LOCUS       NM_001350806            2294 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens protein kinase C zeta (PRKCZ), transcript variant 8,
            mRNA.
ACCESSION   NM_001350806 XM_017001804
VERSION     NM_001350806.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Mustafa,A., Shabbir,M., Badshah,Y., Khan,K., Abid,F.,
            Trembley,J.H., Afsar,T., Almajwal,A. and Razak,S.
  TITLE     Genetic polymorphism in untranslated regions of PRKCZ influences
            mRNA structure, stability and binding sites
  JOURNAL   BMC Cancer 24 (1), 1147 (2024)
   PUBMED   39272077
  REMARK    GeneRIF: Genetic polymorphism in untranslated regions of PRKCZ
            influences mRNA structure, stability and binding sites.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Wang,C., Zhao,X., Zhao,L., Wang,Y., Jia,Y., Zhang,X. and Ma,W.
  TITLE     PKCzeta phosphorylates VASP to mediate chemotaxis in breast cancer
            cells
  JOURNAL   Exp Cell Res 433 (2), 113823 (2023)
   PUBMED   37890607
  REMARK    GeneRIF: PKCzeta phosphorylates VASP to mediate chemotaxis in
            breast cancer cells.
REFERENCE   3  (bases 1 to 2294)
  AUTHORS   Perveen,K., Quach,A., Stark,M.J., Prescott,S., Barry,S.C., Hii,C.S.
            and Ferrante,A.
  TITLE     PKCzeta activation promotes maturation of cord blood T cells
            towards a Th1 IFN-gamma propensity
  JOURNAL   Immunology 170 (3), 359-373 (2023)
   PUBMED   37340593
  REMARK    GeneRIF: PKCzeta activation promotes maturation of cord blood T
            cells towards a Th1 IFN-gamma propensity.
REFERENCE   4  (bases 1 to 2294)
  AUTHORS   Zhang,F., Zheng,Z., Wang,L., Zeng,W., Wei,W., Zhang,C., Zhao,Z. and
            Liang,W.
  TITLE     PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 38 (2023)
   PUBMED   37029374
  REMARK    GeneRIF: PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2294)
  AUTHORS   Shaha,S., Patel,K., Saadat,S., Panahi,S., de Almeida,M.M.,
            Voronova,A. and Riddell,M.
  TITLE     Human placenta and trophoblasts simultaneously express three
            isoforms of atypical protein kinase-c
  JOURNAL   Placenta 119, 39-43 (2022)
   PUBMED   35124330
  REMARK    GeneRIF: Human placenta and trophoblasts simultaneously express
            three isoforms of atypical protein kinase-c.
REFERENCE   6  (bases 1 to 2294)
  AUTHORS   Tippmer,S., Quitterer,U., Kolm,V., Faussner,A., Roscher,A.,
            Mosthaf,L., Muller-Esterl,W. and Haring,H.
  TITLE     Bradykinin induces translocation of the protein kinase C isoforms
            alpha, epsilon, and zeta
  JOURNAL   Eur J Biochem 225 (1), 297-304 (1994)
   PUBMED   7925449
REFERENCE   7  (bases 1 to 2294)
  AUTHORS   el Benna,J., Faust,L.P. and Babior,B.M.
  TITLE     The phosphorylation of the respiratory burst oxidase component
            p47phox during neutrophil activation. Phosphorylation of sites
            recognized by protein kinase C and by proline-directed kinases
  JOURNAL   J Biol Chem 269 (38), 23431-23436 (1994)
   PUBMED   8089108
REFERENCE   8  (bases 1 to 2294)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   9  (bases 1 to 2294)
  AUTHORS   Kawabe,J., Iwami,G., Ebina,T., Ohno,S., Katada,T., Ueda,Y.,
            Homcy,C.J. and Ishikawa,Y.
  TITLE     Differential activation of adenylyl cyclase by protein kinase C
            isoenzymes
  JOURNAL   J Biol Chem 269 (24), 16554-16558 (1994)
   PUBMED   8206971
  REMARK    Erratum:[J Biol Chem 1994 Sep 9;269(36):22912]
REFERENCE   10 (bases 1 to 2294)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC314278.1, BC008058.2,
            AL590822.37 and AI186591.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001350806.1.
            
            Summary: Protein kinase C (PKC) zeta is a member of the PKC family
            of serine/threonine kinases which are involved in a variety of
            cellular processes such as proliferation, differentiation and
            secretion. Unlike the classical PKC isoenzymes which are
            calcium-dependent, PKC zeta exhibits a kinase activity which is
            independent of calcium and diacylglycerol but not of
            phosphatidylserine. Furthermore, it is insensitive to typical PKC
            inhibitors and cannot be activated by phorbol ester. Unlike the
            classical PKC isoenzymes, it has only a single zinc finger module.
            These structural and biochemical properties indicate that the zeta
            subspecies is related to, but distinct from other isoenzymes of
            PKC. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1077652.1,
                                           SRR1803616.74578.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-533               DC314278.1         52-584
            534-566             BC008058.2         710-742
            567-763             AL590822.37        3163-3359
            764-1895            BC008058.2         743-1874
            1896-2294           AI186591.1         14-412              c
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2294
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="protein kinase C zeta"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
     exon            1..266
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            267..352
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            353..484
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..763
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            764..816
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1005
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             841..1908
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /EC_number="2.7.11.13"
                     /note="isoform 5 is encoded by transcript variant 8;
                     protein kinase C zeta type; nPKC-zeta"
                     /codon_start=1
                     /product="protein kinase C zeta type isoform 5"
                     /protein_id="NP_001337735.1"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
                     /translation="MDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMK
                     VVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLM
                     FHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGM
                     CKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIIT
                     DNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIK
                     SHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDAIKRID
                     QSEFEGFEYINPLLLSTEESV"
     exon            1006..1103
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1190
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1326
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1414
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1534
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1614
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1704
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1820
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2294
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2270..2275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="hexamer: AATAAA"
     polyA_site      2294
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="major polyA site"
ORIGIN      
        1 agctgctggc tacagcttcc cgggggagcc gggtaccacc cgggcctgga gacatgagga
       61 ggcagggatg tgaggggcgg gggacaggac agccggcctt ccgttaaata tctgctcctc
      121 gcgctcgagc ctccctgcct attgtcgggg ccggaggcga gccgacgcag catcagctcg
      181 tcaacgggaa ggaagatgcc tccctgcacg cccgccgcgc acagagcata aagaatctgc
      241 gctgaggagg caggagaaga aagccgaatc tatctaccgc cggggagcca gaagatggag
      301 gaagctgtac cgtgccaacg gccacctctt ccaagccaag cgctttaaca ggagagcgta
      361 ctgcggtcag tgcagcgaga ggatatgggg cctcgcgagg caaggctaca ggtgcatcaa
      421 ctgcaaactg ctggtccata agcgctgcca cggcctcgtc ccgctgacct gcaggaagca
      481 tatggattct gtcatgcctt cccaagagcc tccagtagac gacaagaacg aggacgccga
      541 ccttccttcc gaggagacag atggaagtag gcgctgcttt cttccggccg ggtagagcct
      601 gggcatcacc tcaccctgct cacctctgcc ttctagccac gagtcctttc tcagtcccat
      661 ctgctctgca ggggtcattg tcttcaagcc tggccaccct tccctggggc tggggatgag
      721 gctctccagg gcctcctctc aatccccggc agagatgagc aggttgctta catttcctca
      781 tcccggaagc atgacagcat taaagacgac tcggaggacc ttaagccagt tatcgatggg
      841 atggatggaa tcaaaatctc tcaggggctt gggctgcagg actttgacct aatcagagtc
      901 atcgggcgcg ggagctacgc caaggttctc ctggtgcggt tgaagaagaa tgaccaaatt
      961 tacgccatga aagtggtgaa gaaagagctg gtgcatgatg acgaggatat tgactgggta
     1021 cagacagaga agcacgtgtt tgagcaggca tccagcaacc ccttcctggt cggattacac
     1081 tcctgcttcc agacgacaag tcggttgttc ctggtcattg agtacgtcaa cggcggggac
     1141 ctgatgttcc acatgcagag gcagaggaag ctccctgagg agcacgccag gttctacgcg
     1201 gccgagatct gcatcgccct caacttcctg cacgagaggg ggatcatcta cagggacctg
     1261 aagctggaca acgtcctcct ggatgcggac gggcacatca agctcacaga ctacggcatg
     1321 tgcaaggaag gcctgggccc tggtgacaca acgagcactt tctgcggaac cccgaattac
     1381 atcgcccccg aaatcctgcg gggagaggag tacgggttca gcgtggactg gtgggcgctg
     1441 ggagtcctca tgtttgagat gatggccggg cgctccccgt tcgacatcat caccgacaac
     1501 ccggacatga acacagagga ctaccttttc caagtgatcc tggagaagcc catccggatc
     1561 ccccggttcc tgtccgtcaa agcctcccat gttttaaaag gatttttaaa taaggacccc
     1621 aaagagaggc tcggctgccg gccacagact ggattttctg acatcaagtc ccacgcgttc
     1681 ttccgcagca tagactggga cttgctggag aagaagcagg cgctccctcc attccagcca
     1741 cagatcacag acgactacgg tctggacaac tttgacacac agttcaccag cgagcccgtg
     1801 cagctgaccc cagacgatga ggatgccata aagaggatcg accagtcaga gttcgaaggc
     1861 tttgagtata tcaacccatt attgctgtcc accgaggagt cggtgtgagg ccgcgtgcgt
     1921 ctctgtcgtg gacacgcgtg attgaccctt taactgtatc cttaaccacc gcatatgcat
     1981 gccaggctgg gcacggctcc gagggcggcc agggacagac gcttgcgccg agaccgcaga
     2041 gggaagcgtc agcgggcgct gctgggagca gaacagtccc tcacacctgg gcccgggcag
     2101 gccagcttcg tgctggagga acttgctgct gtgcctgcgt cgcggcggat ccgcggggac
     2161 cctgccgagg gggctgtcat gcggtttcca aggtgcacat tttccacgga aacagaactc
     2221 gatgcactga cctgctccgc caggaaagtg agcgtgtagc gtcctgagga ataaaatgtt
     2281 ccgatgatgt ggaa
//
