LOCUS       NM_001127708            4932 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.
ACCESSION   NM_001127708
VERSION     NM_001127708.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Zhang,L., Ren,H., Wu,Y., Xue,L., Bai,Y., Wei,D. and Wu,Q.
  TITLE     PRG4 represses the genesis and metastasis of osteosarcoma by
            inhibiting PDL1 expression
  JOURNAL   Tissue Cell 88, 102409 (2024)
   PUBMED   38781792
  REMARK    GeneRIF: PRG4 represses the genesis and metastasis of osteosarcoma
            by inhibiting PDL1 expression.
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Bagrul,I., Ceylaner,S., Yildiz,Y.T., Tuncez,S., Aydin,E.A.,
            Baglan,E., Ozdel,S. and Bulbul,M.
  TITLE     A novel mutation in the proteoglycan 4 gene causing CACP syndrome:
            two sisters report
  JOURNAL   Pediatr Rheumatol Online J 21 (1), 8 (2023)
   PUBMED   36694203
  REMARK    GeneRIF: A novel mutation in the proteoglycan 4 gene causing CACP
            syndrome: two sisters report.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4932)
  AUTHORS   Schmidt,T.A., Sullivan,D.A., Knop,E., Richards,S.M., Knop,N.,
            Liu,S., Sahin,A., Darabad,R.R., Morrison,S., Kam,W.R. and
            Sullivan,B.D.
  TITLE     Transcription, translation, and function of lubricin, a boundary
            lubricant, at the ocular surface
  JOURNAL   JAMA Ophthalmol 131 (6), 766-776 (2013)
   PUBMED   23599181
  REMARK    GeneRIF: Lubricin is transcribed, translated, and expressed by
            ocular surface epithelia. Lubricin presence significantly reduces
            friction between the cornea and conjunctiva.
REFERENCE   4  (bases 1 to 4932)
  AUTHORS   Rhee,D.K., Marcelino,J., Al-Mayouf,S., Schelling,D.K.,
            Bartels,C.F., Cui,Y., Laxer,R., Goldbach-Mansky,R. and Warman,M.L.
  TITLE     Consequences of disease-causing mutations on lubricin protein
            synthesis, secretion, and post-translational processing
  JOURNAL   J Biol Chem 280 (35), 31325-31332 (2005)
   PUBMED   16000300
  REMARK    GeneRIF: Results describe the production of antibodies against
            human lubricin to determine the consequence of disease-causing
            mutations at the protein level and to study the protein's normal
            post-translational processing.
REFERENCE   5  (bases 1 to 4932)
  AUTHORS   Liu,Y.J., Lu,S.H., Xu,B., Yang,R.C., Ren,Q., Liu,B., Li,B., Lu,M.,
            Yan,F.Y., Han,Z.B. and Han,Z.C.
  TITLE     Hemangiopoietin, a novel human growth factor for the primitive
            cells of both hematopoietic and endothelial cell lineages
  JOURNAL   Blood 103 (12), 4449-4456 (2004)
   PUBMED   14976050
  REMARK    GeneRIF: data suggest that HAPO is a novel growth factor acting on
            the primitive cells of both hematopoietic and endothelial cell
            lineages [HAPO]
REFERENCE   6  (bases 1 to 4932)
  AUTHORS   Jay,G.D., Tantravahi,U., Britt,D.E., Barrach,H.J. and Cha,C.J.
  TITLE     Homology of lubricin and superficial zone protein (SZP): products
            of megakaryocyte stimulating factor (MSF) gene expression by human
            synovial fibroblasts and articular chondrocytes localized to
            chromosome 1q25
  JOURNAL   J Orthop Res 19 (4), 677-687 (2001)
   PUBMED   11518279
REFERENCE   7  (bases 1 to 4932)
  AUTHORS   Ikegawa,S., Sano,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Isolation, characterization and mapping of the mouse and human PRG4
            (proteoglycan 4) genes
  JOURNAL   Cytogenet Cell Genet 90 (3-4), 291-297 (2000)
   PUBMED   11124536
REFERENCE   8  (bases 1 to 4932)
  AUTHORS   Marcelino,J., Carpten,J.D., Suwairi,W.M., Gutierrez,O.M.,
            Schwartz,S., Robbins,C., Sood,R., Makalowska,I., Baxevanis,A.,
            Johnstone,B., Laxer,R.M., Zemel,L., Kim,C.A., Herd,J.K., Ihle,J.,
            Williams,C., Johnson,M., Raman,V., Alonso,L.G., Brunoni,D.,
            Gerstein,A., Papadopoulos,N., Bahabri,S.A., Trent,J.M. and
            Warman,M.L.
  TITLE     CACP, encoding a secreted proteoglycan, is mutated in
            camptodactyly-arthropathy-coxa vara-pericarditis syndrome
  JOURNAL   Nat Genet 23 (3), 319-322 (1999)
   PUBMED   10545950
REFERENCE   9  (bases 1 to 4932)
  AUTHORS   Flannery,C.R., Hughes,C.E., Schumacher,B.L., Tudor,D.,
            Aydelotte,M.B., Kuettner,K.E. and Caterson,B.
  TITLE     Articular cartilage superficial zone protein (SZP) is homologous to
            megakaryocyte stimulating factor precursor and Is a multifunctional
            proteoglycan with potential growth-promoting, cytoprotective, and
            lubricating properties in cartilage metabolism
  JOURNAL   Biochem Biophys Res Commun 254 (3), 535-541 (1999)
   PUBMED   9920774
REFERENCE   10 (bases 1 to 4932)
  AUTHORS   Swann,D.A., Silver,F.H., Slayter,H.S., Stafford,W. and Shore,E.
  TITLE     The molecular structure and lubricating activity of lubricin
            isolated from bovine and human synovial fluids
  JOURNAL   Biochem J 225 (1), 195-201 (1985)
   PUBMED   3977823
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA977744.1, AK131434.1,
            AL133553.9, KF455089.1, U70136.1 and CA435357.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001127708.2.
            
            Summary: The protein encoded by this gene is a large proteoglycan
            that is synthesized by chondrocytes located at the surface of
            articular cartilage and by some synovial lining cells. This protein
            contains both chondroitin sulfate and keratan sulfate
            glycosaminoglycans. It functions as a boundary lubricant at the
            cartilage surface and contributes to the elastic absorption and
            energy dissipation of synovial fluid. Mutations in this gene result
            in camptodactyly-arthropathy-coxa vara-pericarditis syndrome.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (B, also known as V0a) lacks an
            alternate in-frame exon in the 5' coding region, compared to
            variant A. The resulting protein (isoform B) is shorter than
            isoform A.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: PMID: 9920774, 11124536
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                DA977744.1         1-50
            51-1159             AK131434.1         35-1143
            1160-2040           AL133553.9         22070-22950         c
            2041-2192           KF455089.1         1-152               c
            2193-2449           AL133553.9         21661-21917         c
            2450-4395           AK131434.1         1144-3089
            4396-4921           U70136.1           4501-5026
            4922-4927           CA435357.1         18-23               c
            4928-4932           AL133553.9         15334-15338         c
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.1"
     gene            1..4932
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="proteoglycan 4"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
     exon            1..21
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            22..127
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..4143
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="isoform B preproprotein is encoded by transcript
                     variant B; lubricin; megakaryocyte stimulating factor;
                     articular superficial zone protein; superficial zone
                     proteoglycan; hemangiopoietin"
                     /codon_start=1
                     /product="proteoglycan 4 isoform B preproprotein"
                     /protein_id="NP_001121180.2"
                     /db_xref="CCDS:CCDS44288.1"
                     /db_xref="GeneID:10216"
                     /db_xref="HGNC:HGNC:9364"
                     /db_xref="MIM:604283"
                     /translation="MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECD
                     CDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKK
                     KTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVK
                     DNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPIN
                     PRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTS
                     AKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAP
                     TTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPK
                     EPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPA
                     PTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTP
                     KEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKE
                     PAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPA
                     PTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPT
                     TPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGT
                     APTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTP
                     KKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPG
                     VPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITA
                     TTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATN
                     SKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEA
                     MLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVP
                     NQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITE
                     VWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVA
                     ALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRR
                     FERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRK
                     PDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP"
     exon            128..247
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            248..397
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            398..526
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            527..3349
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3427
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3564
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3721
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3722..3919
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            3920..4045
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     exon            4046..4932
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4185..4190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4238
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="major polyA site"
     regulatory      4284..4289
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: ACTAAA"
     polyA_site      4307
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4418..4423
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATATA"
     polyA_site      4441
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
     regulatory      4904..4909
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
                     /note="hexamer: AATAAA"
     polyA_site      4932
                     /gene="PRG4"
                     /gene_synonym="CACP; HAPO; JCAP; MSF; SZP"
ORIGIN      
        1 aaactcatct atcctttacg gcaagggtac ctacggtacc tgaaaacaac gatggcatgg
       61 aaaacacttc ccatttacct gttgttgctg ctgtctgttt tcgtgattca gcaagtttca
      121 tctcaagagc tttcctgtaa aggccgctgc tttgagtcct tcgagagagg gagggagtgt
      181 gactgcgacg cccaatgtaa gaagtatgac aagtgctgtc ccgattatga gagtttctgt
      241 gcagaagtgc ataatcccac atcaccacca tcttcaaaga aagcacctcc accttcagga
      301 gcatctcaaa ccatcaaatc aacaaccaaa cgttcaccca aaccaccaaa caagaagaag
      361 actaagaaag ttatagaatc agaggaaata acagaagaac attctgtttc tgaaaatcaa
      421 gagtcctcct cctcctcctc ctcttcctct tcttcttcaa caattcggaa aatcaagtct
      481 tccaaaaatt cagctgctaa tagagaatta cagaagaaac tcaaagtaaa agataacaag
      541 aagaacagaa ctaaaaagaa acctaccccc aaaccaccag ttgtagatga agctggaagt
      601 ggattggaca atggtgactt caaggtcaca actcctgaca cgtctaccac ccaacacaat
      661 aaagtcagca catctcccaa gatcacaaca gcaaaaccaa taaatcccag acccagtctt
      721 ccacctaatt ctgatacatc taaagagacg tctttgacag tgaataaaga gacaacagtt
      781 gaaactaaag aaactactac aacaaataaa cagacttcaa ctgatggaaa agagaagact
      841 acttccgcta aagagacaca aagtatagag aaaacatctg ctaaagattt agcacccaca
      901 tctaaagtgc tggctaaacc tacacccaaa gctgaaacta caaccaaagg ccctgctctc
      961 accactccca aggagcccac gcccaccact cccaaggagc ctgcatctac cacacccaaa
     1021 gagcccacac ctaccaccat caagtctgca cccaccaccc ccaaggagcc tgcacccacc
     1081 accaccaagt ctgcacccac cactcccaag gagcctgcac ccaccaccac caaggagcct
     1141 gcacccacca ctcccaagga gcctgcaccc accaccacca aggagcctgc acccaccacc
     1201 accaagtctg cacccaccac tcccaaggag cctgcaccca ccacccccaa gaagcctgcc
     1261 ccaactaccc ccaaggagcc tgcacccacc actcccaagg agcctacacc caccactccc
     1321 aaggagcctg cacccaccac caaggagcct gcacccacca ctcccaaaga gcctgcaccc
     1381 actgccccca agaagcctgc cccaactacc cccaaggagc ctgcacccac cactcccaag
     1441 gagcctgcac ccaccaccac caaggagcct tcacccacca ctcccaagga gcctgcaccc
     1501 accaccacca agtctgcacc caccactacc aaggagcctg cacccaccac taccaagtct
     1561 gcacccacca ctcccaagga gccttcaccc accaccacca aggagcctgc acccaccact
     1621 cccaaggagc ctgcacccac cacccccaag aagcctgccc caactacccc caaggagcct
     1681 gcacccacca ctcccaagga acctgcaccc accaccacca agaagcctgc acccaccact
     1741 cccaaagagc ctgccccaac tacccccaag gagactgcac ccaccacccc caagaagctc
     1801 acgcccacca cccccgagaa gctcgcaccc accacccctg agaagcccgc acccaccacc
     1861 cctgaggagc tcgcacccac cacccctgag gagcccacac ccaccacccc tgaggagcct
     1921 gctcccacca ctcccaaggc agcggctccc aacaccccta aggagcctgc tccaactacc
     1981 cctaaggagc ctgctccaac tacccctaag gagcctgctc caactacccc taaggagact
     2041 gctccaacta cccctaaagg gactgctcca actaccctca aggaacctgc acccactact
     2101 cccaagaagc ctgcccccaa ggagcttgca cccaccacca ccaaggagcc cacatccacc
     2161 acctctgaca agcccgctcc aactacccct aaggggactg ctccaactac ccctaaggag
     2221 cctgctccaa ctacccctaa ggagcctgct ccaactaccc ctaaggggac tgctccaact
     2281 accctcaagg aacctgcacc cactactccc aagaagcctg cccccaagga gcttgcaccc
     2341 accaccacca aggggcccac atccaccacc tctgacaagc ctgctccaac tacacctaag
     2401 gagactgctc caactacccc caaggagcct gcacccacta cccccaagaa gcctgctcca
     2461 actactcctg agacacctcc tccaaccact tcagaggtct ctactccaac taccaccaag
     2521 gagcctacca ctatccacaa aagccctgat gaatcaactc ctgagctttc tgcagaaccc
     2581 acaccaaaag ctcttgaaaa cagtcccaag gaacctggtg tacctacaac taagactcct
     2641 gcagcgacta aacctgaaat gactacaaca gctaaagaca agacaacaga aagagactta
     2701 cgtactacac ctgaaactac aactgctgca cctaagatga caaaagagac agcaactaca
     2761 acagaaaaaa ctaccgaatc caaaataaca gctacaacca cacaagtaac atctaccaca
     2821 actcaagata ccacaccatt caaaattact actcttaaaa caactactct tgcacccaaa
     2881 gtaactacaa caaaaaagac aattactacc actgagatta tgaacaaacc tgaagaaaca
     2941 gctaaaccaa aagacagagc tactaattct aaagcgacaa ctcctaaacc tcaaaagcca
     3001 accaaagcac ccaaaaaacc cacttctacc aaaaagccaa aaacaatgcc tagagtgaga
     3061 aaaccaaaga cgacaccaac tccccgcaag atgacatcaa caatgccaga attgaaccct
     3121 acctcaagaa tagcagaagc catgctccaa accaccacca gacctaacca aactccaaac
     3181 tccaaactag ttgaagtaaa tccaaagagt gaagatgcag gtggtgctga aggagaaaca
     3241 cctcatatgc ttctcaggcc ccatgtgttc atgcctgaag ttactcccga catggattac
     3301 ttaccgagag tacccaatca aggcattatc atcaatccca tgctttccga tgagaccaat
     3361 atatgcaatg gtaagccagt agatggactg actactttgc gcaatgggac attagttgca
     3421 ttccgaggtc attatttctg gatgctaagt ccattcagtc caccatctcc agctcgcaga
     3481 attactgaag tttggggtat tccttccccc attgatactg tttttactag gtgcaactgt
     3541 gaaggaaaaa ctttcttctt taaggattct cagtactggc gttttaccaa tgatataaaa
     3601 gatgcagggt accccaaacc aattttcaaa ggatttggag gactaactgg acaaatagtg
     3661 gcagcgcttt caacagctaa atataagaac tggcctgaat ctgtgtattt tttcaagaga
     3721 ggtggcagca ttcagcagta tatttataaa caggaacctg tacagaagtg ccctggaaga
     3781 aggcctgctc taaattatcc agtgtatgga gaaacgacac aggttaggag acgtcgcttt
     3841 gaacgtgcta taggaccttc tcaaacacac accatcagaa ttcaatattc acctgccaga
     3901 ctggcttatc aagacaaagg tgtccttcat aatgaagtta aagtgagtat actgtggaga
     3961 ggacttccaa atgtggttac ctcagctata tcactgccca acatcagaaa acctgacggc
     4021 tatgattact atgccttttc taaagatcaa tactataaca ttgatgtgcc tagtagaaca
     4081 gcaagagcaa ttactactcg ttctgggcag accttatcca aagtctggta caactgtcct
     4141 tagactgatg agcaaaggag gagtcaacta atgaagaaat gaataataaa ttttgacact
     4201 gaaaaacatt ttattaataa agaatattga catgagtata ccagtttata tataaaaatg
     4261 tttttaaact tgacaatcat tacactaaaa cagatttgat aatcttattc acagttgtta
     4321 ttgtttacag accatttaat taatatttcc tctgtttatt cctcctctcc ctcccattgc
     4381 atggctcaca cctgtaaaag aaaaaagaat caaattgaat atatctttta agaattcaaa
     4441 actagtgtat tcacttaccc tagttcatta taaaaaatat ctaggcattg tggatataaa
     4501 actgttgggt attctacaac ttcaatggaa attattacaa gcagattaat ccctcttttt
     4561 gtgacacaag tacaatctaa aagttatatt ggaaaacatg gaaatattaa aattttacac
     4621 ttttactagc taaaacataa tcacaaagct ttatcgtgtt gtataaaaaa attaacaata
     4681 taatggcaat aggtagagat acaacaaatg aatataacac tataacactt catattttcc
     4741 aaatcttaat ttggatttaa ggaagaaatc aataaatata aaatataagc acatatttat
     4801 tatatatcta aggtatacaa atctgtctac atgaagttta cagattggta aatatcacct
     4861 gctcaacatg taattattta ataaaacttt ggaacattaa aaaaataaat tggaggctta
     4921 aggattagaa aa
//
