LOCUS       NM_002738               8010 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens protein kinase C beta (PRKCB), transcript variant 2,
            mRNA.
ACCESSION   NM_002738
VERSION     NM_002738.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Nieto-Felipe,J., Sanchez-Collado,J., Jardin,I., Salido,G.M.,
            Lopez,J.J. and Rosado,J.A.
  TITLE     The store-operated Ca2+ channel Orai1alpha is required for
            agonist-evoked NF-kappaB activation by a mechanism dependent on
            PKCbeta2
  JOURNAL   J Biol Chem 299 (2), 102882 (2023)
   PUBMED   36623731
  REMARK    GeneRIF: The store-operated Ca[2+] channel Orai1alpha is required
            for agonist-evoked NF-kappaB activation by a mechanism dependent on
            PKCbeta2.
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Iwanicki,T., Balcerzyk,A., Kazek,B., Emich-Widera,E., Likus,W.,
            Iwanicka,J., Kapinos-Gorczyca,A., Kapinos,M., Jarosz,A.,
            Grzeszczak,W., Gorczynska-Kosiorz,S. and Niemiec,P.
  TITLE     Family-Based Cohort Association Study of PRKCB1, CBLN1 and KCNMB4
            Gene Polymorphisms and Autism in Polish Population
  JOURNAL   J Autism Dev Disord 52 (10), 4213-4218 (2022)
   PUBMED   34562210
  REMARK    GeneRIF: Family-Based Cohort Association Study of PRKCB1, CBLN1 and
            KCNMB4 Gene Polymorphisms and Autism in Polish Population.
REFERENCE   3  (bases 1 to 8010)
  AUTHORS   Renkhold,L., Kollmann,R., Inderwiedenstrasse,L. and Kienitz,M.C.
  TITLE     PKC-isoform specific regulation of receptor desensitization and
            KCNQ1/KCNE1 K+ channel activity by mutant alpha1B-adrenergic
            receptors
  JOURNAL   Cell Signal 91, 110228 (2022)
   PUBMED   34958868
  REMARK    GeneRIF: PKC-isoform specific regulation of receptor
            desensitization and KCNQ1/KCNE1 K(+) channel activity by mutant
            alpha1B-adrenergic receptors.
REFERENCE   4  (bases 1 to 8010)
  AUTHORS   Zhang,H.L., Hu,B.X., Li,Z.L., Du,T., Shan,J.L., Ye,Z.P., Peng,X.D.,
            Li,X., Huang,Y., Zhu,X.Y., Chen,Y.H., Feng,G.K., Yang,D., Deng,R.
            and Zhu,X.F.
  TITLE     PKCbetaII phosphorylates ACSL4 to amplify lipid peroxidation to
            induce ferroptosis
  JOURNAL   Nat Cell Biol 24 (1), 88-98 (2022)
   PUBMED   35027735
  REMARK    GeneRIF: PKCbetaII phosphorylates ACSL4 to amplify lipid
            peroxidation to induce ferroptosis.
REFERENCE   5  (bases 1 to 8010)
  AUTHORS   Kadir,R.R.A., Alwjwaj,M., McCarthy,Z. and Bayraktutan,U.
  TITLE     Therapeutic hypothermia augments the restorative effects of
            PKC-beta and Nox2 inhibition on an in vitro model of human
            blood-brain barrier
  JOURNAL   Metab Brain Dis 36 (7), 1817-1832 (2021)
   PUBMED   34398388
  REMARK    GeneRIF: Therapeutic hypothermia augments the restorative effects
            of PKC-beta and Nox2 inhibition on an in vitro model of human
            blood-brain barrier.
REFERENCE   6  (bases 1 to 8010)
  AUTHORS   Obeid,L.M., Blobe,G.C., Karolak,L.A. and Hannun,Y.A.
  TITLE     Cloning and characterization of the major promoter of the human
            protein kinase C beta gene. Regulation by phorbol esters
  JOURNAL   J Biol Chem 267 (29), 20804-20810 (1992)
   PUBMED   1400396
REFERENCE   7  (bases 1 to 8010)
  AUTHORS   Goode,N., Hughes,K., Woodgett,J.R. and Parker,P.J.
  TITLE     Differential regulation of glycogen synthase kinase-3 beta by
            protein kinase C isotypes
  JOURNAL   J Biol Chem 267 (24), 16878-16882 (1992)
   PUBMED   1324914
REFERENCE   8  (bases 1 to 8010)
  AUTHORS   Moss,S.J., Doherty,C.A. and Huganir,R.L.
  TITLE     Identification of the cAMP-dependent protein kinase and protein
            kinase C phosphorylation sites within the major intracellular
            domains of the beta 1, gamma 2S, and gamma 2L subunits of the
            gamma-aminobutyric acid type A receptor
  JOURNAL   J Biol Chem 267 (20), 14470-14476 (1992)
   PUBMED   1321150
REFERENCE   9  (bases 1 to 8010)
  AUTHORS   Devalia,V., Thomas,N.S., Roberts,P.J., Jones,H.M. and Linch,D.C.
  TITLE     Down-regulation of human protein kinase C alpha is associated with
            terminal neutrophil differentiation
  JOURNAL   Blood 80 (1), 68-76 (1992)
   PUBMED   1611098
REFERENCE   10 (bases 1 to 8010)
  AUTHORS   Niino,Y.S., Ohno,S. and Suzuki,K.
  TITLE     Positive and negative regulation of the transcription of the human
            protein kinase C beta gene
  JOURNAL   J Biol Chem 267 (9), 6158-6163 (1992)
   PUBMED   1556124
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB456157.1, BC036472.1 and
            AC130448.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_002738.6.
            
            Summary: Protein kinase C (PKC) is a family of serine- and
            threonine-specific protein kinases that can be activated by calcium
            and second messenger diacylglycerol. PKC family members
            phosphorylate a wide variety of protein targets and are known to be
            involved in diverse cellular signaling pathways. PKC family members
            also serve as major receptors for phorbol esters, a class of tumor
            promoters. Each member of the PKC family has a specific expression
            profile and is believed to play a distinct role in cells. The
            protein encoded by this gene is one of the PKC family members. This
            protein kinase has been reported to be involved in many different
            cellular functions, such as B cell activation, apoptosis induction,
            endothelial cell proliferation, and intestinal sugar absorption.
            Studies in mice also suggest that this kinase may also regulate
            neuronal functions and correlate fear-induced conflict behavior
            after stress. Alternatively spliced transcript variants encoding
            distinct isoforms have been reported. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.243143.1,
                                           SRR1803617.171461.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000643927.1/ ENSP00000496129.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DB456157.1         5-45
            42-2232             BC036472.1         6-2196
            2233-8010           AC130448.2         28957-34734         c
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2-p12.1"
     gene            1..8010
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /note="protein kinase C beta"
                     /db_xref="GeneID:5579"
                     /db_xref="HGNC:HGNC:9395"
                     /db_xref="MIM:176970"
     exon            1..366
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..2215
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /EC_number="2.7.11.13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein kinase C, beta 1 polypeptide; protein kinase C
                     beta type; PKC-B"
                     /codon_start=1
                     /product="protein kinase C beta type isoform 2"
                     /protein_id="NP_002729.2"
                     /db_xref="CCDS:CCDS10619.1"
                     /db_xref="GeneID:5579"
                     /db_xref="HGNC:HGNC:9395"
                     /db_xref="MIM:176970"
                     /translation="MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFF
                     KQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKH
                     KFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRG
                     RIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCS
                     LNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFK
                     LLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNR
                     DRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKR
                     VLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEI
                     AIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYI
                     APEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSK
                     EAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGR
                     NAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS"
     exon            367..398
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            399..481
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            482..593
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            594..722
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..879
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1014
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1111
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1258
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1432
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1524
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1587
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1726
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1807
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1915
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2056
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..8010
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7987..7992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /note="hexamer: ATTAAA"
     polyA_site      8010
                     /gene="PRKCB"
                     /gene_synonym="PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1;
                     PRKCB2"
                     /note="major polyA site"
ORIGIN      
        1 ggacgagcgg cagcagctgg gcgagtgaca gccccggctc cgcgcgccgc ggccgccaga
       61 gccggcgcag gggaagcgcc cgcggccccg ggtgcagcag cggccgccgc ctcccgcgcc
      121 tccccggccc gcagcccgcg gtcccgcggc cccggggccg gcacctctcg ggctccggct
      181 ccccgcgcgc aagatggctg acccggctgc ggggccgccg ccgagcgagg gcgaggagag
      241 caccgtgcgc ttcgcccgca aaggcgccct ccggcagaag aacgtgcatg aggtcaagaa
      301 ccacaaattc accgcccgct tcttcaagca gcccaccttc tgcagccact gcaccgactt
      361 catctggggc ttcgggaagc agggattcca gtgccaagtt tgctgctttg tggtgcacaa
      421 gcggtgccat gaatttgtca cattctcctg ccctggcgct gacaagggtc cagcctccga
      481 tgacccccgc agcaaacaca agtttaagat ccacacgtac tccagcccca cgttttgtga
      541 ccactgtggg tcactgctgt atggactcat ccaccagggg atgaaatgtg acacctgcat
      601 gatgaatgtg cacaagcgct gcgtgatgaa tgttcccagc ctgtgtggca cggaccacac
      661 ggagcgccgc ggccgcatct acatccaggc ccacatcgac agggacgtcc tcattgtcct
      721 cgtaagagat gctaaaaacc ttgtacctat ggaccccaat ggcctgtcag atccctacgt
      781 aaaactgaaa ctgattcccg atcccaaaag tgagagcaaa cagaagacca aaaccatcaa
      841 atgctccctc aaccctgagt ggaatgagac atttagattt cagctgaaag aatcggacaa
      901 agacagaaga ctgtcagtag agatttggga ttgggatttg accagcagga atgacttcat
      961 gggatctttg tcctttggga tttctgaact tcagaaagcc agtgttgatg gctggtttaa
     1021 gttactgagc caggaggaag gcgagtactt caatgtgcct gtgccaccag aaggaagtga
     1081 ggccaatgaa gaactgcggc agaaatttga gagggccaag atcagtcagg gaaccaaggt
     1141 cccggaagaa aagacgacca acactgtctc caaatttgac aacaatggca acagagaccg
     1201 gatgaaactg accgatttta acttcctaat ggtgctgggg aaaggcagct ttggcaaggt
     1261 catgctttca gaacgaaaag gcacagatga gctctatgct gtgaagatcc tgaagaagga
     1321 cgttgtgatc caagatgatg acgtggagtg cactatggtg gagaagcggg tgttggccct
     1381 gcctgggaag ccgcccttcc tgacccagct ccactcctgc ttccagacca tggaccgcct
     1441 gtactttgtg atggagtacg tgaatggggg cgacctcatg tatcacatcc agcaagtcgg
     1501 ccggttcaag gagccccatg ctgtatttta cgctgcagaa attgccatcg gtctgttctt
     1561 cttacagagt aagggcatca tttaccgtga cctaaaactt gacaacgtga tgctcgattc
     1621 tgagggacac atcaagattg ccgattttgg catgtgtaag gaaaacatct gggatggggt
     1681 gacaaccaag acattctgtg gcactccaga ctacatcgcc cccgagataa ttgcttatca
     1741 gccctatggg aagtccgtgg attggtgggc atttggagtc ctgctgtatg aaatgttggc
     1801 tgggcaggca ccctttgaag gggaggatga agatgaactc ttccaatcca tcatggaaca
     1861 caacgtagcc tatcccaagt ctatgtccaa ggaagctgtg gccatctgca aagggctgat
     1921 gaccaaacac ccaggcaaac gtctgggttg tggacctgaa ggcgaacgtg atatcaaaga
     1981 gcatgcattt ttccggtata ttgattggga gaaacttgaa cgcaaagaga tccagccccc
     2041 ttataagcca aaagcttgtg ggcgaaatgc tgaaaacttc gaccgatttt tcacccgcca
     2101 tccaccagtc ctaacacctc ccgaccagga agtcatcagg aatattgacc aatcagaatt
     2161 cgaaggattt tcctttgtta actctgaatt tttaaaaccc gaagtcaaga gctaagtaga
     2221 tgtgtagatc tccgtccttc atttctgtca ttcaagctca acggctattg tggtgacatt
     2281 tttatgtttt tcattgccaa gttgcatcca tgtttgattt tctgatgaga ctagagtgac
     2341 agtgtttcag aacccaaatg tcctcaggta gtttggagca tctctatgag atgggattat
     2401 gcagatggcc tatggaaaat gcagctgcat aattaacaca ttatcaaagt cctcttacaa
     2461 tttattttcc gcagcatgtc agctaagtag acccaatggg gagagaaaat gcctgctttc
     2521 tttccctctt tttctgcact gccatattca cccccaacca tccaatctgt ggataattgg
     2581 atgttagcgg tactcttcca cttccgggcc tggagcttgg cttgtatcca agtgtatggt
     2641 tgctttgcct aagaggaatc cctctatttc acctgttctg gaggcaccag accttgaaaa
     2701 gaacatgctc aaaataaaat gttatctgtt atttttgtaa actcaaagtt aagatgatca
     2761 aagttctaaa attccaagaa tgtgctttta gacggtctca atctaaaagc acttcaaggg
     2821 gtcaaagggc aaccagcttg ggtgctacct cagtgttgta gtttctgata ctttatgtct
     2881 ttgctcaccc tcatccccaa actacttgaa aagggcattt ggcaccactc tctgaaacaa
     2941 cacagtcact ctagcaaggc ccccaaaggg ccctggtttt acattacatt tcaaacttta
     3001 tttgctttgg ggttttgttt ctgttgttgt tcaaatgcaa aaaaaagaaa aaaaaagaaa
     3061 aaaaaaggtg actcacattg ttacacatgc tttaaaatat gtattcaaat gttattaacc
     3121 acaatgacga cctgctttga tttaaccaag aagacggctg cggagcctag cagactcagg
     3181 cctgtgggaa tgggatttgt tacaaatcta ggtttgttac tggcttcaga aagctaatta
     3241 agtgctctga aaaagacacc gtttcttgaa acaaagatgg ttgtattcct cactttgatg
     3301 ttgttttgca agatgtttgt ggaaatgttc atttgtatct ggatctctgt tatgtgccat
     3361 ttttcttcta gcatcgagat acaataaaaa aaaaaaaaaa gaaaagaaga agaaatacta
     3421 tttcaaggaa aactgctctt tttgagaaac gtggacctaa actacaaagt gggaactgag
     3481 gagggaactc aggagaaagg aactaactgc ggagctttaa tcttggcccc agtgttcagc
     3541 cactcggagg ggcgggggct gtggcccatt caggggctgc tggtgggctg tagtggggtg
     3601 ggatgacctg gccagagcca acgaggatac tggagcccaa agtcaagttt agagaccagc
     3661 tgggaacgtg aatggggctc ttgattttct tatcaaaatc accactcctc ccagcttgga
     3721 ctaaatattc tttctagcaa gcagctttgt gagctccctg aagcccaagg aaacccttcg
     3781 gtgggagaaa tttcatttct gtctgagagg attaaggcag caggtgactc cccctcctcg
     3841 cctgccgtgt cctgctattc tcaggcagct ctaaggagaa ttcttatcac agttcaagtg
     3901 atttccagaa gttccagggc ttctgagaga ccatcaaggg aactttaaca acttgacaaa
     3961 tgtccttgaa gtaagatgcc tcatctttag ggaaaaatgg ggtttggatt tctgcttagg
     4021 caaagtctcc tgcagttcat ccttctctgt cctcttcttg cttcaggctt ggggaccgtc
     4081 cctgctgtcc ccactgtggt ggcaatcagg acctaaggtg aagcaaactt gaagttctat
     4141 ctgacaagtt taggcagtaa gagaaggagg gaaatcggag caaagctccc tcactttatt
     4201 gttgagaaac tggcatctgg aaagaggaag gaatttgccc aaagtcagtc agctgggata
     4261 aaaacctggg tgtcctgtcc agaaagtgca gggtgctttc tgctctgtag caaggcagca
     4321 gacatctctg agccaggccc accaacaggc ccttatctgg tggttggatc atgatcccat
     4381 tttgcttgga catgctctca ggaagataaa aaccatggag aaacactagg ccattgacaa
     4441 atgatctgag acaactttag aaaacaatgt aggatgaatg gaaagagaaa gaaaggaaag
     4501 aaagaagaaa aagaaagaag gaaagaaaga aagagaaagg aaggaaggaa agaaggaagg
     4561 aaaagaagga aggaaggaag gaatatagtg ttataaatac tgcactcaac attttccaaa
     4621 ttcttgccat tatttttcaa aagtttaata gtttgcagaa atagatactc aagccaaagt
     4681 ctgttttaga gaaactttcc atggaaagtc agaatttcta ccacttcctt ttctatccac
     4741 atttccagtg cagaagaaac tgagaaacag agctttttga agagaggaca gggccatagc
     4801 aacaaggacc ttcttggggg attaatggga ggtcagtaga attaataacc ctccttggat
     4861 gagtgctact gttttcacat ggcttcagat gctatcaacc tcaaagaaat gatctcaaca
     4921 gagaagctta ttctctccca acttctacgg taaaatccag gagtattttc tctggggatc
     4981 tgcccacagg acaaagtcca taaaagcaag tcctgtctgg accatgtggt tatctgaagc
     5041 attagccatc accagcacaa caaacggggc agggctttcc aaggtggggc tggtcagaag
     5101 ggaatctttg ataagaggcc cacaggcagg gaaagcgaaa tagggttgat gagaccaggg
     5161 gagacctaaa aaaaaggcag ctttgtgtct tctagctcca aatatacctg ccttttagct
     5221 cacacactgt cctggagttc tcagaccttt aggggcccta acacagttca gttcatacag
     5281 gggttcaaaa gggacagtgg cccatttggg agacctttag gatcaatggg aatcaattcc
     5341 attgttttgc ctcagagtaa agtttctggc tcggggacaa ttataagttg caaaaaggat
     5401 agaggcatat cccaagtctt ccttcattcc acaaataatt acaaacaacc tactgtgtgc
     5461 caggcactat tcttagcact ggaaatacac tagtgaagaa gcagatgagg accctgttta
     5521 ttgtttctct ccaagaaatt ctccaagaat attgtttctt ggagagaaat aataaataaa
     5581 caagacaatt tctgaaagca ataagtgcaa tcaagataat taaaggatgc taaagtgtga
     5641 cttgtgggga ttgggagaga gatgcacaga caatattaaa gaggaggcat tcgagctttg
     5701 ttgtgaacac cggaagtaac atgccgagcg cctgggggat ggaaactcct atagcacccc
     5761 acaggctaac agcaagcagg acaagacaaa aagggcaggt gggacatggt agagatggac
     5821 cctacccagg aaacagctcc atcagcatct tagcctgccc cactctagcc acacataccc
     5881 acgtgtgctc ctgagttcag tgtgcccacc tcactcccac accctcacat agacttggca
     5941 agagtaagga gggaactcca tagagacatt ttacctatct caggggagca gccacaaaga
     6001 agcaagtctt gtaaaaggtc ttttgcaaag gagagtgaac ccagcaatga gagatcctta
     6061 acagctagtg cccattaggg ggctaaacct aaagcctggg tggtgatggc tcaaacgcta
     6121 atgagtcagt gaatccttac cgaccccctg gcctttataa tctgaggcaa ctttggctgc
     6181 agcccgggaa tgtgcagggc actagggaat acaaggcctt cttccctggt tgtcttgtaa
     6241 taaaacagcc atggggttgt ccctccagtc cgagagactg tgatgaggcc tacatagcag
     6301 cgatgtggtc aggtaaaaat caggaaccca ctgaaatctt gggcaagcca ccctgcctgc
     6361 ttgtgcctcg gttctctcat atgtcatata taggaggtga ggactccagc tccacctgcc
     6421 ccaggtgggt gtggtgatga tgaggaaaga caagaggctt gcaaggaccc tgaagaggtc
     6481 ggagcatcat acagattcct ttattagccc acattctgat gttccctggt gagacttgcc
     6541 ccaagcaatt gctagtaaat gggggttaat ttcttctcca cctccctact gaacaaaaaa
     6601 agaaatgcca gacttactag gagaatcgag ttgctttgag tttcttttgt tttgttttgt
     6661 tttgttttgt tttaaggctc cccttacaca ccctccttta agctttgggt tttctctctt
     6721 atagtttgtt gacacatgct aaaaatgtct ttggagagaa cttctgcctg ataaacaccc
     6781 aattctagac tgtgggtgga ttttcgagct gacggtggtc aattcctttc attaagcagt
     6841 gatctgattt ctccacatgg ccattctgcc ttcttggggg cagagtagat gggcagcagt
     6901 tcaccttttc agagaaagag gtcttctagc cacctgggct gctactgaat ggttttctcc
     6961 aggacgctct acctaatgat tatttctata acattaagca tggtaataag tagcttccaa
     7021 ttcaattcat cctaaagcca aagaaaatac agcaacacac acacacacac acacacacac
     7081 acacacacac acacacacac accactttat ggcaattctt aactgacatt caatgactta
     7141 cttcttttct tagaaaattt ccaccacatt tctatcccca agccaacata caatgtgaaa
     7201 tgaaagccag tgcgtggagt gcagctgcta aaaattttca gcacagggct ctttctgact
     7261 ctgctcatga gatggtatca gccacccaat gactggcgta tcttggtcct gtgtctttct
     7321 tcttacgctg tgttaatgtg tttactttcc atttggcaga gagacaagag agacacctcc
     7381 aacttcgaca aagagttcac cagacagcct gtggaactga cccccactga taaactcttc
     7441 atcatgaact tggaccaaaa tgaatttgct ggcttctctt atactaaccc agagtttgtc
     7501 attaatgtgt aggtgaatgc aaactccatc gttgagcctg gggtgtaaga cttcaagcca
     7561 agcgtatgta tcaattctag tcttccagga ttcacggtgc acatgctggc attcaacatg
     7621 tggaaagctt gtcttagagg gcttttcttt gtatgtgtag cttgctagtt tgttttctac
     7681 atttgaaaat gtttagttta gaataagcgc attatccaat tatagaggta caattttcca
     7741 aacttccaga aactcatcaa atgaacagac aatgtcaaaa ctactgtgtc tgataccaaa
     7801 atgcttcagt atttgtaatt tttcaagtca gaagctgatg ttcctggtaa aagtttttac
     7861 agttattcta taatatcttc tttgaatgct aagcatgagc gatattttta aaaattgtga
     7921 gtaagctttg cagttactgt gaactattgt ctcttggagg aagttttttg tttaagaatt
     7981 gatatgatta aactgaatta atatatgcaa
//
