LOCUS       NM_001139517            1906 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens protein activator of interferon induced protein kinase
            EIF2AK2 (PRKRA), transcript variant 2, mRNA.
ACCESSION   NM_001139517
VERSION     NM_001139517.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Shivaprasad,S., Qiao,W., Weng,K.F., Umashankar,P., Carette,J.E. and
            Sarnow,P.
  TITLE     CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral
            Replication
  JOURNAL   Viruses 16 (5), 725 (2024)
   PUBMED   38793607
  REMARK    GeneRIF: CRISPR Screen Reveals PACT as a Pro-Viral Factor for
            Dengue Viral Replication.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Lin,Y.Y., Yao,R., Zhuang,J., Wang,B., Du,L., Guo,D., Pan,J.A. and
            Peng,X.
  TITLE     PACT inhibits the replication of SARS-CoV-2 through the blockage of
            GSK-3beta-N-nsp3 cascade
  JOURNAL   J Med Virol 95 (6), e28832 (2023)
   PUBMED   37264691
  REMARK    GeneRIF: PACT inhibits the replication of SARS-CoV-2 through the
            blockage of GSK-3beta-N-nsp3 cascade.
REFERENCE   3  (bases 1 to 1906)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   4  (bases 1 to 1906)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   5  (bases 1 to 1906)
  AUTHORS   Wei,L., Wang,W., Yao,J., Cui,Z., Xu,Z., Ding,H., Wu,X., Wang,D.,
            Luo,J. and Ke,Z.J.
  TITLE     PACT promotes the metastasis of basal-like breast cancer through
            Rac1 SUMOylation and activation
  JOURNAL   Oncogene 41 (37), 4282-4294 (2022)
   PUBMED   35974143
  REMARK    GeneRIF: PACT promotes the metastasis of basal-like breast cancer
            through Rac1 SUMOylation and activation.
REFERENCE   6  (bases 1 to 1906)
  AUTHORS   Horng,T., Barton,G.M. and Medzhitov,R.
  TITLE     TIRAP: an adapter molecule in the Toll signaling pathway
  JOURNAL   Nat Immunol 2 (9), 835-841 (2001)
   PUBMED   11526399
REFERENCE   7  (bases 1 to 1906)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 1906)
  AUTHORS   Ito,T., Yang,M. and May,W.S.
  TITLE     RAX, a cellular activator for double-stranded RNA-dependent protein
            kinase during stress signaling
  JOURNAL   J Biol Chem 274 (22), 15427-15432 (1999)
   PUBMED   10336432
REFERENCE   9  (bases 1 to 1906)
  AUTHORS   Patel,R.C. and Sen,G.C.
  TITLE     PACT, a protein activator of the interferon-induced protein kinase,
            PKR
  JOURNAL   EMBO J 17 (15), 4379-4390 (1998)
   PUBMED   9687506
REFERENCE   10 (bases 1 to 1906)
  AUTHORS   Simons,A., Melamed-Bessudo,C., Wolkowicz,R., Sperling,J.,
            Sperling,R., Eisenbach,L. and Rotter,V.
  TITLE     PACT: cloning and characterization of a cellular p53 binding
            protein that interacts with Rb
  JOURNAL   Oncogene 14 (2), 145-155 (1997)
   PUBMED   9010216
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL833867.1 and AC009948.3.
            
            Summary: This gene encodes a protein kinase activated by
            double-stranded RNA which mediates the effects of interferon in
            response to viral infection. Mutations in this gene have been
            associated with dystonia. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Nov 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. This difference causes translation
            initiation at a downstream AUG and an isoform (2) with a shorter
            N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL833867.1, SRR1660807.14901.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1898              AL833867.1         1-1898
            1899-1906           AC009948.3         38004-38011         c
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..1906
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="protein activator of interferon induced protein
                     kinase EIF2AK2"
                     /db_xref="GeneID:8575"
                     /db_xref="HGNC:HGNC:9438"
                     /db_xref="MIM:603424"
     exon            1..516
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..1223
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein activator of the interferon-induced protein
                     kinase; PKR-associated protein X; PKR-associating protein
                     X; interferon-inducible double-stranded RNA-dependent
                     protein kinase activator A; protein kinase,
                     interferon-inducible double-stranded RNA-dependent
                     activator"
                     /codon_start=1
                     /product="interferon-inducible double-stranded
                     RNA-dependent protein kinase activator A isoform 2"
                     /protein_id="NP_001132989.1"
                     /db_xref="CCDS:CCDS46460.1"
                     /db_xref="GeneID:8575"
                     /db_xref="HGNC:HGNC:9438"
                     /db_xref="MIM:603424"
                     /translation="MQSTPFCGFCSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECE
                     RSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPL
                     MPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMET
                     GKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLL
                     KRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGS
                     GISCGNAQSDAAHNALQYLKIIAERK"
     exon            517..598
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            599..677
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            678..795
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            796..890
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1065
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1906
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1750..1755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="hexamer: ATTAAA"
     polyA_site      1776
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="major polyA site"
     regulatory      1869..1874
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="hexamer: AATAAA"
     polyA_site      1906
                     /gene="PRKRA"
                     /gene_synonym="DYT16; HSD14; PACT; RAX"
                     /note="major polyA site"
ORIGIN      
        1 gcggggcggg cggaggtgac gctcggtcgg cgcgcccggg aagggatcgt caggttttcc
       61 ctgagaggct gcggcgctgc tgccagccct gttctgttga gaatctctcc tacccgcatt
      121 caagtgctct ctctaaagac ctcgctcacc ccgcctgctc ctgcggagtg actgccgcag
      181 cggggccgga agacaggtgc gccccagcct cgccagctct ggccctggcc tccggggaac
      241 tcggcgcctc cctcgtcacc gtcggtggcg gttaaaactg gcctctacgc ttctgcttca
      301 atctccaatt tggcatgcaa agcacacctt tttgtggttt ttgcagtttg gggaagatga
      361 taacagctaa gccagggaaa acaccgattc aggtattaca cgaatacggc atgaagacca
      421 agaacatccc agtttatgaa tgtgaaagat ctgatgtgca aatacacgtg cccactttca
      481 ccttcagagt aaccgttggt gacataacct gcacaggtga aggtacaagt aagaagctgg
      541 cgaaacatag agctgcagag gctgccataa acattttgaa agccaatgca agtatttgct
      601 ttgcagttcc tgacccctta atgcctgacc cttccaagca accaaagaac cagcttaatc
      661 ctattggttc attacaggaa ttggctattc atcatggctg gagacttcct gaatataccc
      721 tttcccagga gggaggacct gctcataaga gagaatatac tacaatttgc aggctagagt
      781 catttatgga aactggaaag ggggcatcaa aaaagcaagc caaaaggaat gctgctgaga
      841 aatttcttgc caaatttagt aatatttctc cagagaacca catttcttta acaaatgtag
      901 taggacattc tttaggatgt acttggcatt ccttgaggaa ttctcctggt gaaaagatca
      961 acttactgaa aagaagcctc cttagtattc caaatacaga ttacatccag ctgcttagtg
     1021 aaattgccaa ggaacaaggt tttaatataa catatttgga tatagatgaa ctgagcgcca
     1081 atggacaata tcaatgtctt gctgaactgt ccaccagccc catcacagtc tgtcatggct
     1141 ccggtatctc ctgtggcaat gcacaaagtg atgcagctca caatgctttg cagtatttaa
     1201 agataatagc agaaagaaag taaatctgga gcaacttaaa aaatctttca gtagcacata
     1261 aaaagttccc ctctggcccc ttcccaagta aaacttttac cgtagtgttt atgtcttgtt
     1321 tctaaatctc ttcatagatt ccatcaacac tccagattta attatctcct catagttgtt
     1381 attaagctct ttttaatggc ttcaactttg tatcagtata ctgtatttat aaactttgta
     1441 ccacaagaga gagtgtagca cccattttac agtgccatgc acatcagaga aagaaactgc
     1501 atgtttgttg ttgatgatga aataaaaatg ctagcgacag tctttcttac tggtgcttaa
     1561 gctcttcttt gcacaaagct ttataaaggg aattcaaagg aagcccttta gaattagagt
     1621 cttgagggac agcactaaca ggcctttatt aagtatgatt gattgttaaa tttcagggaa
     1681 catgattggt ctgctgtgta tttgaattca tgtaacaaag aactgttacg atgggattct
     1741 gctcatttta ttaaaaagct actgacttga ctgtcatcct gttcttgtta gccattgtga
     1801 ataagatttt aatgttgata attctgttat ttacatatct ctaatttact ttgaaattca
     1861 aaggtgaaaa taaaaaatga tggcctaagt aaaatttaca aacata
//
