LOCUS       NM_005400               5749 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens protein kinase C epsilon (PRKCE), mRNA.
ACCESSION   NM_005400
VERSION     NM_005400.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Li,X., Wang,J., Liao,C., Yang,X., Zhao,Z., Liu,Y., Xue,Q., Luo,Y.,
            Liu,X. and Liu,Z.
  TITLE     The binding of PKCepsilon and MEG2 to STAT3 regulates IL-6-mediated
            microglial hyperalgesia during inflammatory pain
  JOURNAL   FASEB J 38 (8), e23590 (2024)
   PUBMED   38656553
  REMARK    GeneRIF: The binding of PKCepsilon and MEG2 to STAT3 regulates
            IL-6-mediated microglial hyperalgesia during inflammatory pain.
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Xu,Y., Tao,J., Hou,Y. and Zhang,S.
  TITLE     Effects of PKCepsilon knockdown on mitochondrial membrane potential
            of human glioma cells in vitro and growth of U251 cell-derived
            tumors in vivo
  JOURNAL   Biotech Histochem 98 (7), 501-507 (2023)
   PUBMED   37501577
  REMARK    GeneRIF: Effects of PKCepsilon knockdown on mitochondrial membrane
            potential of human glioma cells in vitro and growth of U251
            cell-derived tumors in vivo.
REFERENCE   3  (bases 1 to 5749)
  AUTHORS   Khan,K., Zafar,S., Badshah,Y., Ashraf,N.M., Rafiq,M., Danish,L.,
            Shabbir,M., Trembley,J.H., Afsar,T., Almajwal,A. and Razak,S.
  TITLE     Cross talk of tumor protein D52 (TPD52) with KLF9, PKCepsilon, and
            MicroRNA 223 in ovarian cancer
  JOURNAL   J Ovarian Res 16 (1), 202 (2023)
   PUBMED   37833790
  REMARK    GeneRIF: Cross talk of tumor protein D52 (TPD52) with KLF9,
            PKCepsilon, and MicroRNA 223 in ovarian cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5749)
  AUTHORS   Piccarducci,R., Giacomelli,C., Bertilacchi,M.S.,
            Benito-Martinez,A., Di Giorgi,N., Daniele,S., Signore,G.,
            Rocchiccioli,S., Vilar,M., Marchetti,L. and Martini,C.
  TITLE     Apolipoprotein E epsilon4 triggers neurotoxicity via cholesterol
            accumulation, acetylcholine dyshomeostasis, and PKCepsilon
            mislocalization in cholinergic neuronal cells
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (7), 166793 (2023)
   PUBMED   37336366
  REMARK    GeneRIF: Apolipoprotein E epsilon4 triggers neurotoxicity via
            cholesterol accumulation, acetylcholine dyshomeostasis, and
            PKCepsilon mislocalization in cholinergic neuronal cells.
REFERENCE   5  (bases 1 to 5749)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   6  (bases 1 to 5749)
  AUTHORS   Ku,N.O. and Omary,M.B.
  TITLE     Identification of the major physiologic phosphorylation site of
            human keratin 18: potential kinases and a role in filament
            reorganization
  JOURNAL   J Cell Biol 127 (1), 161-171 (1994)
   PUBMED   7523419
REFERENCE   7  (bases 1 to 5749)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   8  (bases 1 to 5749)
  AUTHORS   Basta,P., Strickland,M.B., Holmes,W., Loomis,C.R., Ballas,L.M. and
            Burns,D.J.
  TITLE     Sequence and expression of human protein kinase C-epsilon
  JOURNAL   Biochim Biophys Acta 1132 (2), 154-160 (1992)
   PUBMED   1382605
REFERENCE   9  (bases 1 to 5749)
  AUTHORS   Omary,M.B., Baxter,G.T., Chou,C.F., Riopel,C.L., Lin,W.Y. and
            Strulovici,B.
  TITLE     PKC epsilon-related kinase associates with and phosphorylates
            cytokeratin 8 and 18
  JOURNAL   J Cell Biol 117 (3), 583-593 (1992)
   PUBMED   1374067
REFERENCE   10 (bases 1 to 5749)
  AUTHORS   Jakobovits,A., Rosenthal,A. and Capon,D.J.
  TITLE     Trans-activation of HIV-1 LTR-directed gene expression by tat
            requires protein kinase C
  JOURNAL   EMBO J 9 (4), 1165-1170 (1990)
   PUBMED   2182321
  REMARK    Erratum:[EMBO J 1990 Oct;9(10):3413]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U51244.1, AL832345.1, X65293.1,
            BC054052.1 and CA424727.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005400.2.
            
            Summary: Protein kinase C (PKC) is a family of serine- and
            threonine-specific protein kinases that can be activated by calcium
            and the second messenger diacylglycerol. PKC family members
            phosphorylate a wide variety of protein targets and are known to be
            involved in diverse cellular signaling pathways. PKC family members
            also serve as major receptors for phorbol esters, a class of tumor
            promoters. Each member of the PKC family has a specific expression
            profile and is believed to play a distinct role in cells. The
            protein encoded by this gene is one of the PKC family members. This
            kinase has been shown to be involved in many different cellular
            functions, such as neuron channel activation, apoptosis,
            cardioprotection from ischemia, heat shock response, as well as
            insulin exocytosis. Knockout studies in mice suggest that this
            kinase is important for lipopolysaccharide (LPS)-mediated signaling
            in activated macrophages and may also play a role in controlling
            anxiety-like behavior. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL832345.1, SRR11853567.16557.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306156.8/ ENSP00000306124.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               U51244.1           12804-13032
            230-1141            AL832345.1         131-1042
            1142-1997           X65293.1           731-1586
            1998-4737           AL832345.1         1899-4638
            4738-5486           BC054052.1         357-1105
            5487-5749           CA424727.1         18-280              c
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p21"
     gene            1..5749
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /note="protein kinase C epsilon"
                     /db_xref="GeneID:5581"
                     /db_xref="HGNC:HGNC:9401"
                     /db_xref="MIM:176975"
     exon            1..774
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..2640
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /EC_number="2.7.11.13"
                     /codon_start=1
                     /product="protein kinase C epsilon type"
                     /protein_id="NP_005391.1"
                     /db_xref="CCDS:CCDS1824.1"
                     /db_xref="GeneID:5581"
                     /db_xref="HGNC:HGNC:9401"
                     /db_xref="MIM:176975"
                     /translation="MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIAL
                     NVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQF
                     EELLQNGSRHFEDWIDLEPEGRVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAV
                     RRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIIT
                     KCAGLKKQETPDQVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVC
                     KMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLIAGAESPQPAS
                     GSSPSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLMSPGEN
                     GEVRQGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDV
                     DCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPR
                     SRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTT
                     FCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDV
                     LYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQKKIK
                     PPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP"
     exon            775..838
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            839..998
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1033
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1119
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1249
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1392
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1489
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1689
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1863
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..2018
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2157
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2346
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2493
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..5749
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5724..5729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /note="hexamer: AATAAA"
     polyA_site      5749
                     /gene="PRKCE"
                     /gene_synonym="nPKC-epsilon; PKCE"
                     /note="major polyA site"
ORIGIN      
        1 gaaatttccc gggattatgt ttcggaaagg taggtaagcg ccgggcgcgg cgcccgcttt
       61 cccgcagtcc ggagccggag agccagcgag gcggcgaggc agcccccgcg gcttgcagcg
      121 gagccgacag ctcgtcttct cttctggagg tgcagctggt ggtcgggggg agagacttgc
      181 tccaaacacg gacatccccc agctctcccc cctccctgtt ttccgttagg aacccggcga
      241 ggaaatacat gcactggctg agaatcgccc gcgccagggc gcaacgccac aaggtgtagg
      301 gagtgtgcgg ggtggggcga aaggggaccc aagagtccct gtggctcgga gtgccgggcc
      361 gtcggttctt cattcctgcc ctcggggcag acggagtgac cccggccccc actccccgcc
      421 ccgaccatgg tagtgttcaa tggccttctt aagatcaaaa tctgcgaggc cgtgagcttg
      481 aagcccacag cctggtcgct gcgccatgcg gtgggacccc ggccgcagac tttccttctc
      541 gacccctaca ttgccctcaa tgtggacgac tcgcgcatcg gccaaacggc caccaagcag
      601 aagaccaaca gcccggcctg gcacgacgag ttcgtcaccg atgtgtgcaa cggacgcaag
      661 atcgagctgg ctgtctttca cgatgccccc ataggctacg acgacttcgt ggccaactgc
      721 accatccagt ttgaggagct gctgcagaac gggagccgcc acttcgagga ctggattgat
      781 ctggagccag aaggaagagt gtatgtgatc atcgatctct cagggtcgtc gggtgaagcc
      841 cctaaagaca atgaagagcg tgtgttcagg gaacgcatgc ggccgaggaa gcggcagggg
      901 gccgtcaggc gcagggtcca tcaggtcaac ggccacaagt tcatggccac ctatcttcgg
      961 cagcccacct actgctccca ttgcagagac ttcatctggg gtgtcatagg aaagcaggga
     1021 taccagtgtc aagtctgcac ctgcgtggtc cacaagcggt gccacgagct cataatcaca
     1081 aagtgtgctg ggttaaagaa gcaggagacc cccgaccagg tgggctccca gcggttcagc
     1141 gtcaacatgc cccacaagtt cggtatccac aactacaagg tccctacctt ctgcgatcac
     1201 tgtgggtccc tgctctgggg actcttgcgg cagggtttgc agtgtaaagt ctgcaaaatg
     1261 aatgttcacc gtcgatgtga gaccaacgtg gctcccaact gtggagtgga tgccagagga
     1321 atcgccaaag tactggccga cctgggcgtt accccagaca aaatcaccaa cagcggccag
     1381 agaaggaaaa agctcattgc tggtgccgag tccccgcagc ctgcttctgg aagctcacca
     1441 tctgaggaag atcgatccaa gtcagcaccc acctcccctt gtgaccagga aataaaagaa
     1501 cttgagaaca acattcggaa agccttgtca tttgacaacc gaggagagga gcaccgggca
     1561 gcatcgtctc ctgatggcca gctgatgagc cccggtgaga atggcgaagt ccggcaaggc
     1621 caggccaagc gcctgggcct ggatgagttc aacttcatca aggtgttggg caaaggcagc
     1681 tttggcaagg tcatgttggc agaactcaag ggcaaagatg aagtatatgc tgtgaaggtc
     1741 ttaaagaagg acgtcatcct tcaggatgat gacgtggact gcacaatgac agagaagagg
     1801 attttggctc tggcacggaa acacccgtac cttacccaac tctactgctg cttccagacc
     1861 aaggaccgcc tctttttcgt catggaatat gtaaatggtg gagacctcat gtttcagatt
     1921 cagcgctccc gaaaattcga cgagcctcgt tcacggttct atgctgcaga ggtcacatcg
     1981 gccctcatgt tcctccacca gcatggagtc atctacaggg atttgaaact ggacaacatc
     2041 cttctggatg cagaaggtca ctgcaagctg gctgacttcg ggatgtgcaa ggaagggatt
     2101 ctgaatggtg tgacgaccac cacgttctgt gggactcctg actacatagc tcctgagatc
     2161 ctgcaggagt tggagtatgg cccctccgtg gactggtggg ccctgggggt gctgatgtac
     2221 gagatgatgg ctggacagcc tccctttgag gccgacaatg aggacgacct atttgagtcc
     2281 atcctccatg acgacgtgct gtacccagtc tggctcagca aggaggctgt cagcatcttg
     2341 aaagctttca tgacgaagaa tccccacaag cgcctgggct gtgtggcatc gcagaatggc
     2401 gaggacgcca tcaagcagca cccattcttc aaagagattg actgggtgct cctggagcag
     2461 aagaagatca agccaccctt caaaccacgc attaaaacca aaagagacgt caataatttt
     2521 gaccaagact ttacccggga agagccggta ctcacccttg tggacgaagc aattgtaaag
     2581 cagatcaacc aggaggaatt caaaggtttc tcctactttg gtgaagacct gatgccctga
     2641 gagcccactg cagttggact ttgccgatgc tgcaagaagg ggtgcagaga agactcctgt
     2701 gttggagaca ctcagcaggt cttgaactac ttctcctcct cggagcccca gtcccatgtc
     2761 cactgtctat ttattgcatt cccttgcccc aggccacctc ctccccctcc cacctggtga
     2821 ccagaaggcg ctctcggttc ttgtctcacc agtaatgcag actcattggg tcagcaatta
     2881 gctgtataca ctgccgtgtt tggaccattg gcaagcctgg ttccactcct caggggctcc
     2941 tggcagtgaa gcaacttcag ttcttttact gcaaagaaca gaaaaaagaa agaaagcaaa
     3001 caagaagact ccggctctgc tatcggacac agatcctgat ccctcttgct tcttttccct
     3061 cctgcaccgc agcttgccat ccctgccctt ctgtcctgga gaagagactg gtgcttctcc
     3121 gcacacacga gggagggcgc ccttgaggca tgccctctga gggagggaga ccagagatgc
     3181 agggattggc cagctgggtt ggtttgctct ggaatggcta actcttgcct gctttggttt
     3241 tagcttttca gcatgccaaa gtcatgtaag tttgtgtctt gtggaagaaa tcctctttgt
     3301 ggaaaaagaa acagggtttt gaactctgtt aacatttgaa aaatatattt tcaaattcac
     3361 tttctaattg gccaaaagag atgagttcca gtctgaatac aggtagatat taaagggcta
     3421 ataaaaaatg agaaaccggt cgtccaaggt ggatgctgtc aatgcccgag tgacacatga
     3481 gagctgtatg aattgagaga aaaggcaaca agtagcattc ttcatcattc aagttctacc
     3541 tggacacaaa ggcgaggacc ctggggttcc aacaaagctc agctcccaga ttctctttcc
     3601 agtttcatcc taagttccta gcataaacac tatttatttt ctgcagcagt gtgttatttt
     3661 tgcgcactta tacaaaatgg tagtactact gtgttgtggt ttttaaacat taaacatgta
     3721 aagttatata cgaaatatct gcttttggaa taagcagaat gaggctaaac atgggttata
     3781 caaagggtat ctggaaactg aagagcaact tgttagaaaa ctgacaatgt cgcaagatgt
     3841 actcagtttt gtttctgtgt gacatgcaat ggcaactcat gtggacacta ttgaagggat
     3901 gtgacattac ctcctgtaga tatgctaaca gtgttattct ttcatttcca agggttctct
     3961 gtggctttgt gtatatgttt cccagaggtc atttgattac ctaatttact gaactgattt
     4021 agcagggaat ggaatccatt ccaactattg cacgtggatt tcccagctgc ccctaaatat
     4081 atatacttgt gagtggcaaa gtggcactaa tgaagctttt gccttttgta catttgagat
     4141 ttttgtatat agtgtttgct gcaaggcctg tggaattaat tcgttgcata tagaggtatc
     4201 aactgctgca tgttcaggca tattataaaa ctttagtcta tgaaagaata attataataa
     4261 tgtccaggtg caatactctg taagtctatt ggttcaagtt accgagagat aggtgtgttc
     4321 ctttatgggg gatggggggg tgtgttgggg attctttgta ttgtttattt cattttggtt
     4381 tattttaaaa gatgtaaaca tatattaagc tatattaaat ctcacataca gttcttctgt
     4441 gctctattat accctgatag agatggggga gagaaaggaa tgtttttgat ggtggtttca
     4501 aagctcggac agtaactatc ttgagcccat tagagagtct gtgtccatat ttgcatctgg
     4561 ctggtcatag cctttgttac taatgatgac attcagttct cttttgtttt tattttttaa
     4621 aaactcaggt gtaattatta tctgttctta agataattgc aaatattaaa tattatgata
     4681 tatcaattca tgtgtttggc ataccagtga atgatgaaga acatgagatt aatttaattt
     4741 atcttcggta acttgacatt ctggagagag actatcttct ggagttgagt acaagcacag
     4801 aaacatcttt acggtggcat catctcattt tttaggaaga catgataata ctgcccatca
     4861 tattcatgtg taactactgt tctttcttct gctttcttca ccataataaa ctttggacaa
     4921 ccaagcaagc tctaaccgca atgccagatg gccttgtccg agggcctagt gtttgcacgg
     4981 cagtgggaac tgggcctttc ctacaggaca actggcaagt ttgctgggaa gtcaaataat
     5041 acattccacc tggcagctga aggcagccag tcagtctgtc ccagaaaggg cccttttcag
     5101 cacccaaagc tgggctggct gggatgcctc tggctggtga agttctcaca taggctgatt
     5161 taaatccagc aaaggtctat agaaaaaggc ttgcgtgttc gttgagtaat cattgtttca
     5221 ttttcatttt tacgagagtt tgaaaataga cacactgtta acacttctgc cagttttttc
     5281 tgatctttcc agccccaccc cctttctctt tctctctctc tctcaaagaa aaaaaaaatg
     5341 ggagtgcaaa aaaaacaaag ccaaaaaata tatgaaggat agctgttctt ctgtgttctc
     5401 tcattatgga ctttgtgaag tagaaacata attttttttc ctccaaaggt gaaaaaacaa
     5461 tgcattcttg ctttaaaaaa aaaaaagaag gctaaaaaat tacctctttt taaattatgt
     5521 gcaaaataat tctggctaac tgtaaaatgt attcaatttt aggatttttt ttttttgtat
     5581 tgtgatgctt tatttgtaca tttttttcct ttctggatgt aattttaatc tcttgccatt
     5641 cattagtgtt atttcattgt aaacgttatt gtgccaaatg tactgtattc aaaaggatgt
     5701 gaatgtgtat tgtttcagaa cctaataaat acaatgacgt taagtctta
//
