LOCUS       NM_003690               1770 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens protein activator of interferon induced protein kinase
            EIF2AK2 (PRKRA), transcript variant 1, mRNA.
ACCESSION   NM_003690
VERSION     NM_003690.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Shivaprasad,S., Qiao,W., Weng,K.F., Umashankar,P., Carette,J.E. and
            Sarnow,P.
  TITLE     CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral
            Replication
  JOURNAL   Viruses 16 (5), 725 (2024)
   PUBMED   38793607
  REMARK    GeneRIF: CRISPR Screen Reveals PACT as a Pro-Viral Factor for
            Dengue Viral Replication.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Lin,Y.Y., Yao,R., Zhuang,J., Wang,B., Du,L., Guo,D., Pan,J.A. and
            Peng,X.
  TITLE     PACT inhibits the replication of SARS-CoV-2 through the blockage of
            GSK-3beta-N-nsp3 cascade
  JOURNAL   J Med Virol 95 (6), e28832 (2023)
   PUBMED   37264691
  REMARK    GeneRIF: PACT inhibits the replication of SARS-CoV-2 through the
            blockage of GSK-3beta-N-nsp3 cascade.
REFERENCE   3  (bases 1 to 1770)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   4  (bases 1 to 1770)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   5  (bases 1 to 1770)
  AUTHORS   Wei,L., Wang,W., Yao,J., Cui,Z., Xu,Z., Ding,H., Wu,X., Wang,D.,
            Luo,J. and Ke,Z.J.
  TITLE     PACT promotes the metastasis of basal-like breast cancer through
            Rac1 SUMOylation and activation
  JOURNAL   Oncogene 41 (37), 4282-4294 (2022)
   PUBMED   35974143
  REMARK    GeneRIF: PACT promotes the metastasis of basal-like breast cancer
            through Rac1 SUMOylation and activation.
REFERENCE   6  (bases 1 to 1770)
  AUTHORS   Horng,T., Barton,G.M. and Medzhitov,R.
  TITLE     TIRAP: an adapter molecule in the Toll signaling pathway
  JOURNAL   Nat Immunol 2 (9), 835-841 (2001)
   PUBMED   11526399
REFERENCE   7  (bases 1 to 1770)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 1770)
  AUTHORS   Ito,T., Yang,M. and May,W.S.
  TITLE     RAX, a cellular activator for double-stranded RNA-dependent protein
            kinase during stress signaling
  JOURNAL   J Biol Chem 274 (22), 15427-15432 (1999)
   PUBMED   10336432
REFERENCE   9  (bases 1 to 1770)
  AUTHORS   Patel,R.C. and Sen,G.C.
  TITLE     PACT, a protein activator of the interferon-induced protein kinase,
            PKR
  JOURNAL   EMBO J 17 (15), 4379-4390 (1998)
   PUBMED   9687506
REFERENCE   10 (bases 1 to 1770)
  AUTHORS   Simons,A., Melamed-Bessudo,C., Wolkowicz,R., Sperling,J.,
            Sperling,R., Eisenbach,L. and Rotter,V.
  TITLE     PACT: cloning and characterization of a cellular p53 binding
            protein that interacts with Rb
  JOURNAL   Oncogene 14 (2), 145-155 (1997)
   PUBMED   9010216
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG827125.1, AK290601.1 and
            AC009948.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003690.4.
            
            Summary: This gene encodes a protein kinase activated by
            double-stranded RNA which mediates the effects of interferon in
            response to viral infection. Mutations in this gene have been
            associated with dystonia. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Nov 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.362406.1,
                                           SRR3476690.1174409.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325748.9/ ENSP00000318176.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BG827125.1         58-75
            19-900              AK290601.1         1-882
            901-901             AC009948.3         42763-42763         c
            902-1705            AK290601.1         884-1687
            1706-1770           AC009948.3         38004-38068         c
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..1770
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="protein activator of interferon induced protein
                     kinase EIF2AK2"
                     /db_xref="GeneID:8575"
                     /db_xref="HGNC:HGNC:9438"
                     /db_xref="MIM:603424"
     exon            1..210
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1087
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein activator of the interferon-induced protein
                     kinase; PKR-associated protein X; PKR-associating protein
                     X; interferon-inducible double-stranded RNA-dependent
                     protein kinase activator A; protein kinase,
                     interferon-inducible double-stranded RNA-dependent
                     activator"
                     /codon_start=1
                     /product="interferon-inducible double-stranded
                     RNA-dependent protein kinase activator A isoform 1"
                     /protein_id="NP_003681.1"
                     /db_xref="CCDS:CCDS2279.1"
                     /db_xref="GeneID:8575"
                     /db_xref="HGNC:HGNC:9438"
                     /db_xref="MIM:603424"
                     /translation="MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGM
                     KTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKAN
                     ASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYT
                     TICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSL
                     RNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAEL
                     STSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK"
     exon            211..380
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            381..462
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            463..541
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            542..659
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            660..754
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            755..929
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1770
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1614..1619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="hexamer: ATTAAA"
     polyA_site      1640
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="major polyA site"
     regulatory      1733..1738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="hexamer: AATAAA"
     polyA_site      1770
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="major polyA site"
ORIGIN      
        1 ccctcgctgg agcaacgcaa gcaggaggcg ggggagtcgg aggaggtggc ggcgctggag
       61 ctcctcccgg ggaccagcga cccggggagc gagcacgtcg ctccgcaccg ctcttcctcc
      121 agccgctgag ccgtcccttc tcgccatgtc ccagagcagg caccgcgccg aggccccgcc
      181 gctggagcgc gaggacagtg ggaccttcag tttggggaag atgataacag ctaagccagg
      241 gaaaacaccg attcaggtat tacacgaata cggcatgaag accaagaaca tcccagttta
      301 tgaatgtgaa agatctgatg tgcaaataca cgtgcccact ttcaccttca gagtaaccgt
      361 tggtgacata acctgcacag gtgaaggtac aagtaagaag ctggcgaaac atagagctgc
      421 agaggctgcc ataaacattt tgaaagccaa tgcaagtatt tgctttgcag ttcctgaccc
      481 cttaatgcct gacccttcca agcaaccaaa gaaccagctt aatcctattg gttcattaca
      541 ggaattggct attcatcatg gctggagact tcctgaatat accctttccc aggagggagg
      601 acctgctcat aagagagaat atactacaat ttgcaggcta gagtcattta tggaaactgg
      661 aaagggggca tcaaaaaagc aagccaaaag gaatgctgct gagaaatttc ttgccaaatt
      721 tagtaatatt tctccagaga accacatttc tttaacaaat gtagtaggac attctttagg
      781 atgtacttgg cattccttga ggaattctcc tggtgaaaag atcaacttac tgaaaagaag
      841 cctccttagt attccaaata cagattacat ccagctgctt agtgaaattg ccaaggaaca
      901 aggttttaat ataacatatt tggatataga tgaactgagc gccaatggac aatatcaatg
      961 tcttgctgaa ctgtccacca gccccatcac agtctgtcat ggctccggta tctcctgtgg
     1021 caatgcacaa agtgatgcag ctcacaatgc tttgcagtat ttaaagataa tagcagaaag
     1081 aaagtaaatc tggagcaact taaaaaatct ttcagtagca cataaaaagt tcccctctgg
     1141 ccccttccca agtaaaactt ttaccgtagt gtttatgtct tgtttctaaa tctcttcata
     1201 gattccatca acactccaga tttaattatc tcctcatagt tgttattaag ctctttttaa
     1261 tggcttcaac tttgtatcag tatactgtat ttataaactt tgtaccacaa gagagagtgt
     1321 agcacccatt ttacagtgcc atgcacatca gagaaagaaa ctgcatgttt gttgttgatg
     1381 atgaaataaa aatgctagcg acagtctttc ttactggtgc ttaagctctt ctttgcacaa
     1441 agctttataa agggaattca aaggaagccc tttagaatta gagtcttgag ggacagcact
     1501 aacaggcctt tattaagtat gattgattgt taaatttcag ggaacatgat tggtctgctg
     1561 tgtatttgaa ttcatgtaac aaagaactgt tacgatggga ttctgctcat tttattaaaa
     1621 agctactgac ttgactgtca tcctgttctt gttagccatt gtgaataaga ttttaatgtt
     1681 gataattctg ttatttacat atctctaatt tactttgaaa ttcaaaggtg aaaataaaaa
     1741 atgatggcct aagtaaaatt tacaaacata
//
