LOCUS       NM_005807               5049 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also
            known as V0, mRNA.
ACCESSION   NM_005807
VERSION     NM_005807.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Bagrul,I., Ceylaner,S., Yildiz,Y.T., Tuncez,S., Aydin,E.A.,
            Baglan,E., Ozdel,S. and Bulbul,M.
  TITLE     A novel mutation in the proteoglycan 4 gene causing CACP syndrome:
            two sisters report
  JOURNAL   Pediatr Rheumatol Online J 21 (1), 8 (2023)
   PUBMED   36694203
  REMARK    GeneRIF: A novel mutation in the proteoglycan 4 gene causing CACP
            syndrome: two sisters report.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5049)
  AUTHORS   Schmidt,T.A., Sullivan,D.A., Knop,E., Richards,S.M., Knop,N.,
            Liu,S., Sahin,A., Darabad,R.R., Morrison,S., Kam,W.R. and
            Sullivan,B.D.
  TITLE     Transcription, translation, and function of lubricin, a boundary
            lubricant, at the ocular surface
  JOURNAL   JAMA Ophthalmol 131 (6), 766-776 (2013)
   PUBMED   23599181
  REMARK    GeneRIF: Lubricin is transcribed, translated, and expressed by
            ocular surface epithelia. Lubricin presence significantly reduces
            friction between the cornea and conjunctiva.
REFERENCE   4  (bases 1 to 5049)
  AUTHORS   Rhee,D.K., Marcelino,J., Al-Mayouf,S., Schelling,D.K.,
            Bartels,C.F., Cui,Y., Laxer,R., Goldbach-Mansky,R. and Warman,M.L.
  TITLE     Consequences of disease-causing mutations on lubricin protein
            synthesis, secretion, and post-translational processing
  JOURNAL   J Biol Chem 280 (35), 31325-31332 (2005)
   PUBMED   16000300
  REMARK    GeneRIF: Results describe the production of antibodies against
            human lubricin to determine the consequence of disease-causing
            mutations at the protein level and to study the protein's normal
            post-translational processing.
REFERENCE   5  (bases 1 to 5049)
  AUTHORS   Liu,Y.J., Lu,S.H., Xu,B., Yang,R.C., Ren,Q., Liu,B., Li,B., Lu,M.,
            Yan,F.Y., Han,Z.B. and Han,Z.C.
  TITLE     Hemangiopoietin, a novel human growth factor for the primitive
            cells of both hematopoietic and endothelial cell lineages
  JOURNAL   Blood 103 (12), 4449-4456 (2004)
   PUBMED   14976050
  REMARK    GeneRIF: data suggest that HAPO is a novel growth factor acting on
            the primitive cells of both hematopoietic and endothelial cell
            lineages [HAPO]
REFERENCE   6  (bases 1 to 5049)
  AUTHORS   Jay,G.D., Tantravahi,U., Britt,D.E., Barrach,H.J. and Cha,C.J.
  TITLE     Homology of lubricin and superficial zone protein (SZP): products
            of megakaryocyte stimulating factor (MSF) gene expression by human
            synovial fibroblasts and articular chondrocytes localized to
            chromosome 1q25
  JOURNAL   J Orthop Res 19 (4), 677-687 (2001)
   PUBMED   11518279
REFERENCE   7  (bases 1 to 5049)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   8  (bases 1 to 5049)
  AUTHORS   Marcelino,J., Carpten,J.D., Suwairi,W.M., Gutierrez,O.M.,
            Schwartz,S., Robbins,C., Sood,R., Makalowska,I., Baxevanis,A.,
            Johnstone,B., Laxer,R.M., Zemel,L., Kim,C.A., Herd,J.K., Ihle,J.,
            Williams,C., Johnson,M., Raman,V., Alonso,L.G., Brunoni,D.,
            Gerstein,A., Papadopoulos,N., Bahabri,S.A., Trent,J.M. and
            Warman,M.L.
  TITLE     CACP, encoding a secreted proteoglycan, is mutated in
            camptodactyly-arthropathy-coxa vara-pericarditis syndrome
  JOURNAL   Nat Genet 23 (3), 319-322 (1999)
   PUBMED   10545950
REFERENCE   9  (bases 1 to 5049)
  AUTHORS   Flannery,C.R., Hughes,C.E., Schumacher,B.L., Tudor,D.,
            Aydelotte,M.B., Kuettner,K.E. and Caterson,B.
  TITLE     Articular cartilage superficial zone protein (SZP) is homologous to
            megakaryocyte stimulating factor precursor and Is a multifunctional
            proteoglycan with potential growth-promoting, cytoprotective, and
            lubricating properties in cartilage metabolism
  JOURNAL   Biochem Biophys Res Commun 254 (3), 535-541 (1999)
   PUBMED   9920774
REFERENCE   10 (bases 1 to 5049)
  AUTHORS   Swann,D.A., Silver,F.H., Slayter,H.S., Stafford,W. and Shore,E.
  TITLE     The molecular structure and lubricating activity of lubricin
            isolated from bovine and human synovial fluids
  JOURNAL   Biochem J 225 (1), 195-201 (1985)
   PUBMED   3977823
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133553.9 and KF455089.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 29, 2019 this sequence version replaced NM_005807.5.
            
            Summary: The protein encoded by this gene is a large proteoglycan
            that is synthesized by chondrocytes located at the surface of
            articular cartilage and by some synovial lining cells. This protein
            contains both chondroitin sulfate and keratan sulfate
            glycosaminoglycans. It functions as a boundary lubricant at the
            cartilage surface and contributes to the elastic absorption and
            energy dissipation of synovial fluid. Mutations in this gene result
            in camptodactyly-arthropathy-coxa vara-pericarditis syndrome.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (A, also known as V0) represents
            the longest transcript and encodes the longest isoform (A).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U70136.1 [ECO:0000331]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000445192.7/ ENSP00000399679.3
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AL133553.9         33608-33622         c
            16-121              AL133553.9         32950-33055         c
            122-244             AL133553.9         29688-29810         c
            245-364             AL133553.9         28190-28309         c
            365-514             AL133553.9         25644-25793         c
            515-643             AL133553.9         24979-25107         c
            644-2157            AL133553.9         22070-23583         c
            2158-2309           KF455089.1         1-152               c
            2310-3466           AL133553.9         20761-21917         c
            3467-3544           AL133553.9         20031-20108         c
            3545-3681           AL133553.9         18731-18867         c
            3682-3838           AL133553.9         18305-18461         c
            3839-4036           AL133553.9         17529-17726         c
            4037-4162           AL133553.9         17007-17132         c
            4163-5049           AL133553.9         15334-16220         c
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.1"
     gene            1..5049
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="proteoglycan 4"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
     exon            1..15
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            16..121
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..4260
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="isoform A preproprotein is encoded by transcript
                     variant A, also known as V0; lubricin; megakaryocyte
                     stimulating factor; articular superficial zone protein;
                     superficial zone proteoglycan; hemangiopoietin"
                     /codon_start=1
                     /product="proteoglycan 4 isoform A preproprotein"
                     /protein_id="NP_005798.3"
                     /db_xref="CCDS:CCDS1369.1"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
                     /translation="MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATC
                     NCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYES
                     FCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSV
                     SENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPV
                     VDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSL
                     TVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPK
                     AETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTT
                     PKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKE
                     PAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAP
                     TTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPK
                     EPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKL
                     TPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAP
                     TTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKE
                     PTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPA
                     PKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSE
                     VSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTT
                     AKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFK
                     ITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKK
                     PTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLV
                     EVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNIC
                     NGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNC
                     EGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFF
                     KRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQY
                     SPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNI
                     DVPSRTARAITTRSGQTLSKVWYNCP"
     exon            122..244
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            245..364
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            365..514
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            515..643
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            644..3466
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3467..3544
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3545..3681
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3682..3838
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3839..4036
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            4037..4162
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            4163..5049
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4302..4307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4355
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="major polyA site"
     regulatory      4401..4406
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: ACTAAA"
     polyA_site      4424
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4535..4540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATATA"
     polyA_site      4558
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      5021..5026
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      5049
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
ORIGIN      
        1 atctatcctt tacggcaagg gtacctacgg tacctgaaaa caacgatggc atggaaaaca
       61 cttcccattt acctgttgtt gctgctgtct gttttcgtga ttcagcaagt ttcatctcaa
      121 gatttatcaa gctgtgcagg gagatgtggg gaagggtatt ctagagatgc cacctgcaac
      181 tgtgattata actgtcaaca ctacatggag tgctgccctg atttcaagag agtctgcact
      241 gcggagcttt cctgtaaagg ccgctgcttt gagtccttcg agagagggag ggagtgtgac
      301 tgcgacgccc aatgtaagaa gtatgacaag tgctgtcccg attatgagag tttctgtgca
      361 gaagtgcata atcccacatc accaccatct tcaaagaaag cacctccacc ttcaggagca
      421 tctcaaacca tcaaatcaac aaccaaacgt tcacccaaac caccaaacaa gaagaagact
      481 aagaaagtta tagaatcaga ggaaataaca gaagaacatt ctgtttctga aaatcaagag
      541 tcctcctcct cctcctcctc ttcctcttct tcttcaacaa ttcggaaaat caagtcttcc
      601 aaaaattcag ctgctaatag agaattacag aagaaactca aagtaaaaga taacaagaag
      661 aacagaacta aaaagaaacc tacccccaaa ccaccagttg tagatgaagc tggaagtgga
      721 ttggacaatg gtgacttcaa ggtcacaact cctgacacgt ctaccaccca acacaataaa
      781 gtcagcacat ctcccaagat cacaacagca aaaccaataa atcccagacc cagtcttcca
      841 cctaattctg atacatctaa agagacgtct ttgacagtga ataaagagac aacagttgaa
      901 actaaagaaa ctactacaac aaataaacag acttcaactg atggaaaaga gaagactact
      961 tccgctaaag agacacaaag tatagagaaa acatctgcta aagatttagc acccacatct
     1021 aaagtgctgg ctaaacctac acccaaagct gaaactacaa ccaaaggccc tgctctcacc
     1081 actcccaagg agcccacgcc caccactccc aaggagcctg catctaccac acccaaagag
     1141 cccacaccta ccaccatcaa gtctgcaccc accaccccca aggagcctgc acccaccacc
     1201 accaagtctg cacccaccac tcccaaggag cctgcaccca ccaccaccaa ggagcctgca
     1261 cccaccactc ccaaggagcc tgcacccacc accaccaagg agcctgcacc caccaccacc
     1321 aagtctgcac ccaccactcc caaggagcct gcacccacca cccccaagaa gcctgcccca
     1381 actaccccca aggagcctgc acccaccact cccaaggagc ctacacccac cactcccaag
     1441 gagcctgcac ccaccaccaa ggagcctgca cccaccactc ccaaagagcc tgcacccact
     1501 gcccccaaga agcctgcccc aactaccccc aaggagcctg cacccaccac tcccaaggag
     1561 cctgcaccca ccaccaccaa ggagccttca cccaccactc ccaaggagcc tgcacccacc
     1621 accaccaagt ctgcacccac cactaccaag gagcctgcac ccaccactac caagtctgca
     1681 cccaccactc ccaaggagcc ttcacccacc accaccaagg agcctgcacc caccactccc
     1741 aaggagcctg cacccaccac ccccaagaag cctgccccaa ctacccccaa ggagcctgca
     1801 cccaccactc ccaaggaacc tgcacccacc accaccaaga agcctgcacc caccactccc
     1861 aaagagcctg ccccaactac ccccaaggag actgcaccca ccacccccaa gaagctcacg
     1921 cccaccaccc ccgagaagct cgcacccacc acccctgaga agcccgcacc caccacccct
     1981 gaggagctcg cacccaccac ccctgaggag cccacaccca ccacccctga ggagcctgct
     2041 cccaccactc ccaaggcagc ggctcccaac acccctaagg agcctgctcc aactacccct
     2101 aaggagcctg ctccaactac ccctaaggag cctgctccaa ctacccctaa ggagactgct
     2161 ccaactaccc ctaaagggac tgctccaact accctcaagg aacctgcacc cactactccc
     2221 aagaagcctg cccccaagga gcttgcaccc accaccacca aggagcccac atccaccacc
     2281 tctgacaagc ccgctccaac tacccctaag gggactgctc caactacccc taaggagcct
     2341 gctccaacta cccctaagga gcctgctcca actaccccta aggggactgc tccaactacc
     2401 ctcaaggaac ctgcacccac tactcccaag aagcctgccc ccaaggagct tgcacccacc
     2461 accaccaagg ggcccacatc caccacctct gacaagcctg ctccaactac acctaaggag
     2521 actgctccaa ctacccccaa ggagcctgca cccactaccc ccaagaagcc tgctccaact
     2581 actcctgaga cacctcctcc aaccacttca gaggtctcta ctccaactac caccaaggag
     2641 cctaccacta tccacaaaag ccctgatgaa tcaactcctg agctttctgc agaacccaca
     2701 ccaaaagctc ttgaaaacag tcccaaggaa cctggtgtac ctacaactaa gactcctgca
     2761 gcgactaaac ctgaaatgac tacaacagct aaagacaaga caacagaaag agacttacgt
     2821 actacacctg aaactacaac tgctgcacct aagatgacaa aagagacagc aactacaaca
     2881 gaaaaaacta ccgaatccaa aataacagct acaaccacac aagtaacatc taccacaact
     2941 caagatacca caccattcaa aattactact cttaaaacaa ctactcttgc acccaaagta
     3001 actacaacaa aaaagacaat tactaccact gagattatga acaaacctga agaaacagct
     3061 aaaccaaaag acagagctac taattctaaa gcgacaactc ctaaacctca aaagccaacc
     3121 aaagcaccca aaaaacccac ttctaccaaa aagccaaaaa caatgcctag agtgagaaaa
     3181 ccaaagacga caccaactcc ccgcaagatg acatcaacaa tgccagaatt gaaccctacc
     3241 tcaagaatag cagaagccat gctccaaacc accaccagac ctaaccaaac tccaaactcc
     3301 aaactagttg aagtaaatcc aaagagtgaa gatgcaggtg gtgctgaagg agaaacacct
     3361 catatgcttc tcaggcccca tgtgttcatg cctgaagtta ctcccgacat ggattactta
     3421 ccgagagtac ccaatcaagg cattatcatc aatcccatgc tttccgatga gaccaatata
     3481 tgcaatggta agccagtaga tggactgact actttgcgca atgggacatt agttgcattc
     3541 cgaggtcatt atttctggat gctaagtcca ttcagtccac catctccagc tcgcagaatt
     3601 actgaagttt ggggtattcc ttcccccatt gatactgttt ttactaggtg caactgtgaa
     3661 ggaaaaactt tcttctttaa ggattctcag tactggcgtt ttaccaatga tataaaagat
     3721 gcagggtacc ccaaaccaat tttcaaagga tttggaggac taactggaca aatagtggca
     3781 gcgctttcaa cagctaaata taagaactgg cctgaatctg tgtatttttt caagagaggt
     3841 ggcagcattc agcagtatat ttataaacag gaacctgtac agaagtgccc tggaagaagg
     3901 cctgctctaa attatccagt gtatggagaa acgacacagg ttaggagacg tcgctttgaa
     3961 cgtgctatag gaccttctca aacacacacc atcagaattc aatattcacc tgccagactg
     4021 gcttatcaag acaaaggtgt ccttcataat gaagttaaag tgagtatact gtggagagga
     4081 cttccaaatg tggttacctc agctatatca ctgcccaaca tcagaaaacc tgacggctat
     4141 gattactatg ccttttctaa agatcaatac tataacattg atgtgcctag tagaacagca
     4201 agagcaatta ctactcgttc tgggcagacc ttatccaaag tctggtacaa ctgtccttag
     4261 actgatgagc aaaggaggag tcaactaatg aagaaatgaa taataaattt tgacactgaa
     4321 aaacatttta ttaataaaga atattgacat gagtatacca gtttatatat aaaaatgttt
     4381 ttaaacttga caatcattac actaaaacag atttgataat cttattcaca gttgttattg
     4441 tttacagacc atttaattaa tatttcctct gtttattcct cctctccctc ccattgcatg
     4501 gctcacacct gtaaaagaaa aaagaatcaa attgaatata tcttttaaga attcaaaact
     4561 agtgtattca cttaccctag ttcattataa aaaatatcta ggcattgtgg atataaaact
     4621 gttgggtatt ctacaacttc aatggaaatt attacaagca gattaatccc tctttttgtg
     4681 acacaagtac aatctaaaag ttatattgga aaacatggaa atattaaaat tttacacttt
     4741 tactagctaa aacataatca caaagcttta tcgtgttgta taaaaaaatt aacaatataa
     4801 tggcaatagg tagagataca acaaatgaat ataacactat aacacttcat attttccaaa
     4861 tcttaatttg gatttaagga agaaatcaat aaatataaaa tataagcaca tatttattat
     4921 atatctaagg tatacaaatc tgtctacatg aagtttacag attggtaaat atcacctgct
     4981 caacatgtaa ttatttaata aaactttgga acattaaaaa aataaattgg aggcttaagg
     5041 attagaaaa
//
