LOCUS       NM_005399               5343 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens protein kinase AMP-activated non-catalytic subunit
            beta 2 (PRKAB2), transcript variant 1, mRNA.
ACCESSION   NM_005399
VERSION     NM_005399.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Du,H., Wang,Y., Zhu,Y., Li,X., Zhu,T., Wu,Q. and Zha,F.
  TITLE     MiR-29b Alleviates High Glucose-induced Inflammation and Apoptosis
            in Podocytes by Down-regulating PRKAB2
  JOURNAL   Endocr Metab Immune Disord Drug Targets 24 (8), 981-990 (2024)
   PUBMED   38204237
  REMARK    GeneRIF: MiR-29b Alleviates High Glucose-induced Inflammation and
            Apoptosis in Podocytes by Down-regulating PRKAB2.
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5343)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5343)
  AUTHORS   Ziegler,N., Bader,E., Epanchintsev,A., Margerie,D., Kannt,A. and
            Schmoll,D.
  TITLE     AMPKbeta1 and AMPKbeta2 define an isoform-specific gene signature
            in human pluripotent stem cells, differentially mediating cardiac
            lineage specification
  JOURNAL   J Biol Chem 295 (51), 17659-17671 (2020)
   PUBMED   33454005
  REMARK    GeneRIF: AMPKbeta1 and AMPKbeta2 define an isoform-specific gene
            signature in human pluripotent stem cells, differentially mediating
            cardiac lineage specification.
REFERENCE   5  (bases 1 to 5343)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 5343)
  AUTHORS   Thornton,C., Snowden,M.A. and Carling,D.
  TITLE     Identification of a novel AMP-activated protein kinase beta subunit
            isoform that is highly expressed in skeletal muscle
  JOURNAL   J Biol Chem 273 (20), 12443-12450 (1998)
   PUBMED   9575201
REFERENCE   7  (bases 1 to 5343)
  AUTHORS   Stapleton,D., Woollatt,E., Mitchelhill,K.I., Nicholl,J.K.,
            Fernandez,C.S., Michell,B.J., Witters,L.A., Power,D.A.,
            Sutherland,G.R. and Kemp,B.E.
  TITLE     AMP-activated protein kinase isoenzyme family: subunit structure
            and chromosomal location
  JOURNAL   FEBS Lett 409 (3), 452-456 (1997)
   PUBMED   9224708
REFERENCE   8  (bases 1 to 5343)
  AUTHORS   Woods,A., Cheung,P.C., Smith,F.C., Davison,M.D., Scott,J.,
            Beri,R.K. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase beta and gamma
            subunits. Assembly of the heterotrimeric complex in vitro
  JOURNAL   J Biol Chem 271 (17), 10282-10290 (1996)
   PUBMED   8626596
REFERENCE   9  (bases 1 to 5343)
  AUTHORS   Gao,G., Fernandez,C.S., Stapleton,D., Auster,A.S., Widmer,J.,
            Dyck,J.R., Kemp,B.E. and Witters,L.A.
  TITLE     Non-catalytic beta- and gamma-subunit isoforms of the
            5'-AMP-activated protein kinase
  JOURNAL   J Biol Chem 271 (15), 8675-8681 (1996)
   PUBMED   8621499
REFERENCE   10 (bases 1 to 5343)
  AUTHORS   Stapleton,D., Mitchelhill,K.I., Gao,G., Widmer,J., Michell,B.J.,
            Teh,T., House,C.M., Fernandez,C.S., Cox,T., Witters,L.A. and
            Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase subfamily
  JOURNAL   J Biol Chem 271 (2), 611-614 (1996)
   PUBMED   8557660
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC053610.1, AC241584.3 and
            CA311731.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005399.4.
            
            Summary: The protein encoded by this gene is a regulatory subunit
            of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
            consisting of an alpha catalytic subunit, and non-catalytic beta
            and gamma subunits. AMPK is an important energy-sensing enzyme that
            monitors cellular energy status. In response to cellular metabolic
            stresses, AMPK is activated, and thus phosphorylates and
            inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
            beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
            regulating de novo biosynthesis of fatty acid and cholesterol. This
            subunit may be a positive regulator of AMPK activity. It is highly
            expressed in skeletal muscle and thus may have tissue-specific
            roles. Multiple alternatively spliced transcript variants have been
            found for this gene. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes a functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292820.1, BC053610.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254101.4/ ENSP00000254101.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1111              BC053610.1         75-1185
            1112-4692           AC241584.3         165719-169299       c
            4693-5343           CA311731.1         14-664              c
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.1"
     gene            1..5343
                     /gene="PRKAB2"
                     /note="protein kinase AMP-activated non-catalytic subunit
                     beta 2"
                     /db_xref="GeneID:5565"
                     /db_xref="HGNC:HGNC:9379"
                     /db_xref="MIM:602741"
     exon            1..42
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            43..221
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..884
                     /gene="PRKAB2"
                     /note="AMPK beta 2; AMPK beta-2 chain; 5'-AMP-activated
                     protein kinase, beta-2 subunit; protein kinase,
                     AMP-activated, beta 2 non-catalytic subunit; AMPK subunit
                     beta-2; AMP-activated protein kinase beta 2 non-catalytic
                     subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit beta-2"
                     /protein_id="NP_005390.1"
                     /db_xref="CCDS:CCDS925.1"
                     /db_xref="GeneID:5565"
                     /db_xref="HGNC:HGNC:9379"
                     /db_xref="MIM:602741"
                     /translation="MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPS
                     VFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTK
                     IPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDF
                     EVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILN
                     KDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI"
     exon            222..388
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            389..482
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            483..603
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            604..737
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            738..806
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     exon            807..5343
                     /gene="PRKAB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5320..5325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAB2"
                     /note="hexamer: AATAAA"
     polyA_site      5343
                     /gene="PRKAB2"
                     /note="major polyA site"
ORIGIN      
        1 atcacatcag tggcccttgg aggagggagc gcatcgcccg aggtggtccc cgacgagctg
       61 cagccatggg aaacaccacc agcgaccggg tgtccgggga gcgccacggc gccaaggctg
      121 cacgctccga gggcgcaggc ggccatgccc cggggaagga gcacaagatc atggtgggga
      181 gtacggacga ccccagcgtg ttcagcctcc ctgactccaa gctccctggg gacaaagagt
      241 ttgtatcatg gcagcaggat ttggaggact ccgtaaagcc cacacagcag gcccggccca
      301 ctgttatccg ctggtctgaa ggaggcaagg aggtcttcat ctctgggtcc ttcaacaatt
      361 ggagcaccaa gattccactg attaagagcc ataatgactt tgttgccatc ctggacctcc
      421 ctgagggaga gcaccaatac aagttctttg tggatggaca gtgggttcat gatccatcag
      481 agcctgtggt taccagtcag cttggcacaa ttaacaattt gatccatgtc aagaaatctg
      541 attttgaggt gttcgatgct ttaaagttag attctatgga aagttctgag acatcttgta
      601 gagacctttc cagctcaccc ccagggcctt atggtcaaga aatgtatgcg tttcgatctg
      661 aggaaagatt caaatcccca cccatccttc ctcctcatct acttcaagtt attcttaaca
      721 aagacactaa tatttcttgt gacccagcct tactccctga gcccaaccat gttatgctga
      781 accatctcta tgcattgtcc attaaggaca gtgtgatggt ccttagcgca acccatcgct
      841 acaagaagaa gtatgttact actctgctat acaagcccat ttgaagggat cccttcttgc
      901 ctctaaggat tcaggagaag catctccctt gcatttctgg actgaaccag tcttacctga
      961 gactggaagg ctgatttgct ttgaggctga tatgtgtgtt tcagagcctc tgagtaggat
     1021 gctctgcttt tgcatttgat tgcagatgag agctttatga gttcacggaa tttattttaa
     1081 gaaaaaaaaa tatacatatg agaagaaggt aaatggaagc ctcctagccc cagctagaag
     1141 tattgtttct gcctgtgggt tttcaccaag acctgtttgg gggcgctgca ggaataacta
     1201 tataggaaga tttttcctaa aatgaaagaa cagcaaactc ttaggatcct tgttgggtgg
     1261 agattctatc actgctacct tggctctcca aggaatgggc ttgtgctaga ccgctgccct
     1321 acttaacagc tgcctcattg caagggcagt ttttcttgca tgggttctct atattcccag
     1381 agtatgtggc acaatctgtg ttgtttatat gataccagat gccccacaag aacccttatt
     1441 cctctcattt cacattcttc ctttaatagc ctccttcaga tcccatacct gacccctctc
     1501 taacacaaaa cttattgggt aagtgacttt gaaaagtttt gtggcacctg acccacccca
     1561 gacactaggg ctatcagaag gtctcctttt tagcccagca caggcccagg ccactttgtc
     1621 gtgtttgttt taacttctaa agaaaatatg tttcagcatt ataagaaagg cagaatgcag
     1681 aacacctaca tttttgtttt agtttggtgc caaggctcag gctgtattgg caaattcccg
     1741 aaagttttcc cactttgcct ggccctgctt ctgtcttttc tttctcagta aacagttctg
     1801 aaggcaggag tggaacccgg gagtattttc atgtctttca tccttgaaag atttttatgt
     1861 gcctgcattt tttttttaat taaaaaatgc cttttcattg gtcttaagag accgcattgg
     1921 agaatttcag gcttttgata aatgcttctt caaagagatt ttcttctcta gtctagcctt
     1981 ccacattctt agattaatat ggccaaccct gtacacatca ctacactaaa cactgctcta
     2041 gataaactgc tcaagttcat ttaactcatt tgatgcacct aaaggggttc ctcattttaa
     2101 agatttgtta ggccaagaag caagagagta ttcctagtat tcccaaccat gaaaagtatc
     2161 attctttgca ccaaatgtta acaaaatcat tttgttctcc tgcctcttct ttttaaaggt
     2221 gtttgatgat taagtggggt cactgaattc catttgtgga ctgaaaagta ttcaatccac
     2281 ttttggggtt cagagataaa acattttttc ccaagtagct ggggctcttc cattttgcag
     2341 ataagtcaaa taatcaacac taaaggaggc taaactgttg atgaatgaga gactccctga
     2401 ctgctcagat gaccctagcc acactgaaag ggcacctaca ggtcagttta gctacctcct
     2461 gtctttccca tgcaaagctg ataacacagt tgtctttgga cttgtagacc tcttggattc
     2521 caggtgtgat ggagtaaagt gtgggattgt tgttttgctg ggatgcaaat aactaaatgc
     2581 tttggtggtt aattgctaag agtaaatact actttagcca tccaaggcca ccttctgcag
     2641 caaaaggctt ttgtggagaa ccttttatgt tcccaaccac tttttgaatg gtgtgccatt
     2701 taaaaatcca ggccagatcc tattataacc aactctcagg atttacagcc ttcagttgta
     2761 ctagaatttt gtttttatcc aatactcatt aaataagtgg gccacttagg aagattcaaa
     2821 atcttggtta ttacatgaag tttgttatat ttcttgtcaa cagtattgaa atgtaatatg
     2881 tatgtgttca tgtatgaaaa tttttactcc acacaggtgt ttcagtagag tggggcagga
     2941 aaagagatct cttcgatttc tttcaggcct gaggcttttg tgaaatgcgt cagccccctg
     3001 tgacagtagg ttttgatgct agtgatcttc agatctttct ctctggaaat gtgcagagag
     3061 tgtcagtttc ccaagttctg aggtaactct cagcccagat gtgaaatggg agcctaccag
     3121 ctggtataga agggaatggg taggaggcac tgggtgctga ctcattcagc actgtccctt
     3181 ttctatactg ctgatacatc ccatggttct gagaagcctt atctcagtct atttggaaga
     3241 gagggaggaa gagaaggaag taacccaaag tactactcat ttatcattgt atattgatta
     3301 gttaaaggga taattaattt aatgctgagg agagtttgac agattttgaa aatgagtaaa
     3361 ggcaaaaaaa atttttttag cctttatttt gcttttggga attttacaga gtcaaagtag
     3421 gcagaataag aaaatagttc ttcaggaggg ccgaccttta aagaacttca acatagtttc
     3481 ggaattgtgg ggaagagaag agtgactgag ctgagaagta ataatagaat aaagggttga
     3541 gtaacttaca actgaaaatg atctctttta aaaagaaatt aaatcagaca ccacatggtg
     3601 gtgtccttgg atctcactgt acagaattag cagtgtataa ccatcttctc ttttcatctt
     3661 gttccaattc tctcctcttt cctttccatt ctgctttaag ctcatgtgtc aggcagactt
     3721 taccagagtg tcagacatta cctaaaacac atacgttagc catgctgctg gtatggagaa
     3781 attccacacc atgattatta gcctccttta agctgaatgg gatttaacca ttctaggcaa
     3841 cacccctgaa gggcatacct aacctcaata gtgttggctt ttaaaacgta tgtttgtatg
     3901 gtagagaaac tttgtaaaag aagaatccaa gagaagtttg tgaggatcct acaaacccag
     3961 gcccactcac tttgctctaa ttctttctag tatcttgtag atctaatggg tctgggataa
     4021 aaactttgaa aagtgtcaat attccatgta tgctgctgaa atgaagttaa gtttggaaag
     4081 aagtgatacc tctagactgg gtttatatta atctgggata taaatgaaga agacatacta
     4141 atagaactcc ttgcttttaa ttggggaaat agggctttaa taattttgac ctcaactaaa
     4201 aatgatatgc aatagtctct gtgtgtgttt gaaatacatt gtgttctcag agatttctac
     4261 attctcacgt tctagtgatt tggggcatgg gcttaatagc agatgtacag tgtattcctg
     4321 cattattgtg attcccctta aagcccagtt cttgctgtct tctaccaggg gctgctgact
     4381 ccagttaccc atggaatgca ggacctggga ggggtagcca ttagggtctt tcaaaactct
     4441 ttggatctaa gcatttgtct ctccttaagt gccaatcaca attggatatg gaaggactgt
     4501 gatttctgca atgaacccaa acttttagag taaaaagcca aatttaaatt ataagaaaga
     4561 agggaaaaaa gagaaaaact caagtctatt acttgtagag tccaattctt agcaatggaa
     4621 tcgctctagg attctagttt gggctttgtc tggatttgct tttctcagtt gtgctttgaa
     4681 gtgaataagc tttgttacaa attaattttt tattagttcc aatattagtt ggagttaact
     4741 tgaattgatt gtatgtagca cagcactttt gcagtaagat tggtgtgaaa tactaaacac
     4801 tatggatttt gtaggtgtca ggttaaatgg tcaagggata cctacattaa gtcatatatt
     4861 aggtattgat gatcttactt cttttctgtt cccctgtaca aaacacttac ctaacccagc
     4921 ttgtggtttt aggacagcca aagctcactg ttgttggtta gtcctaatca ctacacgggt
     4981 ctcataaatg agacttgttt gaattttggt acattggagc atgttggttg gtattacacg
     5041 gcagcatttc gaatgagtgc agctctgtgt ctgtcagaaa ggagagataa gactactttg
     5101 aagggaatta aatatgtgag tcctcttttt aatggtgctt tttgtaacct ttaatgctga
     5161 ggtacagagc tgcttttcaa tatttcataa aggagtggca gacaagagtg gattttaaag
     5221 ctgttcttca aacgtaattt gtcactggac tctgacacac ctggaaatta tatgatatga
     5281 tacatacaga aatgttgtgg gttttttcca taaaacttta ataaaagtat tatacagcaa
     5341 taa
//
