LOCUS       NM_006251               5254 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens protein kinase AMP-activated catalytic subunit alpha 1
            (PRKAA1), transcript variant 1, mRNA.
ACCESSION   NM_006251
VERSION     NM_006251.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Santana,C.V.N., Magno,L.A.V., Ramos,A.V., Rios,M.A., Sandrim,V.C.,
            De Marco,L.A., de Miranda,D.M. and Romano-Silva,M.A.
  TITLE     Genetic Variations in AMPK, FOXO3A, and POMC Increase the Risk of
            Extreme Obesity
  JOURNAL   J Obes 2024, 3813621 (2024)
   PUBMED   39484290
  REMARK    GeneRIF: Genetic Variations in AMPK, FOXO3A, and POMC Increase the
            Risk of Extreme Obesity.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Jeon,H.Y., Lee,A.J., Moon,C.H. and Ha,K.S.
  TITLE     Regulation of AMPK and GAPDH by Transglutaminase 2 Plays a Pivotal
            Role in Microvascular Leakage in Diabetic Retinas
  JOURNAL   Diabetes 73 (10), 1756-1766 (2024)
   PUBMED   39024127
  REMARK    GeneRIF: Regulation of AMPK and GAPDH by Transglutaminase 2 Plays a
            Pivotal Role in Microvascular Leakage in Diabetic Retinas.
REFERENCE   3  (bases 1 to 5254)
  AUTHORS   Kong,M., Chen,Z., Lin,Z., Yin,P. and Zhao,Q.
  TITLE     SIGMAR1 targets AMPK/ULK1 pathway to inhibit SH-SY5Y cell apoptosis
            by regulating endoplasmic reticulum stress and autophagy
  JOURNAL   Funct Integr Genomics 24 (4), 134 (2024)
   PUBMED   39107544
  REMARK    GeneRIF: SIGMAR1 targets AMPK/ULK1 pathway to inhibit SH-SY5Y cell
            apoptosis by regulating endoplasmic reticulum stress and autophagy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5254)
  AUTHORS   Yu,X., Feng,M., Guo,J., Wang,H., Yu,J., Zhang,A., Wu,J., Han,Y.,
            Sun,Z., Liao,Y. and Zhao,Q.
  TITLE     MLKL promotes hepatocarcinogenesis through inhibition of
            AMPK-mediated autophagy
  JOURNAL   Cell Death Differ 31 (8), 1085-1098 (2024)
   PUBMED   38783090
  REMARK    GeneRIF: MLKL promotes hepatocarcinogenesis through inhibition of
            AMPK-mediated autophagy.
REFERENCE   5  (bases 1 to 5254)
  AUTHORS   Lv,T., Fan,X., He,C., Zhu,S., Xiong,X., Yan,W., Liu,M., Xu,H.,
            Shi,R. and He,Q.
  TITLE     SLC7A11-ROS/alphaKG-AMPK axis regulates liver inflammation through
            mitophagy and impairs liver fibrosis and NASH progression
  JOURNAL   Redox Biol 72, 103159 (2024)
   PUBMED   38642501
  REMARK    GeneRIF: SLC7A11-ROS/alphaKG-AMPK axis regulates liver inflammation
            through mitophagy and impairs liver fibrosis and NASH progression.
REFERENCE   6  (bases 1 to 5254)
  AUTHORS   Stapleton,D., Woollatt,E., Mitchelhill,K.I., Nicholl,J.K.,
            Fernandez,C.S., Michell,B.J., Witters,L.A., Power,D.A.,
            Sutherland,G.R. and Kemp,B.E.
  TITLE     AMP-activated protein kinase isoenzyme family: subunit structure
            and chromosomal location
  JOURNAL   FEBS Lett 409 (3), 452-456 (1997)
   PUBMED   9224708
REFERENCE   7  (bases 1 to 5254)
  AUTHORS   Hawley,S.A., Davison,M., Woods,A., Davies,S.P., Beri,R.K.,
            Carling,D. and Hardie,D.G.
  TITLE     Characterization of the AMP-activated protein kinase kinase from
            rat liver and identification of threonine 172 as the major site at
            which it phosphorylates AMP-activated protein kinase
  JOURNAL   J Biol Chem 271 (44), 27879-27887 (1996)
   PUBMED   8910387
REFERENCE   8  (bases 1 to 5254)
  AUTHORS   Woods,A., Cheung,P.C., Smith,F.C., Davison,M.D., Scott,J.,
            Beri,R.K. and Carling,D.
  TITLE     Characterization of AMP-activated protein kinase beta and gamma
            subunits. Assembly of the heterotrimeric complex in vitro
  JOURNAL   J Biol Chem 271 (17), 10282-10290 (1996)
   PUBMED   8626596
REFERENCE   9  (bases 1 to 5254)
  AUTHORS   Stapleton,D., Mitchelhill,K.I., Gao,G., Widmer,J., Michell,B.J.,
            Teh,T., House,C.M., Fernandez,C.S., Cox,T., Witters,L.A. and
            Kemp,B.E.
  TITLE     Mammalian AMP-activated protein kinase subfamily
  JOURNAL   J Biol Chem 271 (2), 611-614 (1996)
   PUBMED   8557660
REFERENCE   10 (bases 1 to 5254)
  AUTHORS   Munday,M.R., Campbell,D.G., Carling,D. and Hardie,D.G.
  TITLE     Identification by amino acid sequencing of three major regulatory
            phosphorylation sites on rat acetyl-CoA carboxylase
  JOURNAL   Eur J Biochem 175 (2), 331-338 (1988)
   PUBMED   2900138
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008810.8.
            
            On Mar 25, 2021 this sequence version replaced NM_006251.5.
            
            Summary: The protein encoded by this gene belongs to the ser/thr
            protein kinase family. It is the catalytic subunit of the
            5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular
            energy sensor conserved in all eukaryotic cells. The kinase
            activity of AMPK is activated by the stimuli that increase the
            cellular AMP/ATP ratio. AMPK regulates the activities of a number
            of key metabolic enzymes through phosphorylation. It protects cells
            from stresses that cause ATP depletion by switching off
            ATP-consuming biosynthetic pathways. Alternatively spliced
            transcript variants encoding distinct isoforms have been observed.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037303.2, SRR18074967.3355938.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397128.7/ ENSP00000380317.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               AC008810.8         45392-45703
            313-454             AC008810.8         66180-66321
            455-548             AC008810.8         68263-68356
            549-693             AC008810.8         71903-72047
            694-781             AC008810.8         74263-74350
            782-1006            AC008810.8         76076-76300
            1007-1493           AC008810.8         78528-79014
            1494-1620           AC008810.8         79126-79252
            1621-5254           AC008810.8         80744-84377
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.1"
     gene            1..5254
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /note="protein kinase AMP-activated catalytic subunit
                     alpha 1"
                     /db_xref="GeneID:5562"
                     /db_xref="HGNC:HGNC:9376"
                     /db_xref="MIM:602739"
     exon            1..312
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            313..454
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            455..548
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            549..693
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            694..781
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..1006
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1493
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1620
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..5254
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4797..4802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /note="hexamer: AATAAA"
     polyA_site      4821
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /note="major polyA site"
     regulatory      5234..5239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
                     /note="hexamer: AATAAA"
     polyA_site      5254
                     /gene="PRKAA1"
                     /gene_synonym="AMPK; AMPK alpha 1; AMPKa1"
ORIGIN      
        1 attctcctgg cagcccccgc ccgccccgcc agggggagcg tctgcgcccg acgtaggctg
       61 cggggcctgg gctggcggcg cagggcggtg actcggctcg gtggctgccc tcccgcgtcc
      121 ccaccccctc cccgcccgcc cccggcgcgg ccctgcccgc cccctccgcc ccctcccgca
      181 gcgccatgcg cagactcagt tcctggagaa agatggcgac agccgagaag cagaaacacg
      241 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
      301 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
      361 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
      421 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
      481 atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga
      541 atggaaggct ggatgaaaaa gaaagtcggc gtctgttcca acagatcctt tctggtgtgg
      601 attattgtca caggcatatg gtggtccata gagatttgaa acctgaaaat gtcctgcttg
      661 atgcacacat gaatgcaaag atagctgatt ttggtctttc aaacatgatg tcagatggtg
      721 aatttttaag aacaagttgt ggctcaccca actatgctgc accagaagta atttcaggaa
      781 gattgtatgc aggcccagag gtagatatat ggagcagtgg ggttattctc tatgctttat
      841 tatgtggaac ccttccattt gatgatgacc atgtgccaac tctttttaag aagatatgtg
      901 atgggatctt ctatacccct caatatttaa atccttctgt gattagcctt ttgaaacata
      961 tgctgcaggt ggatcccatg aagagggcca caatcaaaga tatcagggaa catgaatggt
     1021 ttaaacagga ccttccaaaa tatctctttc ctgaggatcc atcatatagt tcaaccatga
     1081 ttgatgatga agccttaaaa gaagtatgtg aaaagtttga gtgctcagaa gaggaagttc
     1141 tcagctgtct ttacaacaga aatcaccagg atcctttggc agttgcctac catctcataa
     1201 tagataacag gagaataatg aatgaagcca aagatttcta tttggcgaca agcccacctg
     1261 attcttttct tgatgatcat cacctgactc ggccccatcc tgaaagagta ccattcttgg
     1321 ttgctgaaac accaagggca cgccataccc ttgatgaatt aaatccacag aaatccaaac
     1381 accaaggtgt aaggaaagca aaatggcatt taggaattag aagtcaaagt cgaccaaatg
     1441 atattatggc agaagtatgt agagcaatca aacaattgga ttatgaatgg aaggttgtaa
     1501 acccatatta tttgcgtgta cgaaggaaga atcctgtgac aagcacttac tccaaaatga
     1561 gtctacagtt ataccaagtg gatagtagaa cttatctact ggatttccgt agtattgatg
     1621 atgaaattac agaagccaaa tcagggactg ctactccaca gagatcggga tcagttagca
     1681 actatcgatc ttgccaaagg agtgattcag atgctgaggc tcaaggaaaa tcctcagaag
     1741 tttctcttac ctcatctgtg acctcacttg actcttctcc tgttgaccta actccaagac
     1801 ctggaagtca cacaatagaa ttttttgaga tgtgtgcaaa tctaattaaa attcttgcac
     1861 aataaacaga aaactttgct tatttctttt gcagcaataa gcatgcataa taagtcacag
     1921 ccaaatgctt ccatttgtaa tcaagttata cataattata accgagggct ggcgttttgg
     1981 aatgcaattt gcacagggat tggaacatga tttatagtta aaagcctaat atgcagaaat
     2041 gaattaagat cattttgttg ttcattgtgc agtatgtata tagcataata tacacagtga
     2101 attataggtc tcaggcttac ttgatttttg gctattttat atttagtgta cacagggctt
     2161 tgaaatatta atttacataa aggccttcat atattattac gtgttatata ttacgtgtta
     2221 taaatttatt caataaatat ttgcctagaa ttcccaagac ctttataggt gattttgttt
     2281 tctgggctcc ttaacttcat aaatagctag tatcttccag cagtagtaac agtctggata
     2341 acttcttcca tatccctccc tctttgtttt tttgagacag tgtcactttg tcacccaggc
     2401 tggagtgcaa tggtgtggtc tcggctcact gcaacctcca cctcccgggt tcaagtgatt
     2461 ctcccgcctc agcttcctga gtagctggaa ctacaggcgt gtgccaccac acccggctaa
     2521 tttttcgtat ttttagtgta gacggggttt cactatgttg cccaggctgg tctcgaactc
     2581 ctgaccgcgt gatccaccac ctcagcttcc caaagtggtg ggattacagg cgtgagccac
     2641 cgcacccggc ctccatatcc cccttttaaa attctgtagt gtatggtaag tcatatcaga
     2701 tatcagacct aatttaaatt tcattttagc tttacaagtc caaaaacaca gaatttatat
     2761 attcagatac tctagcacta attttagtct taaaatattc ccacgatatt ctgtacacaa
     2821 aatgttcttt ttgttacaag agctgagttg catatactgt agataaatca tattattttt
     2881 gccaatttca caaattcctc tggcccatca tgtcagtcat tattgagtat atgcacacat
     2941 tgctacttat ttgattatgt atcttttaaa ttgattcagt gcatagaaaa ctatctctta
     3001 caaactttaa gtgctctgat atgacttccc ccccaaattt tattatgaac atttttaaaa
     3061 acagaaaaat tgaaaaactg tttggtaagc acatgtatat ctaccattta gattcagcag
     3121 ttgttaatgt tttgtcattt gttttctcta tacctatata tgtatagata cagctagtta
     3181 tgcatatata tgcatatatg tgtttgtttg tgtatgtata tatgcttttt tccccctgaa
     3241 ccatttggat gttacagaca tacttatcac cgtgaaaata cttcaagtat ctcctacaga
     3301 taatgacatt ctcctaaaaa tccgtaatac cattgtaaaa gtaataattc cccaatatca
     3361 tctaatcaag ccatatttaa atttctgaag ttaactccaa atttctttat agctgattat
     3421 ttcaaactag gatccaatta aagtttacat atgacacttg gttataactc tttagttgga
     3481 tataacatta ttattatttt gataaaatat ggaacaaatc aattctatta ataagtggtc
     3541 acatttgttt tgggcttaaa ttacttttta aagatactgg attttcctaa gatttctgat
     3601 ttacactgat attttttttt gtcattctta attgcatcac acaatagatg taaatgaaga
     3661 tgtagtcacc tcagataaaa ttggtatcgt gtatgataat attgtatcat ttatatttgc
     3721 cttatgttaa ctttaagaaa ttgatttttt tgtattaatc attttcccat tgcaacagag
     3781 ctatattttt tctattttaa gaatcatatt ttaggattat ttttggcaaa tacagtgagc
     3841 acttatgtaa ccagatgata atgaactcaa atgtcatgat agcttgcata aatggtgact
     3901 ctagtagatt tgactcaagc acttctagaa tcatgcactg aattcaaaag aaaaatcttg
     3961 ctgctttttg tccagggctt gttctattca acttctaatt tgaaagctgt acaaagtaat
     4021 agaagttcca tttaaatatg agttcaaaac tgtatttact ttttatgtgg ccctctcttt
     4081 aggggattct aattttactt agggtctcta agtgcagcat aatgttcctg atgttaacag
     4141 aagactgtat ttttaaagtt acaaatttgt atatggaatt aagtaatggc gctatatacg
     4201 ctgttgtggg gaggggggaa gaaaaggagg aaccaattaa ataggacctt ttaaaaattg
     4261 ttaattttgt aaactttgct tctcttataa gttattgtga ttcattttag ttactgtgtt
     4321 ttattttgaa aatatttaaa tattgcactt ctataaatag tatgataaat gcacagacaa
     4381 ttgcagtaaa ttctttttta agctaggata tttgaaatga caacctttgg ttaagtgtgt
     4441 caaggttgca acagaatttt cacaattttt ttgttgtttg caaattgtta ctaatattga
     4501 agaggtaagg gaggcaatgc aaatgatttt taatcttttt ttattatctt ttcagcagtt
     4561 tatatttttt gtgactttat gcaaccatat ttttactttg tcttgacaac tgaaagatgt
     4621 ataaggtttt ttgccagaaa tgtactgtat acatagtttt aagtataaca gattttactg
     4681 atatgtaaaa attttgccat taaaataaat gatttctcac tgagaggaac ttttctacca
     4741 ggttggggca tatgggagct taatatatca tatctaattt aaaataattt cactgaaata
     4801 aactccattg cttttaccta atttttttct tgagatgctt ttgtagtttt tcagagtttt
     4861 agatgatttt atacaaaatc ctctgcctag cactgctctt tttgatgttg tagtgacacc
     4921 atttacattg aattaatgct tggtagcctg gggctagatg tggaactcca tggatctgtg
     4981 ttctgactgg cacctttgga atgaaagaaa agtgtgtgct gtccaaattt tttcccctta
     5041 attctttccc tcatcttctc acccataata gaaattttat ttccattgtg agttctgaca
     5101 agaatgaaat tccacataca acataactgt aaattgttgg taggtagaag ttaatatttg
     5161 tggttcatgt atattttgac cagagtatat ttaagtatat aatttcagct tccttgattt
     5221 agaaatatga tataataaag aaaaactcca ttta
//
