LOCUS       NM_016457               3588 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens protein kinase D2 (PRKD2), transcript variant 1, mRNA.
ACCESSION   NM_016457
VERSION     NM_016457.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Liu,Y.Q., Xu,Y.W., Zheng,Z.T., Li,D., Hong,C.Q., Dai,H.Q.,
            Wang,J.H., Chu,L.Y., Liao,L.D., Zou,H.Y., Li,E.M., Xie,J.J. and
            Fang,W.K.
  TITLE     Serine/threonine-protein kinase D2-mediated phosphorylation of DSG2
            threonine 730 promotes esophageal squamous cell carcinoma
            progression
  JOURNAL   J Pathol 263 (1), 99-112 (2024)
   PUBMED   38411280
  REMARK    GeneRIF: Serine/threonine-protein kinase D2-mediated
            phosphorylation of DSG2 threonine 730 promotes esophageal squamous
            cell carcinoma progression.
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Obata,Y., Kurokawa,K., Tojima,T., Natsume,M., Shiina,I.,
            Takahashi,T., Abe,R., Nakano,A. and Nishida,T.
  TITLE     Golgi retention and oncogenic KIT signaling via
            PLCgamma2-PKD2-PI4KIIIbeta activation in gastrointestinal stromal
            tumor cells
  JOURNAL   Cell Rep 42 (9), 113035 (2023)
   PUBMED   37616163
  REMARK    GeneRIF: Golgi retention and oncogenic KIT signaling via
            PLCgamma2-PKD2-PI4KIIIbeta activation in gastrointestinal stromal
            tumor cells.
REFERENCE   3  (bases 1 to 3588)
  AUTHORS   Feng,R., Wang,X., Chen,H., Cao,C., Liu,T., Zhao,T., Chen,H.,
            Tian,R., Ni,Y., Tian,X., Hu,Z., Ma,J. and Gong,D.
  TITLE     Knockdown of PRKD2 Enhances Chemotherapy Sensitivity in Cervical
            Cancer via the TP53/CDKN1A Pathway
  JOURNAL   Curr Cancer Drug Targets 23 (2), 159-170 (2023)
   PUBMED   36017858
  REMARK    GeneRIF: Knockdown of PRKD2 Enhances Chemotherapy Sensitivity in
            Cervical Cancer via the TP53/CDKN1A Pathway.
REFERENCE   4  (bases 1 to 3588)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3588)
  AUTHORS   Trujillo-Viera,J., El-Merahbi,R., Schmidt,V., Karwen,T.,
            Loza-Valdes,A., Strohmeyer,A., Reuter,S., Noh,M., Wit,M., Hawro,I.,
            Mocek,S., Fey,C., Mayer,A.E., Loffler,M.C., Wilhelmi,I.,
            Metzger,M., Ishikawa,E., Yamasaki,S., Rau,M., Geier,A., Hankir,M.,
            Seyfried,F., Klingenspor,M. and Sumara,G.
  TITLE     Protein Kinase D2 drives chylomicron-mediated lipid transport in
            the intestine and promotes obesity
  JOURNAL   EMBO Mol Med 13 (5), e13548 (2021)
   PUBMED   33949105
  REMARK    GeneRIF: Protein Kinase D2 drives chylomicron-mediated lipid
            transport in the intestine and promotes obesity.
REFERENCE   6  (bases 1 to 3588)
  AUTHORS   Mihailovic,T., Marx,M., Auer,A., Van Lint,J., Schmid,M., Weber,C.
            and Seufferlein,T.
  TITLE     Protein kinase D2 mediates activation of nuclear factor kappaB by
            Bcr-Abl in Bcr-Abl+ human myeloid leukemia cells
  JOURNAL   Cancer Res 64 (24), 8939-8944 (2004)
   PUBMED   15604256
  REMARK    GeneRIF: Data show that Bcr-Abl-induced activation of the nuclear
            factor kappaB cascade in myeloid leukemia cells is largely mediated
            by tyrosine-phosphorylated PKD2.
REFERENCE   7  (bases 1 to 3588)
  AUTHORS   Yeaman,C., Ayala,M.I., Wright,J.R., Bard,F., Bossard,C., Ang,A.,
            Maeda,Y., Seufferlein,T., Mellman,I., Nelson,W.J. and Malhotra,V.
  TITLE     Protein kinase D regulates basolateral membrane protein exit from
            trans-Golgi network
  JOURNAL   Nat Cell Biol 6 (2), 106-112 (2004)
   PUBMED   14743217
  REMARK    GeneRIF: specificity of different PKD isoforms in regulating
            protein trafficking from the trans-Golgi network
REFERENCE   8  (bases 1 to 3588)
  AUTHORS   Rey,O., Yuan,J. and Rozengurt,E.
  TITLE     Intracellular redistribution of protein kinase D2 in response to
            G-protein-coupled receptor agonists
  JOURNAL   Biochem Biophys Res Commun 302 (4), 817-824 (2003)
   PUBMED   12646243
REFERENCE   9  (bases 1 to 3588)
  AUTHORS   Sturany,S., Van Lint,J., Gilchrist,A., Vandenheede,J.R., Adler,G.
            and Seufferlein,T.
  TITLE     Mechanism of activation of protein kinase D2(PKD2) by the
            CCK(B)/gastrin receptor
  JOURNAL   J Biol Chem 277 (33), 29431-29436 (2002)
   PUBMED   12058027
  REMARK    GeneRIF: mechanism of activation by CCK(B)/gastrin receptor
REFERENCE   10 (bases 1 to 3588)
  AUTHORS   Sturany,S., Van Lint,J., Muller,F., Wilda,M., Hameister,H.,
            Hocker,M., Brey,A., Gern,U., Vandenheede,J., Gress,T., Adler,G. and
            Seufferlein,T.
  TITLE     Molecular cloning and characterization of the human protein kinase
            D2. A novel member of the protein kinase D family of serine
            threonine kinases
  JOURNAL   J Biol Chem 276 (5), 3310-3318 (2001)
   PUBMED   11062248
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008635.6, AF309082.1 and
            AL050147.1.
            
            On May 21, 2020 this sequence version replaced NM_016457.4.
            
            Summary: The protein encoded by this gene belongs to the protein
            kinase D (PKD) family of serine/threonine protein kinases. This
            kinase can be activated by phorbol esters as well as by gastrin via
            the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It
            can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN)
            and may regulate basolateral membrane protein exit from TGN.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, and 3 encode the same isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF309082.1, AK092896.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291281.9/ ENSP00000291281.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-705               AC008635.6         20410-21114
            706-2447            AF309082.1         1-1742
            2448-3588           AL050147.1         698-1838
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..3588
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /note="protein kinase D2"
                     /db_xref="GeneID:25865"
                     /db_xref="HGNC:HGNC:17293"
                     /db_xref="MIM:607074"
     exon            1..984
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             745..3381
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /EC_number="2.7.11.13"
                     /note="isoform A is encoded by transcript variant 1;
                     serine/threonine-protein kinase D2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase D2 isoform A"
                     /protein_id="NP_057541.2"
                     /db_xref="CCDS:CCDS12689.1"
                     /db_xref="GeneID:25865"
                     /db_xref="HGNC:HGNC:17293"
                     /db_xref="MIM:607074"
                     /translation="MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSG
                     VSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTS
                     ANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLF
                     GLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCT
                     AEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPT
                     VCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSE
                     ADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTT
                     RKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVE
                     SAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQAL
                     MPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVY
                     GGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVF
                     VVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLL
                     ASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVI
                     MYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSV
                     DKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRD
                     LGGACPPQDHDMQGLAERISVL"
     exon            985..1123
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1255
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1410
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1633
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1711
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1865
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1983
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2061
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2320
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2373
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2446
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2553
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2554..2715
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2814
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..3082
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3083..3168
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3588
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3545..3550
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /note="hexamer: ATTAAA"
     polyA_site      3588
                     /gene="PRKD2"
                     /gene_synonym="HSPC187; nPKC-D2; PKD2"
                     /note="major polyA site"
ORIGIN      
        1 acacttcctc ctgggccgcc gccgccgccg ccgacttaaa ctttggaggg ggaaaaagag
       61 ctactggcgc ctggcgaccc tccctgcccc ccacccaacc ccgctccggc aacgccccct
      121 tcctcacggc tcccgaccga acttttctcc aacttctgcg actcgtgaga ttcccttcta
      181 cccactccgg ccctcgggac ccctctgccc atcccctggc cggtcgggtc cctgcgaacc
      241 cctttatctc tggaatccac tcggtccccg actcagagac tcctgccctc cacccccaag
      301 gtgaattccc ccgggccgcc ttctgagtgg gatcctcttc ttggagcact ggatcctggg
      361 attccctctg cccccttctc aatccctcct ctagggaagg ggcctttgaa tcgcgggctc
      421 tcctgatccc tgtgaccccg acctactaga tttcctctca ggcttcttgg aatctcaatc
      481 gctgggacct ccaacccact acttttctcc tttctgatct tctgggagcc ctggattccg
      541 ggcctctgac ccactatagt gcctttctct ccttcccagg accccgccat cctcaggtcc
      601 cctccgcctg ccagatcttt tctcggatcc ccgctctccc accacctgct cacgagatcc
      661 cgcggatcta gaacccaggg tcccccgggg ccccccggcc gggtcccggg tgggctccag
      721 gcggccggtc cccggcctcc ccccatggcc accgccccct cttatcccgc cgggctccct
      781 ggctctcccg ggccggggtc tcctccgccc cccggcggcc tagagctgca gtcgccgcca
      841 ccgctactgc cccagatccc ggccccgggt tccggggtct cctttcacat ccagatcggg
      901 ctgacccgcg agttcgtgct gttgcccgcc gcctccgagc tggctcatgt gaagcagctg
      961 gcctgttcca tcgtggacca gaagttccct gagtgtggct tctacggcct ttacgacaag
     1021 atcctgcttt tcaaacatga ccccacgtcg gccaacctcc tgcagctggt gcgctcgtcc
     1081 ggagacatcc aggagggcga cctggtggag gtggtgctgt cggcctcggc caccttcgag
     1141 gacttccaga tccgcccgca cgccctcacg gtgcactcct atcgggcgcc tgccttctgt
     1201 gatcactgcg gggagatgct cttcggccta gtgcgccagg gcctcaagtg cgatggctgc
     1261 gggctgaact accacaagcg ctgtgccttc agcatcccca acaactgtag tggggcccgc
     1321 aaacggcgcc tgtcatccac gtctctggcc agtggccact cggtgcgcct cggcacctcc
     1381 gagtccctgc cctgcacggc tgaagagctg agccgtagca ccaccgaact cctgcctcgc
     1441 cgtcccccgt catcctcttc ctcctcttct gcctcatcgt atacgggccg ccccattgag
     1501 ctggacaaga tgctgctctc caaggtcaag gtgccgcaca ccttcctcat ccacagctat
     1561 acacggccca ccgtttgcca ggcttgcaag aaactcctca agggcctctt ccggcagggc
     1621 ctgcaatgca aagactgcaa gtttaactgt cacaaacgct gcgccacccg cgtccctaat
     1681 gactgcctgg gggaggccct tatcaatgga gatgtgccga tggaggaggc caccgatttc
     1741 agcgaggctg acaagagcgc cctcatggat gagtcagagg actccggtgt catccctggc
     1801 tcccactcag agaatgcgct ccacgccagt gaggaggagg aaggcgaggg aggcaaggcc
     1861 cagagctccc tggggtacat ccccctaatg agggtggtgc aatcggtgcg acacacgacg
     1921 cggaaatcca gcaccacgct gcgggagggt tgggtggttc attacagcaa caaggacacg
     1981 ctgagaaagc ggcactattg gcgcctggac tgcaagtgta tcacgctctt ccagaacaac
     2041 acgaccaaca gatactataa ggaaattccg ctgtcagaaa tcctcacggt ggagtccgcc
     2101 cagaacttca gccttgtgcc gccgggcacc aacccacact gctttgagat cgtcactgcc
     2161 aatgccacct acttcgtggg cgagatgcct ggcgggactc cgggtgggcc aagtgggcag
     2221 ggggctgagg ccgcccgggg ctgggagaca gccatccgcc aggccctgat gcccgtcatc
     2281 cttcaggacg cacccagcgc cccaggccac gcgccccaca gacaagcttc tctgagcatc
     2341 tctgtgtcca acagtcagat ccaagagaat gtggacattg ccactgtcta ccagatcttc
     2401 cctgacgaag tgctgggctc agggcagttt ggagtggtct atggaggaaa acaccggaag
     2461 acaggccggg acgtggcagt taaggtcatt gacaaactgc gcttccctac caagcaggag
     2521 agccagctcc ggaatgaagt ggccattctg cagagcctgc ggcatcccgg gatcgtgaac
     2581 ctggagtgca tgttcgagac gcctgagaaa gtgtttgtgg tgatggagaa gctgcatggg
     2641 gacatgttgg agatgatcct gtccagtgag aagggccggc tgcctgagcg cctcaccaag
     2701 ttcctcatca cccagatcct ggtggctttg agacaccttc acttcaagaa cattgtccac
     2761 tgtgacttga aaccagaaaa cgtgttgctg gcatcagcag acccatttcc tcaggtgaag
     2821 ctgtgtgact ttggctttgc tcgcatcatc ggcgagaagt cgttccgccg ctcagtggtg
     2881 ggcacgccgg cctacctggc acccgaggtg ctgctcaacc agggctacaa ccgctcgctg
     2941 gacatgtggt cagtgggcgt gatcatgtac gtcagcctca gcggcacctt ccctttcaac
     3001 gaggatgagg acatcaatga ccagatccag aacgccgcct tcatgtaccc ggccagcccc
     3061 tggagccaca tctcagctgg agccattgac ctcatcaaca acctgctgca ggtgaagatg
     3121 cgcaaacgct acagcgtgga caaatctctc agccacccct ggttacagga gtaccagacg
     3181 tggctggacc tccgagagct ggaggggaag atgggagagc gatacatcac gcatgagagt
     3241 gacgacgcgc gctgggagca gtttgcagca gagcatccgc tgcctgggtc tgggctgccc
     3301 acggacaggg atctcggtgg ggcctgtcca ccacaggacc acgacatgca ggggctggcg
     3361 gagcgcatca gtgttctctg aggtcctgtg ccctcgtcca gctgctgccc tccacagcgg
     3421 ttcttcacag gatcccagca atgaactgtt ctagggaaag tggcttcctg cccaaactgg
     3481 atgggacacg tggggagtgg ggtgggggga gctatttcca aggcccctcc ctgtttcccc
     3541 agcaattaaa acggactcat ctctggcccc atggccttga tctcagca
//
