LOCUS       NM_001127709            4776 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.
ACCESSION   NM_001127709
VERSION     NM_001127709.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Bagrul,I., Ceylaner,S., Yildiz,Y.T., Tuncez,S., Aydin,E.A.,
            Baglan,E., Ozdel,S. and Bulbul,M.
  TITLE     A novel mutation in the proteoglycan 4 gene causing CACP syndrome:
            two sisters report
  JOURNAL   Pediatr Rheumatol Online J 21 (1), 8 (2023)
   PUBMED   36694203
  REMARK    GeneRIF: A novel mutation in the proteoglycan 4 gene causing CACP
            syndrome: two sisters report.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4776)
  AUTHORS   Schmidt,T.A., Sullivan,D.A., Knop,E., Richards,S.M., Knop,N.,
            Liu,S., Sahin,A., Darabad,R.R., Morrison,S., Kam,W.R. and
            Sullivan,B.D.
  TITLE     Transcription, translation, and function of lubricin, a boundary
            lubricant, at the ocular surface
  JOURNAL   JAMA Ophthalmol 131 (6), 766-776 (2013)
   PUBMED   23599181
  REMARK    GeneRIF: Lubricin is transcribed, translated, and expressed by
            ocular surface epithelia. Lubricin presence significantly reduces
            friction between the cornea and conjunctiva.
REFERENCE   4  (bases 1 to 4776)
  AUTHORS   Rhee,D.K., Marcelino,J., Al-Mayouf,S., Schelling,D.K.,
            Bartels,C.F., Cui,Y., Laxer,R., Goldbach-Mansky,R. and Warman,M.L.
  TITLE     Consequences of disease-causing mutations on lubricin protein
            synthesis, secretion, and post-translational processing
  JOURNAL   J Biol Chem 280 (35), 31325-31332 (2005)
   PUBMED   16000300
  REMARK    GeneRIF: Results describe the production of antibodies against
            human lubricin to determine the consequence of disease-causing
            mutations at the protein level and to study the protein's normal
            post-translational processing.
REFERENCE   5  (bases 1 to 4776)
  AUTHORS   Liu,Y.J., Lu,S.H., Xu,B., Yang,R.C., Ren,Q., Liu,B., Li,B., Lu,M.,
            Yan,F.Y., Han,Z.B. and Han,Z.C.
  TITLE     Hemangiopoietin, a novel human growth factor for the primitive
            cells of both hematopoietic and endothelial cell lineages
  JOURNAL   Blood 103 (12), 4449-4456 (2004)
   PUBMED   14976050
  REMARK    GeneRIF: data suggest that HAPO is a novel growth factor acting on
            the primitive cells of both hematopoietic and endothelial cell
            lineages [HAPO]
REFERENCE   6  (bases 1 to 4776)
  AUTHORS   Jay,G.D., Tantravahi,U., Britt,D.E., Barrach,H.J. and Cha,C.J.
  TITLE     Homology of lubricin and superficial zone protein (SZP): products
            of megakaryocyte stimulating factor (MSF) gene expression by human
            synovial fibroblasts and articular chondrocytes localized to
            chromosome 1q25
  JOURNAL   J Orthop Res 19 (4), 677-687 (2001)
   PUBMED   11518279
REFERENCE   7  (bases 1 to 4776)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   8  (bases 1 to 4776)
  AUTHORS   Marcelino,J., Carpten,J.D., Suwairi,W.M., Gutierrez,O.M.,
            Schwartz,S., Robbins,C., Sood,R., Makalowska,I., Baxevanis,A.,
            Johnstone,B., Laxer,R.M., Zemel,L., Kim,C.A., Herd,J.K., Ihle,J.,
            Williams,C., Johnson,M., Raman,V., Alonso,L.G., Brunoni,D.,
            Gerstein,A., Papadopoulos,N., Bahabri,S.A., Trent,J.M. and
            Warman,M.L.
  TITLE     CACP, encoding a secreted proteoglycan, is mutated in
            camptodactyly-arthropathy-coxa vara-pericarditis syndrome
  JOURNAL   Nat Genet 23 (3), 319-322 (1999)
   PUBMED   10545950
REFERENCE   9  (bases 1 to 4776)
  AUTHORS   Flannery,C.R., Hughes,C.E., Schumacher,B.L., Tudor,D.,
            Aydelotte,M.B., Kuettner,K.E. and Caterson,B.
  TITLE     Articular cartilage superficial zone protein (SZP) is homologous to
            megakaryocyte stimulating factor precursor and Is a multifunctional
            proteoglycan with potential growth-promoting, cytoprotective, and
            lubricating properties in cartilage metabolism
  JOURNAL   Biochem Biophys Res Commun 254 (3), 535-541 (1999)
   PUBMED   9920774
REFERENCE   10 (bases 1 to 4776)
  AUTHORS   Swann,D.A., Silver,F.H., Slayter,H.S., Stafford,W. and Shore,E.
  TITLE     The molecular structure and lubricating activity of lubricin
            isolated from bovine and human synovial fluids
  JOURNAL   Biochem J 225 (1), 195-201 (1985)
   PUBMED   3977823
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA977744.1, DC404857.1,
            AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001127709.2.
            
            Summary: The protein encoded by this gene is a large proteoglycan
            that is synthesized by chondrocytes located at the surface of
            articular cartilage and by some synovial lining cells. This protein
            contains both chondroitin sulfate and keratan sulfate
            glycosaminoglycans. It functions as a boundary lubricant at the
            cartilage surface and contributes to the elastic absorption and
            energy dissipation of synovial fluid. Mutations in this gene result
            in camptodactyly-arthropathy-coxa vara-pericarditis syndrome.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (C, also known as V2) lacks two
            alternate in-frame exons in the 5' coding region, compared to
            variant A. The resulting protein (isoform C) is shorter than
            isoform A.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.1993281.1,
                                           SRR14038194.4321806.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DA977744.1         1-6
            7-563               DC404857.1         1-557
            564-1884            AL133553.9         22070-23390         c
            1885-2036           KF455089.1         1-152               c
            2037-3193           AL133553.9         20761-21917         c
            3194-4239           AK131434.1         2044-3089
            4240-4765           U70136.1           4501-5026
            4766-4771           CA435357.1         18-23               c
            4772-4776           AL133553.9         15334-15338         c
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.1"
     gene            1..4776
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="proteoglycan 4"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
     exon            1..21
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            22..127
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..3987
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="isoform C preproprotein is encoded by transcript
                     variant C; lubricin; megakaryocyte stimulating factor;
                     articular superficial zone protein; superficial zone
                     proteoglycan; hemangiopoietin"
                     /codon_start=1
                     /product="proteoglycan 4 isoform C preproprotein"
                     /protein_id="NP_001121181.2"
                     /db_xref="CCDS:CCDS44287.1"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
                     /translation="MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATC
                     NCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYES
                     FCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKIT
                     TAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQ
                     SIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPT
                     TIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKS
                     APTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPT
                     APKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTK
                     SAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPA
                     PTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPT
                     TPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTL
                     KEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAP
                     TTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKE
                     PAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALEN
                     SPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTT
                     ESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKP
                     KDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPT
                     SRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMD
                     YLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSP
                     ARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGL
                     TGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETT
                     QVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAIS
                     LPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP"
     exon            128..250
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            251..370
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            371..3193
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3271
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3272..3408
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3409..3565
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3566..3763
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..3889
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3890..4776
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4029..4034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4082
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="major polyA site"
     regulatory      4128..4133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: ACTAAA"
     polyA_site      4151
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4262..4267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATATA"
     polyA_site      4285
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4748..4753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4776
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
ORIGIN      
        1 aaactcatct atcctttacg gcaagggtac ctacggtacc tgaaaacaac gatggcatgg
       61 aaaacacttc ccatttacct gttgttgctg ctgtctgttt tcgtgattca gcaagtttca
      121 tctcaagatt tatcaagctg tgcagggaga tgtggggaag ggtattctag agatgccacc
      181 tgcaactgtg attataactg tcaacactac atggagtgct gccctgattt caagagagtc
      241 tgcactgcgg agctttcctg taaaggccgc tgctttgagt ccttcgagag agggagggag
      301 tgtgactgcg acgcccaatg taagaagtat gacaagtgct gtcccgatta tgagagtttc
      361 tgtgcagaag taaaagataa caagaagaac agaactaaaa agaaacctac ccccaaacca
      421 ccagttgtag atgaagctgg aagtggattg gacaatggtg acttcaaggt cacaactcct
      481 gacacgtcta ccacccaaca caataaagtc agcacatctc ccaagatcac aacagcaaaa
      541 ccaataaatc ccagacccag tcttccacct aattctgata catctaaaga gacgtctttg
      601 acagtgaata aagagacaac agttgaaact aaagaaacta ctacaacaaa taaacagact
      661 tcaactgatg gaaaagagaa gactacttcc gctaaagaga cacaaagtat agagaaaaca
      721 tctgctaaag atttagcacc cacatctaaa gtgctggcta aacctacacc caaagctgaa
      781 actacaacca aaggccctgc tctcaccact cccaaggagc ccacgcccac cactcccaag
      841 gagcctgcat ctaccacacc caaagagccc acacctacca ccatcaagtc tgcacccacc
      901 acccccaagg agcctgcacc caccaccacc aagtctgcac ccaccactcc caaggagcct
      961 gcacccacca ccaccaagga gcctgcaccc accactccca aggagcctgc acccaccacc
     1021 accaaggagc ctgcacccac caccaccaag tctgcaccca ccactcccaa ggagcctgca
     1081 cccaccaccc ccaagaagcc tgccccaact acccccaagg agcctgcacc caccactccc
     1141 aaggagccta cacccaccac tcccaaggag cctgcaccca ccaccaagga gcctgcaccc
     1201 accactccca aagagcctgc acccactgcc cccaagaagc ctgccccaac tacccccaag
     1261 gagcctgcac ccaccactcc caaggagcct gcacccacca ccaccaagga gccttcaccc
     1321 accactccca aggagcctgc acccaccacc accaagtctg cacccaccac taccaaggag
     1381 cctgcaccca ccactaccaa gtctgcaccc accactccca aggagccttc acccaccacc
     1441 accaaggagc ctgcacccac cactcccaag gagcctgcac ccaccacccc caagaagcct
     1501 gccccaacta cccccaagga gcctgcaccc accactccca aggaacctgc acccaccacc
     1561 accaagaagc ctgcacccac cactcccaaa gagcctgccc caactacccc caaggagact
     1621 gcacccacca cccccaagaa gctcacgccc accacccccg agaagctcgc acccaccacc
     1681 cctgagaagc ccgcacccac cacccctgag gagctcgcac ccaccacccc tgaggagccc
     1741 acacccacca cccctgagga gcctgctccc accactccca aggcagcggc tcccaacacc
     1801 cctaaggagc ctgctccaac tacccctaag gagcctgctc caactacccc taaggagcct
     1861 gctccaacta cccctaagga gactgctcca actaccccta aagggactgc tccaactacc
     1921 ctcaaggaac ctgcacccac tactcccaag aagcctgccc ccaaggagct tgcacccacc
     1981 accaccaagg agcccacatc caccacctct gacaagcccg ctccaactac ccctaagggg
     2041 actgctccaa ctacccctaa ggagcctgct ccaactaccc ctaaggagcc tgctccaact
     2101 acccctaagg ggactgctcc aactaccctc aaggaacctg cacccactac tcccaagaag
     2161 cctgccccca aggagcttgc acccaccacc accaaggggc ccacatccac cacctctgac
     2221 aagcctgctc caactacacc taaggagact gctccaacta cccccaagga gcctgcaccc
     2281 actaccccca agaagcctgc tccaactact cctgagacac ctcctccaac cacttcagag
     2341 gtctctactc caactaccac caaggagcct accactatcc acaaaagccc tgatgaatca
     2401 actcctgagc tttctgcaga acccacacca aaagctcttg aaaacagtcc caaggaacct
     2461 ggtgtaccta caactaagac tcctgcagcg actaaacctg aaatgactac aacagctaaa
     2521 gacaagacaa cagaaagaga cttacgtact acacctgaaa ctacaactgc tgcacctaag
     2581 atgacaaaag agacagcaac tacaacagaa aaaactaccg aatccaaaat aacagctaca
     2641 accacacaag taacatctac cacaactcaa gataccacac cattcaaaat tactactctt
     2701 aaaacaacta ctcttgcacc caaagtaact acaacaaaaa agacaattac taccactgag
     2761 attatgaaca aacctgaaga aacagctaaa ccaaaagaca gagctactaa ttctaaagcg
     2821 acaactccta aacctcaaaa gccaaccaaa gcacccaaaa aacccacttc taccaaaaag
     2881 ccaaaaacaa tgcctagagt gagaaaacca aagacgacac caactccccg caagatgaca
     2941 tcaacaatgc cagaattgaa ccctacctca agaatagcag aagccatgct ccaaaccacc
     3001 accagaccta accaaactcc aaactccaaa ctagttgaag taaatccaaa gagtgaagat
     3061 gcaggtggtg ctgaaggaga aacacctcat atgcttctca ggccccatgt gttcatgcct
     3121 gaagttactc ccgacatgga ttacttaccg agagtaccca atcaaggcat tatcatcaat
     3181 cccatgcttt ccgatgagac caatatatgc aatggtaagc cagtagatgg actgactact
     3241 ttgcgcaatg ggacattagt tgcattccga ggtcattatt tctggatgct aagtccattc
     3301 agtccaccat ctccagctcg cagaattact gaagtttggg gtattccttc ccccattgat
     3361 actgttttta ctaggtgcaa ctgtgaagga aaaactttct tctttaagga ttctcagtac
     3421 tggcgtttta ccaatgatat aaaagatgca gggtacccca aaccaatttt caaaggattt
     3481 ggaggactaa ctggacaaat agtggcagcg ctttcaacag ctaaatataa gaactggcct
     3541 gaatctgtgt attttttcaa gagaggtggc agcattcagc agtatattta taaacaggaa
     3601 cctgtacaga agtgccctgg aagaaggcct gctctaaatt atccagtgta tggagaaacg
     3661 acacaggtta ggagacgtcg ctttgaacgt gctataggac cttctcaaac acacaccatc
     3721 agaattcaat attcacctgc cagactggct tatcaagaca aaggtgtcct tcataatgaa
     3781 gttaaagtga gtatactgtg gagaggactt ccaaatgtgg ttacctcagc tatatcactg
     3841 cccaacatca gaaaacctga cggctatgat tactatgcct tttctaaaga tcaatactat
     3901 aacattgatg tgcctagtag aacagcaaga gcaattacta ctcgttctgg gcagacctta
     3961 tccaaagtct ggtacaactg tccttagact gatgagcaaa ggaggagtca actaatgaag
     4021 aaatgaataa taaattttga cactgaaaaa cattttatta ataaagaata ttgacatgag
     4081 tataccagtt tatatataaa aatgttttta aacttgacaa tcattacact aaaacagatt
     4141 tgataatctt attcacagtt gttattgttt acagaccatt taattaatat ttcctctgtt
     4201 tattcctcct ctccctccca ttgcatggct cacacctgta aaagaaaaaa gaatcaaatt
     4261 gaatatatct tttaagaatt caaaactagt gtattcactt accctagttc attataaaaa
     4321 atatctaggc attgtggata taaaactgtt gggtattcta caacttcaat ggaaattatt
     4381 acaagcagat taatccctct ttttgtgaca caagtacaat ctaaaagtta tattggaaaa
     4441 catggaaata ttaaaatttt acacttttac tagctaaaac ataatcacaa agctttatcg
     4501 tgttgtataa aaaaattaac aatataatgg caataggtag agatacaaca aatgaatata
     4561 acactataac acttcatatt ttccaaatct taatttggat ttaaggaaga aatcaataaa
     4621 tataaaatat aagcacatat ttattatata tctaaggtat acaaatctgt ctacatgaag
     4681 tttacagatt ggtaaatatc acctgctcaa catgtaatta tttaataaaa ctttggaaca
     4741 ttaaaaaaat aaattggagg cttaaggatt agaaaa
//
