LOCUS       NM_001033581            2097 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens protein kinase C zeta (PRKCZ), transcript variant 2,
            mRNA.
ACCESSION   NM_001033581
VERSION     NM_001033581.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Mustafa,A., Shabbir,M., Badshah,Y., Khan,K., Abid,F.,
            Trembley,J.H., Afsar,T., Almajwal,A. and Razak,S.
  TITLE     Genetic polymorphism in untranslated regions of PRKCZ influences
            mRNA structure, stability and binding sites
  JOURNAL   BMC Cancer 24 (1), 1147 (2024)
   PUBMED   39272077
  REMARK    GeneRIF: Genetic polymorphism in untranslated regions of PRKCZ
            influences mRNA structure, stability and binding sites.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wang,C., Zhao,X., Zhao,L., Wang,Y., Jia,Y., Zhang,X. and Ma,W.
  TITLE     PKCzeta phosphorylates VASP to mediate chemotaxis in breast cancer
            cells
  JOURNAL   Exp Cell Res 433 (2), 113823 (2023)
   PUBMED   37890607
  REMARK    GeneRIF: PKCzeta phosphorylates VASP to mediate chemotaxis in
            breast cancer cells.
REFERENCE   3  (bases 1 to 2097)
  AUTHORS   Perveen,K., Quach,A., Stark,M.J., Prescott,S., Barry,S.C., Hii,C.S.
            and Ferrante,A.
  TITLE     PKCzeta activation promotes maturation of cord blood T cells
            towards a Th1 IFN-gamma propensity
  JOURNAL   Immunology 170 (3), 359-373 (2023)
   PUBMED   37340593
  REMARK    GeneRIF: PKCzeta activation promotes maturation of cord blood T
            cells towards a Th1 IFN-gamma propensity.
REFERENCE   4  (bases 1 to 2097)
  AUTHORS   Zhang,F., Zheng,Z., Wang,L., Zeng,W., Wei,W., Zhang,C., Zhao,Z. and
            Liang,W.
  TITLE     PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 38 (2023)
   PUBMED   37029374
  REMARK    GeneRIF: PKC-zeta mediated reduction of the extracellular
            vesicles-associated TGF-beta1 overcomes radiotherapy resistance in
            breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2097)
  AUTHORS   Shaha,S., Patel,K., Saadat,S., Panahi,S., de Almeida,M.M.,
            Voronova,A. and Riddell,M.
  TITLE     Human placenta and trophoblasts simultaneously express three
            isoforms of atypical protein kinase-c
  JOURNAL   Placenta 119, 39-43 (2022)
   PUBMED   35124330
  REMARK    GeneRIF: Human placenta and trophoblasts simultaneously express
            three isoforms of atypical protein kinase-c.
REFERENCE   6  (bases 1 to 2097)
  AUTHORS   Tippmer,S., Quitterer,U., Kolm,V., Faussner,A., Roscher,A.,
            Mosthaf,L., Muller-Esterl,W. and Haring,H.
  TITLE     Bradykinin induces translocation of the protein kinase C isoforms
            alpha, epsilon, and zeta
  JOURNAL   Eur J Biochem 225 (1), 297-304 (1994)
   PUBMED   7925449
REFERENCE   7  (bases 1 to 2097)
  AUTHORS   el Benna,J., Faust,L.P. and Babior,B.M.
  TITLE     The phosphorylation of the respiratory burst oxidase component
            p47phox during neutrophil activation. Phosphorylation of sites
            recognized by protein kinase C and by proline-directed kinases
  JOURNAL   J Biol Chem 269 (38), 23431-23436 (1994)
   PUBMED   8089108
REFERENCE   8  (bases 1 to 2097)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   9  (bases 1 to 2097)
  AUTHORS   Kawabe,J., Iwami,G., Ebina,T., Ohno,S., Katada,T., Ueda,Y.,
            Homcy,C.J. and Ishikawa,Y.
  TITLE     Differential activation of adenylyl cyclase by protein kinase C
            isoenzymes
  JOURNAL   J Biol Chem 269 (24), 16554-16558 (1994)
   PUBMED   8206971
  REMARK    Erratum:[J Biol Chem 1994 Sep 9;269(36):22912]
REFERENCE   10 (bases 1 to 2097)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB152283.1 and BC008058.2.
            
            On Aug 14, 2020 this sequence version replaced NM_001033581.2.
            
            Summary: Protein kinase C (PKC) zeta is a member of the PKC family
            of serine/threonine kinases which are involved in a variety of
            cellular processes such as proliferation, differentiation and
            secretion. Unlike the classical PKC isoenzymes which are
            calcium-dependent, PKC zeta exhibits a kinase activity which is
            independent of calcium and diacylglycerol but not of
            phosphatidylserine. Furthermore, it is insensitive to typical PKC
            inhibitors and cannot be activated by phorbol ester. Unlike the
            classical PKC isoenzymes, it has only a single zinc finger module.
            These structural and biochemical properties indicate that the zeta
            subspecies is related to, but distinct from other isoenzymes of
            PKC. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and has
            multiple coding region differences, compared to variant 1. These
            differences cause translation initiation from a downstream ATG and
            an isoform (2) with a shorter N-terminus, compared to isoform 1.
            Variants 2 and 3 encode the same isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK294649.1, SRR13316199.255236.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-508               CB152283.1         18-525
            509-2097            BC008058.2         685-2273
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2097
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="protein kinase C zeta"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
     exon            1..266
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            267..352
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            353..484
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             482..1711
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /EC_number="2.7.11.13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein kinase C zeta type; nPKC-zeta"
                     /codon_start=1
                     /product="protein kinase C zeta type isoform 2"
                     /protein_id="NP_001028753.1"
                     /db_xref="CCDS:CCDS41229.1"
                     /db_xref="GeneID:5590"
                     /db_xref="HGNC:HGNC:9412"
                     /db_xref="MIM:176982"
                     /translation="MDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSSRKHDSIKDD
                     SEDLKPVIDGMDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKK
                     ELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQ
                     RQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEG
                     LGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPD
                     MNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAF
                     FRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDAIKRIDQSEF
                     EGFEYINPLLLSTEESV"
     exon            485..566
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            567..619
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            620..808
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            809..906
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            907..993
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1129
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1217
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1337
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1417
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1507
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1623
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..2097
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2073..2078
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="hexamer: AATAAA"
     polyA_site      2097
                     /gene="PRKCZ"
                     /gene_synonym="PKC-ZETA; PKC2"
                     /note="major polyA site"
ORIGIN      
        1 agctgctggc tacagcttcc cgggggagcc gggtaccacc cgggcctgga gacatgagga
       61 ggcagggatg tgaggggcgg gggacaggac agccggcctt ccgttaaata tctgctcctc
      121 gcgctcgagc ctccctgcct attgtcgggg ccggaggcga gccgacgcag catcagctcg
      181 tcaacgggaa ggaagatgcc tccctgcacg cccgccgcgc acagagcata aagaatctgc
      241 gctgaggagg caggagaaga aagccgaatc tatctaccgc cggggagcca gaagatggag
      301 gaagctgtac cgtgccaacg gccacctctt ccaagccaag cgctttaaca ggagagcgta
      361 ctgcggtcag tgcagcgaga ggatatgggg cctcgcgagg caaggctaca ggtgcatcaa
      421 ctgcaaactg ctggtccata agcgctgcca cggcctcgtc ccgctgacct gcaggaagca
      481 tatggattct gtcatgcctt cccaagagcc tccagtagac gacaagaacg aggacgccga
      541 ccttccttcc gaggagacag atggaattgc ttacatttcc tcatcccgga agcatgacag
      601 cattaaagac gactcggagg accttaagcc agttatcgat gggatggatg gaatcaaaat
      661 ctctcagggg cttgggctgc aggactttga cctaatcaga gtcatcgggc gcgggagcta
      721 cgccaaggtt ctcctggtgc ggttgaagaa gaatgaccaa atttacgcca tgaaagtggt
      781 gaagaaagag ctggtgcatg atgacgagga tattgactgg gtacagacag agaagcacgt
      841 gtttgagcag gcatccagca accccttcct ggtcggatta cactcctgct tccagacgac
      901 aagtcggttg ttcctggtca ttgagtacgt caacggcggg gacctgatgt tccacatgca
      961 gaggcagagg aagctccctg aggagcacgc caggttctac gcggccgaga tctgcatcgc
     1021 cctcaacttc ctgcacgaga gggggatcat ctacagggac ctgaagctgg acaacgtcct
     1081 cctggatgcg gacgggcaca tcaagctcac agactacggc atgtgcaagg aaggcctggg
     1141 ccctggtgac acaacgagca ctttctgcgg aaccccgaat tacatcgccc ccgaaatcct
     1201 gcggggagag gagtacgggt tcagcgtgga ctggtgggcg ctgggagtcc tcatgtttga
     1261 gatgatggcc gggcgctccc cgttcgacat catcaccgac aacccggaca tgaacacaga
     1321 ggactacctt ttccaagtga tcctggagaa gcccatccgg atcccccggt tcctgtccgt
     1381 caaagcctcc catgttttaa aaggattttt aaataaggac cccaaagaga ggctcggctg
     1441 ccggccacag actggatttt ctgacatcaa gtcccacgcg ttcttccgca gcatagactg
     1501 ggacttgctg gagaagaagc aggcgctccc tccattccag ccacagatca cagacgacta
     1561 cggtctggac aactttgaca cacagttcac cagcgagccc gtgcagctga ccccagacga
     1621 tgaggatgcc ataaagagga tcgaccagtc agagttcgaa ggctttgagt atatcaaccc
     1681 attattgctg tccaccgagg agtcggtgtg aggccgcgtg cgtctctgtc gtggacacgc
     1741 gtgattgacc ctttaactgt atccttaacc accgcatatg catgccaggc tgggcacggc
     1801 tccgagggcg gccagggaca gacgcttgcg ccgagaccgc agagggaagc gtcagcgggc
     1861 gctgctggga gcagaacagt ccctcacacc tgggcccggg caggccagct tcgtgctgga
     1921 ggaacttgct gctgtgcctg cgtcgcggcg gatccgcggg gaccctgccg agggggctgt
     1981 catgcggttt ccaaggtgca cattttccac ggaaacagaa ctcgatgcac tgacctgctc
     2041 cgccaggaaa gtgagcgtgt agcgtcctga ggaataaaat gttccgatga tgtggaa
//
