LOCUS       NM_006257               3255 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens protein kinase C theta (PRKCQ), transcript variant 1,
            mRNA.
ACCESSION   NM_006257
VERSION     NM_006257.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Chmiest,D., Podavini,S., Ioannidou,K., Vallois,D., Decaillet,C.,
            Gonzalez,M., Quadroni,M., Blackney,K., Schairer,R., de Leval,L. and
            Thome,M.
  TITLE     PD1 inhibits PKCtheta;-dependent phosphorylation of
            cytoskeleton-related proteins and immune synapse formation
  JOURNAL   Blood Adv 8 (11), 2908-2923 (2024)
   PUBMED   38513140
  REMARK    GeneRIF: PD1 inhibits PKCtheta;-dependent phosphorylation of
            cytoskeleton-related proteins and immune synapse formation.
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Anto,N.P., Muraleedharan,A., Nath,P.R., Sun,Z., Keasar,C.,
            Livneh,E., Braiman,A., Altman,A., Kong,K.F. and Isakov,N.
  TITLE     The Peptidyl-Prolyl cis-trans isomerase, Pin1, associates with
            Protein Kinase C theta; via a critical Phospho-Thr-Pro motif in the
            V3 regulatory domain
  JOURNAL   Front Immunol 14, 1126464 (2023)
   PUBMED   36969236
  REMARK    GeneRIF: The Peptidyl-Prolyl cis-trans isomerase, Pin1, associates
            with Protein Kinase C theta; via a critical Phospho-Thr-Pro motif
            in the V3 regulatory domain.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3255)
  AUTHORS   Xu,Q., Yu,J., Jia,G., Li,Z. and Xiong,H.
  TITLE     Crocin attenuates NF-kappaB-mediated inflammation and proliferation
            in breast cancer cells by down-regulating PRKCQ
  JOURNAL   Cytokine 154, 155888 (2022)
   PUBMED   35447530
  REMARK    GeneRIF: Crocin attenuates NF-kappaB-mediated inflammation and
            proliferation in breast cancer cells by down-regulating PRKCQ.
REFERENCE   4  (bases 1 to 3255)
  AUTHORS   Jayaram,D.R., Frost,S., Argov,C., Liju,V.B., Anto,N.P.,
            Muraleedharan,A., Ben-Ari,A., Sinay,R., Smoly,I., Novoplansky,O.,
            Isakov,N., Toiber,D., Keasar,C., Elkabets,M., Yeger-Lotem,E. and
            Livneh,E.
  TITLE     Unraveling the hidden role of a uORF-encoded peptide as a kinase
            inhibitor of PKCs
  JOURNAL   Proc Natl Acad Sci U S A 118 (40) (2021)
   PUBMED   34593629
REFERENCE   5  (bases 1 to 3255)
  AUTHORS   Salem,S. and Mosaad,R.
  TITLE     Crosstalk between miR-203 and PKCtheta; regulates breast cancer
            stem cell markers
  JOURNAL   Ann Hum Genet 85 (3-4), 105-114 (2021)
   PUBMED   33576006
  REMARK    GeneRIF: Crosstalk between miR-203 and PKCtheta regulates breast
            cancer stem cell markers.
REFERENCE   6  (bases 1 to 3255)
  AUTHORS   Conant,K., Ma,M., Nath,A. and Major,E.O.
  TITLE     Extracellular human immunodeficiency virus type 1 Tat protein is
            associated with an increase in both NF-kappa B binding and protein
            kinase C activity in primary human astrocytes
  JOURNAL   J Virol 70 (3), 1384-1389 (1996)
   PUBMED   8627654
REFERENCE   7  (bases 1 to 3255)
  AUTHORS   Erdel,M., Baier-Bitterlich,G., Duba,C., Isakov,N., Altman,A.,
            Utermann,G. and Baier,G.
  TITLE     Mapping of the human protein kinase C-theta (PRKCQ) gene locus to
            the short arm of chromosome 10 (10p15) by FISH
  JOURNAL   Genomics 25 (2), 595-597 (1995)
   PUBMED   7790001
REFERENCE   8  (bases 1 to 3255)
  AUTHORS   Chang,J.D., Xu,Y., Raychowdhury,M.K. and Ware,J.A.
  TITLE     Molecular cloning and expression of a cDNA encoding a novel
            isoenzyme of protein kinase C (nPKC). A new member of the nPKC
            family expressed in skeletal muscle, megakaryoblastic cells, and
            platelets
  JOURNAL   J Biol Chem 268 (19), 14208-14214 (1993)
   PUBMED   7686153
  REMARK    Erratum:[J Biol Chem. 1994 Dec 9;269(49):31322.. PMID: 16964635]
REFERENCE   9  (bases 1 to 3255)
  AUTHORS   Baier,G., Telford,D., Giampa,L., Coggeshall,K.M.,
            Baier-Bitterlich,G., Isakov,N. and Altman,A.
  TITLE     Molecular cloning and characterization of PKC theta, a novel member
            of the protein kinase C (PKC) gene family expressed predominantly
            in hematopoietic cells
  JOURNAL   J Biol Chem 268 (7), 4997-5004 (1993)
   PUBMED   8444877
REFERENCE   10 (bases 1 to 3255)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK308099.1, AK312411.1, AK293935.1, BX647657.1, AA234440.1 and
            R40214.1.
            
            On May 17, 2019 this sequence version replaced NM_006257.4.
            
            Summary: Protein kinase C (PKC) is a family of serine- and
            threonine-specific protein kinases that can be activated by calcium
            and the second messenger diacylglycerol. PKC family members
            phosphorylate a wide variety of protein targets and are known to be
            involved in diverse cellular signaling pathways. PKC family members
            also serve as major receptors for phorbol esters, a class of tumor
            promoters. Each member of the PKC family has a specific expression
            profile and is believed to play a distinct role. The protein
            encoded by this gene is one of the PKC family members. It is a
            calcium-independent and phospholipid-dependent protein kinase. This
            kinase is important for T-cell activation. It is required for the
            activation of the transcription factors NF-kappaB and AP-1, and may
            link the T cell receptor (TCR) signaling complex to the activation
            of the transcription factors. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 5 both encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L01087.1, AK312411.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263125.10/ ENSP00000263125.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AK308099.1         1-25
            26-1443             AK312411.1         1-1418
            1444-2196           AK312411.1         1420-2172
            2197-2382           AK293935.1         2099-2284
            2383-3209           BX647657.1         2493-3319
            3210-3210           AA234440.1         49-49               c
            3211-3255           R40214.1           16-60               c
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p15.1"
     gene            1..3255
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /note="protein kinase C theta"
                     /db_xref="GeneID:5588"
                     /db_xref="HGNC:HGNC:9410"
                     /db_xref="MIM:600448"
     exon            1..66
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            67..193
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            194..393
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            394..454
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            455..617
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            618..649
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            650..735
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            736..865
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            866..975
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1093
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1254
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1428
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1520
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1583
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1722
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1911
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2040
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..3255
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2743..2748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /note="hexamer: AATAAA"
     polyA_site      2765
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /note="major polyA site"
     regulatory      3192..3197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
                     /note="hexamer: GATAAA"
     polyA_site      3236
                     /gene="PRKCQ"
                     /gene_synonym="nPKC-theta; PRKCT"
ORIGIN      
        1 agtccccgcg cagtccccgc gcagtcccag cgccaccggg cagcagcggc gccgtgctcg
       61 ctccagggcg caaccatgtc gccatttctt cggattggct tgtccaactt tgactgcggg
      121 tcctgccagt cttgtcaggg cgaggctgtt aacccttact gtgctgtgct cgtcaaagag
      181 tatgtcgaat cagagaacgg gcagatgtat atccagaaaa agcctaccat gtacccaccc
      241 tgggacagca cttttgatgc ccatatcaac aagggaagag tcatgcagat cattgtgaaa
      301 ggcaaaaacg tggacctcat ctctgaaacc accgtggagc tctactcgct ggctgagagg
      361 tgcaggaaga acaacgggaa gacagaaata tggttagagc tgaaacctca aggccgaatg
      421 ctaatgaatg caagatactt tctggaaatg agtgacacaa aggacatgaa tgaatttgag
      481 acggaaggct tctttgcttt gcatcagcgc cggggtgcca tcaagcaggc aaaggtccac
      541 cacgtcaagt gccacgagtt cactgccacc ttcttcccac agcccacatt ttgctctgtc
      601 tgccacgagt ttgtctgggg cctgaacaaa cagggctacc agtgccgaca atgcaatgca
      661 gcaattcaca agaagtgtat tgataaagtt atagcaaagt gcacaggatc agctatcaat
      721 agccgagaaa ccatgttcca caaggagaga ttcaaaattg acatgccaca cagatttaaa
      781 gtctacaatt acaagagccc gaccttctgt gaacactgtg ggaccctgct gtggggactg
      841 gcacggcaag gactcaagtg tgatgcatgt ggcatgaatg tgcatcatag atgccagaca
      901 aaggtggcca acctttgtgg cataaaccag aagctaatgg ctgaagcgct ggccatgatt
      961 gagagcactc aacaggctcg ctgcttaaga gatactgaac agatcttcag agaaggtccg
     1021 gttgaaattg gtctcccatg ctccatcaaa aatgaagcaa ggccgccatg tttaccgaca
     1081 ccgggaaaaa gagagcctca gggcatttcc tgggagtctc cgttggatga ggtggataaa
     1141 atgtgccatc ttccagaacc tgaactgaac aaagaaagac catctctgca gattaaacta
     1201 aaaattgagg attttatctt gcacaaaatg ttggggaaag gaagttttgg caaggtcttc
     1261 ctggcagaat tcaagaaaac caatcaattt ttcgcaataa aggccttaaa gaaagatgtg
     1321 gtcttgatgg acgatgatgt tgagtgcacg atggtagaga agagagttct ttccttggcc
     1381 tgggagcatc cgtttctgac gcacatgttt tgtacattcc agaccaagga aaacctcttt
     1441 tttgtgatgg agtacctcaa cggaggggac ttaatgtacc acatccaaag ctgccacaag
     1501 ttcgaccttt ccagagcgac gttttatgct gctgaaatca ttcttggtct gcagttcctt
     1561 cattccaaag gaatagtcta cagggacctg aagctagata acatcctgtt agacaaagat
     1621 ggacatatca agatcgcgga ttttggaatg tgcaaggaga acatgttagg agatgccaag
     1681 acgaatacct tctgtgggac acctgactac atcgccccag agatcttgct gggtcagaaa
     1741 tacaaccact ctgtggactg gtggtccttc ggggttctcc tttatgaaat gctgattggt
     1801 cagtcgcctt tccacgggca ggatgaggag gagctcttcc actccatccg catggacaat
     1861 cccttttacc cacggtggct ggagaaggaa gcaaaggacc ttctggtgaa gctcttcgtg
     1921 cgagaacctg agaagaggct gggcgtgagg ggagacatcc gccagcaccc tttgtttcgg
     1981 gagatcaact gggaggaact tgaacggaag gagattgacc caccgttccg gccgaaagtg
     2041 aaatcaccat ttgactgcag caatttcgac aaagaattct taaacgagaa gccccggctg
     2101 tcatttgccg acagagcact gatcaacagc atggaccaga atatgttcag gaacttttcc
     2161 ttcatgaacc ccgggatgga gcggctgata tcctgaatct tgcccctcca gagacaggaa
     2221 agaatttgcc ttctccctgg gaactggttc aagagacact gcttgggttc ctttttcaac
     2281 ttggaaaaag aaagaaacac tcaacaataa agactgagac ccgttcgccc ccatgtgact
     2341 tttatctgta gcagaaacca agtctacttc actaatgacg atgccgtgtg tctcgtctcc
     2401 tgacatgtct cacagacgct cctgaagtta ggtcattact aaccatagtt atttacttga
     2461 aagatgggtc tccgcacttg gaaaggtttc aagacttgat actgcaataa attatggctc
     2521 ttcacctggg cgccaactgc tgatcaatga aatgcttgtt gaatcagggg caaacggagt
     2581 acagacgtct caagactgaa acggccccat tgcctggtct agtagcggat ctcactcagc
     2641 cgcagacaag taatcactaa cccgttttat tctattccta tctgtggatg tgtaaatggc
     2701 tggggggcca gccctggata ggtttttatg ggaattcttt acaataaaca tagcttgtaa
     2761 cttgagatct acaaatccat tcatcctgat tgggcatgaa atccatggtc aagaggacaa
     2821 gtggaaagtg agagggaagg tttgctagac accttcgctt gttatcttgt caagatagaa
     2881 aagatagtat catttcaccc ttgccagtaa aaacctttcc atccacccat tctcagcaga
     2941 ctccagtatt ggcacagtca ctcactgcca ttctcacact ataacaagaa aagaaatgaa
     3001 gtgcataagt ctcctgggaa aagaacctta accccttctc gtgccatgac tggtgatttc
     3061 atgactcata agcccctccg taggcatcat tcaagatcaa tggcccatgc atgctgtttg
     3121 cagcagtcaa ttgagttgaa ttagaattcc aaccatacat tttaaaggta tttgtgctgt
     3181 gtgtatattt tgataaaatg ttgtgacttc atggcaaaca ggtggatgtg taaaaatgga
     3241 ataaaaaaaa aaaaa
//
