LOCUS       NM_006253               2219 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens protein kinase AMP-activated non-catalytic subunit
            beta 1 (PRKAB1), mRNA.
ACCESSION   NM_006253
VERSION     NM_006253.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Chen,X., Wang,H., Wu,C., Li,X., Huang,X., Ren,Y., Pu,Q., Cao,Z.,
            Tang,X. and Ding,B.S.
  TITLE     Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor
            PAI-1 and contributes to lung fibrosis
  JOURNAL   Redox Biol 70, 103038 (2024)
   PUBMED   38266576
  REMARK    GeneRIF: Endothelial H2S-AMPK dysfunction upregulates the
            angiocrine factor PAI-1 and contributes to lung fibrosis.
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Duan,P., Liu,Y., Lin,X., Ren,J., He,J., Liu,X. and Xie,J.
  TITLE     [Extracellular Matrix Stiffness Induces Mitochondrial Morphological
            Heterogeneity via AMPK Activation]
  JOURNAL   Sichuan Da Xue Xue Bao Yi Xue Ban 55 (1), 47-52 (2024)
   PUBMED   38322520
  REMARK    GeneRIF: [Extracellular Matrix Stiffness Induces Mitochondrial
            Morphological Heterogeneity via AMPK Activation].
REFERENCE   3  (bases 1 to 2219)
  AUTHORS   Ding,Y., Lv,Z., Cao,W., Shi,W., He,Q. and Gao,K.
  TITLE     Phosphorylation of INF2 by AMPK promotes mitochondrial fission and
            oncogenic function in endometrial cancer
  JOURNAL   Cell Death Dis 15 (1), 65 (2024)
   PUBMED   38233384
  REMARK    GeneRIF: Phosphorylation of INF2 by AMPK promotes mitochondrial
            fission and oncogenic function in endometrial cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2219)
  AUTHORS   Rahman,A.A., Butcko,A.J., Songyekutu,E., Granneman,J.G. and
            Mottillo,E.P.
  TITLE     Direct effects of adipocyte lipolysis on AMPK through intracellular
            long-chain acyl-CoA signaling
  JOURNAL   Sci Rep 14 (1), 19 (2024)
   PUBMED   38167670
  REMARK    GeneRIF: Direct effects of adipocyte lipolysis on AMPK through
            intracellular long-chain acyl-CoA signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2219)
  AUTHORS   Crosas-Molist,E., Graziani,V., Maiques,O., Pandya,P., Monger,J.,
            Samain,R., George,S.L., Malik,S., Salise,J., Morales,V., Le
            Guennec,A., Atkinson,R.A., Marti,R.M., Matias-Guiu,X., Charras,G.,
            Conte,M.R., Elosegui-Artola,A., Holt,M. and Sanz-Moreno,V.
  TITLE     AMPK is a mechano-metabolic sensor linking cell adhesion and
            mitochondrial dynamics to Myosin-dependent cell migration
  JOURNAL   Nat Commun 14 (1), 2740 (2023)
   PUBMED   37217519
  REMARK    GeneRIF: AMPK is a mechano-metabolic sensor linking cell adhesion
            and mitochondrial dynamics to Myosin-dependent cell migration.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2219)
  AUTHORS   Stapleton,D., Woollatt,E., Mitchelhill,K.I., Nicholl,J.K.,
            Fernandez,C.S., Michell,B.J., Witters,L.A., Power,D.A.,
            Sutherland,G.R. and Kemp,B.E.
  TITLE     AMP-activated protein kinase isoenzyme family: subunit structure
            and chromosomal location
  JOURNAL   FEBS Lett 409 (3), 452-456 (1997)
   PUBMED   9224708
REFERENCE   7  (bases 1 to 2219)
  AUTHORS   Dyck,J.R., Gao,G., Widmer,J., Stapleton,D., Fernandez,C.S.,
            Kemp,B.E. and Witters,L.A.
  TITLE     Regulation of 5'-AMP-activated protein kinase activity by the
            noncatalytic beta and gamma subunits
  JOURNAL   J Biol Chem 271 (30), 17798-17803 (1996)
   PUBMED   8663446
REFERENCE   8  (bases 1 to 2219)
  AUTHORS   Woods,A., Cheung,P.C., Smith,F.C., Davison,M.D., Scott,J.,
            Beri,R.K. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase beta and gamma
            subunits. Assembly of the heterotrimeric complex in vitro
  JOURNAL   J Biol Chem 271 (17), 10282-10290 (1996)
   PUBMED   8626596
REFERENCE   9  (bases 1 to 2219)
  AUTHORS   Gao,G., Fernandez,C.S., Stapleton,D., Auster,A.S., Widmer,J.,
            Dyck,J.R., Kemp,B.E. and Witters,L.A.
  TITLE     Non-catalytic beta- and gamma-subunit isoforms of the
            5'-AMP-activated protein kinase
  JOURNAL   J Biol Chem 271 (15), 8675-8681 (1996)
   PUBMED   8621499
REFERENCE   10 (bases 1 to 2219)
  AUTHORS   Stapleton,D., Mitchelhill,K.I., Gao,G., Widmer,J., Michell,B.J.,
            Teh,T., House,C.M., Fernandez,C.S., Cox,T., Witters,L.A. and
            Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase subfamily
  JOURNAL   J Biol Chem 271 (2), 611-614 (1996)
   PUBMED   8557660
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU539177.1, BC017671.1 and
            AF022116.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006253.4.
            
            Summary: The protein encoded by this gene is a regulatory subunit
            of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
            consisting of an alpha catalytic subunit, and non-catalytic beta
            and gamma subunits. AMPK is an important energy-sensing enzyme that
            monitors cellular energy status. In response to cellular metabolic
            stresses, AMPK is activated, and thus phosphorylates and
            inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
            beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
            regulating de novo biosynthesis of fatty acid and cholesterol. This
            subunit may be a positive regulator of AMPK activity. The
            myristoylation and phosphorylation of this subunit have been shown
            to affect the enzyme activity and cellular localization of AMPK.
            This subunit may also serve as an adaptor molecule mediating the
            association of the AMPK complex. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017671.1, AF022116.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229328.10/ ENSP00000229328.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-529               BU539177.1         179-707
            530-1977            BC017671.1         693-2140
            1978-2219           AF022116.1         2133-2374
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.23"
     gene            1..2219
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /note="protein kinase AMP-activated non-catalytic subunit
                     beta 1"
                     /db_xref="GeneID:5564"
                     /db_xref="HGNC:HGNC:9378"
                     /db_xref="MIM:602740"
     exon            1..271
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..925
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /note="AMPK beta 1; AMP-activated protein kinase beta
                     subunit; protein kinase, AMP-activated, beta 1
                     non-catalytic subunit; protein kinase, AMP-activated,
                     noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated
                     protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase subunit beta-1"
                     /protein_id="NP_006244.2"
                     /db_xref="CCDS:CCDS9191.1"
                     /db_xref="GeneID:5564"
                     /db_xref="HGNC:HGNC:9378"
                     /db_xref="MIM:602740"
                     /translation="MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDA
                     DLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSK
                     LPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDF
                     EVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKD
                     TGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI"
     exon            272..435
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     exon            436..529
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     exon            530..644
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     exon            645..778
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     exon            779..847
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     exon            848..2219
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2199..2204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /note="hexamer: AATAAA"
     polyA_site      2219
                     /gene="PRKAB1"
                     /gene_synonym="AMPK; HAMPKb"
                     /note="major polyA site"
ORIGIN      
        1 gactccttcc gcaggctcct tgggacccgc ggttccggga gtcccttgct cagggtccct
       61 ttcctgcagt gaggcgccgt ccgccttccc tgtgtccccg cagaccccca tcatgggcaa
      121 taccagcagt gagcgcgccg cgctggagcg gcatggtggc cataagacgc cccggaggga
      181 cagctcgggg ggcaccaagg acggggacag gcccaagatc ctgatggaca gccccgaaga
      241 cgccgacctc ttccactccg aggaaatcaa ggcaccagag aaggaggaat tcctggcctg
      301 gcagcatgat ctggaagtga atgataaagc tcccgcccag gctcggccaa cggtgtttcg
      361 atggacgggg ggcggaaagg aagtttactt atctgggtcc ttcaacaact ggagtaaact
      421 tcccctcacc agaagccaca ataactttgt agccatcctg gatctgccgg aaggagagca
      481 tcagtacaag ttctttgtgg atggtcagtg gacgcacgac ccttccgagc ccatagtaac
      541 cagccagctt ggcacagtta acaacatcat tcaagtgaag aaaactgact ttgaagtatt
      601 tgatgcttta atggtggatt cccaaaagtg ctccgatgtg tctgagctgt ccagttctcc
      661 cccaggaccc taccatcagg agccctacgt ctgcaaaccc gaagagcgct ttcgggcacc
      721 ccctattctc cccccacatc tcctccaggt catcctgaac aaggacacgg ggatttcctg
      781 tgatccagct ttgcttcctg agcccaatca cgtcatgctg aaccacctat acgcgctgtc
      841 tatcaaggat ggagtgatgg tgctcagcgc aacccaccgg tacaagaaga agtacgtcac
      901 caccttgtta tacaagccca tatgaagagc tgggggcgga tggtggccca ggagacagca
      961 caccaccagg ctccacacgt gcatgctttc cccaagaggg aatggactgt acattgctca
     1021 tttcacactc ttcagaagac atttcatacc tgccctggtc ctgcttgaag gtttgtccag
     1081 gcagagcagc tcctgcagcg cctcggtctg tgacagtcct cctagcaccc ccatggcttt
     1141 gagcctcggg gactcatcaa gtccaagaaa agagggaggg gtggcagagg atctgcagcc
     1201 ctggccccgc ggtgcatgag gctgggtgca gttctaaacc tacattctcg atttttctta
     1261 agccaaaaat gaatgctaac tcctttgcca gtaaaattct gggaaacagg gactgaggcc
     1321 acacatcatt tccagtcatc tgtgtgtttt taaggccagc cacttgtccc tgttgaggcc
     1381 tggctatgga actaaataca gtgttggtct tgcctgtcct tcaaaatcaa caacagattg
     1441 tctctcggct ccagggaggt gtcatttcta tagaaattag aagctttctg atttctagat
     1501 gaggttttac aattgtttct tacagtcatg tgcactaagt actctttttg taagcagagg
     1561 tggctggctc tgcagcctta aggccatttt ttaagtcacc acgtctagaa gtcacatgaa
     1621 ctctgctcag caataatctg ttctcagaac agacttttca acctgctgcc ggatttctcc
     1681 attcagctgg atgatcctca ggactgacca gttagctggc aggttgtcca gctttttatt
     1741 ccagtcataa taggtgacag tgttaaccgt gaaaacttga gaggcactct gccctcttcc
     1801 ctataaaatc acacagcgtg attttacaag gtcccgtggc accttgctca ggacctctgc
     1861 ccctagttag caagactgca gcagttgctg ttgcttattc tgaaaggaat gtagaacttg
     1921 acagcagcct tctgagtctg ggtcaggaag atgtcctttg gaccaaagca gacttcttta
     1981 tacgcagctc agtttcccgg gagtcgccac agatgtaccc actagcccag gttgctgtga
     2041 gtcagcggaa gctcccgtta tgccctttgc tcctggtggg agagggagga gtgagctccc
     2101 tgggttccag tatttacttg gtatacctga gtttgggggt accctttttt gtgacttttc
     2161 aaaacagtga attactgtca ccttgatgga caagtttcaa taaaactttg taaaaataa
//
