LOCUS       NM_000947               2303 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens DNA primase subunit 2 (PRIM2), transcript variant 1,
            mRNA.
ACCESSION   NM_000947 XM_942683 XM_946221
VERSION     NM_000947.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Sun,R., Shao,X., Akter,F., Zahid,K.R., Yao,S., Ma,L. and Xu,G.
  TITLE     PRIM2: A Marker of MYC-driven Hyper-proliferation, Disease
            Progression, Tumor Aggressiveness and Poor Survival in Glioma
            Patients
  JOURNAL   Cancer Genomics Proteomics 21 (2), 186-202 (2024)
   PUBMED   38423596
  REMARK    GeneRIF: PRIM2: A Marker of MYC-driven Hyper-proliferation, Disease
            Progression, Tumor Aggressiveness and Poor Survival in Glioma
            Patients.
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Mu,R., Liu,H., Luo,S., Patz,E.F. Jr., Glass,C., Su,L., Du,M.,
            Christiani,D.C., Jin,L. and Wei,Q.
  TITLE     Genetic variants of CHEK1, PRIM2 and CDK6 in the mitotic
            phase-related pathway are associated with nonsmall cell lung cancer
            survival
  JOURNAL   Int J Cancer 149 (6), 1302-1312 (2021)
   PUBMED   34058013
  REMARK    GeneRIF: Genetic variants of CHEK1, PRIM2 and CDK6 in the mitotic
            phase-related pathway are associated with nonsmall cell lung cancer
            survival.
REFERENCE   3  (bases 1 to 2303)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2303)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2303)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2303)
  AUTHORS   Schneider,A., Smith,R.W., Kautz,A.R., Weisshart,K., Grosse,F. and
            Nasheuer,H.P.
  TITLE     Primase activity of human DNA polymerase alpha-primase. Divalent
            cations stabilize the enzyme activity of the p48 subunit
  JOURNAL   J Biol Chem 273 (34), 21608-21615 (1998)
   PUBMED   9705292
REFERENCE   7  (bases 1 to 2303)
  AUTHORS   Copeland,W.C.
  TITLE     Expression, purification, and characterization of the two human
            primase subunits and truncated complexes from Escherichia coli
  JOURNAL   Protein Expr Purif 9 (1), 1-9 (1997)
   PUBMED   9116489
REFERENCE   8  (bases 1 to 2303)
  AUTHORS   Shiratori,A., Okumura,K., Nogami,M., Taguchi,H., Onozaki,T.,
            Inoue,T., Ando,T., Shibata,T., Izumi,M., Miyazawa,H. et al.
  TITLE     Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B)
            subunit genes of the human DNA primase to chromosome bands 1q44 and
            6p11.1-p12
  JOURNAL   Genomics 28 (2), 350-353 (1995)
   PUBMED   8530050
REFERENCE   9  (bases 1 to 2303)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur J Biochem 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   10 (bases 1 to 2303)
  AUTHORS   Wang,T.S., Hu,S.Z. and Korn,D.
  TITLE     DNA primase from KB cells. Characterization of a primase activity
            tightly associated with immunoaffinity-purified DNA
            polymerase-alpha
  JOURNAL   J Biol Chem 259 (3), 1854-1865 (1984)
   PUBMED   6693436
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC017833.1 and BC064931.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000947.4.
            
            Summary: This gene encodes the 58 kilodalton subunit of DNA
            primase, an enzyme that plays a key role in the replication of DNA.
            The encoded protein forms a heterodimer with a 49 kilodalton
            subunit. This heterodimer functions as a DNA-directed RNA
            polymerase to synthesize small RNA primers that are used to create
            Okazaki fragments on the lagging strand of the DNA. Alternative
            splicing of this gene results in multiple transcript variants. This
            gene has a related pseudogene, which is also present on chromosome
            6. [provided by RefSeq, Apr 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064931.1, X74331.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000615550.5/ ENSP00000484105.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BC017833.1         14-15
            3-1977              BC064931.1         1-1975
            1978-2303           BC064931.1         1977-2302
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p11.2"
     gene            1..2303
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /note="DNA primase subunit 2"
                     /db_xref="GeneID:5558"
                     /db_xref="HGNC:HGNC:9370"
                     /db_xref="MIM:176636"
     exon            1..72
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            73..235
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1611
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /EC_number="2.7.7.-"
                     /note="isoform a is encoded by transcript variant 1; DNA
                     primase large subunit; DNA primase 58 kDa subunit;
                     primase, DNA, polypeptide 2 (58kDa); primase (DNA) subunit
                     2"
                     /codon_start=1
                     /product="DNA primase large subunit isoform a"
                     /protein_id="NP_000938.2"
                     /db_xref="CCDS:CCDS75476.1"
                     /db_xref="GeneID:5558"
                     /db_xref="HGNC:HGNC:9370"
                     /db_xref="MIM:176636"
                     /translation="MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFEN
                     LAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHIS
                     HFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAISDEEKT
                     LREQEIVASSPSLSGLKLGFESIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAII
                     LNEFRAKLSKALALTARSLPAVQSDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQI
                     DLLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKG
                     KMDPDKFDKGYSYNIRHSFGKEGKRTDYTPFSCLKIILSNPPSQGDYHGCPFRHSDPE
                     LLKQKLQSYKISPGGISQILDLVKGTHYQVACQKYFEMIHNVDDCGFSLNHPNQFFCE
                     SQRILNGGKDIKKEPIQPETPQPKPSVQKTKDASSALASLNSSLEMDMEGLEDYFSED
                     S"
     exon            236..339
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            340..419
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            420..540
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            541..636
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            637..774
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            775..842
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            843..915
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1101
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1228
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1311
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1380
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..2303
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2286..2291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /note="hexamer: AATAAA"
     polyA_site      2303
                     /gene="PRIM2"
                     /gene_synonym="p58; PRIM2A"
                     /note="major polyA site"
ORIGIN      
        1 atatgaactc tcccgccacc cgggaacagt ggctgccacc gtttgtgttt tcccgagttt
       61 gaattcttgc aggtgaccaa gatggagttt tctggaagaa agtggaggaa gctgaggttg
      121 gcaggtgacc agaggaatgc ttcctaccct cattgccttc agttttactt gcagccacct
      181 tctgaaaaca tatctttaat agaatttgaa aacttggcta ttgatagagt taaattgtta
      241 aaatcagttg aaaatcttgg agtgagctat gtgaaaggaa ctgaacaata ccagagtaag
      301 ttggagagtg agcttcggaa gctcaagttt tcctacagag aaaacttaga agatgaatat
      361 gaaccacgaa gaagagatca tatttctcat tttattttgc ggcttgctta ttgccagtct
      421 gaagaactta gacgctggtt cattcaacaa gaaatggatc tccttcgatt tagatttagt
      481 attttaccca aggataaaat tcaggatttc ttaaaggata gccaattgca gtttgaggct
      541 ataagtgatg aagagaagac tcttcgagaa caggagattg ttgcctcatc accaagttta
      601 agtggactta agttggggtt cgagtccatt tataagatcc cttttgctga tgctctggat
      661 ttgtttcgag gaaggaaagt ctatttggaa gatggctttg cttacgtacc acttaaggac
      721 attgtggcaa tcatcctgaa tgaatttaga gccaaactgt ccaaggcttt ggcattaaca
      781 gccaggtcct tgcctgctgt gcagtctgat gaaagacttc agcctctgct caatcacctc
      841 agtcattcct acactggcca agattacagt acccagggaa atgttgggaa gatttcttta
      901 gatcagattg atttgctttc taccaaatcc ttcccacctt gcatgcgtca gttacataaa
      961 gccttgcggg aaaatcacca tcttcgtcat ggaggccgaa tgcagtatgg cctatttctg
     1021 aagggcattg gtttaacttt ggaacaggca ttgcagttct ggaagcaaga atttatcaaa
     1081 ggaaagatgg atccagacaa gtttgataaa ggttactctt acaacatccg tcacagcttt
     1141 ggaaaggaag gcaagaggac agactataca cctttcagtt gcctgaagat tattctgtcc
     1201 aatccaccaa gccaagggga ttatcatggg tgcccattcc gtcacagtga tccagagctg
     1261 ctgaagcaaa agttgcagtc atacaagatc tctcctggag ggataagcca gattttggat
     1321 ttagtaaagg ggacacatta ccaggtagcc tgtcaaaaat actttgagat gatacacaat
     1381 gtggatgatt gtggcttttc tttgaatcat cctaatcagt tcttttgtga gagccaacgt
     1441 attctaaatg gtggtaaaga cataaagaag gaacctatcc aaccagaaac tcctcaaccc
     1501 aaaccaagtg tccagaaaac caaggatgca tcatctgctc tggcctcttt aaattcctct
     1561 ctggaaatgg atatggaagg actagaagat tactttagtg aagattctta ggcagtttta
     1621 taaccctttt tcctcaatag cctgtttcct gtttttaaga ttttgccttt gttgttgaaa
     1681 aagggtttca ctctgtcacc aaggcttagt gcagtgacac aattacagct gattgcagcc
     1741 ttgaccttcc cagctcaagt gatcctccta cctcagcctc ccaagtagtt aggaccacag
     1801 gtgtgcacct catatccaga taattttttt caattttttt ttgtagaggt ggggggtctc
     1861 cctatgttgc ccaggcagat ctcagactcc tgggctcaag cgatcctcac acctcagcgt
     1921 cccagagtgc tgggattacg gttgtgagcc actgtgcctg gccttttttt tttttttaac
     1981 ctttttgttt aacttctctc ttcactgcat cccaatccat ctacaggcat gcacacttat
     2041 taggaaagga ggtttgaggt aacaacagag actttcacta tattttgctt tgacagaagg
     2101 aaagaggagg agtttctatt aaaatctgtc acttgagtga tgtcatttaa gtcctatttt
     2161 aggagataaa aacagctttg gggactggtt aaagtccccc agaaactaca ataaagaaca
     2221 acttttgttt taactcttaa tcactttgta attttgactc aatccttttc tggaccattt
     2281 ttgttaataa atatcaaagt gta
//
