LOCUS       NM_153026               5878 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1),
            transcript variant 1, mRNA.
ACCESSION   NM_153026 XM_001714454 XM_001718043
VERSION     NM_153026.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Jiang,D., He,Y., Mo,Q., Liu,E., Li,X., Huang,L., Zhang,Q., Chen,F.,
            Li,Y. and Shao,H.
  TITLE     PRICKLE1, a Wnt/PCP signaling component, is overexpressed and
            associated with inferior prognosis in acute myeloid leukemia
  JOURNAL   J Transl Med 19 (1), 211 (2021)
   PUBMED   34001134
  REMARK    GeneRIF: PRICKLE1, a Wnt/PCP signaling component, is overexpressed
            and associated with inferior prognosis in acute myeloid leukemia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5878)
  AUTHORS   Daulat,A.M., Finetti,P., Revinski,D., Silveira Wagner,M.,
            Camoin,L., Audebert,S., Birnbaum,D., Kodjabachian,L., Borg,J.P. and
            Bertucci,F.
  TITLE     ECT2 associated to PRICKLE1 are poor-prognosis markers in
            triple-negative breast cancer
  JOURNAL   Br J Cancer 120 (9), 931-940 (2019)
   PUBMED   30971775
  REMARK    GeneRIF: Authors used a proteomic approach to identify protein
            complexes associated with PRICKLE1. The mRNA expression levels of
            the corresponding genes were assessed in 8982 patients with
            invasive primary breast cancer. AUthors then characterised the
            molecular interaction between PRICKLE1 and the guanine nucleotide
            exchange factor ECT2.
REFERENCE   4  (bases 1 to 5878)
  AUTHORS   Hata,Y., Yoshida,K. and Nishida,N.
  TITLE     Sudden unexpected death with rare compound heterozygous variants in
            PRICKLE1
  JOURNAL   Neurogenetics 20 (1), 39-43 (2019)
   PUBMED   30564977
  REMARK    GeneRIF: showed that the patient's father (p.Asp760del) and mother
            (p.Asp201Asn) each had a mutation in prickle1
REFERENCE   5  (bases 1 to 5878)
  AUTHORS   Kim,S.M., Yang,J.W., Park,M.J., Lee,J.K., Kim,S.U., Lee,Y.S. and
            Lee,M.A.
  TITLE     Regulation of human tyrosine hydroxylase gene by neuron-restrictive
            silencer factor
  JOURNAL   Biochem Biophys Res Commun 346 (2), 426-435 (2006)
   PUBMED   16764822
  REMARK    GeneRIF: NRSF/REST functions as a repressor of TH transcription in
            NSCs via a mechanism dependent on the TH NRSE/RE1 sites.
REFERENCE   6  (bases 1 to 5878)
  AUTHORS   Shimojo,M.
  TITLE     Characterization of the nuclear targeting signal of REST/NRSF
  JOURNAL   Neurosci Lett 398 (3), 161-166 (2006)
   PUBMED   16442230
REFERENCE   7  (bases 1 to 5878)
  AUTHORS   Shimojo,M. and Hersh,L.B.
  TITLE     Characterization of the REST/NRSF-interacting LIM domain protein
            (RILP): localization and interaction with REST/NRSF
  JOURNAL   J Neurochem 96 (4), 1130-1138 (2006)
   PUBMED   16417580
REFERENCE   8  (bases 1 to 5878)
  AUTHORS   Shimojo,M. and Hersh,L.B.
  TITLE     REST/NRSF-interacting LIM domain protein, a putative nuclear
            translocation receptor
  JOURNAL   Mol Cell Biol 23 (24), 9025-9031 (2003)
   PUBMED   14645515
  REMARK    GeneRIF: appears to serve as a nuclear receptor for REST/NRSF,
            REST4, and possibly other transcription factors
REFERENCE   9  (bases 1 to 5878)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of human PRICKLE1 and PRICKLE2
            genes as well as mouse Prickle1 and Prickle2 genes homologous to
            Drosophila tissue polarity gene prickle
  JOURNAL   Int J Mol Med 11 (2), 249-256 (2003)
   PUBMED   12525887
  REMARK    GeneRIF: PRICKLE1 and PRICKLE2 mRNAs were expressed together in
            brain, eye and testis.
REFERENCE   10 (bases 1 to 5878)
  AUTHORS   Mastrangelo,M., Caputi,C., Esposito,D. and Leuzzi,V.
  TITLE     PRICKLE1-Related Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301774
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA413584.1, BC114939.1,
            AC079601.25 and BE858932.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_153026.2.
            
            Summary: This gene encodes a nuclear receptor that may be a
            negative regulator of the Wnt/beta-catenin signaling pathway. The
            encoded protein localizes to the nuclear membrane and has been
            implicated in the nuclear trafficking of the transcription
            repressors REST/NRSF and REST4. Mutations in this gene have been
            linked to progressive myoclonus epilepsy. Alternate splicing
            results in multiple transcript variants. A pseudogene of this gene
            is found on chromosome 3. [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-4 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.15559.1,
                                           SRR1660809.180740.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000345127.9/ ENSP00000345064.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               DA413584.1         25-159
            136-2916            BC114939.1         1-2781
            2917-4220           AC079601.25        26833-28136
            4221-4297           BE858932.1         1-77                c
            4298-5878           AC079601.25        28214-29794
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q12"
     gene            1..5878
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="prickle planar cell polarity protein 1"
                     /db_xref="GeneID:144165"
                     /db_xref="HGNC:HGNC:17019"
                     /db_xref="MIM:608500"
     exon            1..282
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            283..462
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     CDS             331..2826
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="REST (RE-1 silencing transcription factor)/NRSF
                     (neuron-restrictive silencer factor)-interacting LIM
                     domain protein; REST/NRSF-interacting LIM domain protein
                     1; prickle-like 1; prickle homolog 1; REST/NRSF
                     interacting LIM domain protein"
                     /codon_start=1
                     /product="prickle-like protein 1"
                     /protein_id="NP_694571.2"
                     /db_xref="CCDS:CCDS8742.1"
                     /db_xref="GeneID:144165"
                     /db_xref="HGNC:HGNC:17019"
                     /db_xref="MIM:608500"
                     /translation="MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQ
                     IQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSA
                     QRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAVFASRAGPGVCWHPSCFVCFT
                     CNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCC
                     LECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATE
                     ACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSV
                     RMGKSSRSADQCRQSLLLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEF
                     WKGRVEQETPEDPEEWADHEDYMTQLLLKFGDKSLFQPQPNEMDIRASEHWISDNMVK
                     SKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELELDHGASG
                     YNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSLYSLQNF
                     EEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKS
                     QSRPQQVKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSR
                     SDNALNLVTERKYSPKDRLRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSD
                     YGLQNPGMNRFLGLYGEDDDSWCSSSSSSSDSEEEGYFLGQPIPQPRPQRFAYYTDDL
                     SSPPSALPTPQFGQRTTKSKKKKGHKGKNCIIS"
     exon            463..576
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            577..714
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            715..918
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1105
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1969
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..5878
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3814..3819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="hexamer: AATATA"
     polyA_site      3834
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="major polyA site"
     regulatory      4267..4272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="hexamer: AATAAA"
     regulatory      4274..4279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="hexamer: AACAAA"
     polyA_site      4280
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="major polyA site"
     polyA_site      4299
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
     regulatory      5858..5863
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
                     /note="hexamer: AATAAA"
     polyA_site      5878
                     /gene="PRICKLE1"
                     /gene_synonym="EPM1B; RILP"
ORIGIN      
        1 gcactccgcc tgcagcccag ctgccagcgc cgtctcccgc ggccgccgcc gcccgacagc
       61 gcgcccggaa catgccagcc tcgcgcagcc tgcgctgact tccccgagac gcgccgcggt
      121 cggcacggag actttctggg ctctggatgg ttcgtgcgct gctcaccctc gcccacccct
      181 gacgccgcgg accgcagctc ctcagccccg cagccccgca gcccagcagc cccgcagcgg
      241 cgccgaggac agtgcagccg agttgcgtct ccctcgcgcg agcagccatt gtttgatgtc
      301 ctgtgactga gaaaccaggc tttaaacttt atgcctttgg agatggagcc caagatgagc
      361 aaactggcct ttggctgtca gagaagttcc acatcagatg atgactctgg ctgtgcattg
      421 gaggagtacg cctgggtccc cccgggcctg agaccagagc agatccagct ctattttgct
      481 tgcttaccag aggaaaaagt tccttacgtt aacagccccg gagagaagca tcggattaaa
      541 cagcttttgt accagttacc accacatgat aatgaggtac ggtattgcca gtctttgagt
      601 gaagaggaga aaaaagagtt gcaggtgttc agtgctcagc ggaagaaaga agcactggga
      661 agaggaacaa ttaagcttct gtccagagca gtcatgcatg ctgtgtgtga gcagtgtggt
      721 ttgaagataa atggaggtga agttgcagtg ttcgcctccc gtgcgggccc tggtgtgtgc
      781 tggcacccat cctgttttgt ctgtttcacg tgtaatgagc tgctggtcga cctcatctat
      841 ttttatcagg atggaaaaat tcactgtggc aggcaccatg cagaactgct caaaccacgg
      901 tgctcagcat gtgacgagat aatttttgct gatgagtgca cagaagctga gggtcgccat
      961 tggcacatga aacacttctg ctgccttgag tgtgaaacgg tcctgggagg acagaggtat
     1021 atcatgaagg acggccgccc cttctgctgt ggctgttttg agtctctcta tgcggagtac
     1081 tgtgaaacct gtggggaaca tattggtgtg gaccatgcac agatgaccta tgacgggcag
     1141 cactggcacg ccacggaagc ctgcttttct tgtgcccagt gtaaagcctc tttgttggga
     1201 tgtcccttcc ttcccaaaca gggtcagatt tactgctcaa aaacgtgcag tcttggtgaa
     1261 gacgtccatg cctctgattc ttccgactct gcatttcagt cagctcgatc aagagactcc
     1321 cgaagaagtg tccgaatggg caagagcagc cggtcagcag atcagtgtag acagtctctc
     1381 ctcttatcgc ctgctctgaa ctacaagttt cctggcctct caggcaatgc tgatgacacc
     1441 ctttctcgaa aattggatga tctgagtctc tccagacaag gaacaagttt tgccagtgaa
     1501 gaattttgga aaggcagagt agagcaggaa actccagaag accctgaaga atgggctgat
     1561 catgaagatt atatgacgca gctcctcctc aagtttggtg ataaaagcct ctttcagcca
     1621 cagcccaatg agatggatat tcgagccagt gagcactgga tatctgataa catggttaaa
     1681 agtaagaccg agttaaagca aaataaccag agccttgcaa gtaaaaaata ccagtctgat
     1741 atgtactggg cacagtcaca agatggactg ggcgattctg cttatggcag ccacccaggc
     1801 cctgcaagca gtagaaggct tcaggaattg gaactggacc atggggcttc agggtataat
     1861 catgatgaaa cacagtggta tgaagattcc ctggagtgtc tgtcagacct gaaaccagag
     1921 caaagtgttc gggattcgat ggattctttg gcattgtcca atatcacagg ggcttcggtg
     1981 gatggagaaa acaagccaag gccatcattg tattctctgc aaaattttga ggagatggaa
     2041 acagaagatt gtgagaagat gagcaatatg ggaactttga actcttccat gctgcacagg
     2101 agtgcagagt ccttaaagag tctaagttca gagttgtgtc cagagaaaat cctgcctgaa
     2161 gagaagccag tacatctgcc agtgctcaga aggtccaagt ctcaatccag accccagcag
     2221 gtcaagtttt ctgatgatgt cattgacaat gggaactatg acattgaaat ccggcagcct
     2281 ccgatgagtg aaaggactcg gagacgcgtc tacaattttg aagagagggg atccaggtct
     2341 catcaccacc gccgccggag aagtagaaag tcccgctccg acaatgccct gaatcttgtt
     2401 acagaaagaa aatactctcc caaggacaga ctgcggctgt acacccccga taactatgag
     2461 aaatttatac agaataaaag tgcccgggag atccaagcat acatccagaa tgctgatctc
     2521 tacggacagt acgcccatgc cacttccgat tatggcctgc agaacccagg aatgaatcgg
     2581 tttctgggac tctacggcga ggatgatgat tcctggtgtt cttcctcctc ctcctcttcc
     2641 gactcggaag aagaaggata ttttcttgga caaccaatcc ctcaaccccg gccacagaga
     2701 tttgcctact atacagatga cctttctagt ccaccatctg cacttcccac ccctcagttt
     2761 ggtcagagga caacaaaatc caagaagaaa aagggacaca agggcaaaaa ttgtattatt
     2821 tcttaaccaa gtagtgatgc agagcatttg tttaaaactt agccattaac cgtctgaatc
     2881 gtttcctttt cttccgtagg aaagttgtga aaatagttta aagtgctttc tttgcccatg
     2941 taaatgagaa ctcaactgct gtttacagtg tcagattaac atttgaaagg tgtgatttct
     3001 ccaatttacc ctctttggat ggtgccaggt ggacgtgaca tctcgtgcct gtccggtgcg
     3061 ggtgcgttac agatggacgt agctgctttg gttttccagt cctcaaggga atactgaaga
     3121 tgctgactga aggggattgg atgttgattt tagaagatgg agaactccag ccacctttgt
     3181 aaagcactag tgtttgtcat ttatgtaagt caggtcggct caggtcttga tagtccgtct
     3241 tggtgtgagg catgcctgtc acgatgacct agctaacact gtgcatctta ttgtgaggcc
     3301 agcttgtccc ctcgaaccct ctttggccag gtaaacattg tattgtatca gcgccagcta
     3361 cagattgaaa aaaaaaaaca aaaaacattg ctatttataa cttagtattt catagactta
     3421 agtgtattcc taatttaacg gtgcaaatat taatgtatat actgtacagt tcagatttta
     3481 aagctgatat ttttatatcc ctgaattgta agccgtttgt tacgctgcag tgctagattt
     3541 gccagggaac cagaatttat ggatgaactg attgcttata ttttagtcag ggtttataaa
     3601 tgtagatggt caaatttaca ttgcctagtg atggaaaatt caactttttt tgattttttt
     3661 ttccaatatt aaaaaaggct ctgtatgcat ggtggggcta tgtaagtact ctttaaaact
     3721 atggccctat taatcttaca agtgttactt atgggtcaag caatgtaaac tgtataaatg
     3781 taaaaacaac ccctccacac acataacccc tggaatatat ggtaaaaaca agtagaagct
     3841 gtttcagcga gtgggttttt tccccctgcc tgcggggggt gcctttctca ttttcagcaa
     3901 ggtgatgatt atctcatggg aaatgacaaa gatctgcttt tgtggcagag gactttgtag
     3961 atttgttatt ttaagagagg ttattccttt ttattccttc cctcccacta atttgttggc
     4021 ctttaacagc aattttgaaa actgggtctt ctggttatgt ttttgtttta aaatctttaa
     4081 attagaggat gctgtgccat tgagtactgt aagttaatat gaggttctgg ttcaaggaaa
     4141 acttacgttg gatctgaacc aatgagcaga tattttgata tgtgccactc ttgcatatac
     4201 atctcagtcc taactaaagg ttctagtggc atccaggacc tttagggagg catttaataa
     4261 acacttaata aataacaaaa ctacctttct gaatataaat ctttgcaaat gtatccagat
     4321 aacttccatt ttgtgcctgt gttcaggcat gatttatttt gcagttgccc taccattaac
     4381 agacctccat ttgtctcgtg tgtccacacg ccccacttgt aggccagaaa tagggcttca
     4441 gacacagagg cttatttttt ccatatagac tggagtgggg aaaatttggc cttagggagg
     4501 acagacacaa gtccaatggg taaaccagcg agtagtaggt ggacagccgt cccacacaag
     4561 ggtttgtatc tgggctacac agattccctt cagaaaagca ccaatggtga gagagttctt
     4621 cactcagtaa cttacattcc ctggtctggt gcctttaaaa aaccatcagc aatgaaaagg
     4681 aagtgcatac acatgacaaa aaaattcaaa tagggcaaaa gtcagagaca gcatctcctt
     4741 tccattcttc atcccagtcc cccaggtcct ctccccagaa gtaaccactc ttacccagtt
     4801 ttagtgtctc cttcagacat tcaaagcaca ttcgtataaa tgtgtctttt aaaagtggta
     4861 gtgacctata tgtgctgtga catgcttttt tccctttgta tgtggacatt tcacgttagt
     4921 gcatataggt ctacccattc tttcagtgac tgcttaatag cctactccta atgatggagt
     4981 tgactttcct catacattat gctacactgg cacatgtgca cataaatcgg gagcagcgta
     5041 gttactggga caaaggttac gtatttaaga ctgatggata ttgccaattt tcctccaaaa
     5101 gaaattctcc tgatttacat taatgtaatg taaatgagag tgtttgttcc atcacaacct
     5161 ctcaatgtat tagcagattt cttgattttg aagaaatttt cagaacctgt gctgccacaa
     5221 gtgggcatcc ttttagaaaa gactgttgca atatgttgct tataaaggat ggataataaa
     5281 tagtaaacag gtgatactta gcctgaagaa atgcaggtat aaggtgaagg tactgccctc
     5341 acttttcaag tacattctca aaagataggg gtaacatagc ctgccaaagg cttaaggaaa
     5401 tgcagatatt tattcacaat aataatgtga ctttcttttc cttttttttt tttttttgag
     5461 atggagtttc gctcctgttg cccaggctgg agcgcaacgg cacaatctca gttcactgca
     5521 acctctgcct cccgggttca agcaattctc ctgtctcagc ctccggagta gctgagatta
     5581 caggtgcctg ccaccatgct cggctaattt tttatatttt tagtagagac agtgtttcac
     5641 caagttggcc aggctggtct cgaactcctg accttcaggt gatccacctg cctcagcctc
     5701 ccaaagtgca ggggattaca ggcatgagcc actgcgtctg gcaataatgt aactttgaag
     5761 cttaaaaatt aatcccagtt tgtagcaata acagaagact atctacaacg gaagaaagaa
     5821 gcaactgcct tacagttctg taaagaattg gcaagaaaat aaagcctata gttgccga
//
