LOCUS       NM_001303232            4926 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.
ACCESSION   NM_001303232 XM_005244822
VERSION     NM_001303232.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Bagrul,I., Ceylaner,S., Yildiz,Y.T., Tuncez,S., Aydin,E.A.,
            Baglan,E., Ozdel,S. and Bulbul,M.
  TITLE     A novel mutation in the proteoglycan 4 gene causing CACP syndrome:
            two sisters report
  JOURNAL   Pediatr Rheumatol Online J 21 (1), 8 (2023)
   PUBMED   36694203
  REMARK    GeneRIF: A novel mutation in the proteoglycan 4 gene causing CACP
            syndrome: two sisters report.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4926)
  AUTHORS   Schmidt,T.A., Sullivan,D.A., Knop,E., Richards,S.M., Knop,N.,
            Liu,S., Sahin,A., Darabad,R.R., Morrison,S., Kam,W.R. and
            Sullivan,B.D.
  TITLE     Transcription, translation, and function of lubricin, a boundary
            lubricant, at the ocular surface
  JOURNAL   JAMA Ophthalmol 131 (6), 766-776 (2013)
   PUBMED   23599181
  REMARK    GeneRIF: Lubricin is transcribed, translated, and expressed by
            ocular surface epithelia. Lubricin presence significantly reduces
            friction between the cornea and conjunctiva.
REFERENCE   4  (bases 1 to 4926)
  AUTHORS   Rhee,D.K., Marcelino,J., Al-Mayouf,S., Schelling,D.K.,
            Bartels,C.F., Cui,Y., Laxer,R., Goldbach-Mansky,R. and Warman,M.L.
  TITLE     Consequences of disease-causing mutations on lubricin protein
            synthesis, secretion, and post-translational processing
  JOURNAL   J Biol Chem 280 (35), 31325-31332 (2005)
   PUBMED   16000300
  REMARK    GeneRIF: Results describe the production of antibodies against
            human lubricin to determine the consequence of disease-causing
            mutations at the protein level and to study the protein's normal
            post-translational processing.
REFERENCE   5  (bases 1 to 4926)
  AUTHORS   Liu,Y.J., Lu,S.H., Xu,B., Yang,R.C., Ren,Q., Liu,B., Li,B., Lu,M.,
            Yan,F.Y., Han,Z.B. and Han,Z.C.
  TITLE     Hemangiopoietin, a novel human growth factor for the primitive
            cells of both hematopoietic and endothelial cell lineages
  JOURNAL   Blood 103 (12), 4449-4456 (2004)
   PUBMED   14976050
  REMARK    GeneRIF: data suggest that HAPO is a novel growth factor acting on
            the primitive cells of both hematopoietic and endothelial cell
            lineages [HAPO]
REFERENCE   6  (bases 1 to 4926)
  AUTHORS   Jay,G.D., Tantravahi,U., Britt,D.E., Barrach,H.J. and Cha,C.J.
  TITLE     Homology of lubricin and superficial zone protein (SZP): products
            of megakaryocyte stimulating factor (MSF) gene expression by human
            synovial fibroblasts and articular chondrocytes localized to
            chromosome 1q25
  JOURNAL   J Orthop Res 19 (4), 677-687 (2001)
   PUBMED   11518279
REFERENCE   7  (bases 1 to 4926)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   8  (bases 1 to 4926)
  AUTHORS   Marcelino,J., Carpten,J.D., Suwairi,W.M., Gutierrez,O.M.,
            Schwartz,S., Robbins,C., Sood,R., Makalowska,I., Baxevanis,A.,
            Johnstone,B., Laxer,R.M., Zemel,L., Kim,C.A., Herd,J.K., Ihle,J.,
            Williams,C., Johnson,M., Raman,V., Alonso,L.G., Brunoni,D.,
            Gerstein,A., Papadopoulos,N., Bahabri,S.A., Trent,J.M. and
            Warman,M.L.
  TITLE     CACP, encoding a secreted proteoglycan, is mutated in
            camptodactyly-arthropathy-coxa vara-pericarditis syndrome
  JOURNAL   Nat Genet 23 (3), 319-322 (1999)
   PUBMED   10545950
REFERENCE   9  (bases 1 to 4926)
  AUTHORS   Flannery,C.R., Hughes,C.E., Schumacher,B.L., Tudor,D.,
            Aydelotte,M.B., Kuettner,K.E. and Caterson,B.
  TITLE     Articular cartilage superficial zone protein (SZP) is homologous to
            megakaryocyte stimulating factor precursor and Is a multifunctional
            proteoglycan with potential growth-promoting, cytoprotective, and
            lubricating properties in cartilage metabolism
  JOURNAL   Biochem Biophys Res Commun 254 (3), 535-541 (1999)
   PUBMED   9920774
REFERENCE   10 (bases 1 to 4926)
  AUTHORS   Swann,D.A., Silver,F.H., Slayter,H.S., Stafford,W. and Shore,E.
  TITLE     The molecular structure and lubricating activity of lubricin
            isolated from bovine and human synovial fluids
  JOURNAL   Biochem J 225 (1), 195-201 (1985)
   PUBMED   3977823
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA995681.1, U70136.1,
            AL133553.9 and CA435357.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001303232.1.
            
            Summary: The protein encoded by this gene is a large proteoglycan
            that is synthesized by chondrocytes located at the surface of
            articular cartilage and by some synovial lining cells. This protein
            contains both chondroitin sulfate and keratan sulfate
            glycosaminoglycans. It functions as a boundary lubricant at the
            cartilage surface and contributes to the elastic absorption and
            energy dissipation of synovial fluid. Mutations in this gene result
            in camptodactyly-arthropathy-coxa vara-pericarditis syndrome.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (E, also known as V1) lacks an
            alternate in-frame exon in the 5' coding region, compared to
            variant A. The resulting protein (isoform E) is shorter than
            isoform A.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: PMID: 11518279, 14976050
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-542               DA995681.1         1-542
            543-1731            U70136.1           654-1842
            1732-1732           AL133553.9         22372-22372         c
            1733-3808           U70136.1           1844-3919
            3809-3809           AL133553.9         17633-17633         c
            3810-4915           U70136.1           3921-5026
            4916-4921           CA435357.1         18-23               c
            4922-4926           AL133553.9         15334-15338         c
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.1"
     gene            1..4926
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="proteoglycan 4"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
     exon            1..21
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            22..127
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..4137
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="isoform E preproprotein is encoded by transcript
                     variant E; lubricin; megakaryocyte stimulating factor;
                     articular superficial zone protein; superficial zone
                     proteoglycan; hemangiopoietin"
                     /codon_start=1
                     /product="proteoglycan 4 isoform E preproprotein"
                     /protein_id="NP_001290161.1"
                     /db_xref="CCDS:CCDS81411.1"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
                     /translation="MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATC
                     NCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYES
                     FCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEVKDN
                     KKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPR
                     PSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAK
                     DLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTT
                     PKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEP
                     APTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPT
                     TPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKE
                     PSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPA
                     PTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPT
                     TPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTP
                     KKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAP
                     TTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKK
                     PAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVP
                     TTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATT
                     TQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSK
                     ATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAML
                     QTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQ
                     GIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVW
                     GIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAAL
                     STAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFE
                     RAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPD
                     GYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP"
     exon            128..250
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            251..370
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            371..520
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            521..3343
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3421
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3422..3558
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3715
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..3913
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3914..4039
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            4040..4926
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4179..4184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4232
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="major polyA site"
     regulatory      4278..4283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: ACTAAA"
     polyA_site      4301
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4412..4417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATATA"
     polyA_site      4435
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4898..4903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4926
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
ORIGIN      
        1 aaactcatct atcctttacg gcaagggtac ctacggtacc tgaaaacaac gatggcatgg
       61 aaaacacttc ccatttacct gttgttgctg ctgtctgttt tcgtgattca gcaagtttca
      121 tctcaagatt tatcaagctg tgcagggaga tgtggggaag ggtattctag agatgccacc
      181 tgcaactgtg attataactg tcaacactac atggagtgct gccctgattt caagagagtc
      241 tgcactgcgg agctttcctg taaaggccgc tgctttgagt ccttcgagag agggagggag
      301 tgtgactgcg acgcccaatg taagaagtat gacaagtgct gtcccgatta tgagagtttc
      361 tgtgcagaag tgcataatcc cacatcacca ccatcttcaa agaaagcacc tccaccttca
      421 ggagcatctc aaaccatcaa atcaacaacc aaacgttcac ccaaaccacc aaacaagaag
      481 aagactaaga aagttataga atcagaggaa ataacagaag taaaagataa caagaagaac
      541 agaactaaaa agaaacctac ccccaaacca ccagttgtag atgaagctgg aagtggattg
      601 gacaatggtg acttcaaggt cacaactcct gacacgtcta ccacccaaca caataaagtc
      661 agcacatctc ccaagatcac aacagcaaaa ccaataaatc ccagacccag tcttccacct
      721 aattctgata catctaaaga gacgtctttg acagtgaata aagagacaac agttgaaact
      781 aaagaaacta ctacaacaaa taaacagact tcaactgatg gaaaagagaa gactacttcc
      841 gctaaagaga cacaaagtat agagaaaaca tctgctaaag atttagcacc cacatctaaa
      901 gtgctggcta aacctacacc caaagctgaa actacaacca aaggccctgc tctcaccact
      961 cccaaggagc ccacgcccac cactcccaag gagcctgcat ctaccacacc caaagagccc
     1021 acacctacca ccatcaagtc tgcacccacc acccccaagg agcctgcacc caccaccacc
     1081 aagtctgcac ccaccactcc caaggagcct gcacccacca ccaccaagga gcctgcaccc
     1141 accactccca aggagcctgc acccaccacc accaaggagc ctgcacccac caccaccaag
     1201 tctgcaccca ccactcccaa ggagcctgca cccaccaccc ccaagaagcc tgccccaact
     1261 acccccaagg agcctgcacc caccactccc aaggagccta cacccaccac tcccaaggag
     1321 cctgcaccca ccaccaagga gcctgcaccc accactccca aagagcctgc acccactgcc
     1381 cccaagaagc ctgccccaac tacccccaag gagcctgcac ccaccactcc caaggagcct
     1441 gcacccacca ccaccaagga gccttcaccc accactccca aggagcctgc acccaccacc
     1501 accaagtctg cacccaccac taccaaggag cctgcaccca ccactaccaa gtctgcaccc
     1561 accactccca aggagccttc acccaccacc accaaggagc ctgcacccac cactcccaag
     1621 gagcctgcac ccaccacccc caagaagcct gccccaacta cccccaagga gcctgcaccc
     1681 accactccca aggaacctgc acccaccacc accaagaagc ctgcacccac cactcccaaa
     1741 gagcctgccc caactacccc caaggagact gcacccacca cccccaagaa gctcacgccc
     1801 accacccccg agaagctcgc acccaccacc cctgagaagc ccgcacccac cacccctgag
     1861 gagctcgcac ccaccacccc tgaggagccc acacccacca cccctgagga gcctgctccc
     1921 accactccca aggcagcggc tcccaacacc cctaaggagc ctgctccaac tacccctaag
     1981 gagcctgctc caactacccc taaggagcct gctccaacta cccctaagga gactgctcca
     2041 actaccccta aagggactgc tccaactacc ctcaaggaac ctgcacccac tactcccaag
     2101 aagcctgccc ccaaggagct tgcacccacc accaccaagg agcccacatc caccacctct
     2161 gacaagcccg ctccaactac ccctaagggg actgctccaa ctacccctaa ggagcctgct
     2221 ccaactaccc ctaaggagcc tgctccaact acccctaagg ggactgctcc aactaccctc
     2281 aaggaacctg cacccactac tcccaagaag cctgccccca aggagcttgc acccaccacc
     2341 accaaggggc ccacatccac cacctctgac aagcctgctc caactacacc taaggagact
     2401 gctccaacta cccccaagga gcctgcaccc actaccccca agaagcctgc tccaactact
     2461 cctgagacac ctcctccaac cacttcagag gtctctactc caactaccac caaggagcct
     2521 accactatcc acaaaagccc tgatgaatca actcctgagc tttctgcaga acccacacca
     2581 aaagctcttg aaaacagtcc caaggaacct ggtgtaccta caactaagac tcctgcagcg
     2641 actaaacctg aaatgactac aacagctaaa gacaagacaa cagaaagaga cttacgtact
     2701 acacctgaaa ctacaactgc tgcacctaag atgacaaaag agacagcaac tacaacagaa
     2761 aaaactaccg aatccaaaat aacagctaca accacacaag taacatctac cacaactcaa
     2821 gataccacac cattcaaaat tactactctt aaaacaacta ctcttgcacc caaagtaact
     2881 acaacaaaaa agacaattac taccactgag attatgaaca aacctgaaga aacagctaaa
     2941 ccaaaagaca gagctactaa ttctaaagcg acaactccta aacctcaaaa gccaaccaaa
     3001 gcacccaaaa aacccacttc taccaaaaag ccaaaaacaa tgcctagagt gagaaaacca
     3061 aagacgacac caactccccg caagatgaca tcaacaatgc cagaattgaa ccctacctca
     3121 agaatagcag aagccatgct ccaaaccacc accagaccta accaaactcc aaactccaaa
     3181 ctagttgaag taaatccaaa gagtgaagat gcaggtggtg ctgaaggaga aacacctcat
     3241 atgcttctca ggccccatgt gttcatgcct gaagttactc ccgacatgga ttacttaccg
     3301 agagtaccca atcaaggcat tatcatcaat cccatgcttt ccgatgagac caatatatgc
     3361 aatggtaagc cagtagatgg actgactact ttgcgcaatg ggacattagt tgcattccga
     3421 ggtcattatt tctggatgct aagtccattc agtccaccat ctccagctcg cagaattact
     3481 gaagtttggg gtattccttc ccccattgat actgttttta ctaggtgcaa ctgtgaagga
     3541 aaaactttct tctttaagga ttctcagtac tggcgtttta ccaatgatat aaaagatgca
     3601 gggtacccca aaccaatttt caaaggattt ggaggactaa ctggacaaat agtggcagcg
     3661 ctttcaacag ctaaatataa gaactggcct gaatctgtgt attttttcaa gagaggtggc
     3721 agcattcagc agtatattta taaacaggaa cctgtacaga agtgccctgg aagaaggcct
     3781 gctctaaatt atccagtgta tggagaaacg acacaggtta ggagacgtcg ctttgaacgt
     3841 gctataggac cttctcaaac acacaccatc agaattcaat attcacctgc cagactggct
     3901 tatcaagaca aaggtgtcct tcataatgaa gttaaagtga gtatactgtg gagaggactt
     3961 ccaaatgtgg ttacctcagc tatatcactg cccaacatca gaaaacctga cggctatgat
     4021 tactatgcct tttctaaaga tcaatactat aacattgatg tgcctagtag aacagcaaga
     4081 gcaattacta ctcgttctgg gcagacctta tccaaagtct ggtacaactg tccttagact
     4141 gatgagcaaa ggaggagtca actaatgaag aaatgaataa taaattttga cactgaaaaa
     4201 cattttatta ataaagaata ttgacatgag tataccagtt tatatataaa aatgttttta
     4261 aacttgacaa tcattacact aaaacagatt tgataatctt attcacagtt gttattgttt
     4321 acagaccatt taattaatat ttcctctgtt tattcctcct ctccctccca ttgcatggct
     4381 cacacctgta aaagaaaaaa gaatcaaatt gaatatatct tttaagaatt caaaactagt
     4441 gtattcactt accctagttc attataaaaa atatctaggc attgtggata taaaactgtt
     4501 gggtattcta caacttcaat ggaaattatt acaagcagat taatccctct ttttgtgaca
     4561 caagtacaat ctaaaagtta tattggaaaa catggaaata ttaaaatttt acacttttac
     4621 tagctaaaac ataatcacaa agctttatcg tgttgtataa aaaaattaac aatataatgg
     4681 caataggtag agatacaaca aatgaatata acactataac acttcatatt ttccaaatct
     4741 taatttggat ttaaggaaga aatcaataaa tataaaatat aagcacatat ttattatata
     4801 tctaaggtat acaaatctgt ctacatgaag tttacagatt ggtaaatatc acctgctcaa
     4861 catgtaatta tttaataaaa ctttggaaca ttaaaaaaat aaattggagg cttaaggatt
     4921 agaaaa
//
