LOCUS       NM_006258               6615 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript
            variant 2, mRNA.
ACCESSION   NM_006258
VERSION     NM_006258.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Li,Z., Chen,Z., Liu,C., Peng,S. and Wang,N.
  TITLE     PKG1 promotes the HIV-induced proliferation, migration, and
            fibrosis of vascular smooth muscle cells of hemorrhoids
  JOURNAL   Int J Colorectal Dis 39 (1), 175 (2024)
   PUBMED   39477875
  REMARK    GeneRIF: PKG1 promotes the HIV-induced proliferation, migration,
            and fibrosis of vascular smooth muscle cells of hemorrhoids.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Liu,J., Wei,B., Zhang,Y., You,Y. and Zhi,Y.
  TITLE     Association between PRKG1 gene and gene-environment interactions
            with pediatric asthma
  JOURNAL   J Asthma 61 (7), 754-761 (2024)
   PUBMED   38193459
  REMARK    GeneRIF: Association between PRKG1 gene and gene-environment
            interactions with pediatric asthma.
REFERENCE   3  (bases 1 to 6615)
  AUTHORS   Yang,X., Liu,Q. and Li,G.
  TITLE     Anti-NSCLC role of SCN4B by negative regulation of the cGMP-PKG
            pathway: Integrated utilization of bioinformatics analysis and in
            vitro assay validation
  JOURNAL   Drug Dev Res 85 (3), e22192 (2024)
   PUBMED   38678552
  REMARK    GeneRIF: Anti-NSCLC role of SCN4B by negative regulation of the
            cGMP-PKG pathway: Integrated utilization of bioinformatics analysis
            and in vitro assay validation.
REFERENCE   4  (bases 1 to 6615)
  AUTHORS   Lan,T., Li,Y., Wang,Y., Wang,Z.C., Mu,C.Y., Tao,A.B., Gong,J.L.,
            Zhou,Y., Xu,H., Li,S.B., Gu,B., Ma,P. and Luo,L.
  TITLE     Increased endogenous PKG I activity attenuates EGF-induced
            proliferation and migration of epithelial ovarian cancer via the
            MAPK/ERK pathway
  JOURNAL   Cell Death Dis 14 (1), 39 (2023)
   PUBMED   36653376
  REMARK    GeneRIF: Increased endogenous PKG I activity attenuates EGF-induced
            proliferation and migration of epithelial ovarian cancer via the
            MAPK/ERK pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6615)
  AUTHORS   Micale,L., Fusco,C., Nardella,G., Palmieri,O., Latiano,T.,
            Gioffreda,D., Tavano,F., Panza,A., Merla,A., Biscaglia,G.,
            Gentile,M., Cuttitta,A., Castori,M., Perri,F. and Latiano,A.
  TITLE     Downexpression of miR-200c-3p Contributes to Achalasia Disease by
            Targeting the PRKG1 Gene
  JOURNAL   Int J Mol Sci 24 (1), 668 (2022)
   PUBMED   36614110
  REMARK    GeneRIF: Downexpression of miR-200c-3p Contributes to Achalasia
            Disease by Targeting the PRKG1 Gene.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6615)
  AUTHORS   Pohler,D., Butt,E., Meissner,J., Muller,S., Lohse,M., Walter,U.,
            Lohmann,S.M. and Jarchau,T.
  TITLE     Expression, purification, and characterization of the
            cGMP-dependent protein kinases I beta and II using the baculovirus
            system
  JOURNAL   FEBS Lett 374 (3), 419-425 (1995)
   PUBMED   7589584
REFERENCE   7  (bases 1 to 6615)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
REFERENCE   8  (bases 1 to 6615)
  AUTHORS   Picciotto,M.R., Cohn,J.A., Bertuzzi,G., Greengard,P. and Nairn,A.C.
  TITLE     Phosphorylation of the cystic fibrosis transmembrane conductance
            regulator
  JOURNAL   J Biol Chem 267 (18), 12742-12752 (1992)
   PUBMED   1377674
REFERENCE   9  (bases 1 to 6615)
  AUTHORS   Orstavik,S., Sandberg,M., Berube,D., Natarajan,V., Simard,J.,
            Walter,U., Gagne,R., Hansson,V. and Jahnsen,T.
  TITLE     Localization of the human gene for the type I cyclic GMP-dependent
            protein kinase to chromosome 10
  JOURNAL   Cytogenet Cell Genet 59 (4), 270-273 (1992)
   PUBMED   1544322
REFERENCE   10 (bases 1 to 6615)
  AUTHORS   Sandberg,M., Natarajan,V., Ronander,I., Kalderon,D., Walter,U.,
            Lohmann,S.M. and Jahnsen,T.
  TITLE     Molecular cloning and predicted full-length amino acid sequence of
            the type I beta isozyme of cGMP-dependent protein kinase from human
            placenta. Tissue distribution and developmental changes in rat
  JOURNAL   FEBS Lett 255 (2), 321-329 (1989)
   PUBMED   2792381
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA150150.1, BC127090.1,
            AL599933.1, BX952280.1, DB138350.1, DA915933.1, DA646702.1,
            CD723759.1, BX097700.1 and BQ445850.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2018 this sequence version replaced NM_006258.3.
            
            Summary: Mammals have three different isoforms of cyclic
            GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG
            isoforms act as key mediators of the nitric oxide/cGMP signaling
            pathway and are important components of many signal transduction
            processes in diverse cell types. This PRKG1 gene on human
            chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG
            by alternative transcript splicing. A separate gene on human
            chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II.
            The PRKG1 proteins play a central role in regulating cardiovascular
            and neuronal functions in addition to relaxing smooth muscle tone,
            preventing platelet aggregation, and modulating cell growth. This
            gene is most strongly expressed in all types of smooth muscle,
            platelets, cerebellar Purkinje cells, hippocampal neurons, and the
            lateral amygdala. Isoforms Ialpha and Ibeta have identical
            cGMP-binding and catalytic domains but differ in their
            leucine/isoleucine zipper and autoinhibitory sequences and
            therefore differ in their dimerization substrates and kinase enzyme
            activity. [provided by RefSeq, Sep 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.196058.1,
                                           SRR1803614.29052.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373980.11/ ENSP00000363092.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                DA150150.1         14-59
            47-3092             BC127090.1         1-3046
            3093-3401           AL599933.1         240-548
            3402-3948           BX952280.1         19-565
            3949-4472           DB138350.1         15-538
            4473-4873           DA915933.1         121-521
            4874-5430           DA646702.1         79-635
            5431-5679           CD723759.1         370-618
            5680-5923           BX097700.1         186-429
            5924-6615           BQ445850.1         17-708              c
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23-q21.1"
     gene            1..6615
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /note="protein kinase cGMP-dependent 1"
                     /db_xref="GeneID:5592"
                     /db_xref="HGNC:HGNC:9414"
                     /db_xref="MIM:176894"
     exon            1..415
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2165
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /EC_number="2.7.11.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     cGMP-dependent protein kinase 1; protein kinase,
                     cGMP-dependent, regulatory, type I, beta; protein kinase,
                     cGMP-dependent, type I"
                     /codon_start=1
                     /product="cGMP-dependent protein kinase 1 isoform 2"
                     /protein_id="NP_006249.1"
                     /db_xref="CCDS:CCDS7244.1"
                     /db_xref="GeneID:5592"
                     /db_xref="HGNC:HGNC:9414"
                     /db_xref="MIM:176894"
                     /translation="MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQN
                     ELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKS
                     PQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMED
                     GKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMR
                     TGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFII
                     SKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRD
                     SFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELV
                     QLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLY
                     MLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILD
                     HRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYEL
                     LTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNG
                     VKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGW
                     DIDF"
     exon            416..582
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            583..696
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            697..802
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            803..866
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            867..944
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1039
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1105
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1180
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1277
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1417
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1507
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1649
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1813
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1936
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..1999
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2066
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..6615
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6592..6597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /note="hexamer: AATAAA"
     polyA_site      6615
                     /gene="PRKG1"
                     /gene_synonym="AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha;
                     cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B"
                     /note="major polyA site"
ORIGIN      
        1 actcgggagg cagcggcgac tttggggaaa gttgatcgga gaggggagga agcctcaaga
       61 cgcggagcag cggcaggaag gagcccccgg cagcccggag gagcatgggc accttgcggg
      121 atttacagta cgcgctccag gagaagatcg aggagctgag gcagcgggat gctctcatcg
      181 acgagctgga gctggagttg gatcagaagg acgaactgat ccagaagctg cagaacgagc
      241 tggacaagta ccgctcggtg atccgaccag ccacccagca ggcgcagaag cagagcgcga
      301 gcaccttgca gggcgagccg cgcaccaagc ggcaggcgat ctccgccgag cccaccgcct
      361 tcgacatcca ggatctcagc catgtgaccc tgcccttcta ccccaagagc ccacagtcca
      421 aggatcttat aaaggaagct atccttgaca atgactttat gaagaacttg gagctgtcgc
      481 agatccagga gattgtggat tgtatgtacc cggtggagta tggcaaggac agttgcatca
      541 tcaaagaagg agacgtgggg tcactggtgt atgtcatgga agatggtaag gttgaagtta
      601 caaaagaagg tgtgaagttg tgtaccatgg gtccaggaaa agtgtttggg gaattggcta
      661 ttctttacaa ctgtacccgg acagcgaccg tcaagactct tgtaaatgta aaactctggg
      721 ccattgatcg acaatgtttt caaacaataa tgatgaggac aggactcatc aagcataccg
      781 agtatatgga atttttaaaa agcgttccaa cattccagag ccttcctgaa gagatcctca
      841 gcaagcttgc tgatgtcctt gaagagaccc actatgaaaa tggagaatat attatcaggc
      901 aaggtgcaag aggggacacc ttctttatca tcagcaaagg aacggtaaat gtcactcgtg
      961 aagactcacc gagtgaagac ccagtctttc ttagaacttt aggaaaagga gactggtttg
     1021 gagagaaagc cttgcagggg gaagatgtga gaacagcaaa cgtaattgct gcagaagctg
     1081 taacctgcct tgtgattgac agagactctt ttaaacattt gattggaggg ctggatgatg
     1141 tttctaataa agcatatgaa gatgcagaag ctaaagcaaa atatgaagct gaagcggctt
     1201 tcttcgccaa cctgaagctg tctgatttca acatcattga tacccttgga gttggaggtt
     1261 tcggacgagt agaactggtc cagttgaaaa gtgaagaatc caaaacgttt gcaatgaaga
     1321 ttctcaagaa acgtcacatt gtggacacaa gacagcagga gcacatccgc tcagagaagc
     1381 agatcatgca gggggctcat tccgatttca tagtgagact gtacagaaca tttaaggaca
     1441 gcaaatattt gtatatgttg atggaagctt gtctaggtgg agagctctgg accattctca
     1501 gggatagagg ttcgtttgaa gattctacaa ccagatttta cacagcatgt gtggtagaag
     1561 cttttgccta tctgcattcc aaaggaatca tttacaggga cctcaagcca gaaaatctca
     1621 tcctagatca ccgaggttat gccaaactgg ttgattttgg ctttgcaaag aaaataggat
     1681 ttggaaagaa aacatggact ttttgtggga ctccagagta tgtagcccca gagatcatcc
     1741 tgaacaaagg ccatgacatt tcagccgact actggtcact gggaatccta atgtatgaac
     1801 tcctgactgg cagcccacct ttctcaggcc cagatcctat gaaaacctat aacatcatat
     1861 tgagggggat tgacatgata gaatttccaa agaagattgc caaaaatgct gctaatttaa
     1921 ttaaaaaact atgcagggac aatccatcag aaagattagg gaatttgaaa aatggagtaa
     1981 aagacattca aaagcacaaa tggtttgagg gctttaactg ggaaggctta agaaaaggta
     2041 ccttgacacc tcctataata ccaagtgttg catcacccac agacacaagt aattttgaca
     2101 gtttccctga ggacaacgat gaaccaccac ctgatgacaa ctcaggatgg gatatagact
     2161 tctaatgtat ttctcttacc tgcttctgcc ttgctgaaga cagctttttc tgagacacag
     2221 ctgccagcaa acctgaggga aagagagaag attagtgctc ggggtcacca tgatgccttt
     2281 gatcgatgct gctccagtaa ctacagtggc attaggactt accgcttaga tgacaatagt
     2341 gctctttaca tgttttctgt ttgaacctaa aatagcagtt gacatggtgg tcctgaagca
     2401 aagcctttca ccagtaaaag atgttttcta ttgttgcaat gaccttgctt tgctctgatt
     2461 ataatttgaa agactgtagg aaacacttca atgtagtata agagtctgta ccttgctgga
     2521 atattcaaga agatgaaaga ataatatatt gggtacaata gattactatg gtacagaaac
     2581 tgggctattc cctttcttca agtgaaggct gtgggatcta ttactgcagg ccggtgtata
     2641 taccatacaa aagaggacca cacatctgtt ggtcacagag ttcatgtcac accagtgcta
     2701 gaagtttcat gattttattt cccagcagtg ctgatgacaa gactgaatgt taccttttct
     2761 ttctgacaga ttttaaaaat tgatatgata aaagcacaac tgctatagat tctgctgaga
     2821 cctctcatag taggtatata tgagttttca cagaagactg aaaaataatg catgatattt
     2881 gtttgttttt tttgataaat tggcatgaca gagtggggaa aaaaagcaat tcacaaaacc
     2941 atttcatatt ttttaaaata ttgtgcttaa agatggtcct ggaagtaaat gactagcagc
     3001 caattggttt tacttaacat accctcaaac tgaggcttaa agtattccct tttataaaaa
     3061 taaatgcttg gggtagggtg gagtggggag ggattaaaac ccatccaaaa aataaataaa
     3121 aactatatag gtgctatgta tatctttcat ctgtaaatgt cagtgtctga acagacaaca
     3181 caaattcaaa tcattatacg tgtagccaga aactcaagca ttttcactaa agttattaaa
     3241 ccaaactcct gtccaatttc acttatacaa catagtcagt ctagagttga gagacaaagg
     3301 taattataaa cctatttgaa ctagcttctt gtcttaggcc tgaaccaaaa aacaacaaac
     3361 aaacaaaaaa caagaatgaa aaacagaaat aaaagaagta gaaaagacaa agaaagaaag
     3421 cccaaagtca aagttgttaa tatttacagg tttaccagat ctggaacatt acttatttga
     3481 ggtcagagaa caaaacaaga acctggccag gtgttgatta ccttttagtg aataagctga
     3541 gtccatatac ttgtctaact aagaaagcag tacagaggaa aacaggaacc tgattttttt
     3601 aaaataaatt ttaaataaaa tagaattact acaattctgc aatttcatac tacctaaaaa
     3661 agactagatt tgaaaatgtc aagctgattt actttattca catggagaaa agaatccaca
     3721 aattaaactg agtccttcac tggcatgcca gttgactatt attagctgtc ataagtaact
     3781 cagccatttg gaagcatttc ctggacaaaa ttacattata cctgtgaaga aaaatataga
     3841 agctttcaag ctgtcattct gttttggccc tgtgtgaatt tggtatatta aattgacgct
     3901 tgcttttttt ttcagtagag ttttcttttt aatcaatcaa acgcctacat ttctggaaga
     3961 aaaagtccaa ttagatcatt acaccttaac taagaaggca aatcttataa attttgcttg
     4021 aattgttctt actcaaaact accttagaat atggtatctt atgaaaatat aatattctta
     4081 aaattttgga ggataaaatg aaaagggtat aaacttcaat tagaacttta atcctaaaat
     4141 attgttattc ttgtattttt ggagttttcc gtcccttaca atttctccaa attggtctat
     4201 tccccaagat atccactgtt ttctacagat tttagaatat aaaattaaac atatcaacct
     4261 aaaatctagc tatgaatgtt atttcaagag ttttatattt ttaattggct ttttaaaaat
     4321 atgtaattcc aatattatag tgaatgttca gtaacttttg ttgaaagata atgtggttta
     4381 ggcttgctaa agaaaggctt gctagaatac taaatttacc ttgttatttg gaaagagaat
     4441 ctgctcctat aacgttttaa aaatccccaa agtcccagag attagcactt gctatggatt
     4501 ttggaactat ggttcaagtc taagacaacc aatatgaata agttcaatgg agaaggaaga
     4561 agtagcattc agcacaagaa actgtagatt gtttctccca cttgttactg catctttgtt
     4621 catttcaccc atagcattgc atttgatgtc tgaagtaatt accacaaaat aaaggtaaac
     4681 tacatcttcc aagatgtacc aacagaaaac attcgtctaa ttttgtcaac attgaatttt
     4741 taggatttaa atcttgtctt aatgaagact ctagggctaa aattcatggt cagttccata
     4801 aaatgcatct cactttattt ctgaattttt cacaacccct ctattttctt acattaaaga
     4861 acatctggat aagttagaat agtgccaact tgtttgattt gttatatgat tttcttgtta
     4921 atgtgttgag tatgggaaat aagggctttc tgtgtgtctt gactcacagg aaaactgaaa
     4981 ctgccaaggg aagcaacatt aaaacaactg ggatagattg aatacatttg aaaaaataat
     5041 ttctggaaaa acgctataca tgctttttat gtttatatct tctttcttat taagacatga
     5101 atcagatcca tgtggcattc aaaacaaatt tattttggtt atattcttta caagtcataa
     5161 tttttaccta aaattgtgga atatgcatgt gaattacaca tgtgtagtaa taaactcaac
     5221 tattgaattt ttcctgtttc atttctaaat gactgaactg atggtaaaca aatttaatga
     5281 agtcagctac catgttgaaa ataaggatat tagaactgat ttcatacaat agaggtgatc
     5341 tgtgcattat ccataacaac gttttctttc actataggac cacagacaaa tatttatgta
     5401 aataggtatt tttgtgtgat attttgtggt acatataact ttttttagtg tttcacagca
     5461 ttattatttc attttatttg tattgaataa tgtttttagg tccaagtaga cttgaattaa
     5521 tgtcataatt gtcagtatta ttgaaaatta tgtcaactgt actgttattc atctgtccca
     5581 tagtttagaa catgacctgg ctatctggca ttgttgcaag tgccttaaat tcatggcatc
     5641 ctattaaaaa aacaaatttg gtgttatgct gcatgacaaa gacaaacaca cctcaaaatg
     5701 ttgtcctttc ttagcttgct gcgttttaca gttctttctg ctaacaattt ataagccttt
     5761 attatataat attgatgaat tacagaaatg atgaagttgt tctcttattt ctgttaaatg
     5821 taccagagct gtgtatctgt taatgagata cagcgtcatt tctgtgaaat gtataaatgt
     5881 atgtgcaggt caaattaata tatgacattc tatcagtctg tatacaggta ttcctgtttt
     5941 gctaagctgt gcagattctg taaaaataaa ttagtgcagt caagttctaa catatctcat
     6001 tttatagact ccattgacaa tggtccaacc gaaaagaaaa attggaaatg actgctaacc
     6061 agacctcgtt atgaaaccaa tgatgaaaaa gtcacttggt gcacctctca acaaaacagg
     6121 atgctttttc cgagttcttg tatatgcaaa tcatttacga ggcaagtttt caacagacct
     6181 agaaagcaaa acattgaaag tgtgttgtta gccctcctct ctgcccctga atcaacagac
     6241 agagctctgg gaccttggca atgactcaga ggttggggct ttctgagtaa agggacattt
     6301 ctttaagcat catctatcaa atttatttca atgtatgatg tttttacaac tggtcagttc
     6361 ttttgtattc ttacagctat ggttatttga ttgtcctctt acaatttgtt ctacatgaaa
     6421 gagttctttg ttagtcagaa tgcaacaaac tgtcatcaaa tggtcattga ccacttgcac
     6481 tcttttgatg tagattaaaa actttttcat aaatttacct gctttgattt gctctgtatt
     6541 tttcctgcag ctgtaattgc tgagtgcctg ttgtatactt tatagagttg aaataaaaaa
     6601 ataattactt ttgaa
//
