LOCUS       NM_001350805            2233 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens protein kinase C zeta (PRKCZ), transcript variant 7,
            mRNA.
ACCESSION   NM_001350805 XM_017001805
VERSION     NM_001350805.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Mustafa,A., Shabbir,M., Badshah,Y., Khan,K., Abid,F.,
            Trembley,J.H., Afsar,T., Almajwal,A. and Razak,S.
  TITLE     Genetic polymorphism in untranslated regions of PRKCZ influences
            mRNA structure, stability and binding sites
  JOURNAL   BMC Cancer 24 (1), 1147 (2024)
   PUBMED   39272077
  REMARK    GeneRIF: Genetic polymorphism in untranslated regions of PRKCZ
            influences mRNA structure, stability and binding sites.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wang,C., Zhao,X., Zhao,L., Wang,Y., Jia,Y., Zhang,X. and Ma,W.
  TITLE     PKCzeta phosphorylates VASP to mediate chemotaxis in breast cancer
            cells
  JOURNAL   Exp Cell Res 433 (2), 113823 (2023)
   PUBMED   37890607
  REMARK    GeneRIF: PKCzeta phosphorylates VASP to mediate chemotaxis in
            breast cancer cells.
REFERENCE   3  (bases 1 to 2233)
  AUTHORS   Perveen,K., Quach,A., Stark,M.J., Prescott,S., Barry,S.C., Hii,C.S.
            and Ferrante,A.
  TITLE     PKCzeta activation promotes maturation of cord blood T cells
            towards a Th1 IFN-gamma propensity
  JOURNAL   Immunology 170 (3), 359-373 (2023)
   PUBMED   37340593
  REMARK    GeneRIF: PKCzeta activation promotes maturation of cord blood T
            cells towards a Th1 IFN-gamma propensity.
REFERENCE   4  (bases 1 to 2233)
  AUTHORS   Zhang,F., Zheng,Z., Wang,L., Zeng,W., Wei,W., Zhang,C., Zhao,Z. and
            Liang,W.
  TITLE     PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 38 (2023)
   PUBMED   37029374
  REMARK    GeneRIF: PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2233)
  AUTHORS   Shaha,S., Patel,K., Saadat,S., Panahi,S., de Almeida,M.M.,
            Voronova,A. and Riddell,M.
  TITLE     Human placenta and trophoblasts simultaneously express three
            isoforms of atypical protein kinase-c
  JOURNAL   Placenta 119, 39-43 (2022)
   PUBMED   35124330
  REMARK    GeneRIF: Human placenta and trophoblasts simultaneously express
            three isoforms of atypical protein kinase-c.
REFERENCE   6  (bases 1 to 2233)
  AUTHORS   Tippmer,S., Quitterer,U., Kolm,V., Faussner,A., Roscher,A.,
            Mosthaf,L., Muller-Esterl,W. and Haring,H.
  TITLE     Bradykinin induces translocation of the protein kinase C isoforms
            alpha, epsilon, and zeta
  JOURNAL   Eur J Biochem 225 (1), 297-304 (1994)
   PUBMED   7925449
REFERENCE   7  (bases 1 to 2233)
  AUTHORS   el Benna,J., Faust,L.P. and Babior,B.M.
  TITLE     The phosphorylation of the respiratory burst oxidase component
            p47phox during neutrophil activation. Phosphorylation of sites
            recognized by protein kinase C and by proline-directed kinases
  JOURNAL   J Biol Chem 269 (38), 23431-23436 (1994)
   PUBMED   8089108
REFERENCE   8  (bases 1 to 2233)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   9  (bases 1 to 2233)
  AUTHORS   Kawabe,J., Iwami,G., Ebina,T., Ohno,S., Katada,T., Ueda,Y.,
            Homcy,C.J. and Ishikawa,Y.
  TITLE     Differential activation of adenylyl cyclase by protein kinase C
            isoenzymes
  JOURNAL   J Biol Chem 269 (24), 16554-16558 (1994)
   PUBMED   8206971
  REMARK    Erratum:[J Biol Chem 1994 Sep 9;269(36):22912]
REFERENCE   10 (bases 1 to 2233)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC328654.1, BC008058.2,
            AL590822.37 and AI186591.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001350805.1.
            
            Summary: Protein kinase C (PKC) zeta is a member of the PKC family
            of serine/threonine kinases which are involved in a variety of
            cellular processes such as proliferation, differentiation and
            secretion. Unlike the classical PKC isoenzymes which are
            calcium-dependent, PKC zeta exhibits a kinase activity which is
            independent of calcium and diacylglycerol but not of
            phosphatidylserine. Furthermore, it is insensitive to typical PKC
            inhibitors and cannot be activated by phorbol ester. Unlike the
            classical PKC isoenzymes, it has only a single zinc finger module.
            These structural and biochemical properties indicate that the zeta
            subspecies is related to, but distinct from other isoenzymes of
            PKC. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.89719.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145544, SAMEA2157437
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-450               DC328654.1         45-494
            451-505             BC008058.2         688-742
            506-702             AL590822.37        3163-3359
            703-1834            BC008058.2         743-1874
            1835-2233           AI186591.1         14-412              c
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2233
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="protein kinase C zeta"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
     exon            1..205
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            206..291
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            292..423
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            424..702
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            703..755
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            756..944
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             780..1847
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /EC_number="2.7.11.13"
                     /note="isoform 5 is encoded by transcript variant 7;
                     protein kinase C zeta type; nPKC-zeta"
                     /codon_start=1
                     /product="protein kinase C zeta type isoform 5"
                     /protein_id="NP_001337734.1"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
                     /translation="MDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMK
                     VVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLM
                     FHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGM
                     CKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIIT
                     DNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIK
                     SHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDAIKRID
                     QSEFEGFEYINPLLLSTEESV"
     exon            945..1042
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1129
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1265
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1353
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1473
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1553
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1643
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1759
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..2233
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2209..2214
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="hexamer: AATAAA"
     polyA_site      2233
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="major polyA site"
ORIGIN      
        1 gcacagatgc tccgggcagc aacacggctg gtgccacggc ccggggaagg cgtgcggctg
       61 cagcagctcc cagcaatgtc aggggaaacg cagtgagagg ctgttgtttt gcgggtgaca
      121 gatttttaga aaaataaggc tgtggaggga ccttctgagc gaggcagggg ctgctggagg
      181 gacatgctca ccccgaggac ggatgaatct atctaccgcc ggggagccag aagatggagg
      241 aagctgtacc gtgccaacgg ccacctcttc caagccaagc gctttaacag gagagcgtac
      301 tgcggtcagt gcagcgagag gatatggggc ctcgcgaggc aaggctacag gtgcatcaac
      361 tgcaaactgc tggtccataa gcgctgccac ggcctcgtcc cgctgacctg caggaagcat
      421 atggattctg tcatgccttc ccaagagcct ccagtagacg acaagaacga ggacgccgac
      481 cttccttccg aggagacaga tggaagtagg cgctgctttc ttccggccgg gtagagcctg
      541 ggcatcacct caccctgctc acctctgcct tctagccacg agtcctttct cagtcccatc
      601 tgctctgcag gggtcattgt cttcaagcct ggccaccctt ccctggggct ggggatgagg
      661 ctctccaggg cctcctctca atccccggca gagatgagca ggttgcttac atttcctcat
      721 cccggaagca tgacagcatt aaagacgact cggaggacct taagccagtt atcgatggga
      781 tggatggaat caaaatctct caggggcttg ggctgcagga ctttgaccta atcagagtca
      841 tcgggcgcgg gagctacgcc aaggttctcc tggtgcggtt gaagaagaat gaccaaattt
      901 acgccatgaa agtggtgaag aaagagctgg tgcatgatga cgaggatatt gactgggtac
      961 agacagagaa gcacgtgttt gagcaggcat ccagcaaccc cttcctggtc ggattacact
     1021 cctgcttcca gacgacaagt cggttgttcc tggtcattga gtacgtcaac ggcggggacc
     1081 tgatgttcca catgcagagg cagaggaagc tccctgagga gcacgccagg ttctacgcgg
     1141 ccgagatctg catcgccctc aacttcctgc acgagagggg gatcatctac agggacctga
     1201 agctggacaa cgtcctcctg gatgcggacg ggcacatcaa gctcacagac tacggcatgt
     1261 gcaaggaagg cctgggccct ggtgacacaa cgagcacttt ctgcggaacc ccgaattaca
     1321 tcgcccccga aatcctgcgg ggagaggagt acgggttcag cgtggactgg tgggcgctgg
     1381 gagtcctcat gtttgagatg atggccgggc gctccccgtt cgacatcatc accgacaacc
     1441 cggacatgaa cacagaggac taccttttcc aagtgatcct ggagaagccc atccggatcc
     1501 cccggttcct gtccgtcaaa gcctcccatg ttttaaaagg atttttaaat aaggacccca
     1561 aagagaggct cggctgccgg ccacagactg gattttctga catcaagtcc cacgcgttct
     1621 tccgcagcat agactgggac ttgctggaga agaagcaggc gctccctcca ttccagccac
     1681 agatcacaga cgactacggt ctggacaact ttgacacaca gttcaccagc gagcccgtgc
     1741 agctgacccc agacgatgag gatgccataa agaggatcga ccagtcagag ttcgaaggct
     1801 ttgagtatat caacccatta ttgctgtcca ccgaggagtc ggtgtgaggc cgcgtgcgtc
     1861 tctgtcgtgg acacgcgtga ttgacccttt aactgtatcc ttaaccaccg catatgcatg
     1921 ccaggctggg cacggctccg agggcggcca gggacagacg cttgcgccga gaccgcagag
     1981 ggaagcgtca gcgggcgctg ctgggagcag aacagtccct cacacctggg cccgggcagg
     2041 ccagcttcgt gctggaggaa cttgctgctg tgcctgcgtc gcggcggatc cgcggggacc
     2101 ctgccgaggg ggctgtcatg cggtttccaa ggtgcacatt ttccacggaa acagaactcg
     2161 atgcactgac ctgctccgcc aggaaagtga gcgtgtagcg tcctgaggaa taaaatgttc
     2221 cgatgatgtg gaa
//
