LOCUS       NM_004157               6492 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein kinase cAMP-dependent type II regulatory
            subunit alpha (PRKAR2A), transcript variant 1, mRNA.
ACCESSION   NM_004157
VERSION     NM_004157.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ruland,L., Andreatta,F., Massalini,S., Chuva de Sousa Lopes,S.,
            Clevers,H., Hendriks,D. and Artegiani,B.
  TITLE     Organoid models of fibrolamellar carcinoma mutations reveal
            hepatocyte transdifferentiation through cooperative BAP1 and
            PRKAR2A loss
  JOURNAL   Nat Commun 14 (1), 2377 (2023)
   PUBMED   37137901
  REMARK    GeneRIF: Organoid models of fibrolamellar carcinoma mutations
            reveal hepatocyte transdifferentiation through cooperative BAP1 and
            PRKAR2A loss.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 6492)
  AUTHORS   Zapata-Carmona,H., Baron,L., Kong,M. and Morales,P.
  TITLE     Protein Kinase A (PRKA) Activity Is Regulated by the Proteasome at
            the Onset of Human Sperm Capacitation
  JOURNAL   Cells 10 (12), 3501 (2021)
   PUBMED   34944009
  REMARK    GeneRIF: Protein Kinase A (PRKA) Activity Is Regulated by the
            Proteasome at the Onset of Human Sperm Capacitation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6492)
  AUTHORS   Zorzan,M., Del Vecchio,C., Vogiatzis,S., Saccon,E., Parolin,C.,
            Palu,G., Calistri,A. and Mucignat-Caretta,C.
  TITLE     Targeting the Regulatory Subunit R2Alpha of Protein Kinase A in
            Human Glioblastoma through shRNA-Expressing Lentiviral Vectors
  JOURNAL   Viruses 13 (7), 1361 (2021)
   PUBMED   34372567
  REMARK    GeneRIF: Targeting the Regulatory Subunit R2Alpha of Protein Kinase
            A in Human Glioblastoma through shRNA-Expressing Lentiviral
            Vectors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6492)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 6492)
  AUTHORS   Carr,D.W., Hausken,Z.E., Fraser,I.D., Stofko-Hahn,R.E. and
            Scott,J.D.
  TITLE     Association of the type II cAMP-dependent protein kinase with a
            human thyroid RII-anchoring protein. Cloning and characterization
            of the RII-binding domain
  JOURNAL   J Biol Chem 267 (19), 13376-13382 (1992)
   PUBMED   1618839
REFERENCE   7  (bases 1 to 6492)
  AUTHORS   Skalhegg,B.S., Landmark,B., Foss,K.B., Lohmann,S.M., Hansson,V.,
            Lea,T. and Jahnsen,T.
  TITLE     Identification, purification, and characterization of subunits of
            cAMP-dependent protein kinase in human testis. Reverse mobilities
            of human RII alpha and RII beta on sodium dodecyl
            sulfate-polyacrylamide gel electrophoresis compared with rat and
            bovine RIIs
  JOURNAL   J Biol Chem 267 (8), 5374-5379 (1992)
   PUBMED   1544918
REFERENCE   8  (bases 1 to 6492)
  AUTHORS   Scott,J.D., Stofko,R.E., McDonald,J.R., Comer,J.D., Vitalis,E.A.
            and Mangili,J.A.
  TITLE     Type II regulatory subunit dimerization determines the subcellular
            localization of the cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 265 (35), 21561-21566 (1990)
   PUBMED   2147685
REFERENCE   9  (bases 1 to 6492)
  AUTHORS   Hofmann,B., Nishanian,P., Baldwin,R.L., Insixiengmay,P., Nel,A. and
            Fahey,J.L.
  TITLE     HIV inhibits the early steps of lymphocyte activation, including
            initiation of inositol phospholipid metabolism
  JOURNAL   J Immunol 145 (11), 3699-3705 (1990)
   PUBMED   1978848
REFERENCE   10 (bases 1 to 6492)
  AUTHORS   Taylor,S.S., Buechler,J.A. and Yonemoto,W.
  TITLE     cAMP-dependent protein kinase: framework for a diverse family of
            regulatory enzymes
  JOURNAL   Annu Rev Biochem 59, 971-1005 (1990)
   PUBMED   2165385
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC144546.9, DB040049.1,
            CF994284.1, X14968.1, BC002763.2, AC141002.8 and AA774111.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004157.3.
            
            Summary: cAMP is a signaling molecule important for a variety of
            cellular functions. cAMP exerts its effects by activating the
            cAMP-dependent protein kinase, which transduces the signal through
            phosphorylation of different target proteins. The inactive kinase
            holoenzyme is a tetramer composed of two regulatory and two
            catalytic subunits. cAMP causes the dissociation of the inactive
            holoenzyme into a dimer of regulatory subunits bound to four cAMP
            and two free monomeric catalytic subunits. Four different
            regulatory subunits and three catalytic subunits have been
            identified in humans. The protein encoded by this gene is one of
            the regulatory subunits. This subunit can be phosphorylated by the
            activated catalytic subunit. It may interact with various A-kinase
            anchoring proteins and determine the subcellular localization of
            cAMP-dependent protein kinase. This subunit has been shown to
            regulate protein transport from endosomes to the Golgi apparatus
            and further to the endoplasmic reticulum (ER). [provided by RefSeq,
            Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.211463.1,
                                           SRR1660805.81083.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265563.13/ ENSP00000265563.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC144546.9         45598-45621         c
            25-37               DB040049.1         1-13
            38-545              CF994284.1         28-535
            546-1832            X14968.1           457-1743
            1833-2418           BC002763.2         1721-2306
            2419-6344           AC141002.8         81458-85383         c
            6345-6492           AA774111.1         24-171              c
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..6492
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /note="protein kinase cAMP-dependent type II regulatory
                     subunit alpha"
                     /db_xref="GeneID:5576"
                     /db_xref="HGNC:HGNC:9391"
                     /db_xref="MIM:176910"
     exon            1..540
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..1493
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1;
                     protein kinase A, RII-alpha subunit; cAMP-dependent
                     protein kinase regulatory subunit RII alpha;
                     cAMP-dependent protein kinase type II-alpha regulatory
                     subunit; protein kinase, cAMP-dependent, regulatory
                     subunit type II alpha"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase type II-alpha
                     regulatory subunit isoform a"
                     /protein_id="NP_004148.1"
                     /db_xref="CCDS:CCDS2778.1"
                     /db_xref="GeneID:5576"
                     /db_xref="HGNC:HGNC:9391"
                     /db_xref="MIM:176910"
                     /translation="MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREAR
                     APASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCA
                     ETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIV
                     KADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRA
                     ATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDV
                     IGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQ
                     YFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKM
                     FGSSVDLGNLGQ"
     exon            541..576
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..629
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            630..713
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            714..820
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            821..974
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1076
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1151
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1217
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1359
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..6492
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6474..6479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /note="hexamer: AATAAA"
     polyA_site      6492
                     /gene="PRKAR2A"
                     /gene_synonym="PKR2; PRKAR2"
                     /note="major polyA site"
ORIGIN      
        1 acaaccagga cgcctgcccc gcccgtccag ctcgcctgcc cgtcggtccc cgcggcggcg
       61 gcggctgccc agtgacagcg gcggcggtgc caggtcggcc gtggtagcgt agggttgcgc
      121 ggcccggaaa cgcagagccg gccaaagagc ggcgcgacgt gagccggggc cgtgcgcgaa
      181 gagacctcgc gggcgcggag cgaaaggccg gcgtgagtga gcgcggagac agtggccgcc
      241 ggcggcccaa cccgtctatc ccttcggccg ccgccggcat gagccacatc cagatcccgc
      301 cggggctcac ggagctgctg cagggctaca cggtggaggt gctgcgacag cagccgcctg
      361 acctcgtcga attcgcagtg gagtacttca cccgcctgcg cgaggcccgc gccccagcct
      421 cagtcctgcc cgccgccacc ccacgccaga gcctgggcca ccccccgcca gaacccggcc
      481 cggaccgtgt cgccgacgcc aaaggggaca gcgagtcgga ggaggacgag gacttggaag
      541 ttccagttcc tagcagattt aatagacgag tatcagtctg tgctgagacc tataaccctg
      601 atgaggaaga ggaagataca gatccaaggg tgattcatcc taaaactgat gaacagagat
      661 gcagacttca ggaagcttgc aaagatattc tccttttcaa aaatcttgat caggaacagc
      721 tttctcaagt tctcgatgcc atgtttgaaa ggatagtcaa agctgatgag catgtcattg
      781 accaaggaga tgatggagac aacttttatg tcatagaacg gggaacttat gacattttag
      841 taacaaaaga taatcaaacc cgctctgttg gtcaatatga caaccgtggc agttttggag
      901 aactagctct gatgtacaac accccgagag ctgctaccat tgttgctacc tcagaaggct
      961 ccctttgggg actggaccgg gtgactttta gaagaatcat agtgaaaaat aatgcaaaga
     1021 agaggaagat gtttgaatca tttattgagt ctgtgcccct ccttaaatca ctagaggtgt
     1081 cagaacgaat gaagattgtg gatgtaatag gagagaagat ctataaggat ggagaacgca
     1141 taatcactca gggtgaaaag gctgatagct tttacatcat agagtctggc gaagtgagca
     1201 tcttgattag aagcaggact aaatcaaaca aggatggtgg gaaccaggag gtcgagattg
     1261 cccgctgcca taaggggcag tactttggag agcttgccct ggtcaccaac aaacccagag
     1321 ctgcctcagc ttatgcagtt ggagatgtca aatgcttagt tatggatgta caagcattcg
     1381 agaggcttct ggggccctgc atggacatca tgaagaggaa catctcacac tatgaggaac
     1441 agctggtgaa gatgtttggc tccagcgtgg atctgggcaa cctcgggcag taggtgtgcc
     1501 acaccccaga gccttcttag tgtgacacca aaaccttctg gtcagccaca gaacacatac
     1561 agaaaacaga catgacagaa ctgttcctgc cgttgccgcc actgctgcca ttgctgtggt
     1621 tatgggcatt tagaaaactt gaaagtcagc actaaaggat gggcagaggt tcaacccaca
     1681 cctccacttt gcttctgaag gcccattcat tagaccactt gtaaagatta ctccaaccca
     1741 gtttttatat ctttggttca aaacggcatg tctctccaac aatttaagtg cctgatacaa
     1801 agtccaaagt ataaacatgc tcctttcctc tcttgctgct actcttgctt ttggaagtta
     1861 ccacagggtc tgcagaaacc tgttgtataa ctgtagacac tctctaatgg ttctcaaagg
     1921 aggaaatgta gccttcagtc tcctcatttg tcctttgagg aagtccacat ttgttcacag
     1981 ttgcagcctt tggttttaca gtgggaaatg gtggtggatg atatggacat atgtagccca
     2041 gtggcattgt actttctgct gacagctgca cacattacag ctgtctccaa acccacagtg
     2101 atgcttaggg aaagaccctg ctcaggaccc agcaggtcag caccccagag cagactgata
     2161 ggtccgtggg acccatgtta gagcagaaaa tttgggctca gcacatttta ctgttagtag
     2221 agagccagga aacgttttct gggttgggga ttttgtggga ttttttaatt tttttagtag
     2281 gttttgttta acctctgtgc agtttgtatg aatgaattgc tatacattta taaggagcca
     2341 gggtctggag ggttgctatc actttgtcca gcccaaatac cttcctgggc aactcctacc
     2401 atttgtttgc agttgcctct actagctgat ggcagtatgc tggaaagagg ttgtactata
     2461 aagagagttc tttccttcta ctccagagtt gttgtgtagc tttgccattg aaccgatcaa
     2521 tttttaaact ctttaaagaa gcagcagcta tttttttgaa attaaagaaa attctcaggc
     2581 caggctcaat ggctcatgcc tataattgta gtgctttggg aggctgagct gggaggatcg
     2641 cttaagacca gcctaggcaa cagagtgaga tcctgtctct acaaaaacaa aaattttttt
     2701 tttgttttct ttttgagacg gagtttcgct cttgttgccc aggctggagt gcaatggtgc
     2761 ggtcttggct caccgcaacc tccgcctccc agattaaagt gattctcctg cctcaacctc
     2821 ctgagtagct gggattacag gcatgcgcca ccatgccggg cgaattttgt atttttaata
     2881 gagacagggt ttctctgtgt tggtcaggct ggtctcaaac tcccaacctc aggtgatccg
     2941 cccacctcag cctctcagag tgctgggatt acagatgtga gccactgcac ctggcaaaaa
     3001 aaaaattttt ttttaatttg tcaggtgtgg tgatgcatgc atgtagtccc aaatacttag
     3061 gaggctgagg tggaaggatc acttgagccc aggaggtcaa ggcaacagtg agctgtgatc
     3121 atgccactgc actccagtct gggtgacaca gtgagaccct gtctcttaaa aaaaaaaaaa
     3181 gaaagaaaaa aaattattct ttaggtaatt tcacttggac atattcatgt atatggagtt
     3241 tgtcagctga ctcaggcatt taccctaaag atcaaatctg ttttcaaaaa ttggcattgg
     3301 ccaggcacgg tgacccacac ctgtaatccc agcactttgg gaggccgagg cgggcggatc
     3361 acgaggtcag gggttcaaga ccagcctggc caacatagtg aaaccccgtc tctactaaaa
     3421 atacaaaaaa ttagctgggc atggtggtgg gcgcctgtaa tcccagctac ttgagaggct
     3481 caggcaggag aatcgcttga acctgggagt ggaggttgca gtgagccgag attgcaccat
     3541 tgtattccac cccgggtgac agtgcaagac tccatctcaa aaaaaaaaaa aaaatttggc
     3601 atcatttaca atttcataga attactgtga aggcctttct agttgagatg ttggggtatt
     3661 tgggattcta attgttaacc ccagaagaag gtaatttagc ttgtatttat ttaaaaccca
     3721 tttagccttt tacttatatc tggtagaatt ccagtgatca tcctaataag gtatatttca
     3781 gaataatttt tttttccttc agaataactt agaatcagat gctataaggg ctcctaggag
     3841 cagtgtgaaa tttccgtaaa gataaatttg aatgttgtaa ccaagtttat attaaaccaa
     3901 gaggccattt ccaatatgat tttttgtttc tttttaactt gttaagtccc taagagatta
     3961 catgctaggg cttgagtcat ttctattgta gataatgatg gcccacacag tcaccttcaa
     4021 ctatccacat aagctaggct ttccgctttt gccacggaca gtgtgaccaa gatatttcca
     4081 gagtaaataa cccaccacaa ccttggtaat tcctcttttc ttcttaagct ccaggaagcg
     4141 aaagcagaag gactcttttc agactgccct ctgtagccta cattgcagct ttccaaaaca
     4201 ggcagctagc actgggaaag cccatgtggt gaccccatat ttttctgagg ttcttctttt
     4261 ccatggtgtt actttattat cagaaagtaa attcagaaaa caggtcttgc ccttagcaga
     4321 caagaaccac accagtttct tgtaaaggta acggatacat tgggattcag gagtgacaca
     4381 gaggtccagc cccagaactt gtaaggattt tgtttgaaca ctgagcagat gcctcctccc
     4441 tgccacccat cacactagtt agggctggcc atgaattcta tgccagagtc actcctgcag
     4501 tctgctaggg atgggccttc ttatcccact ctcgcacaca tcccagtcta gtctttgcct
     4561 tcacagagtc ctccttgaca cccctgactt aatgatagtt gctgttttgg agtagaattg
     4621 atcaggttta agtcatcctg ctcaggttgg gcatagtggc tcatgcctgt aatctcagca
     4681 ctttgggaag ccaaagtggg aggattgctt gagcccagga gttccaaacc atcctgggca
     4741 acagagggag accctgtctc taccaagaaa aaaaaaaaaa aaaaaagtta aaaaaacaat
     4801 tagctggacc tggtggtgca cactcagtag gctgaggtga aaggattcct ttaacctggg
     4861 agactgaagc tgcagtgagc catgatcgtg ccactgcact ccagtctggg ggacaaagtg
     4921 ggaccctatc tcaaaaaaaa aaaaaaaaag tcatcctcct ccaactactg attgacatat
     4981 ggatcatcag ttcagaccct cactgcatac aggatcccta gagggttcta tttaatagct
     5041 tgaggaaggg agactttaaa aggacgtgtg tgagtgaaat aggatatagc cattaccacg
     5101 gtgccaggac ctgacagcgt tccaattctt tttgcagcat ggggaatcaa aggtggcatg
     5161 ccaagttcaa ctcagggctg aggtatccac attgtccaca tcaggcaagc cctgcactga
     5221 cggttgagcc tcatggagag gagcatgtgt tggaaagaga tccctttgtt aactgttttg
     5281 tggtgttctc ttcaatgaat tagagctcat gccccttttc tggctttgct gttgattttg
     5341 gatggtagag aatattcctg agagccttcc ttttggcccc cagcttatgc cacccactct
     5401 cttctcttgg ttgaattctc tgaaggaaag gttcatgtgc tattgtcctg ttagtcaata
     5461 gtcttcatat ataattgtgt tacatatatt gctgtagact ctcagaaatc agggtagagc
     5521 ttttcctttg agcagtttaa tgagtgaatt cagcagcaaa gtcgcaagaa atggttctcc
     5581 agccaggaga ggttatgttt atcctctgat tgcccgtttt ctctgcacac agtgatatcg
     5641 tattcagtga gaggtgctgt tggcacccag cagcaccctg ggcacacagc atttcatgtc
     5701 atgtcacagt gtacaagcta ccctctaatt cagaaagaag agcattttgc acagagaaaa
     5761 ataaaaagat ccatgaatgt catcttttat cttttatttt cagttggctg atgttggaat
     5821 ttttgttctt gtcatgaact tgtaaaccaa tcttgccaag atacaagttg ttttggtttt
     5881 tcactacaat gacctcttgt tcctcctgtc ttgactgctg acgttcctca atgattctat
     5941 tgtctatttt atgggaagca gccttcccat aggtttcctt ttacacactg cagggctatc
     6001 tttatacttt aaaaaaaaaa aaaaaaaaaa aaaaaaggac aagaactgtc actaacctca
     6061 tggaggggtt tgcgtaaaac catttagccc accttgagca aagggtagat tccgtgttgt
     6121 ttttttaagc tcactgtaat aaaataaatc taattcagca ttattgtgct acctcaaagg
     6181 taaaaaatgt tttaaggtct tcttttggtc ctgagttcta tatacagtgt ttgaaatgtc
     6241 cttcatttgg aattattttt taaattcttg gagtgaattt tattttaatc tgttttaatc
     6301 ttgtatttta atctcagaag aataagtgat tgaaacgtga tcaattcttg ctctgtggtg
     6361 ttaaacatat aatgaacagt cattaagaat taagtcactg tttgccataa acaaggttga
     6421 tgttcttttt gttgttgtta aggaaaccct agggctcggc tttactcttg attaataaag
     6481 gctgacaaat ca
//
