LOCUS       NM_002744               2385 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens protein kinase C zeta (PRKCZ), transcript variant 1,
            mRNA.
ACCESSION   NM_002744
VERSION     NM_002744.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Mustafa,A., Shabbir,M., Badshah,Y., Khan,K., Abid,F.,
            Trembley,J.H., Afsar,T., Almajwal,A. and Razak,S.
  TITLE     Genetic polymorphism in untranslated regions of PRKCZ influences
            mRNA structure, stability and binding sites
  JOURNAL   BMC Cancer 24 (1), 1147 (2024)
   PUBMED   39272077
  REMARK    GeneRIF: Genetic polymorphism in untranslated regions of PRKCZ
            influences mRNA structure, stability and binding sites.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wang,C., Zhao,X., Zhao,L., Wang,Y., Jia,Y., Zhang,X. and Ma,W.
  TITLE     PKCzeta phosphorylates VASP to mediate chemotaxis in breast cancer
            cells
  JOURNAL   Exp Cell Res 433 (2), 113823 (2023)
   PUBMED   37890607
  REMARK    GeneRIF: PKCzeta phosphorylates VASP to mediate chemotaxis in
            breast cancer cells.
REFERENCE   3  (bases 1 to 2385)
  AUTHORS   Perveen,K., Quach,A., Stark,M.J., Prescott,S., Barry,S.C., Hii,C.S.
            and Ferrante,A.
  TITLE     PKCzeta activation promotes maturation of cord blood T cells
            towards a Th1 IFN-gamma propensity
  JOURNAL   Immunology 170 (3), 359-373 (2023)
   PUBMED   37340593
  REMARK    GeneRIF: PKCzeta activation promotes maturation of cord blood T
            cells towards a Th1 IFN-gamma propensity.
REFERENCE   4  (bases 1 to 2385)
  AUTHORS   Zhang,F., Zheng,Z., Wang,L., Zeng,W., Wei,W., Zhang,C., Zhao,Z. and
            Liang,W.
  TITLE     PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 38 (2023)
   PUBMED   37029374
  REMARK    GeneRIF: PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2385)
  AUTHORS   Shaha,S., Patel,K., Saadat,S., Panahi,S., de Almeida,M.M.,
            Voronova,A. and Riddell,M.
  TITLE     Human placenta and trophoblasts simultaneously express three
            isoforms of atypical protein kinase-c
  JOURNAL   Placenta 119, 39-43 (2022)
   PUBMED   35124330
  REMARK    GeneRIF: Human placenta and trophoblasts simultaneously express
            three isoforms of atypical protein kinase-c.
REFERENCE   6  (bases 1 to 2385)
  AUTHORS   Tippmer,S., Quitterer,U., Kolm,V., Faussner,A., Roscher,A.,
            Mosthaf,L., Muller-Esterl,W. and Haring,H.
  TITLE     Bradykinin induces translocation of the protein kinase C isoforms
            alpha, epsilon, and zeta
  JOURNAL   Eur J Biochem 225 (1), 297-304 (1994)
   PUBMED   7925449
REFERENCE   7  (bases 1 to 2385)
  AUTHORS   el Benna,J., Faust,L.P. and Babior,B.M.
  TITLE     The phosphorylation of the respiratory burst oxidase component
            p47phox during neutrophil activation. Phosphorylation of sites
            recognized by protein kinase C and by proline-directed kinases
  JOURNAL   J Biol Chem 269 (38), 23431-23436 (1994)
   PUBMED   8089108
REFERENCE   8  (bases 1 to 2385)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   9  (bases 1 to 2385)
  AUTHORS   Kawabe,J., Iwami,G., Ebina,T., Ohno,S., Katada,T., Ueda,Y.,
            Homcy,C.J. and Ishikawa,Y.
  TITLE     Differential activation of adenylyl cyclase by protein kinase C
            isoenzymes
  JOURNAL   J Biol Chem 269 (24), 16554-16558 (1994)
   PUBMED   8206971
  REMARK    Erratum:[J Biol Chem 1994 Sep 9;269(36):22912]
REFERENCE   10 (bases 1 to 2385)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL391845.49, BC014270.2 and
            BC008058.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002744.5.
            
            Summary: Protein kinase C (PKC) zeta is a member of the PKC family
            of serine/threonine kinases which are involved in a variety of
            cellular processes such as proliferation, differentiation and
            secretion. Unlike the classical PKC isoenzymes which are
            calcium-dependent, PKC zeta exhibits a kinase activity which is
            independent of calcium and diacylglycerol but not of
            phosphatidylserine. Furthermore, it is insensitive to typical PKC
            inhibitors and cannot be activated by phorbol ester. Unlike the
            classical PKC isoenzymes, it has only a single zinc finger module.
            These structural and biochemical properties indicate that the zeta
            subspecies is related to, but distinct from other isoenzymes of
            PKC. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008058.2, BC014270.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378567.8/ ENSP00000367830.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AL391845.49        90396-90454
            60-2223             BC014270.2         1-2164
            2224-2385           BC008058.2         2112-2273
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2385
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="protein kinase C zeta"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
     exon            1..291
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1999
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /EC_number="2.7.11.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase C zeta type; nPKC-zeta"
                     /codon_start=1
                     /product="protein kinase C zeta type isoform 1"
                     /protein_id="NP_002735.3"
                     /db_xref="CCDS:CCDS37.1"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
                     /translation="MPSRTGPKMEGSGGRVRLKAHYGGDIFITSVDAATTFEELCEEV
                     RDMCRLHQQHPLTLKWVDSEGDPCTVSSQMELEEAFRLARQCRDEGLIIHVFPSTPEQ
                     PGLPCPGEDKSIYRRGARRWRKLYRANGHLFQAKRFNRRAYCGQCSERIWGLARQGYR
                     CINCKLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSS
                     RKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQ
                     IYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVN
                     GGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKL
                     TDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSP
                     FDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTG
                     FSDIKSHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDA
                     IKRIDQSEFEGFEYINPLLLSTEESV"
     exon            292..413
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            414..503
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            504..554
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            555..640
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            641..772
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            773..854
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            855..907
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1096
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1194
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1281
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1417
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1505
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1625
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1705
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1795
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1911
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2385
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2361..2366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="hexamer: AATAAA"
     polyA_site      2385
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="major polyA site"
ORIGIN      
        1 gttcggacac cgccccccgc ccccgccgga cggtcccgcc ccgcgcgccc cccgctcccg
       61 ccccgcgcgc cgccggagtt ccgcggagtt gaccgggtcg gcgccgtcgg tcctgagcgc
      121 tgccttccgc gttccgccgc ggccccacct ggagcccccg ccccgcgcca tggccggagc
      181 tcccggggcg cagcgctgac ggcggcgggg ggagcgcgcc atgcccagca ggaccggccc
      241 caagatggaa gggagcggcg gccgcgtccg cctcaaggcg cattacgggg gggacatctt
      301 catcaccagc gtggacgccg ccacgacctt cgaggagctc tgtgaggaag tgagagacat
      361 gtgtcgtctg caccagcagc acccgctcac cctcaagtgg gtggacagcg aaggtgaccc
      421 ttgcacggtg tcctcccaga tggagctgga agaggctttc cgcctggccc gtcagtgcag
      481 ggatgaaggc ctcatcattc atgttttccc gagcacccct gagcagcctg gcctgccatg
      541 tccgggagaa gacaaatcta tctaccgccg gggagccaga agatggagga agctgtaccg
      601 tgccaacggc cacctcttcc aagccaagcg ctttaacagg agagcgtact gcggtcagtg
      661 cagcgagagg atatggggcc tcgcgaggca aggctacagg tgcatcaact gcaaactgct
      721 ggtccataag cgctgccacg gcctcgtccc gctgacctgc aggaagcata tggattctgt
      781 catgccttcc caagagcctc cagtagacga caagaacgag gacgccgacc ttccttccga
      841 ggagacagat ggaattgctt acatttcctc atcccggaag catgacagca ttaaagacga
      901 ctcggaggac cttaagccag ttatcgatgg gatggatgga atcaaaatct ctcaggggct
      961 tgggctgcag gactttgacc taatcagagt catcgggcgc gggagctacg ccaaggttct
     1021 cctggtgcgg ttgaagaaga atgaccaaat ttacgccatg aaagtggtga agaaagagct
     1081 ggtgcatgat gacgaggata ttgactgggt acagacagag aagcacgtgt ttgagcaggc
     1141 atccagcaac cccttcctgg tcggattaca ctcctgcttc cagacgacaa gtcggttgtt
     1201 cctggtcatt gagtacgtca acggcgggga cctgatgttc cacatgcaga ggcagaggaa
     1261 gctccctgag gagcacgcca ggttctacgc ggccgagatc tgcatcgccc tcaacttcct
     1321 gcacgagagg gggatcatct acagggacct gaagctggac aacgtcctcc tggatgcgga
     1381 cgggcacatc aagctcacag actacggcat gtgcaaggaa ggcctgggcc ctggtgacac
     1441 aacgagcact ttctgcggaa ccccgaatta catcgccccc gaaatcctgc ggggagagga
     1501 gtacgggttc agcgtggact ggtgggcgct gggagtcctc atgtttgaga tgatggccgg
     1561 gcgctccccg ttcgacatca tcaccgacaa cccggacatg aacacagagg actacctttt
     1621 ccaagtgatc ctggagaagc ccatccggat cccccggttc ctgtccgtca aagcctccca
     1681 tgttttaaaa ggatttttaa ataaggaccc caaagagagg ctcggctgcc ggccacagac
     1741 tggattttct gacatcaagt cccacgcgtt cttccgcagc atagactggg acttgctgga
     1801 gaagaagcag gcgctccctc cattccagcc acagatcaca gacgactacg gtctggacaa
     1861 ctttgacaca cagttcacca gcgagcccgt gcagctgacc ccagacgatg aggatgccat
     1921 aaagaggatc gaccagtcag agttcgaagg ctttgagtat atcaacccat tattgctgtc
     1981 caccgaggag tcggtgtgag gccgcgtgcg tctctgtcgt ggacacgcgt gattgaccct
     2041 ttaactgtat ccttaaccac cgcatatgca tgccaggctg ggcacggctc cgagggcggc
     2101 cagggacaga cgcttgcgcc gagaccgcag agggaagcgt cagcgggcgc tgctgggagc
     2161 agaacagtcc ctcacacctg ggcccgggca ggccagcttc gtgctggagg aacttgctgc
     2221 tgtgcctgcg tcgcggcgga tccgcgggga ccctgccgag ggggctgtca tgcggtttcc
     2281 aaggtgcaca ttttccacgg aaacagaact cgatgcactg acctgctccg ccaggaaagt
     2341 gagcgtgtag cgtcctgagg aataaaatgt tccgatgatg tggaa
//
