LOCUS       NM_002733               1657 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein kinase AMP-activated non-catalytic subunit
            gamma 1 (PRKAG1), transcript variant 1, mRNA.
ACCESSION   NM_002733
VERSION     NM_002733.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Bai,W., Guo,T., Wang,H., Li,B., Sun,Q., Wu,W., Zhang,J., Zhou,J.,
            Luo,J., Zhu,M., Lu,J., Li,P., Dong,B., Han,S., Pang,X., Zhang,G.,
            Bai,Y. and Wang,S.
  TITLE     S-nitrosylation of AMPKgamma impairs coronary collateral
            circulation and disrupts VSMC reprogramming
  JOURNAL   EMBO Rep 25 (1), 128-143 (2024)
   PUBMED   38177907
  REMARK    GeneRIF: S-nitrosylation of AMPKgamma impairs coronary collateral
            circulation and disrupts VSMC reprogramming.
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1657)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1657)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1657)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   6  (bases 1 to 1657)
  AUTHORS   Dyck,J.R., Gao,G., Widmer,J., Stapleton,D., Fernandez,C.S.,
            Kemp,B.E. and Witters,L.A.
  TITLE     Regulation of 5'-AMP-activated protein kinase activity by the
            noncatalytic beta and gamma subunits
  JOURNAL   J Biol Chem 271 (30), 17798-17803 (1996)
   PUBMED   8663446
REFERENCE   7  (bases 1 to 1657)
  AUTHORS   Woods,A., Cheung,P.C., Smith,F.C., Davison,M.D., Scott,J.,
            Beri,R.K. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase beta and gamma
            subunits. Assembly of the heterotrimeric complex in vitro
  JOURNAL   J Biol Chem 271 (17), 10282-10290 (1996)
   PUBMED   8626596
REFERENCE   8  (bases 1 to 1657)
  AUTHORS   Gao,G., Fernandez,C.S., Stapleton,D., Auster,A.S., Widmer,J.,
            Dyck,J.R., Kemp,B.E. and Witters,L.A.
  TITLE     Non-catalytic beta- and gamma-subunit isoforms of the
            5'-AMP-activated protein kinase
  JOURNAL   J Biol Chem 271 (15), 8675-8681 (1996)
   PUBMED   8621499
REFERENCE   9  (bases 1 to 1657)
  AUTHORS   Stapleton,D., Mitchelhill,K.I., Gao,G., Widmer,J., Michell,B.J.,
            Teh,T., House,C.M., Fernandez,C.S., Cox,T., Witters,L.A. and
            Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase subfamily
  JOURNAL   J Biol Chem 271 (2), 611-614 (1996)
   PUBMED   8557660
REFERENCE   10 (bases 1 to 1657)
  AUTHORS   Hofmann,B., Nishanian,P., Nguyen,T., Insixiengmay,P. and Fahey,J.L.
  TITLE     Human immunodeficiency virus proteins induce the inhibitory
            cAMP/protein kinase A pathway in normal lymphocytes
  JOURNAL   Proc Natl Acad Sci U S A 90 (14), 6676-6680 (1993)
   PUBMED   7688126
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB020277.1, BC000358.2 and
            BM667691.1.
            
            On May 17, 2019 this sequence version replaced NM_002733.4.
            
            Summary: The protein encoded by this gene is a regulatory subunit
            of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
            consisting of an alpha catalytic subunit, and non-catalytic beta
            and gamma subunits. AMPK is an important energy-sensing enzyme that
            monitors cellular energy status. In response to cellular metabolic
            stresses, AMPK is activated, and thus phosphorylates and
            inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
            beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
            regulating de novo biosynthesis of fatty acid and cholesterol. This
            subunit is one of the gamma regulatory subunits of AMPK.
            Alternatively spliced transcript variants encoding distinct
            isoforms have been observed. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.114863.1,
                                           SRR3476690.679797.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000548065.7/ ENSP00000447433.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DB020277.1         71-76
            7-1650              BC000358.2         1-1644
            1651-1657           BM667691.1         18-24               c
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.12"
     gene            1..1657
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /note="protein kinase AMP-activated non-catalytic subunit
                     gamma 1"
                     /db_xref="GeneID:5571"
                     /db_xref="HGNC:HGNC:9385"
                     /db_xref="MIM:602742"
     exon            1..45
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..1032
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /note="isoform 1 is encoded by transcript variant 1; AMPK
                     gamma-1 chain; 5'-AMP-activated protein kinase, gamma-1
                     subunit; AMPK gamma1; protein kinase, AMP-activated, gamma
                     1 non-catalytic subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit gamma-1
                     isoform 1"
                     /protein_id="NP_002724.1"
                     /db_xref="CCDS:CCDS8777.1"
                     /db_xref="GeneID:5571"
                     /db_xref="HGNC:HGNC:9385"
                     /db_xref="MIM:602742"
                     /translation="METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPT
                     SSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSA
                     LVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES
                     GNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALG
                     IFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGV
                     LKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP"
     exon            46..94
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            95..204
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            205..286
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            287..345
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            346..391
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            392..446
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            447..573
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            574..739
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            740..777
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            778..924
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1657
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1632..1637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /note="hexamer: ATTAAA"
     polyA_site      1657
                     /gene="PRKAG1"
                     /gene_synonym="AMPKG"
                     /note="major polyA site"
ORIGIN      
        1 cctaaagcga ggaaaccaag ccgggcgcct cttgcaatgg agacggtcat ttcttcagat
       61 agctccccag ctgtggaaaa tgagcatcct caagagaccc cagaatccaa caatagcgtg
      121 tatacttcct tcatgaagtc tcatcgctgc tatgacctga ttcccacaag ctccaaattg
      181 gttgtatttg atacgtccct gcaggtgaag aaagcttttt ttgctttggt gactaacggt
      241 gtacgagctg cccctttatg ggatagtaag aagcaaagtt ttgtgggcat gctgaccatc
      301 actgatttca tcaatatcct gcaccgctac tataaatcag ccttggtaca gatctatgag
      361 ctagaagaac acaagataga aacttggaga gaggtgtatc tccaggactc ctttaaaccg
      421 cttgtctgca tttctcctaa tgccagcttg tttgatgctg tctcttcatt aattcggaac
      481 aagatccaca ggctgccagt tattgaccca gaatcaggca atactttgta catcctcacc
      541 cacaagcgca ttctgaagtt cctcaaattg tttatcactg agttccccaa gccagagttc
      601 atgtccaagt ctctggaaga gctacagatt ggcacctatg ccaatattgc tatggttcgc
      661 actaccaccc ccgtctatgt ggctctgggg atttttgtac agcatcgagt ctcagccctg
      721 ccagtggtgg atgagaaggg gcgtgtggtg gacatctact ccaagtttga tgttatcaat
      781 ctggcagcag aaaagaccta caacaaccta gatgtatctg tgactaaagc cttgcaacat
      841 cgatcacatt actttgaggg tgttctcaag tgctacctgc atgagactct ggagaccatc
      901 atcaacaggc tagtggaagc agaggttcac cgacttgtag tggtggatga aaatgatgtg
      961 gtcaagggaa ttgtatcact gtctgacatc ctgcaggccc tggtgctcac aggtggagag
     1021 aagaagccct gagctggggg aaggggtcat gcagcaccag gggatatgcc caactcactg
     1081 cctgctggaa gctctgtggg aatcagatga aacttgaggg aattgtgact ctgttccctg
     1141 ttcagggtcc cctgcccttc tatctgggag ctagggaagg tatgggggag gaaagagaat
     1201 ggatttatag ctacccttac cctcacacat acacttgaaa aaactttcag cctagccagt
     1261 tctagcccct gtcctcttag atatatcccc ctttctgggt gaactatagg ctctgtgcct
     1321 ctcagacaaa ttctgatctc taagagatcc ccagacctca cttgcctctg cctccatctt
     1381 ggccctgatt caaccctaag ataatagcac aacaaaattc ttcataaaga tatttttatt
     1441 cacctgttcc gtgctatatg gaggaggcca agtccattta gtgacatttc ttcccataat
     1501 gtgagtgggg aggattgtgg ggaggagggg ctttgggttc ctgtgtttgt gcatatgaag
     1561 ggagatgggg gttaggtgga ggaggagagc agcgtggtta gctaaggtta ttgctttttg
     1621 tggcaaatct aattaaatga caggaatctc ttcacga
//
