LOCUS       NM_017431               2388 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens protein kinase AMP-activated non-catalytic subunit
            gamma 3 (PRKAG3), mRNA.
ACCESSION   NM_017431
VERSION     NM_017431.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Eskesen,N.O., Kjobsted,R., Birk,J.B., Henriksen,N.S.,
            Andersen,N.R., Ringholm,S., Pilegaard,H. and Wojtaszewski,J.F.P.
  TITLE     The human AMPKgamma3 R225W mutation negatively impacts site-1
            nucleotide binding and does not enhance basal AMPKgamma3-associated
            activity nor glycogen production in human or mouse skeletal muscle
  JOURNAL   Acta Physiol (Oxf) 240 (10), e14213 (2024)
   PUBMED   39171449
  REMARK    GeneRIF: The human AMPKgamma3 R225W mutation negatively impacts
            site-1 nucleotide binding and does not enhance basal
            AMPKgamma3-associated activity nor glycogen production in human or
            mouse skeletal muscle.
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Kido,K., Eskesen,N.O., Henriksen,N.S., Onslev,J., Kristensen,J.M.,
            Larsen,M.R., Hingst,J.R., Knudsen,J.R., Birk,J.B., Andersen,N.R.,
            Jensen,T.E., Pehmoller,C., Wojtaszewski,J.F.P. and Kjobsted,R.
  TITLE     AMPKgamma3 Controls Muscle Glucose Uptake in Recovery From Exercise
            to Recapture Energy Stores
  JOURNAL   Diabetes 72 (10), 1397-1408 (2023)
   PUBMED   37506328
  REMARK    GeneRIF: AMPKgamma3 Controls Muscle Glucose Uptake in Recovery From
            Exercise to Recapture Energy Stores.
REFERENCE   3  (bases 1 to 2388)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2388)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 2388)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2388)
  AUTHORS   Jeon,J.T., Park,E.W., Jeon,H.J., Kim,T.H., Lee,K.T. and Cheong,I.C.
  TITLE     A large-insert porcine library with sevenfold genome coverage: a
            tool for positional cloning of candidate genes for major
            quantitative traits
  JOURNAL   Mol Cells 16 (1), 113-116 (2003)
   PUBMED   14503854
REFERENCE   7  (bases 1 to 2388)
  AUTHORS   Nielsen,J.N., Mustard,K.J., Graham,D.A., Yu,H., MacDonald,C.S.,
            Pilegaard,H., Goodyear,L.J., Hardie,D.G., Richter,E.A. and
            Wojtaszewski,J.F.
  TITLE     5'-AMP-activated protein kinase activity and subunit expression in
            exercise-trained human skeletal muscle
  JOURNAL   J Appl Physiol (1985) 94 (2), 631-641 (2003)
   PUBMED   12391032
REFERENCE   8  (bases 1 to 2388)
  AUTHORS   Milan,D., Jeon,J.T., Looft,C., Amarger,V., Robic,A., Thelander,M.,
            Rogel-Gaillard,C., Paul,S., Iannuccelli,N., Rask,L., Ronne,H.,
            Lundstrom,K., Reinsch,N., Gellin,J., Kalm,E., Roy,P.L., Chardon,P.
            and Andersson,L.
  TITLE     A mutation in PRKAG3 associated with excess glycogen content in pig
            skeletal muscle
  JOURNAL   Science 288 (5469), 1248-1251 (2000)
   PUBMED   10818001
  REMARK    GeneRIF: Functional analysis of the pig counterpart.
REFERENCE   9  (bases 1 to 2388)
  AUTHORS   Cheung,P.C., Salt,I.P., Davies,S.P., Hardie,D.G. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase gamma-subunit
            isoforms and their role in AMP binding
  JOURNAL   Biochem J 346 Pt 3 (Pt 3), 659-669 (2000)
   PUBMED   10698692
REFERENCE   10 (bases 1 to 2388)
  AUTHORS   Woods,A., Cheung,P.C., Smith,F.C., Davison,M.D., Scott,J.,
            Beri,R.K. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase beta and gamma
            subunits. Assembly of the heterotrimeric complex in vitro
  JOURNAL   J Biol Chem 271 (17), 10282-10290 (1996)
   PUBMED   8626596
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009974.10.
            
            On Jul 16, 2021 this sequence version replaced NM_017431.3.
            
            Summary: The protein encoded by this gene is a regulatory subunit
            of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
            consisting of an alpha catalytic subunit, and non-catalytic beta
            and gamma subunits. AMPK is an important energy-sensing enzyme that
            monitors cellular energy status. In response to cellular metabolic
            stresses, AMPK is activated, and thus phosphorylates and
            inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
            beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
            regulating de novo biosynthesis of fatty acid and cholesterol. This
            subunit is one of the gamma regulatory subunits of AMPK. It is
            dominantly expressed in skeletal muscle. Studies of the pig
            counterpart suggest that this subunit may play a key role in the
            regulation of energy metabolism in skeletal muscle. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ249977.1, SRR14038193.1239984.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158800, SAMEA2162946
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000439262.7/ ENSP00000397133.3
            NMD candidate          :: translation inferred from conservation
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                AC009974.10        169822-169887       c
            67-106              AC009974.10        169420-169459       c
            107-262             AC009974.10        168830-168985       c
            263-666             AC009974.10        168062-168465       c
            667-748             AC009974.10        166603-166684       c
            749-807             AC009974.10        166088-166146       c
            808-853             AC009974.10        165917-165962       c
            854-908             AC009974.10        165659-165713       c
            909-1035            AC009974.10        165331-165457       c
            1036-1201           AC009974.10        165012-165177       c
            1202-1239           AC009974.10        162623-162660       c
            1240-1386           AC009974.10        162306-162452       c
            1387-1619           AC009974.10        161730-161962       c
            1620-2388           AC009974.10        160392-161160       c
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..2388
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /note="protein kinase AMP-activated non-catalytic subunit
                     gamma 3"
                     /db_xref="GeneID:53632"
                     /db_xref="HGNC:HGNC:9387"
                     /db_xref="MIM:604976"
     exon            1..66
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1503
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /note="5'-AMP-activated protein kinase, gamma-3 subunit;
                     AMPK gamma-3 chain; AMPK gamma3; AMPK subunit gamma-3;
                     protein kinase, AMP-activated, gamma 3 non-catalytic
                     subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit gamma-3"
                     /protein_id="NP_059127.2"
                     /db_xref="CCDS:CCDS2424.1"
                     /db_xref="GeneID:53632"
                     /db_xref="HGNC:HGNC:9387"
                     /db_xref="MIM:604976"
                     /translation="MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTS
                     SSERIRGKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLAQA
                     DPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELEGLLEERPALC
                     LSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTMLEIKKAFFA
                     LVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIY
                     LQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIF
                     GSLLPRPSFLYRTIQDLGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQV
                     VGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIARE
                     QVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA"
     exon            67..106
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            107..262
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            263..666
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            667..748
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            749..807
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            808..853
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            854..908
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1035
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1201
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1239
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1386
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1619
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..2388
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2369..2374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /note="hexamer: AAGAAA"
     polyA_site      2388
                     /gene="PRKAG3"
                     /gene_synonym="AMPKG3; SMGMQTL"
                     /note="major polyA site"
ORIGIN      
        1 acacttgctc tcagttggtc tggggctggc cacatggagc ccgggctgga gcacgcactg
       61 cgcaggaccc cttcctggag cagccttggg ggttctgagc atcaagagat gagcttccta
      121 gagcaagaaa acagcagctc atggccatca ccagctgtga ccagcagctc agaaagaatc
      181 cgtgggaaac ggagggccaa agccttgaga tggacaaggc agaagtcggt ggaggaaggg
      241 gagccaccag gtcaggggga aggtccccgg tccaggccag ctgctgagtc caccgggctg
      301 gaggccacat tccccaagac cacacccttg gctcaagctg atcctgccgg ggtgggcact
      361 ccaccaacag ggtgggactg cctcccctct gactgtacag cctcagctgc aggctccagc
      421 acagatgatg tggagctggc cacggagttc ccagccacag aggcctggga gtgtgagcta
      481 gaaggcctgc tggaagagag gcctgccctg tgcctgtccc cgcaggcccc atttcccaag
      541 ctgggctggg atgacgaact gcggaaaccc ggcgcccaga tctacatgcg cttcatgcag
      601 gagcacacct gctacgatgc catggcaact agctccaagc tagtcatctt cgacaccatg
      661 ctggagatca agaaggcctt ctttgctctg gtggccaacg gtgtgcgggc agcccctcta
      721 tgggacagca agaagcagag ctttgtgggg atgctgacca tcactgactt catcctggtg
      781 ctgcatcgct actacaggtc ccccctggtc cagatctatg agattgaaca acataagatt
      841 gagacctgga gggagatcta cctgcaaggc tgcttcaagc ctctggtctc catctctcct
      901 aatgatagcc tgtttgaagc tgtctacacc ctcatcaaga accggatcca tcgcctgcct
      961 gttcttgacc cggtgtcagg caacgtactc cacatcctca cacacaaacg cctgctcaag
     1021 ttcctgcaca tctttggttc cctgctgccc cggccctcct tcctctaccg cactatccaa
     1081 gatttgggca tcggcacatt ccgagacttg gctgtggtgc tggagacagc acccatcctg
     1141 actgcactgg acatctttgt ggaccggcgt gtgtctgcac tgcctgtggt caacgaatgt
     1201 ggtcaggtcg tgggcctcta ttcccgcttt gatgtgattc acctggctgc ccagcaaacc
     1261 tacaaccacc tggacatgag tgtgggagaa gccctgaggc agaggacact atgtctggag
     1321 ggagtccttt cctgccagcc ccacgagagc ttgggggaag tgatcgacag gattgctcgg
     1381 gagcaggtac acaggctggt gctagtggac gagacccagc atctcttggg cgtggtctcc
     1441 ctctccgaca tccttcaggc actggtgctc agccctgctg gcatcgatgc cctcggggcc
     1501 tgagaagatc tgagtcctca atcccaagcc acctgcacac ctggaagcca atgaagggaa
     1561 ctggagaact cagccttcat cttcccccac ccccatttgc tggttcagct atgattcagg
     1621 cttcttcagc cctcccaaat tgcccttgcc ctacctgtgc tcccagaagc cctcgggcat
     1681 gcccagtgca ccatgggatg atgaaattaa ggagaacagc tgagtcaagc ttggaggtcc
     1741 ctgaaccaga ggcactagga ttaccccagg gccatctgtg ctccatgccc gcccatcccc
     1801 ttgccgcctg actgggtcgg atggccccag tgggtttagt cagggcttct ggattcctcg
     1861 gtttctgggc tacctatggc ttcagccttc agctcctggg agtcccagct gttgttccca
     1921 gcaacgtcgc cactgccctc ctactctcca ggctttgtca tttcaaggct gctgaaatgc
     1981 tgcatttcag gggccaccat ggagcagccg ttatttatag aactgcctgt tggaggtggg
     2041 gagtcctccc tccattcttg tccagaaaac tccttagctc tcgcagtgag ccatgttctt
     2101 agtctccagg gatggatggc cttgtatatg gacccctgag aatgagcaat tgagaaaaca
     2161 aaacaaaagg aacaatccat gaacttagat tttattggtt tcactcaaaa tgctgcagtc
     2221 atttgacctg aacttgtggc aagagacttg tgctttctaa attcaaagac tagaaggaaa
     2281 atggataaaa atcacaagtg ccgtttctct tgcaatgtag cgctattcta ctgaaatttc
     2341 tttcttctct tttctttaca aaatcataaa gaaaaaatta attcatta
//
