LOCUS       NM_006252               9355 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2
            (PRKAA2), mRNA.
ACCESSION   NM_006252
VERSION     NM_006252.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Ren,F., Yang,M., Liu,G., Qi,Y., Li,A., Li,J. and Zheng,L.
  TITLE     SIRT5-mediated PRKAA2 succinylation ameliorates apoptosis of human
            placental trophoblasts in hypertensive disorder complicating
            pregnancy
  JOURNAL   Clin Exp Hypertens 46 (1), 2358030 (2024)
   PUBMED   38785262
  REMARK    GeneRIF: SIRT5-mediated PRKAA2 succinylation ameliorates apoptosis
            of human placental trophoblasts in hypertensive disorder
            complicating pregnancy.
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Ouyang,Y., Gu,Y., Zhang,X., Huang,Y., Wei,X., Tang,F. and Zhang,S.
  TITLE     AMPKalpha2 promotes tumor immune escape by inducing CD8+ T-cell
            exhaustion and CD4+ Treg cell formation in liver hepatocellular
            carcinoma
  JOURNAL   BMC Cancer 24 (1), 276 (2024)
   PUBMED   38424484
  REMARK    GeneRIF: AMPKalpha2 promotes tumor immune escape by inducing CD8+
            T-cell exhaustion and CD4+ Treg cell formation in liver
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9355)
  AUTHORS   Asanoma,K., Yagi,H., Onoyama,I., Cui,L., Hori,E., Kawakami,M.,
            Maenohara,S., Hachisuga,K., Tomonobe,H., Kodama,K., Yasunaga,M.,
            Ohgami,T., Okugawa,K., Yahata,H., Kitao,H. and Kato,K.
  TITLE     The BHLHE40-PPM1F-AMPK pathway regulates energy metabolism and is
            associated with the aggressiveness of endometrial cancer
  JOURNAL   J Biol Chem 300 (3), 105695 (2024)
   PUBMED   38301894
  REMARK    GeneRIF: The BHLHE40-PPM1F-AMPK pathway regulates energy metabolism
            and is associated with the aggressiveness of endometrial cancer.
REFERENCE   4  (bases 1 to 9355)
  AUTHORS   Ross,F.A., Hawley,S.A., Russell,F.M., Goodman,N. and Hardie,D.G.
  TITLE     Frequent loss-of-function mutations in the AMPK-alpha2 catalytic
            subunit suggest a tumour suppressor role in human skin cancers
  JOURNAL   Biochem J 480 (23), 1951-1968 (2023)
   PUBMED   37962491
  REMARK    GeneRIF: Frequent loss-of-function mutations in the AMPK-alpha2
            catalytic subunit suggest a tumour suppressor role in human skin
            cancers.
REFERENCE   5  (bases 1 to 9355)
  AUTHORS   Assar,S., Dastbaz,M., Amini,K., Roghani,S.A., Lotfi,R.,
            Taghadosi,M., Kafi,H., Abdan,Z., Allahyari,H., Rostampour,R. and
            Shahrokhvand,S.Z.
  TITLE     Assessing the gene expression of the adenosine
            5'-monophosphate-activated protein kinase (AMPK) and its relation
            with the IL-6 and IL-10 plasma levels in COVID-19 patients
  JOURNAL   Mol Biol Rep 50 (12), 9925-9933 (2023)
   PUBMED   37874507
  REMARK    GeneRIF: Assessing the gene expression of the adenosine
            5'-monophosphate-activated protein kinase (AMPK) and its relation
            with the IL-6 and IL-10 plasma levels in COVID-19 patients.
REFERENCE   6  (bases 1 to 9355)
  AUTHORS   Vavvas,D., Apazidis,A., Saha,A.K., Gamble,J., Patel,A., Kemp,B.E.,
            Witters,L.A. and Ruderman,N.B.
  TITLE     Contraction-induced changes in acetyl-CoA carboxylase and
            5'-AMP-activated kinase in skeletal muscle
  JOURNAL   J Biol Chem 272 (20), 13255-13261 (1997)
   PUBMED   9148944
REFERENCE   7  (bases 1 to 9355)
  AUTHORS   Beri,R.K., Marley,A.E., See,C.G., Sopwith,W.F., Aguan,K.,
            Carling,D., Scott,J. and Carey,F.
  TITLE     Molecular cloning, expression and chromosomal localisation of human
            AMP-activated protein kinase
  JOURNAL   FEBS Lett 356 (1), 117-121 (1994)
   PUBMED   7988703
REFERENCE   8  (bases 1 to 9355)
  AUTHORS   Aguan,K., Scott,J., See,C.G. and Sarkar,N.H.
  TITLE     Characterization and chromosomal localization of the human
            homologue of a rat AMP-activated protein kinase-encoding gene: a
            major regulator of lipid metabolism in mammals
  JOURNAL   Gene 149 (2), 345-350 (1994)
   PUBMED   7959015
REFERENCE   9  (bases 1 to 9355)
  AUTHORS   Hardie,D.G. and MacKintosh,R.W.
  TITLE     AMP-activated protein kinase--an archetypal protein kinase cascade?
  JOURNAL   Bioessays 14 (10), 699-704 (1992)
   PUBMED   1365882
  REMARK    Review article
REFERENCE   10 (bases 1 to 9355)
  AUTHORS   Hardie,D.G.
  TITLE     Regulation of fatty acid and cholesterol metabolism by the
            AMP-activated protein kinase
  JOURNAL   Biochim Biophys Acta 1123 (3), 231-238 (1992)
   PUBMED   1536860
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099789.2, DA710012.1, BC069740.1, AL035705.22 and BM979925.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006252.3.
            
            Summary: The protein encoded by this gene is a catalytic subunit of
            the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
            consisting of an alpha catalytic subunit, and non-catalytic beta
            and gamma subunits. AMPK is an important energy-sensing enzyme that
            monitors cellular energy status. In response to cellular metabolic
            stresses, AMPK is activated, and thus phosphorylates and
            inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
            beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
            regulating de novo biosynthesis of fatty acid and cholesterol.
            Studies of the mouse counterpart suggest that this catalytic
            subunit may control whole-body insulin sensitivity and is necessary
            for maintaining myocardial energy homeostasis during ischemia.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because transcript sequence consistent with
            the reference genome assembly was not available for all regions of
            the RefSeq transcript. The extent of this transcript is supported
            by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.173171.1,
                                           SRR1803616.12773.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371244.9/ ENSP00000360290.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC099789.2         93907-93909
            4-424               DA710012.1         1-421
            425-2148            BC069740.1         395-2118
            2149-9214           AL035705.22        68883-75948         c
            9215-9355           BM979925.1         18-158              c
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.2"
     gene            1..9355
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="protein kinase AMP-activated catalytic subunit
                     alpha 2"
                     /db_xref="GeneID:5563"
                     /db_xref="HGNC:HGNC:9377"
                     /db_xref="MIM:600497"
     exon            1..168
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1733
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.31"
                     /note="AMPK-alpha-2 chain; 5'-AMP-activated protein
                     kinase, catalytic alpha-2 chain; AMPK subunit alpha-2;
                     ACACA kinase; HMGCR kinase; acetyl-CoA carboxylase kinase;
                     hydroxymethylglutaryl-CoA reductase kinase; AMPK alpha 2;
                     protein kinase, AMP-activated, alpha 2 catalytic subunit"
                     /codon_start=1
                     /product="5'-AMP-activated protein kinase catalytic
                     subunit alpha-2"
                     /protein_id="NP_006243.2"
                     /db_xref="CCDS:CCDS605.1"
                     /db_xref="GeneID:5563"
                     /db_xref="HGNC:HGNC:9377"
                     /db_xref="MIM:600497"
                     /translation="MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAV
                     KILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELF
                     DYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGL
                     SNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEH
                     VPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYL
                     FPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIM
                     NQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKP
                     KSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNY
                     VKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAE
                     SHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR"
     exon            169..310
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            311..404
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            405..549
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            550..637
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            638..862
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1367
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1494
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..9355
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8817..8822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="hexamer: AATAAA"
     polyA_site      8836
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="major polyA site"
     regulatory      9085..9090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="hexamer: AATAAA"
     polyA_site      9107
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
     regulatory      9326..9331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="hexamer: ATTAAA"
     polyA_site      9355
                     /gene="PRKAA2"
                     /gene_synonym="AMPK; AMPK2; AMPKa2; PRKAA"
                     /note="major polyA site"
ORIGIN      
        1 gcactgtggg taggcggcgg cggcggcggc tacgcggagc ggcaggcggt ggagcgaggc
       61 cgcgcgcgcc gaagatggct gagaagcaga agcacgacgg gcgggtgaag atcggacact
      121 acgtgctggg cgacacgctg ggcgtcggca ccttcggcaa agtgaagatt ggagaacatc
      181 aattaacagg ccataaagtg gcagttaaaa tcttaaatag acagaagatt cgcagtttag
      241 atgttgttgg aaaaataaaa cgagaaattc aaaatctaaa actctttcgt catcctcata
      301 ttatcaaact ataccaggtg atcagcactc caacagattt ttttatggta atggaatatg
      361 tgtctggagg tgaattattt gactacatct gtaagcatgg acgggttgaa gagatggaag
      421 ccaggcggct ctttcagcag attctgtctg ctgtggatta ctgtcatagg catatggttg
      481 ttcatcgaga cctgaaacca gagaatgtcc tgttggatgc acacatgaat gccaagatag
      541 ccgatttcgg attatctaat atgatgtcag atggtgaatt tctgagaact agttgcggat
      601 ctccaaatta tgcagcacct gaagtcatct caggcagatt gtatgcaggt cctgaagttg
      661 atatctggag ctgtggtgtt atcttgtatg ctcttctttg tggcaccctc ccatttgatg
      721 atgagcatgt acctacgtta tttaagaaga tccgaggggg tgtcttttat atcccagaat
      781 atctcaatcg ttctgtcgcc actctcctga tgcatatgct gcaggttgac ccactgaaac
      841 gagcaactat caaagacata agagagcatg aatggtttaa acaagatttg cccagttact
      901 tatttcctga agacccttcc tatgatgcta acgtcattga tgatgaggct gtgaaagaag
      961 tgtgtgaaaa atttgaatgt acagaatcag aagtaatgaa cagtttatat agtggtgacc
     1021 ctcaagacca gcttgcagtg gcttatcatc ttatcattga caatcggaga ataatgaacc
     1081 aagccagtga gttctacctc gcctctagtc ctccatctgg ttcttttatg gatgatagtg
     1141 ccatgcatat tcccccaggc ctgaaacctc atccagaaag gatgccacct cttatagcag
     1201 acagccccaa agcaagatgt ccattggatg cactgaatac gactaagccc aaatctttag
     1261 ctgtgaaaaa agccaagtgg catcttggaa tccgaagtca gagcaaaccg tatgacatta
     1321 tggctgaagt ttaccgagct atgaagcagc tggattttga atggaaggta gtgaatgcat
     1381 accatcttcg tgtaagaaga aaaaatccag tgactggcaa ttacgtgaaa atgagcttac
     1441 aactttacct ggttgataac aggagctatc ttttggactt taaaagcatt gatgatgaag
     1501 tagtggagca gagatctggt tcctcaacac ctcagcgttc ctgttctgct gctggcttac
     1561 acagaccaag atcaagtttt gattccacaa ctgcagagag ccattcactt tctggctctc
     1621 tcactggctc tttgaccgga agcacattgt cttcagtttc acctcgcctg ggcagtcaca
     1681 ccatggattt ttttgaaatg tgtgccagtc tgattactac tttagcccgt tgatctgtct
     1741 ctagtttctt tctgttattg cactatgaaa atcagttata ttctttaaat ttttatctta
     1801 cttttggata atatccactg caatactaat tgagaaacat gaattatttc caggggcaca
     1861 caatgctatt gaaattactg aaaacaaaat atctgacatc ttatttactt gtagaaatct
     1921 gtaattctat tgtgcctatg ataaattcac ataggcaata tctttaatag gttaatatca
     1981 atgaagattt ttaattacaa taatgagttc actacagacg attaacacac cacactggcg
     2041 aaccatctca atgtaagggt ggtttggcaa cacctccttg ctttgctgtt tggtgtaggt
     2101 aaatctagtt tacttcctaa atttcagtag gctttatgct gtgtttatgc ccccaattta
     2161 ttttaacaaa agaagattaa aaagtaaaag aaccacgagt aagatattat ttaaatgttg
     2221 aaatcttaaa aacctgcctc caagatttca gaagccaagt ttttctaaca gtatttgtac
     2281 aaatactgcc tagtgtattc aacagaagga ctgtggtcat gtaacaggta accacaattt
     2341 tcaggtttct taaaaacagc tgtaactaac tcaggatttt tatcttgaga tttccctgaa
     2401 taatatattt atcttaagag ccttcaagtt tcaaattaat attggaacat ctggaattgc
     2461 aacaactttt gtcttttaca taaacttacg tcatttaaaa aatgtcttca aaatctacct
     2521 ttctcaaatt ctttttgcct ctatttattt ttgcatttca ccaacagtga taaaatagtt
     2581 aaatgaaaca aagcaaagta tcaacagtcc cttaaatgag aatccttatc tttgatcttt
     2641 attttctgtg ttaggtgtta gggtcctggt gcagctcata atgctaattc ttcattggaa
     2701 gccactccct tcacctcacc tcacctagtc actattgtct ttgttcattg tttgatcctg
     2761 agtggttgat tgatatagct ttgaatcttt tctagtccaa gtttgaaaac actgttctgg
     2821 ccctaagggc tggctatgac ctttactgtt gaacctgata gggcagggaa gctttgaaca
     2881 tcaagaaaaa atttttatct taaataaata aatatatata ttcacacacc agtgctttta
     2941 agcaaaaacc agtttttttg tttgtttgtt ttgctttgtg caggttttct ttaagattaa
     3001 aaaaacacaa actatagcag ggtagataat agtttattga ttatattttg ttttagtaaa
     3061 agtatttaat tttaaattct gtgtaattta tctaatttgt atttattcat ttgttatttt
     3121 tatgttgtaa ttaacactta tttacctgga ttaccctaaa aaaattaagc tcttgaaaac
     3181 aaaaaaatga cattaccttg acaggcttct ctttgccaaa agtaatgtat gttctgccaa
     3241 gaagcaaatg agaatgttag actaagtttc tcctgtgtta gtggaaaaca agtgtgtcta
     3301 tctttaaata ttctttgaat caggagtttg cgttgtgtca catcatatgg ctttgcagat
     3361 cttttaaaaa ctgatgttaa ataacttata agtccaactt cttaatatca agataaatga
     3421 atagttcacc aatttgggaa ggagggcggg ttgcaatttg agatagttta aggtttaatt
     3481 attgaaagac tgccatcttg tggcaccaaa aggtaatttt tacatgatta tttgtatagt
     3541 gataagcagt cctgctgtgt tagcctggtc cattatagat atggcatagt tttgggttct
     3601 ctaaatgggt ccatacctct tttgggaaaa aacagggcca cctcataata tttgcctgat
     3661 tatgaatgga attaccttag aaataaggaa ctaatcagat tacttaaccc caatgacaaa
     3721 atccacaaaa attttgaagg cagagaaaca gaaggaatcc agtgatgttt tagctccatt
     3781 agtctaatag gtcagatatt aaaaaattgt tcatatcaaa attaccttat atggattatt
     3841 gccatgtttt ttgagagtta attatttact gttttctaat tcttgccagt atttatgaac
     3901 agctgtagct tgatatttac ctactgaatt ttaggagaac taatggtcac agtttgggtt
     3961 cttttatgtg tatgttttta aaacagctat tttgtgaatc taggtggttg gtttttagaa
     4021 gatttcagga gatgcagtcc agcacaatta gagctggaac attgttacag caggcttttt
     4081 gttgctcatg ggcagataga gggaaagaat cagttgttag ccccaaattt ccacatttca
     4141 gtgttgtaaa ctctgaatgt gataggtaga tgtgggctaa gaataatttc ctccagtgaa
     4201 gacacgggag aagagcttct attatgaggt tatagataag gccttagtta tatcatggaa
     4261 atggactcca tgtagttcta gaactgcatt tcccaaagta tatcctgtga gatgctccac
     4321 agaaaaaatg attgcatgga ctaagtttga aaaatgttgc atgctactct tcccctcgaa
     4381 gatgacagtg gatgtcagca ttaaagactt tgacaggtct ttctgtgaaa aaaagaaagc
     4441 cacataactt tgttgaactt tgcccaaatt aatttttcat tttaaaaaac ttaatagccc
     4501 atgaaaagca aatttgggaa atacattttt taggaaaata ctgtcccaga atgccataat
     4561 tagggtagtt aataattaac acttccattt ctcttactgg ttgttgggag aatctgattg
     4621 aaaattagaa ttactttgtt ttaacttgca gacatgttca tcgtgagtga atgctggatt
     4681 aaatagaatt ttctgtgtct gcaacttgtt tcatgacacc ttggttttct taatcatctt
     4741 gtattattat ttgagtgcaa cattattctt atgcatgggt gactgaaaaa cctgaaacaa
     4801 gattacttgt taaaaaaaag tctaagagac taatgactca tgcttatggc tcagtgggta
     4861 atgaatcagt gtcactgatt cttactttta aaatgtgtag tcagtgaaca ttttctaaat
     4921 acttttcttt gggataccat atgaagatct aattccttat gtttcagtgt aggaacagct
     4981 accttctcaa atagaatatg cagggaggga agtaggcaaa ccatttacaa gttgggtttt
     5041 tgttttgatt aacctgttaa gagacacttt tgtaatctct agtgtttaca ttcctttata
     5101 ctagctatct ataaagagaa tccagaaggc taaattaatc agaaataatt atatttctat
     5161 gtcagggtat tggctatatg tagcatgaac aattctccat tttctgcaaa tgggtttttc
     5221 aggacagttt tataattctg agtttgcttt ttcttattcc ctagacagtt tgtactcagg
     5281 gaatacaaat tgatttttac tgttaaatga gacatagttt agaaaaaatc ataagcaact
     5341 attgtagatt tttgatctgt ttggatttat ctgtagcatt gaataatgtg cagtgtactg
     5401 agttaatgta ggcacctcac tttggattta taaaatgtaa aaacctggaa catgcctgcc
     5461 acagacacca ctttgtctat tcaccgggta ctaatttaca tcctattaat ggttgtaaaa
     5521 gctgagtgtc taaatgctag acatgttcac aattttgtct gtttttgttt atttgttttt
     5581 aagacccaaa ccttttcaat cttgcattta tttacttggt tctcctgtgt caccctgtgg
     5641 ctttttaaat agggaatgta atttatttta atagtagttc tcaaagactc tattaaaaac
     5701 tctggacgag ggatatgtgc aatctgttgg taagtcacat cctatgtttg gcctcatttt
     5761 tataactcct aaaatgagaa aggtagattt taacacttct aaggtacctt tgagctttaa
     5821 agttcttttc tgcttttcca tatgatttgt gttagtgttc actttttata tatttagagt
     5881 ttttatatga gtgtgaatgt ttctgttttg atttggtgag gcatgtcaga ggtatatact
     5941 tgaatatttt tctaaataca gctgctggag gaatgcaaat ggcaaaaatc atccagaaaa
     6001 ccaaacacgg tgaaacatca catccatttg gcatgaagca aagcattaca aatttggagt
     6061 gatgaaagtg ttccatatat tttttatagt gatttttcga tctagagacc cttcatattg
     6121 tttacttact attacgataa ctttgagagg taaccagaga aagcattttt gccatttcca
     6181 gatgaggaag caggaccaga aagctcttca taaagcttta acaccataca agtatccagc
     6241 agtgatcaat tgctctttat aggaaaccac ctttcctctg gggaagaaca gtctgaatta
     6301 ataaaacatc agagtggttg agtagtaaaa ataaacacag atgtattgct ttcaagtctt
     6361 agagtgacat aaagtagtct taaataaata gttcttagtt tattttaagc agttgattgt
     6421 actaacgaga tgtaattatc tgaagtattt atgattagca tattggtttt tataagtatt
     6481 tctgagttat tgagggtgct taaaaatgct gaagaaactt taaaacatga ttcttcttat
     6541 aattttatgt gattcttata gcacaatcat ttttgaaaaa ggaaaactag gctgggtgta
     6601 gtggctcatg cctataatcc cagcactttg ggaggccaaa gtgggtggat cacccgagtt
     6661 caggagttcg agaccagtct ggccaacatg gtgaaacctc gtctctacta aaaataccaa
     6721 aattatccgg tgtggtgaca ggctgcttgt aatcccagct actcgggagg ctgaggcacg
     6781 ggaatctctt gaacctggga ggcagagatt gcaataagcc aagatcatgc cactgcatgc
     6841 cagcctgggt gacagcacaa gactgtctca aaaaaaaaca aaaaaaggaa aaagaaaact
     6901 agccccagtc cctgtctaaa ttgttatagt ttttgactat tatctgaatt agtcttatcc
     6961 aaatttcata tgcagattga tttttcattt tgtatgtata ttacatgata taactatttt
     7021 catagaatat atttctaatt tacaaacact gaattattag cttgatagag tttctcccaa
     7081 accttataaa actttgaaat gtacttatgt ttgagcttta aaaattataa aaacttcatt
     7141 taaacaagtt gctggcatca ttactagcct ttccacctat aattatgatg catttgatgc
     7201 cactgaacag taatagcaca aagaacaggt cacacagtct agactggatt gagttttaat
     7261 taaacttctt catccatagc ctgataagaa tcaggctggg tctagaagag ttatcagact
     7321 gtaggcctgt attaattttg caagctaatg ttaatgttta ctgccagcct tgcatagcaa
     7381 tgccagtttg cagttgtgac tatgatgtga cttttggtaa aaatggttta tatctaaagt
     7441 agtttagatt ttatggaagt ggctcttgat tgtttggaca agcagggtag gtaaatttca
     7501 attacgtttg aagtgtaatt aaatcctaga atagagcact ctccagaaaa aaaaatgaaa
     7561 aaagaatttg gagaatatcc tggcatgaaa acttttttga ataatacgta gaattttgac
     7621 aaggttcata cagtgcatac agtgtgacct tttctgaggt gggcagggag ggttgctacg
     7681 ttgtgttgat ttaaaatttc aaggaggaac aaaaacattc tagccaagga gaattgcatt
     7741 gttatttttt tttctactaa tttaaacgtg catttttcac agttacacat ttaagttttg
     7801 ctaccaaaaa tggccatttt tctagacaga taacattttc ctaaagtgga agagagatcc
     7861 actgttctaa aaaaaaaaaa aaaaaaaaac actaaattgt gccttctccc ttaaattata
     7921 agaagagtaa tagtaaaaac ttgtttaaac tatattttct ttttttctaa tccctctcag
     7981 ttctccttag atctgcctcc tacaaatgat catgttgttt gcatctgcct gattgagttc
     8041 ccttggtcaa acctctccct atcactatca gaagttgttc actaaatgta ttttcctatt
     8101 tctttcatgt tgattgactt tataagtcac cttggtaaac ataacatttg gctaccttct
     8161 catgctttat aaatgaattt gatgaatggc agtttttata ttttaaccct ttaaaaagtt
     8221 tgtcaaggaa acaactcaga gctaagaaca aaatctaact tggaatcagt ttcttccttt
     8281 ggctgctgaa gcagcagcaa gtcacgtaac ctcatttagt tttttgattt acaaaatgtg
     8341 gataataacc tacctcacag atgtgtgtga gcaactgttt gaaagccctt gtttaaatac
     8401 agggtttata tccccacatt caatgtaaat tccttttttt aaaaaaaata agtatctttg
     8461 atactgatag gatgagtatc ttgattttta ggtaagatca cagtttcaca aattgtggtg
     8521 ttgttaggtg acatttaata tttcttgaga cctcaagtat tcaaatatca aacttgtcct
     8581 gtgttgtaac aaatattcag aatctcttaa aatgttggct gaagctgcct taatgtacca
     8641 aaatacagct ctcaagactg ttcacaaata tctgtggttg tacaatgttt tgaacattta
     8701 atgtaaagtt gttgtagaac tgtgaactct aaggatagta tctttattgt tttaggaaga
     8761 atggttgtgt tctttgtcat gtatgcttaa atatacagtg gattttgaat gcaacaaata
     8821 aaactgaaaa cagccatttg gttgtgtcat gacaatcata atataggata taatatttac
     8881 tttttttcaa aaacatcctg cattcattca ttcttagcat acctttgaaa aagaaaaaaa
     8941 aatccatagg ttgatacatt gacccattga agtacttcat tacgtattta ttgaaaattt
     9001 tttttaattg ttgaaattgg gaccactttt tataattggt ttcaagaaaa aaaaatgaac
     9061 agacttccag tgcttttctg ttcaaataaa ctccgattca aatttcagtt ttgtttgtta
     9121 tatacttttt gtgagtgtta aatgatatgg gaaggatttg tctttcatta accatatgga
     9181 aaagatgtct tcctgaatta catagcattt catagttaca gattcttcct aattaatgtt
     9241 tgcaccatat gaaactcata ggtaacaaac acaaaggaga aaagcagcct gtctgattgc
     9301 ttataagctt ttttcacaaa atcaaattaa aagtctctga ttcatttgaa agcta
//
