LOCUS       NM_002737               8964 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens protein kinase C alpha (PRKCA), mRNA.
ACCESSION   NM_002737
VERSION     NM_002737.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Xu,Y., Cohen,E., Johnson,C.N., Parent,C.A. and Coulombe,P.A.
  TITLE     Repeated stress to the skin amplifies neutrophil infiltration in a
            keratin 17- and PKCalpha-dependent manner
  JOURNAL   PLoS Biol 22 (8), e3002779 (2024)
   PUBMED   39159283
  REMARK    GeneRIF: Repeated stress to the skin amplifies neutrophil
            infiltration in a keratin 17- and PKCalpha-dependent manner.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Zhao,J., Tian,H., Zhao,X., Lan,L., Liu,H., Sun,Y. and Yu,F.
  TITLE     PKCalpha Induced the Generation of Extracellular Vesicles in
            Activated Platelets to Promote Breast Cancer Metastasis
  JOURNAL   Int J Biol Sci 20 (10), 3956-3971 (2024)
   PUBMED   39113702
  REMARK    GeneRIF: PKCalpha Induced the Generation of Extracellular Vesicles
            in Activated Platelets to Promote Breast Cancer Metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8964)
  AUTHORS   Gholam Azad,M., Hussaini,M., Russell,T.M., Richardson,V., Kaya,B.,
            Dharmasivam,M. and Richardson,D.R.
  TITLE     Multi-modal mechanisms of the metastasis suppressor, NDRG1:
            Inhibition of WNT/beta-catenin signaling by stabilization of
            protein kinase Calpha
  JOURNAL   J Biol Chem 300 (7), 107417 (2024)
   PUBMED   38815861
  REMARK    GeneRIF: Multi-modal mechanisms of the metastasis suppressor,
            NDRG1: Inhibition of WNT/beta-catenin signaling by stabilization of
            protein kinase Calpha.
REFERENCE   4  (bases 1 to 8964)
  AUTHORS   Pang,L., Cui,M., Wu,S. and Kong,J.
  TITLE     Cav-1 regulates the bile salt export pump on the canalicular
            membrane of hepatocytes by PKCalpha-associated signalling under
            cholesterol stimulation
  JOURNAL   J Cell Mol Med 28 (3), e18110 (2024)
   PUBMED   38164042
  REMARK    GeneRIF: Cav-1 regulates the bile salt export pump on the
            canalicular membrane of hepatocytes by PKCalpha-associated
            signalling under cholesterol stimulation.
REFERENCE   5  (bases 1 to 8964)
  AUTHORS   Chong,P.S.Y., Chooi,J.Y., Lim,J.S.L., Leow,A.C.Y., Toh,S.H.M.,
            Azaman,I., Koh,M.Y., Teoh,P.J., Tan,T.Z., Chung,T.H. and Chng,W.J.
  TITLE     Histone Methyltransferase NSD2 Activates PKCalpha to Drive
            Metabolic Reprogramming and Lenalidomide Resistance in Multiple
            Myeloma
  JOURNAL   Cancer Res 83 (20), 3414-3427 (2023)
   PUBMED   37463241
  REMARK    GeneRIF: Histone Methyltransferase NSD2 Activates PKCalpha to Drive
            Metabolic Reprogramming and Lenalidomide Resistance in Multiple
            Myeloma.
REFERENCE   6  (bases 1 to 8964)
  AUTHORS   Li,L., Zhou,J., James,G., Heller-Harrison,R., Czech,M.P. and
            Olson,E.N.
  TITLE     FGF inactivates myogenic helix-loop-helix proteins through
            phosphorylation of a conserved protein kinase C site in their
            DNA-binding domains
  JOURNAL   Cell 71 (7), 1181-1194 (1992)
   PUBMED   1335366
REFERENCE   7  (bases 1 to 8964)
  AUTHORS   Rider,M.H., Vandamme,J., Lebeau,E., Vertommen,D., Vidal,H.,
            Rousseau,G.G., Vandekerckhove,J. and Hue,L.
  TITLE     The two forms of bovine heart
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from
            alternative splicing
  JOURNAL   Biochem J 285 (Pt 2) (Pt 2), 405-411 (1992)
   PUBMED   1322130
REFERENCE   8  (bases 1 to 8964)
  AUTHORS   Moss,S.J., Doherty,C.A. and Huganir,R.L.
  TITLE     Identification of the cAMP-dependent protein kinase and protein
            kinase C phosphorylation sites within the major intracellular
            domains of the beta 1, gamma 2S, and gamma 2L subunits of the
            gamma-aminobutyric acid type A receptor
  JOURNAL   J Biol Chem 267 (20), 14470-14476 (1992)
   PUBMED   1321150
REFERENCE   9  (bases 1 to 8964)
  AUTHORS   Bredt,D.S., Ferris,C.D. and Snyder,S.H.
  TITLE     Nitric oxide synthase regulatory sites. Phosphorylation by cyclic
            AMP-dependent protein kinase, protein kinase C, and
            calcium/calmodulin protein kinase; identification of flavin and
            calmodulin binding sites
  JOURNAL   J Biol Chem 267 (16), 10976-10981 (1992)
   PUBMED   1375933
REFERENCE   10 (bases 1 to 8964)
  AUTHORS   Omary,M.B., Baxter,G.T., Chou,C.F., Riopel,C.L., Lin,W.Y. and
            Strulovici,B.
  TITLE     PKC epsilon-related kinase associates with and phosphorylates
            cytokeratin 8 and 18
  JOURNAL   J Cell Biol 117 (3), 583-593 (1992)
   PUBMED   1374067
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009452.21, AC006263.1,
            AC006947.2, AC005918.13 and AC005988.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002737.2.
            
            Summary: Protein kinase C (PKC) is a family of serine- and
            threonine-specific protein kinases that can be activated by calcium
            and the second messenger diacylglycerol. PKC family members
            phosphorylate a wide variety of protein targets and are known to be
            involved in diverse cellular signaling pathways. PKC family members
            also serve as major receptors for phorbol esters, a class of tumor
            promoters. Each member of the PKC family has a specific expression
            profile and is believed to play a distinct role in cells. The
            protein encoded by this gene is one of the PKC family members. This
            kinase has been reported to play roles in many different cellular
            processes, such as cell adhesion, cell transformation, cell cycle
            checkpoint, and cell volume control. Knockout studies in mice
            suggest that this kinase may be a fundamental regulator of cardiac
            contractility and Ca(2+) handling in myocytes. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.10531.1,
                                           SRR1660807.61550.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000413366.8/ ENSP00000408695.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-412               AC009452.21        135285-135696       c
            413-444             AC009452.21        132182-132213       c
            445-527             AC006263.1         79648-79730
            528-639             AC006947.2         76938-77049         c
            640-768             AC006947.2         72893-73021         c
            769-925             AC006947.2         31137-31293         c
            926-1060            AC006947.2         29968-30102         c
            1061-1157           AC006947.2         29357-29453         c
            1158-1295           AC005918.13        70654-70791         c
            1296-1469           AC005918.13        67817-67990         c
            1470-1561           AC005918.13        64624-64715         c
            1562-1624           AC005918.13        61758-61820         c
            1625-1763           AC005918.13        60719-60857         c
            1764-1844           AC005918.13        29412-29492         c
            1845-1952           AC005918.13        16505-16612         c
            1953-2093           AC005918.13        14500-14640         c
            2094-8964           AC005988.1         2398-9268
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q24.2"
     gene            1..8964
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /note="protein kinase C alpha"
                     /db_xref="GeneID:5578"
                     /db_xref="HGNC:HGNC:9393"
                     /db_xref="MIM:176960"
     exon            1..412
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            413..444
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            445..527
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            528..639
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            640..768
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            769..925
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1060
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1157
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1295
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1469
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1561
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1624
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1763
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1844
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1952
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2093
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..8964
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3517..3522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /note="hexamer: AATAAA"
     polyA_site      3539
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
     regulatory      8945..8950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /note="hexamer: AATAAA"
     polyA_site      8964
                     /gene="PRKCA"
                     /gene_synonym="AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-;
                     PRKACA"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtgagc ctggggccag cgggcgagcc agcggctccg gctcccgctc ccgctccgcg
       61 cagcaccagc ccgactctcc ccggcccccg ccgcgccccc tcgccgcgac ctcggccacc
      121 ggcccgcgcc ccgcgcccgg ggtcgccccg agcccgcacc tctcccccgc cgcccccgcc
      181 cacccggccc tccgcggccg cagctccccg gcggaggcaa gaggtggttg ggggggacca
      241 tggctgacgt tttcccgggc aacgactcca cggcgtctca ggacgtggcc aaccgcttcg
      301 cccgcaaagg ggcgctgagg cagaagaacg tgcacgaggt gaaggaccac aaattcatcg
      361 cgcgcttctt caagcagccc accttctgca gccactgcac cgacttcatc tgggggtttg
      421 ggaaacaagg cttccagtgc caagtttgct gttttgtggt ccacaagagg tgccatgaat
      481 ttgttacttt ttcttgtccg ggtgcggata agggacccga cactgatgac cccaggagca
      541 agcacaagtt caaaatccac acttacggaa gccccacctt ctgcgatcac tgtgggtcac
      601 tgctctatgg acttatccat caagggatga aatgtgacac ctgcgatatg aacgttcaca
      661 agcaatgcgt catcaatgtc cccagcctct gcggaatgga tcacactgag aagagggggc
      721 ggatttacct aaaggctgag gttgctgatg aaaagctcca tgtcacagta cgagatgcaa
      781 aaaatctaat ccctatggat ccaaacgggc tttcagatcc ttatgtgaag ctgaaactta
      841 ttcctgatcc caagaatgaa agcaagcaaa aaaccaaaac catccgctcc acactaaatc
      901 cgcagtggaa tgagtccttt acattcaaat tgaaaccttc agacaaagac cgacgactgt
      961 ctgtagaaat ctgggactgg gatcgaacaa caaggaatga cttcatggga tccctttcct
     1021 ttggagtttc ggagctgatg aagatgccgg ccagtggatg gtacaagttg cttaaccaag
     1081 aagaaggtga gtactacaac gtacccattc cggaagggga cgaggaagga aacatggaac
     1141 tcaggcagaa attcgagaaa gccaaacttg gccctgctgg caacaaagtc atcagtccct
     1201 ctgaagacag gaaacaacct tccaacaacc ttgaccgagt gaaactcacg gacttcaatt
     1261 tcctcatggt gttgggaaag gggagttttg gaaaggtgat gcttgccgac aggaagggca
     1321 cagaagaact gtatgcaatc aaaatcctga agaaggatgt ggtgattcag gatgatgacg
     1381 tggagtgcac catggtagaa aagcgagtct tggccctgct tgacaaaccc ccgttcttga
     1441 cgcagctgca ctcctgcttc cagacagtgg atcggctgta cttcgtcatg gaatatgtca
     1501 acggtgggga cctcatgtac cacattcagc aagtaggaaa atttaaggaa ccacaagcag
     1561 tattctatgc ggcagagatt tccatcggat tgttctttct tcataaaaga ggaatcattt
     1621 atagggatct gaagttagat aacgtcatgt tggattcaga aggacatatc aaaattgctg
     1681 actttgggat gtgcaaggaa cacatgatgg atggagtcac gaccaggacc ttctgtggga
     1741 ctccagatta tatcgcccca gagataatcg cttatcagcc gtatggaaaa tctgtggact
     1801 ggtgggccta tggcgtcctg ttgtatgaaa tgcttgccgg gcagcctcca tttgatggtg
     1861 aagatgaaga cgagctattt cagtctatca tggagcacaa cgtttcctat ccaaaatcct
     1921 tgtccaagga ggctgtttct gtctgcaaag gactgatgac caaacaccca gccaagcggc
     1981 tgggctgtgg gcctgagggg gagagggacg tgagagagca tgccttcttc cggaggatcg
     2041 actgggaaaa actggagaac agggagatcc agccaccatt caagcccaaa gtgtgtggca
     2101 aaggagcaga gaactttgac aagttcttca cacgaggaca gcccgtctta acaccacctg
     2161 atcagctggt tattgctaac atagaccagt ctgattttga agggttctcg tatgtcaacc
     2221 cccagtttgt gcaccccatc ttacagagtg cagtatgaaa ctcaccagcg agaacaaaca
     2281 cctccccagc ccccagccct ccccgcagtg ggaagtgaat ccttaaccct aaaattttaa
     2341 ggccacggcc ttgtgtctga ttccatatgg aggcctgaaa attgtagggt tattagtcca
     2401 aatgtgatca actgttcagg gtctctctct tacaaccaag aacattatct tagtggaaga
     2461 tggtacgtca tgctcagtgt ccagtttaat tctgtagaag ttacgtctgg ctctaggtta
     2521 acccttccta gaaagcaagc agactgttgc cccattttgg gtacaatttg atatactttc
     2581 cataccctcc atctgtggat ttttcagcat tggaatcccc caaccagaga tgttaaagtg
     2641 agcctgtccc aggaaacatc tccacccaag acgtctttgg aatccaagaa caggaagcca
     2701 agagagtgag cagggaggga ttgggggtgg gggaggcctc aaaataccga ctgcgtccat
     2761 tctctgcctc catggaaaca gcccctagaa tctgaaaggc cgggataaac ctaatcactg
     2821 ttcccaaaca ttgacaaatc ctaacccaac catggtccag cagttaccag tttaaacaaa
     2881 aaaacctcag atgagtgttg ggtgaatctg tcatctggta ccctccttgg ttgataactg
     2941 tcttgatact tttcattctt tgtaagaggc caaatcgtct aaggacgttg ctgaacaagc
     3001 gtgtgaaatc atttcagatc aaggataagc cagtgtgtac atatgttcat tttaatctct
     3061 gggagattat ttttccatcc agggtgccat cagtaatcat gccactactc accagtgttg
     3121 ttcaccaaca cccaccccca cacacaccaa cattttgctg cctaccttgt tatccttctc
     3181 aagaagctga agtgtacgcc ctctcccctt ttgtgcttat ttatttaata ggctgcagtg
     3241 tcgcttatga aagtacgatg tacagtaact taatggaagt gctgactcta gcatcagcct
     3301 ctaccgattg attttcctcc cttctctagc cctggatgtc cacttaggga taaaaagaat
     3361 atggttttgg ttcccatttc tagttcacgt tgaatgacag gcctggagct gtagaatcag
     3421 gaaacccgga tgcctaacag ctcaaagatg ttttgttaat agaaggattt taatacgttt
     3481 tgcaaatgca tcatgcaatg aattttgcat gtttataata aaccttaata acaagtgaat
     3541 ctatattatt gatataatcg tatcaagtat aaagagagta ttataataat tttataagac
     3601 acaattgtgc tctatttgtg caggttcttg tttctaatcc tcttttctaa ttaagtttta
     3661 gctgaatccc ttgcttctgt gctttccctc cctgcacatg ggcactgtat cagatagatt
     3721 actttttaaa tgtagataaa atttcaaaaa tgaatggcta gtttacgtga tagattaggc
     3781 tcttactaca tatgtgtgtg tatatatatg tatttgattc tacctgcaaa caaattttta
     3841 ttggtgagga ctatttttga gctgacactc cctcttagtt tcttcatgtc acctttcgtc
     3901 ctggttcctc cgccactctt cctcttgggg acaacaggaa gtgtctgatt ccagtctgcc
     3961 tagtacgttg gtacacacgt ggcattgccg cagcacctgg gctgaccttt gtgtgtgcgt
     4021 gtgtgtgtgt ttccttcttc ccttcagcct gtgactgttg ctgactccag gggtgggagg
     4081 gatggggaga ctcccctctt gctgtgtgta ctggacacgc aggaagcatg ctgtcttgct
     4141 gcctctgcaa cgacctgtcg tttgctccag catgcacaaa cttcgtgaga ccaacacagc
     4201 cgtgccctgc aggcaccagc acgtgctttt cagaggctgc ggactttctt ccagccattg
     4261 tggcattggc ctttccagtc ttgggaggag cgcgctgctt tggtgagaca cccccatgca
     4321 aggtcctcag agtagccggg ttctaccaca aacagaaaca gaatgaaagt agctgtcagt
     4381 ccttgtagag agccgctctg tttcctccca gaagcatctc ccagctaagc tcgcattatt
     4441 tttctcctct ggctgtttgc ctgaagttca cagaacacac aaccatgaaa ggctttttga
     4501 ggtgagaggc ccaggtggtc ctggcaaccc tgagtagaag gagagacggg gtagggaacg
     4561 ggcccggcca gaaaagaacc atttcttctg ccatctttta tgcaccatag acatcgagac
     4621 tccagggggt cctggctccc ctgtccctgc agccctgcag gtcagtgcat gatctgggtt
     4681 cgtgtcctga ccaggtgctc ctcctttgat ccgaggggaa agggactggt ttatagaaag
     4741 agcctaggag acaaaagggc cagtccccct gcccagaatg gagcagcagc aggacagacc
     4801 cccacgaggc cccccagaga ggaggaagat cccacggagg aacacatgag gttagggacc
     4861 cttgttcagc accccaaaca gcctgcctgt ttaaagcagg cagcaggctt aggccttccc
     4921 tgcaacccca acacccacaa gtttgtttct ctaggaaaca cattcactgt ctcagctggc
     4981 tgttactctc tcagaccata tggcaaagtt ttccaagaaa atgccccgac aggggtgccc
     5041 agcacactgc ctgagggaca acagacatca gaacaaaccc ccagagagaa acagtcaaaa
     5101 tcagggcccg gtgcagtgtt gtcatgtgga acctgcttta tccattgctg agtgttgaat
     5161 gtgggtaatg gttagggctt tccagatctc agcagccaaa gacagttatt gttggaagac
     5221 tgtcatgtag ataaccatga gcaatggctc gcctcagaat cagttcataa aattctatgg
     5281 tactggcccc ttcgtgggta ttgtgtgaaa tgagatggtg gcgaggggtg cgctgtggaa
     5341 ctgccgcagc cacgcaggag gtccctgggg gatgctttgg gaagtccttg cccctgagca
     5401 ctgcctgatt gccagggcct gtggaggtct aggccgcctg gcagaatcta gcaccgtccg
     5461 aatccccgca ggacccatgg agctatgacc acaccaggcc attcaaatgg ctctgcatta
     5521 tcttcccttg gaaggtggcc actcctcggt ggcagggcct ttccctgagg ctgcaggccg
     5581 tgggctggca gcccgtctct tggcatttca attgaaggtc accaggtgct gggtttgaaa
     5641 ggaagtcact ggagtgctgc caggggccgc cctccaaggt taatgagagg cccacatcca
     5701 ggcaagaact aattcaaaag gcagatcaga aaccacagga gtcaaaatta ttgctccggc
     5761 agtgcttccc ttcctttcat ccactggcct cgtgtggtcc atgcagggcc actgtctgcc
     5821 ctttctgatg ccacgtatta ggctttctta ctcagaattt tgatagaaaa ccatggggcc
     5881 aagagctctg gaagcctggc cggaaagacc aaggttcatg cagcccaaca aatgattgtt
     5941 gagcacctct cggagccaaa gtccttaggc gagtgtggtg acttcctgga aggaggatgc
     6001 agacttccag agagcccccc caacggacgt gctgagaagg gagagggagg cgggggctgt
     6061 agtcaggaag gagccagaga agaacagggt ttgggtgcat ccagaaatat gcctgcagta
     6121 ggagggagag gaaggggtgc caccgtcaac ggcttcccat cggaggtggt tggtgcagat
     6181 ggaagtttct gtctgctggc cctcaagaga gtgttttgcc agggacacag tctgttcctc
     6241 ctcagaaaac accccccaaa tgctaacaac atccccacca gctgctagaa gcccctttcc
     6301 cctccccacc ttgaagtagc tcatagttct ctgggcagag ccagaccatc cagtgtaccc
     6361 cagaggccag taggttcctg cccattttcc tctctggctt cctgccaaga attatggcag
     6421 ctgaggatga atggagaagt aaaaacaact aacaccgcac aactaacaac taacaccgca
     6481 gttcccacct gggttccact tagcaggaga catttcggag ggtttttttt gtttttgttc
     6541 ctgttttttt tttttttgct ggaatttgtt ttctcagtac tgaaaagaga aaaagtgaca
     6601 atcttgtatt tttaaaagcc tcggaaaggt gataccatct gacagtcatt ttctcacgtt
     6661 ggtcttctaa agtcacctat ttcttgtgtg tgcacatcac accatttcct gtttctttat
     6721 aacccgacaa gggtaggagt gcctgtttcc cctgctgggc acaccagaca atcgtaatca
     6781 caaaacagac actgagccag gggcccaaag ggtgtgatca tgagagttac cgggacagca
     6841 gtaggcatga cagtcaccag gaaggacaag ggtgctctgt tgttagtggc cacacaccaa
     6901 tttgacaagg agtgttgcga aatttttatt tatttattta tttattttga gatggagttt
     6961 cactcttgtt gcccaggctg gagtgcggtg gtacaatctc ggctcactgc aacctccacc
     7021 tcccaggttc aagcgattct cctgcctcag cctcccaagt acctgggact acaggtgcgt
     7081 gccaccacac ccagctaaat tttgtgtttt tagtagagat ggggtttcac catgttggcc
     7141 aggatggtct tgaacccctg acctcatgat ctgcctgcct cggcctccca aagtgctggg
     7201 attacaggca tgagccacca cgcccagcca aaatattttt ttaaagtcat tttccttaag
     7261 ctgcttgggc tacatgtgaa atacactgga cggtcaacat tcctgtctcc tcccatttgg
     7321 gctgatgcag cagatccagg gaatgttacc tgtttctgct gctagaagat ccaggaaatt
     7381 gggaaggtta cctgacgcac acatggatga aggccatcat ctagaaatgg ggtcaaccac
     7441 aattgtgtta attccgtagt gtcagggatt cttcgggaag gtcaacagta tgaaggattc
     7501 tgacccctgt gcctcccatt tatgtgatca ggtgacagtt aataaccgtg gaggtcacac
     7561 tcagccatcc aacagcctta cagtgaccct acacaaaagc ccccaaattc caaagacttt
     7621 ttcttaacct aaaggaagaa attatttgtt aattccagta gagcaactga atatactggg
     7681 ctatttgtac ttttttatag agaactttaa taataattct ttaaaaatga gtttttagaa
     7741 caaagcaact gacgatttcc taagattcca atgccctgga gcttgtagga ggacttagcc
     7801 tgggtcagct ggagcacccc cgacctgatc tcccactgcc agattttccc atgctcctag
     7861 ggtatggagt ccacgtggga atgactgcaa gttcaggtgg aacttggccg actgatgctc
     7921 tgcgagtttt taatagacac tggggacaac tgcttaaggt ttagaaactt ccaaaccaca
     7981 ggaaagacat ttttagtgtc ccccatccag aggcagccct ggaataggat tcccaggggt
     8041 ttctgggacc cctttccttg ctccgtgagg ctctgtggcc atcttttggc aggaggagga
     8101 tgcttccttg gctctgtgcc cagacccgcc tggtccccag gtctctcacc ttgggtgaag
     8161 attcagagat gccctgtaag gattttgccc actgggcaac tcagaaatac ttcgatctcc
     8221 caagatataa gaggcagcag caaacgtgcc tattgacgtc tgtttcatag ttaccactta
     8281 cgcgagtaga cagaactcgg cttttcagaa aataggtgtc aagtccactt tataagaacc
     8341 tttttttcta aaataagata aaaggtggct ttgcattttc tgattaaacg actgtgtctt
     8401 tgtcacctct gcttaacttt aggagtatcc attcctgtga ttgtagactt ttgttgatat
     8461 tcttcctgga agaatatcat tcttttcttg aagggttggt ttactagaat attcaaaatc
     8521 aatcatgaag gcagttacta ttttgagtct aaaggttttc taaaaattaa cctcacatcc
     8581 cttctgttag ggtctttcag aatatctttt ataaacagaa gcatttgaag tcattgcttt
     8641 tgctacatga tttgtgtgtg tgaaggacat accacgttta aatcattaat tgaaaaacat
     8701 catataagcc ccaactttgt ttggaggaag agacggaggt tgaggttttt ccttctgtat
     8761 aagcacctac tgacaaaatg tagaggccat tcaaccgtca aacaccattt ggttatatcg
     8821 cagaggagac ggatgtgtaa attactgcat tgcttttttt ttcagtttgt ataacctcta
     8881 atctccgttt gcatgatacg ctttgttaga aacattaatt gtagtttgga agcaagtgtg
     8941 tatgaataaa gataatgatc attc
//
