315 (2008)
   PUBMED   18698489
  REMARK    GeneRIF: EWS is a substrate for PRMT8, as efficient as for PRMT1
REFERENCE   8  (bases 1 to 2399)
  AUTHORS   Pahlich,S., Zakaryan,R.P. and Gehring,H.
  TITLE     Identification of proteins interacting with protein arginine
            methyltransferase 8: the Ewing sarcoma (EWS) protein binds
            independent of its methylation state
  JOURNAL   Proteins 72 (4), 1125-1137 (2008)
   PUBMED   18320585
  REMARK    GeneRIF: The interaction between PRMT8 and the EWS protein was
            charcterized.
REFERENCE   9  (bases 1 to 2399)
  AUTHORS   Sayegh,J., Webb,K., Cheng,D., Bedford,M.T. and Clarke,S.G.
  TITLE     Regulation of protein arginine methyltransferase 8 (PRMT8) activity
            by its N-terminal domain
  JOURNAL   J Biol Chem 282 (50), 36444-36453 (2007)
   PUBMED   17925405
  REMARK    GeneRIF: PRMT8 N-terminal domain may function as an autoregulator
            that may be displaced by interaction with one or more physiological
            inducers.
REFERENCE   10 (bases 1 to 2399)
  AUTHORS   Lee,J., Sayegh,J., Daniel,J., Clarke,S. and Bedford,M.T.
  TITLE     PRMT8, a new membrane-bound tissue-specific member of the protein
            arginine methyltransferase family
  JOURNAL   J Biol Chem 280 (38), 32890-32896 (2005)
   PUBMED   16051612
  REMARK    GeneRIF: PRMT8 is an active arginine methyltransferase that is
            membrane-associated and tissue-specific
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA200375.1, AB209027.1 and BC022458.1.
            
            On May 17, 2019 this sequence version replaced NM_019854.4.
            
            Summary: Arginine methylation is a widespread posttranslational
            modification mediated by arginine methyltransferases, such as
            PRMT8. Arginine methylation is involved in a number of cellular
            processes, including DNA repair, RNA transcription, signal
            transduction, protein compartmentalization, and possibly protein
            translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM,
            Mar 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022458.1, SRR3476690.874141.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382622.4/ ENSP00000372067.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                DA200375.1         6-61
            57-416              AB209027.1         1-360
            417-870             BC022458.1         29-482
            871-2396            AB209027.1         4375-5900
            2397-2399           BC022458.1         2009-2011
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.32"
     gene            1..2399
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /note="protein arginine methyltransferase 8"
                     /db_xref="GeneID:56341"
                     /db_xref="HGNC:HGNC:5188"
                     /db_xref="MIM:610086"
     exon            1..498
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     CDS             424..1608
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /EC_number="2.1.1.319"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein arginine N-methyltransferase 4; heterogeneous
                     nuclear ribonucleoprotein methyltransferase-like protein
                     4; HMT1 hnRNP methyltransferase-like 3; arginine
                     methyltransferase 8"
                     /codon_start=1
                     /product="protein arginine N-methyltransferase 8 isoform
                     1"
                     /protein_id="NP_062828.3"
                     /db_xref="CCDS:CCDS8521.2"
                     /db_xref="GeneID:56341"
                     /db_xref="HGNC:HGNC:5188"
                     /db_xref="MIM:610086"
                     /translation="MGMKHSSRCLLLRRKMAENAAESTEVNSPPSQPPQPVVPAKPVQ
                     CVHHVSTQPSCPGRGKMSKLLNPEEMTSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNS
                     MYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGIECSSISDYSEKIIKANHLDN
                     IITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKPGGLMFPDR
                     AALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIK
                     EVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTH
                     WKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYK
                     MR"
     exon            499..684
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            685..840
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            841..904
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1047
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1135
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1251
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1402
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1524
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..2399
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2378..2383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /note="hexamer: AATAAA"
     polyA_site      2399
                     /gene="PRMT8"
                     /gene_synonym="HRMT1L3; HRMT1L4"
                     /note="major polyA site"
ORIGIN      
        1 gcttcgccca gcggatcggc agaagttgag aggagttggc ggctgcctcc ggccggccgg
       61 actttgcgag cagcctggag aggatccgcg accgccgccg ccgccgccgc ggaggcttcg
      121 gggctgcttc cctcgagctt agcccgcagc gcgggtggag aggggcgggg agggggtcgg
      181 gggcacgaga agaacttgaa accgtgtgaa ggaatccgga gcagatgaga agggaggaaa
      241 ataaaagaaa gtggagactg cagaacagac tccgctgtgg ctgactgtgc cggccgacgc
      301 tccagctgag gggctgggtt ggattttttt ttttctccca tcctctcgct ctctctttta
      361 aagcgacacc agctctctct cctcctctac tatctcggta tcaccaaacc cttgccggct
      421 cttatgggca tgaaacactc ctcccgctgc ctgctcctga ggaggaaaat ggcggagaac
      481 gcggccgaga gcaccgaggt gaacagcccc ccctcccagc ccccccagcc cgtcgtccct
      541 gctaagcccg tgcaatgcgt ccatcatgtg tccactcaac ccagctgccc aggacggggc
      601 aagatgtcca agctgctgaa cccagaggag atgacctcga gagattatta cttcgactcc
      661 tatgcccact ttgggatcca cgaggaaatg ctgaaggatg aggtgcggac tctcacttac
      721 cggaactcca tgtaccacaa caagcacgtg ttcaaggaca aagtggtact ggatgtgggg
      781 agtggtactg ggatcctttc catgttcgct gccaaggcag gggccaagaa ggtgtttggg
      841 atcgaatgct ccagtatttc tgactactca gagaagatca ttaaggccaa ccacttggac
      901 aacatcatca ccatatttaa gggtaaagtg gaagaggtgg agctgcctgt ggagaaggtg
      961 gacatcatca tcagcgagtg gatgggctac tgtctgttct atgagtccat gctcaacacg
     1021 gtgatctttg ccagggacaa gtggctgaaa cctggagggc ttatgtttcc agaccgggca
     1081 gctttgtacg tggtagcgat tgaagacaga cagtacaagg acttcaaaat ccactggtgg
     1141 gagaatgtct atggctttga catgacctgc atccgggacg tggccatgaa ggagcctcta
     1201 gtggacatcg tggatccaaa gcaagtggtg accaatgcct gtttgataaa ggaggtggac
     1261 atttacacag tgaagacgga agagctatcg ttcacatctg cattctgcct gcagatacag
     1321 cgcaacgact acgtccacgc cctggtcacc tattttaata ttgaatttac caagtgccac
     1381 aagaaaatgg ggttttccac agcccctgat gctccctaca cccactggaa gcagaccgtc
     1441 ttctacttgg aagattacct cactgtccgg aggggggagg aaatctacgg gaccatatcc
     1501 atgaagccaa atgccaaaaa tgtgcgagac ctcgatttca cagtagactt ggattttaag
     1561 ggacagctgt gtgaaacatc tgtatctaat gactacaaaa tgcgttagca cacgtgggaa
     1621 gctgcagaga gcaacgagaa aaggaactct cacctcgatc tgccgtgccg tcccaaagaa
     1681 taccgtttgc aggactacac acttgaaaac cagagttttc aactctgcct tgaagattgg
     1741 tgaactcccc agggctcccg tgggctctgc cactggacag aaggcctcca gctcctccgc
     1801 tctgccctgg tagcccttca cgaaggcttt gtgttgccaa caaagagcga cctggcgtgc
     1861 tgtggctggg ccccgagggt ggaaacgtat tcgcgtctcc ccgtctcctc cttaactgtg
     1921 actctccggg tcttctgagt tttgcatgct gcgggtgtct aggacagatt gcttccacta
     1981 gaacctggag acatagcatc tttgatagca taagccagat tatctgtgtg tgcggtggtg
     2041 tgcgtgtgcg tgcatgtgtg aatgtgagca gcatagttga tatttaccca caaacacctg
     2101 tatatgcgtg catatacaac caagtgggta gacctaggtg ttctctcaga ggggtgtgtg
     2161 tgtgtgtgcg tgcgcgtgtg cctagaatat atattactct cagaggagat tctgttgctt
     2221 ttgaatagga atttgttttg tgattagttc gccccttccc caccccttac cagatgttaa
     2281 gcagctatga aacattctct gtactagttc tggtctcctt ttgactggac tgtggctctg
     2341 aaccttgagc atagtaccac ggactccgtg ggcgctcaat aaacacacat gagaacaaa
//
