LOCUS       NM_000949              11581 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens prolactin receptor (PRLR), transcript variant 1, mRNA.
ACCESSION   NM_000949
VERSION     NM_000949.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Shen,S., Radhakrishnan,S.K., Harrell,J.C., Puchalapalli,M.,
            Koblinski,J. and Clevenger,C.
  TITLE     The Human Intermediate Prolactin Receptor I-tail Contributes Breast
            Oncogenesis by Targeting Ras/MAPK Pathway
  JOURNAL   Endocrinology 165 (6) (2024)
   PUBMED   38713636
  REMARK    GeneRIF: The Human Intermediate Prolactin Receptor I-tail
            Contributes Breast Oncogenesis by Targeting Ras/MAPK Pathway.
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Amin,M. and Gragnoli,C.
  TITLE     The prolactin receptor gene (PRLR) is linked and associated with
            the risk of polycystic ovarian syndrome
  JOURNAL   J Ovarian Res 16 (1), 222 (2023)
   PUBMED   37993904
  REMARK    GeneRIF: The prolactin receptor gene (PRLR) is linked and
            associated with the risk of polycystic ovarian syndrome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11581)
  AUTHORS   Araya-Secchi,R., Bugge,K., Seiffert,P., Petry,A., Haxholm,G.W.,
            Lindorff-Larsen,K., Pedersen,S.F., Arleth,L. and Kragelund,B.B.
  TITLE     The prolactin receptor scaffolds Janus kinase 2 via co-structure
            formation with phosphoinositide-4,5-bisphosphate
  JOURNAL   Elife 12, e84645 (2023)
   PUBMED   37232489
  REMARK    GeneRIF: The prolactin receptor scaffolds Janus kinase 2 via
            co-structure formation with phosphoinositide-4,5-bisphosphate.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11581)
  AUTHORS   Begon,E. and Bernard,V.
  TITLE     [Prolactin and its receptor: From animal models to pituitary
            pathophysiology]
  JOURNAL   Biol Aujourdhui 216 (3-4), 105-110 (2022)
   PUBMED   36744975
  REMARK    GeneRIF: [Prolactin and its receptor: From animal models to
            pituitary pathophysiology].
REFERENCE   5  (bases 1 to 11581)
  AUTHORS   Trott,J.F., Hovey,R.C., Koduri,S. and Vonderhaar,B.K.
  TITLE     Alternative splicing to exon 11 of human prolactin receptor gene
            results in multiple isoforms including a secreted prolactin-binding
            protein
  JOURNAL   J Mol Endocrinol 30 (1), 31-47 (2003)
   PUBMED   12580759
REFERENCE   6  (bases 1 to 11581)
  AUTHORS   Kline,J.B., Rycyzyn,M.A. and Clevenger,C.V.
  TITLE     Characterization of a novel and functional human prolactin receptor
            isoform (deltaS1PRLr) containing only one extracellular
            fibronectin-like domain
  JOURNAL   Mol Endocrinol 16 (10), 2310-2322 (2002)
   PUBMED   12351696
  REMARK    GeneRIF: Given its apparent widespread expression, this PRLr
            isoform may contribute to PRL action.
REFERENCE   7  (bases 1 to 11581)
  AUTHORS   Kline,J.B., Roehrs,H. and Clevenger,C.V.
  TITLE     Functional characterization of the intermediate isoform of the
            human prolactin receptor
  JOURNAL   J Biol Chem 274 (50), 35461-35468 (1999)
   PUBMED   10585417
REFERENCE   8  (bases 1 to 11581)
  AUTHORS   Rozakis-Adcock,M. and Kelly,P.A.
  TITLE     Identification of ligand binding determinants of the prolactin
            receptor
  JOURNAL   J Biol Chem 267 (11), 7428-7433 (1992)
   PUBMED   1559983
REFERENCE   9  (bases 1 to 11581)
  AUTHORS   Di Carlo,R., Muccioli,G., Papotti,M. and Bussolati,G.
  TITLE     Characterization of prolactin receptor in human brain and choroid
            plexus
  JOURNAL   Brain Res 570 (1-2), 341-346 (1992)
   PUBMED   1617425
REFERENCE   10 (bases 1 to 11581)
  AUTHORS   Arden,K.C., Boutin,J.M., Djiane,J., Kelly,P.A. and Cavenee,W.K.
  TITLE     The receptors for prolactin and growth hormone are localized in the
            same region of human chromosome 5
  JOURNAL   Cytogenet Cell Genet 53 (2-3), 161-165 (1990)
   PUBMED   2369845
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP357907.1, AK313270.1,
            AC010368.4, KF573643.1 and AI079645.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000949.6.
            
            Summary: This gene encodes a receptor for the anterior pituitary
            hormone, prolactin, and belongs to the type I cytokine receptor
            family. Prolactin-dependent signaling occurs as the result of
            ligand-induced dimerization of the prolactin receptor. Several
            alternatively spliced transcript variants encoding different
            membrane-bound and soluble isoforms have been described for this
            gene, which may function to modulate the endocrine and autocrine
            effects of prolactin in normal tissue and cancer. [provided by
            RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript, and encodes the longest isoform (1, also known as long
            form).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059392.1, M31661.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618457.5/ ENSP00000482954.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-371               BP357907.1         103-473
            372-2194            AK313270.1         362-2184
            2195-8408           AC010368.4         77012-83225
            8409-8840           KF573643.1         1-432               c
            8841-11234          AC010368.4         83655-86048
            11235-11581         AI079645.1         12-358              c
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.2"
     gene            1..11581
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="prolactin receptor"
                     /db_xref="GeneID:5618"
                     /db_xref="HGNC:HGNC:9446"
                     /db_xref="MIM:176761"
     exon            1..220
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            221..282
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            283..395
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     CDS             326..2194
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; hPRL receptor; secreted prolactin binding
                     protein"
                     /codon_start=1
                     /product="prolactin receptor isoform 1 precursor"
                     /protein_id="NP_000940.1"
                     /db_xref="CCDS:CCDS3909.1"
                     /db_xref="GeneID:5618"
                     /db_xref="HGNC:HGNC:9446"
                     /db_xref="MIM:176761"
                     /translation="MKENVASATVFTLLLFLNTCLLNGQLPPGKPEIFKCRSPNKETF
                     TCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMM
                     VNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLK
                     TGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAW
                     SPATFIQIPSDFTMNDTTVWISVAVLSAVICLIIVWAVALKGYSMVTCIFPPVPGPKI
                     KGFDAHLLEKGKSEELLSALGCQDFPPTSDYEDLLVEYLEVDDSEDQHLMSVHSKEHP
                     SQGMKPTYLDPDTDSGRGSCDSPSLLSEKCEEPQANPSTFYDPEVIEKPENPETTHTW
                     DPQCISMEGKIPYFHAGGSKCSTWPLPQPSQHNPRSSYHNITDVCELAVGPAGAPATL
                     LNEAGKDALKSSQTIKSREEGKATQQREVESFHSETDQDTPWLLPQEKTPFGSAKPLD
                     YVEIHKVNKDGALSLLPKQRENSGKPKKPGTPENNKEYAKVSGVMDNNILVLVPDPHA
                     KNVACFEESAKEAPPSLEQNQAEKALANFTATSSKCRLQLGGLDYLDPACFTHSFH"
     exon            396..528
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            529..698
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            699..868
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1010
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1110
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1180
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..11581
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11403..11408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="hexamer: ATTACA"
     polyA_site      11421
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="major polyA site"
     regulatory      11561..11566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="hexamer: AATAAA"
     polyA_site      11581
                     /gene="PRLR"
                     /gene_synonym="HPRL; hPRLrI; MFAB; RI-PRLR"
                     /note="major polyA site"
ORIGIN      
        1 gcttcttctc acagagccac cgggctgggg accggggagc ggaggctgaa atccccagac
       61 gccggttttc tgggctgggc tttctgcctt actcactcct tctccctctt tctggatttt
      121 accgaccgtt cgcgaaacag ctttccacac aatggagctt catgtcctcg tgcaggaagt
      181 actcatcgac tgatgtggca gactttgctc cctggcaaaa ctaaagaact ctcctattca
      241 tggaggcgaa cactgaggat gctttccaca tgaaccctga agtgaacttc tgatacattt
      301 cctgcagcaa gagaaggcag ccaacatgaa ggaaaatgtg gcatctgcaa ccgttttcac
      361 tctgctactt tttctcaaca cctgccttct gaatggacag ttacctcctg gaaaacctga
      421 gatctttaaa tgtcgttctc ccaataagga aacattcacc tgctggtgga ggcctgggac
      481 agatggagga cttcctacca attattcact gacttaccac agggaaggag agacactcat
      541 gcatgaatgt ccagactaca taaccggtgg ccccaactcc tgccactttg gcaagcagta
      601 cacctccatg tggaggacat acatcatgat ggtcaatgcc actaaccaga tgggaagcag
      661 tttctcggat gaactttatg tggacgtgac ttacatagtt cagccagacc ctcctttgga
      721 gctggctgtg gaagtaaaac agccagaaga cagaaaaccc tacctgtgga ttaaatggtc
      781 tccacctacc ctgattgact taaaaactgg ttggttcacg ctcctgtatg aaattcgatt
      841 aaaacccgag aaagcagctg agtgggagat ccattttgct gggcagcaaa cagagtttaa
      901 gattctcagc ctacatccag gacagaaata ccttgtccag gttcgctgca aaccagacca
      961 tggatactgg agtgcatgga gtccagcgac cttcattcag atacctagtg acttcaccat
     1021 gaatgataca accgtgtgga tctctgtggc tgtcctttct gctgtcatct gtttgattat
     1081 tgtctgggca gtggctttga agggctatag catggtgacc tgcatctttc cgccagttcc
     1141 tgggccaaaa ataaaaggat ttgatgctca tctgttggag aagggcaagt ctgaagaact
     1201 actgagtgcc ttgggatgcc aagactttcc tcccacttct gactatgagg acttgctggt
     1261 ggagtattta gaagtagatg atagtgagga ccagcatcta atgtcagtcc attcaaaaga
     1321 acacccaagt caaggtatga aacccacata cctggatcct gacactgact caggccgggg
     1381 gagctgtgac agcccttccc ttttgtctga aaagtgtgag gaaccccagg ccaatccctc
     1441 cacattctat gatcctgagg tcattgagaa gccagagaat cctgaaacaa cccacacctg
     1501 ggacccccag tgcataagca tggaaggcaa aatcccctat tttcatgctg gtggatccaa
     1561 atgttcaaca tggcccttac cacagcccag ccagcacaac cccagatcct cttaccacaa
     1621 tattactgat gtgtgtgagc tggctgtggg ccctgcaggt gcaccggcca ctctgttgaa
     1681 tgaagcaggt aaagatgctt taaaatcctc tcaaaccatt aagtctagag aagagggaaa
     1741 ggcaacccag cagagggagg tagaaagctt ccattctgag actgaccagg atacgccctg
     1801 gctgctgccc caggagaaaa ccccctttgg ctccgctaaa cccttggatt atgtggagat
     1861 tcacaaggtc aacaaagatg gtgcattatc attgctacca aaacagagag agaacagcgg
     1921 caagcccaag aagcccggga ctcctgagaa caataaggag tatgccaagg tgtccggggt
     1981 catggataac aacatcctgg tgttggtgcc agatccacat gctaaaaacg tggcttgctt
     2041 tgaagaatca gccaaagagg ccccaccatc acttgaacag aatcaagctg agaaagccct
     2101 ggccaacttc actgcaacat caagcaagtg caggctccag ctgggtggtt tggattacct
     2161 ggatcccgca tgttttacac actcctttca ctgatagctt gactaatgga atgattggtt
     2221 aaaatgtgat ttttcttcag gtaacactac agagtacgtg aaatgctcaa gaatgtagtc
     2281 agactgacac tactaaagct cccagctcct ttcatgctcc atttttaacc acttgcctct
     2341 ttctccagca gctgattcca gaacaaatca ttatgtttcc taactgtgat ttgtagattt
     2401 actttttgct gttagttata aaactatgtg ttcaatgaaa taaaagcaca ctgcttagta
     2461 ttcttgaggg acaatgccaa taggtatatc ctctggaaaa ggctttcatg atttggcatg
     2521 ggacagacgg aaatgaaatt gtcaaaattg tttaccatag aaagatgaca aaagaaaatt
     2581 ttccacatag gaaaatgcca tgaaaattgc ttttgaaaaa caactgcata acctttacac
     2641 tcctcgtcca ttttattagg attacccaaa tataaccatt taaagaaaga atgcattcca
     2701 gaacaaattg tttacataag ttcctatacc ttactgacac attgctgata tgcaagtaag
     2761 aaataatgcc tccaattttt tcttaaaata gtttagatat gttaagcatg gattttcccc
     2821 ctaaattcta ctctaaagct tctataaaat atagtttgga aataaaaatg ttcttacaca
     2881 aggggcaaat gaatttcatg tgaatccctt taggatgttt tcctccttca tttcagcatg
     2941 atagataatt tattatacac cttagtcttt ccttagttaa aaaaaaattc cctaaagact
     3001 aacttgaaaa gaaaaccaca gcaggatgct aatggaatta ctgccttact gtacatacaa
     3061 attctacttt aatataaacc cataagttta actgtatttt tgaagactat ttttctatga
     3121 cttaggtaaa ataaaagact attttctatt ttcccagtac agctgtttat gtattcagtg
     3181 ggtacagtat tttctgctgc aaggttataa tgtgtattta cagtacatag tagtatataa
     3241 agttcccgtt tgtggagtaa aatgccatac caacccaaag cccactgtga aatatgaagt
     3301 gaatgacaaa attcagtttt caacgcaata tagtatagtt tatctgattc ttttgatctc
     3361 caggacactt taaacaactg ctaccaccac caccaaccta gggatttagg attctccaca
     3421 gaccagaaat tatttctcct ttgagtttca ggctcctctg ggactcctgt tcatcaatgg
     3481 gtgaatcttc ctaaagctac cataaaaagg gagggcgacg atttctaaca gctgtttaaa
     3541 agagagaggt tttgcttttg tgagaaatct tgggccacaa aaaaatttta agcaaagcca
     3601 ggagttagtg tctctctgaa gtaaattcaa ttgcaaaatc ttggcagagg cagaaatggc
     3661 tcatatcaga agtgtccaaa taaaagttct gggaaactag gtatttgctt cccagagcaa
     3721 tgggaatagc tccaaaggtc agagtaaggt aatgagaatc tcccggtcaa ttccagtgcc
     3781 aggcttcctg aaagaagtca aagcttggat cctcatttta aagggcgcat ccatgactca
     3841 tctagcagcc cctctctatt tttcccttca cttgatggta aatccattct atatgtccat
     3901 ttactagctg gacatagaga acttgagctc acctctggga gttaaatatt ccaccccaac
     3961 tatcccatat tgatagcaac tggactcaga ggtccccctc ctgtttgaaa gtgaacacat
     4021 tgccaatgtt ttgagaatgg caaaagcaat agatacgaag ctccattttt ctaatatata
     4081 aacaatatca tgtgagtaag ggaagcgtgt gggaaagttg tccaagctca ttcagtcccc
     4141 taaatcctcg atactttaac tgtctgagaa ccaactgcat aggcatcacc tgggcagtgt
     4201 ttgaaaattc catttgcaga atcttaagca tcagaatctg cattttacta agatccccaa
     4261 atgattcaat tgcacactga agtttgagaa gcattgttct agacaaccat ttccactaaa
     4321 ggttatttta gttcatagta acaatcctct tttggaaagg acagatcaag tctgtttgac
     4381 ttgcaatgaa caacactgca tgtaagcagg caaaaaaaga aacatatcag tagtttaaga
     4441 ttcacattat gctgaatctt atatgaattc acataaaaga atgacctgca aaactacgca
     4501 caacttctga aaggccaagg aatataatct accttgagag acattaacca aagacagtca
     4561 ccctcaaagc caatgcagac taaatctttc ctaacatagt ctattctcta agaatagaga
     4621 agatatgtat cagtcactct tatagtctga aaacctaaac ataaaagtaa tctgcttttg
     4681 acatataatt ttgtataata cgtatgaaaa aatgtgacag ctaaggactt gaattaaatc
     4741 cctaaaaaac aattagccac tcattaggcc aagactttaa ttctatgatc aattagcact
     4801 gtttatctga attaagcttt tttgtggtct aacaaaaagt ttttttgtgg tctatcaagg
     4861 agacagctgt tcaaatggaa ggatacatgt ggggcagggg ggcggtgaag agacctagat
     4921 tctggtccta actgctttgc cttggagaga ttaattcccc cctctaagcc tcagtttcct
     4981 ctgaagatgt tggaccagat aacctctaag gtcctttgaa actgactcct tgattccatg
     5041 actcactgaa tatttttctg caaagataat cccaaccaat gccttctatc ctaatctaac
     5101 aaaaaattgc tcttcatcat agaaatacaa agtaatcttc agtgatgtgc tctttatact
     5161 ttgcttttta aaccgtccat tccagttggt cttaatatat tgcacaagta ttttataagt
     5221 gaaatgtgta cagaatcata ttctaagatt atgtgcaaat atatgtgtag aaagaggcat
     5281 ttaaaaaaag aggcaggaga ttgagaagag acatattgaa tgaggcacta atctttagaa
     5341 aaatgatctt ttactgaatc aagccatgct cattccttgc tgaatccaac agccccaaat
     5401 ctcttttctt atatttggct gttaattttt ttccttgcac catcctagat tgttaacaac
     5461 cattgcacag ttggctcatg tatccttaca ctctgagtta taataagttg tatttttata
     5521 aacaaatacc taaatattca agttttaagg cgcatggaaa ctgaatctag gcaacgggtg
     5581 atttgtgtag cttacaacct gaacagcaag atgttccaat tcattctaac agatgtgtat
     5641 aagcacaagt tatatcagct gtgtcagata tttaacagat cctacattta ttagctatat
     5701 aacagactct acattttcct aagtgcctaa gaccaatcct ggtagtcact taagtacaaa
     5761 agactcactt taactggctt ggtctgaagg ggtcaatcct ttactgcctg tacaagatct
     5821 gggcagtttt gctctgtgtc ttgtgatgac ccagctgaat gtgaaaagcc tatgagttat
     5881 tgagcaacca gaggtccctg cattccaagt tgcagtttag ctctggggaa actctggctt
     5941 tctctttcct caagtgtcca ccactgccca tcatttgcag aaagacttgg ccctgccttt
     6001 agcaacttcc ctatctcctt agagtagaca agaaagaaac agatttgtgc acattttaca
     6061 aagtgccttc ctctattttt ttttaaagtt ttcattttgc aaggaaaaat gtattgatta
     6121 gaaagtaaca tttgccttat agggattata tatatatata gatatagata tagatatgta
     6181 tgaatggaga gatgagaaga agccagaaag ggcacaacaa aatggtctaa gggccaacca
     6241 gcaatgttca gtgaatgttt acgtattgtt taattgaatc aacacagagc tgttgtgaat
     6301 agtgtgactt actttgcctg ataagaaatt gaggctggct gtgatttggg gataaacctg
     6361 ccaccctgtg atttgagact ctccatacca aaacttattg atacattttt gaatgtcatt
     6421 ggcttacatg cctaaacctg aagagaacaa tgcatgcaca ttggaacttt gtacacaaac
     6481 agaaaaatat aaatgacttt gaattaccat cccaagcatt gctaacgtag atgtgccatt
     6541 gacgaaagtc tcatgtgttc ttgagtgaac aaatttcctt ttcttccttt ttggctcaag
     6601 cacaataatg ctgtcactga gacagtgact gagcactcaa tgtccaagac taaatcacat
     6661 caccacgact aagtaggcac tatgtcctgg gatgagtctt ttaaactctg gccatcaggt
     6721 tccttacttg ttaaataagt aggttggcct aaaaggtttc taagacctct catgatctag
     6781 gtaaaatcca gggtttcagg tcagtgtccc tggtgcagtg caagaaagcc ttgctgacac
     6841 acccttggcc tgaagcagtg agcacatgag attgtgtgac aacgcttcag agaaaaacag
     6901 agatctgttc ctccaactca gattgattta acatccttgc ttctcctttt ccttcctggc
     6961 tttacatatt tgtagggttt ttactccttc tccagagcct actattttcc taagagttag
     7021 tagcaaccat aggatccaca agaaatgaaa ttattcatca acaatgactt tggcactgca
     7081 aaagactgag tcaaaggtta aatcagtcac acagatatcc aaggccaagt tgaagatcac
     7141 aagtatatcc catttagaga tttgcatact tagagattaa gcccatgttc attaattatt
     7201 tagttaattc aaaagccatt aataaatcca tgcccagcct acgcaacata gtaagaccca
     7261 gtctctacaa aaattttaaa aattagccag ccatggtgac acgtacctgt agtcctagac
     7321 acttgggaag gtgaagccag aggattgctt gagcccagga ggtcgaggct gcagtgaacc
     7381 gtgattgtgt cactgcactc cagcctgggt gagagagcaa gaccttgtct caataaataa
     7441 acccaagtta cttcgatgac tagatactgg aggtctgaag gagaagcttt aaaaatacag
     7501 attgattgct tcagagaaat cctatttata attatgatct gaacttattc tatgattagc
     7561 aaatatatca tctgaaaata aaacaggagt tctctcccca gacttttgtt tttcaggatg
     7621 gtgagttttg gtctgggacc ctcatggcac ccattttgat aaaaggaaaa agatagtaaa
     7681 acaggaaata ggaagtttcc agagatttgg gcatctcagg attccatgaa atgctgagac
     7741 taacccacta aatccaaatt ttattggcta tggtaaatct aaatctgttc agtgttctga
     7801 acagatttta tgagtttatg tctaagagtt tatgtctaag agaggtacat aattcacaaa
     7861 cactatcatg ctggcagctg ttttgtccac atcaattccc ttgatttaat gctctgattt
     7921 caatttagaa agtactgaga catgtaaagt ctcaaaatag gaaaaaccta tcctgtttat
     7981 aaggtgtggt caataaatag accacagata atcttagtct attaattaac tctcagatca
     8041 tcttagacta ttaattaaca ttctgcttgc tgctgattaa aaagaaaaaa accctaaaac
     8101 cttcagcaac tgaagtagta atataaaaaa taacctttaa tagttatggt caaattacgc
     8161 ttcacttgga taaacctcaa aatgtagtct gagttttttt gtgcacactt cacaaatcct
     8221 gggggaaaaa tcttttttcc acgttatctg aaaaactata tggcgaagat accgctgaaa
     8281 aataatttgt tttatgaaat tcctgaaaag ctaattttga attttttaaa gtaactacct
     8341 cccttttgtg tcctaatgtc cttccttcaa agctgcttat agtttagaaa tgaaagatgt
     8401 catgtctcat gctttgactg gttttcggtg gcatatttga tgtatttgcc aagtggcatt
     8461 actgacttta cagtaactgt tacattattt tgtactatat tgctttatgt ggttcaaaaa
     8521 tagtatatgc catattgaaa tatgtaatcc tcatgttgtg aaactcatca tgtaatacaa
     8581 atgaaaatca ttacttaata acttacaatt tatccatatg tagtttcaat atgattgtgt
     8641 ctatgactgt aaaacaaaac tgaaaaaata tgtaatgtat ttctgttgaa agcgtgaatg
     8701 tactaatttt ttcctaatct ttgtctagaa gaaagattct atgtcattca ttgtacagct
     8761 gctctggggt gttacacatt taaagtacaa tccaacagtt gtttgaaagt cagcctctcg
     8821 gctttgtttc atgtgactgc tgacacttct caagtgttct ttgcataatg aggctggatt
     8881 ttgtgcttca tcgttcattt taaacaattg aaaagtctca tgtggaaacc aaagcaatct
     8941 agaaccagcc tgttgggtga ctgtgtcttc tgtaaagcca tattgaagtg aagttataca
     9001 taatagcata aggttccaca ttttcaaagc taccactacc tctgcttttc ctctttccct
     9061 agaaaatgcc aagcaaaatt ccccaacact aagtgagaaa ttagaagtaa ttgttttata
     9121 attaagttgc tgggtttttt ttttaacacc ttctaattca tcactttgta gggttatgca
     9181 agagtttctg aataaccatt cttacatgtt tatattttat aaaaaccggc tgcaagaaag
     9241 attcagaaag aatacttagt aagtcatcat atgaaaaacc aacaaaacca tagagtgcag
     9301 attttttcct aactataagt cacctcatag ttgtccaaag ccatgataat gatatctctt
     9361 acaaattgac ttctgtaacc cccctagaaa taaccctttt ccttatttga ttttagtcat
     9421 caaacatagt atgatatggg aaaagtcagc catttaccag aaattatctt attttgattt
     9481 taaaaactca tttctatatg tagttattgt aatgtctatt tttttagact taaagattta
     9541 tagaagacta tagttatctg atttgttatt ttgcattttt cattctgtaa atctttgctt
     9601 atggcacatt gtgctctctg ttttccatgg ttttattcat ttatctcctc ctattttgag
     9661 gggacaacat gggtagttaa atctttgtca atagtattgg agataacact aactgctatt
     9721 atcataacat cttcattttt actgcatgcc aaaaccaatg cctgccaaac aaaatcttag
     9781 acatcccaat ataatatgtt agttatattt ctattcacat cattattgaa aatacccagc
     9841 tcagtgcctg gcttaataaa tgtttaattc ccttacctac tcttgctcta tttttttatt
     9901 tgaaatggag atgagcaaaa taacacattc atggctgaag caattttttg gacatttctt
     9961 gttaccaaaa gatctataat caggacattg tttatgtaag ttggacaaaa aaaattcttc
    10021 ccctttatgt ccacccttcc tatgattgca agacaaaatt tccctccttt acctcatccc
    10081 tataacatgg gaggctgaga aaaatgaggg gagatggaac cagatacaag gagatccaat
    10141 aagagaagct tatttaaata ttgtgaaata aaggaagaac caaagcattt ttttaagtgg
    10201 ggaatccttt tgaacagtta ttatttatcc atattattaa caacatcttt tctgacaaaa
    10261 tccatcagat gaagtgtaaa tggataatct tttaatggat ctaaacctag aaagtttcac
    10321 ttactgttca tgtccgtgtt ccagaattgt gaaatggtgt gtggttttgc tttccaagtt
    10381 cttctctgcc tcctcttaat tctctaattc catgtcttac agaagaatga gaaatttctt
    10441 tcttacttga gtatcatgct ctaaaaaact tggcttcagt cacagaaacg ctggctctcc
    10501 tgtgcttata ttgaagccaa ctgcctttaa ttcttgggcc ctcttatatt tttaaggtgc
    10561 aaaatttgaa gtctcagtca ccagacacag gttctataca attaatgatg agctggagaa
    10621 gtaatatgta gctaattttt caaaagcatt gaatatactt tccggaaaga aaacagaaat
    10681 taaatattgc cacatcttgc cagaatccca tctgacacct taactttgtc aggtttccta
    10741 caacttgcta atcaagtttt atacattcta aatctcccca gtttctttgg ggctggaaga
    10801 tgcaacttcc atttaataga aactttgaaa tcttggggta agggagcagt ggggggacta
    10861 gggagaagga taagaaatag aattattgaa aagcccccac cagggacctt cctggccaga
    10921 atatgcagag taattcctgc tggcttcacc tttgaaagtc cctcgaaact atgcagatga
    10981 aactgagtct gtttttgata ttgtcagatg tattctacct tggaagtccc aacacctaaa
    11041 ctggaattct tgtatttaca tctcctccac tgtcccccac accacccctc aattcctgct
    11101 gcccctgcta atgttaagca tttttctctt gttatcatca ggttcacatt aaaaacagat
    11161 acttacaaac tgacttgaag cacagatact tttacgaatg tgataaaata ttttcttaag
    11221 aaaaggaaag aggatgtggg tcaaataaaa caccgcatgg atgttgattg gtgaatactg
    11281 gtgtaagaaa agggagctca ggaattttta ttactgtatt tgtaaatgag tttgaaggaa
    11341 tttgtaaatg ccactggtac atttttaagg tgacacattt gctccttata aagttattaa
    11401 aaattacagg gtaagcttaa atgacgtttg ccagtagttt tactttatat aatcaatatt
    11461 gatattgttg ctgaactatg taactttatg atgcattttt cagtcccttt tcagagcaaa
    11521 tgcttttgca atggtagtaa tgtttagttt aaattgactt aataaattat tacctgagca
    11581 a
//
