LOCUS       NM_001243245            1398 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens proteoglycan 2, pro eosinophil major basic protein
            (PRG2), transcript variant 2, mRNA.
ACCESSION   NM_001243245
VERSION     NM_001243245.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Futagami,S., Kessoku,T., Kasai,Y., Higurashi,T., Nakajima,A.,
            Agawa,S., Yamawaki,H., Nakamura,K., Habiro,M., Kawawa,R., Ueki,N.,
            Higashida,S., Watanabe,Y., Yamato,H., Yamamoto,T., Takasaki,Y.,
            Ito,K., Hojo,M., Isayama,H., Motoda,N., Ohashi,R., Siah,K.T.H.,
            Ng,C.K. and Gwee,K.A.
  TITLE     Comparison of pancreatic enzyme abnormalities and
            protease-activated receptor-2-positive eosinophils in the duodenum
            of patients with functional dyspepsia-irritable bowel syndrome
            overlap with functional dyspepsia alone in Asian populations
  JOURNAL   J Gastroenterol Hepatol 38 (10), 1778-1786 (2023)
   PUBMED   37278449
  REMARK    GeneRIF: Comparison of pancreatic enzyme abnormalities and
            protease-activated receptor-2-positive eosinophils in the duodenum
            of patients with functional dyspepsia-irritable bowel syndrome
            overlap with functional dyspepsia alone in Asian populations.
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Zhang,E.T., Hannibal,R.L., Badillo Rivera,K.M., Song,J.H.T.,
            McGowan,K., Zhu,X., Meinhardt,G., Knofler,M., Pollheimer,J.,
            Urban,A.E., Folkins,A.K., Lyell,D.J. and Baker,J.C.
  TITLE     PRG2 and AQPEP are misexpressed in fetal membranes in placenta
            previa and percretadagger
  JOURNAL   Biol Reprod 105 (1), 244-257 (2021)
   PUBMED   33982062
  REMARK    GeneRIF: PRG2 and AQPEP are misexpressed in fetal membranes in
            placenta previa and percretadagger.
REFERENCE   3  (bases 1 to 1398)
  AUTHORS   Squillace,D.L., Checkel,J.L., Tefferi,A., Kita,H. and Gleich,G.J.
  TITLE     Development and application of novel immunoassays for eosinophil
            granule major basic proteins to evaluate eosinophilia and
            myeloproliferative disorders
  JOURNAL   J Immunol Methods 493, 113015 (2021)
   PUBMED   33689807
  REMARK    GeneRIF: Development and application of novel immunoassays for
            eosinophil granule major basic proteins to evaluate eosinophilia
            and myeloproliferative disorders.
REFERENCE   4  (bases 1 to 1398)
  AUTHORS   Yamamoto,N., Hiramatsu,N., Isogai,S., Kondo,M., Imaizumi,K. and
            Horiguchi,M.
  TITLE     Mechanism of atopic cataract caused by eosinophil granule major
            basic protein
  JOURNAL   Med Mol Morphol 53 (2), 94-103 (2020)
   PUBMED   31595373
  REMARK    GeneRIF: Mechanism of atopic cataract caused by eosinophil granule
            major basic protein.
REFERENCE   5  (bases 1 to 1398)
  AUTHORS   Al-Jamal,H.A.N., Johan,M.F., Mat Jusoh,S.A., Ismail,I. and Wan
            Taib,W.R.
  TITLE     Re-Expression of Bone Marrow Proteoglycan-2 by 5-Azacytidine is
            associated with STAT3 Inactivation and Sensitivity Response to
            Imatinib in Resistant CML Cells
  JOURNAL   Asian Pac J Cancer Prev 19 (6), 1585-1590 (2018)
   PUBMED   29936783
  REMARK    GeneRIF: Low PRG2 expression is associated with drug resistance in
            Chronic myeloid leukemia.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1398)
  AUTHORS   Yoshimatsu,K., Ohya,Y., Shikata,Y., Seto,T., Hasegawa,Y.,
            Tanaka,I., Kawamura,T., Kitoh,K., Toyoshima,S. and Osawa,T.
  TITLE     Purification and cDNA cloning of a novel factor produced by a human
            T-cell hybridoma: sequence homology with animal lectins
  JOURNAL   Mol Immunol 29 (4), 537-546 (1992)
   PUBMED   1565101
REFERENCE   7  (bases 1 to 1398)
  AUTHORS   Barker,R.L., Loegering,D.A., Arakawa,K.C., Pease,L.R. and
            Gleich,G.J.
  TITLE     Cloning and sequence analysis of the human gene encoding eosinophil
            major basic protein
  JOURNAL   Gene 86 (2), 285-289 (1990)
   PUBMED   2323577
REFERENCE   8  (bases 1 to 1398)
  AUTHORS   Gabay,J.E., Scott,R.W., Campanelli,D., Griffith,J., Wilde,C.,
            Marra,M.N., Seeger,M. and Nathan,C.F.
  TITLE     Antibiotic proteins of human polymorphonuclear leukocytes
  JOURNAL   Proc Natl Acad Sci U S A 86 (14), 5610-5614 (1989)
   PUBMED   2501794
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1989 Dec;86(24):10133]
REFERENCE   9  (bases 1 to 1398)
  AUTHORS   McGrogan,M., Simonsen,C., Scott,R., Griffith,J., Ellis,N.,
            Kennedy,J., Campanelli,D., Nathan,C. and Gabay,J.
  TITLE     Isolation of a complementary DNA clone encoding a precursor to
            human eosinophil major basic protein
  JOURNAL   J Exp Med 168 (6), 2295-2308 (1988)
   PUBMED   3199069
REFERENCE   10 (bases 1 to 1398)
  AUTHORS   Van Noorden,S., Ostberg,Y. and Pearse,A.G.
  TITLE     Localization of somatostatin-like immunoreactivity in the
            pancreatic islets of the hagfish, Myxine glutinosa and the lamprey
            Lampetra fluviatilis
  JOURNAL   Cell Tissue Res 177 (2), 281-285 (1977)
   PUBMED   319906
  REMARK    GeneRIF: This protein contains a peptide that displays strong
            antibacterial activity against Gram-positive bacteria,
            Gram-negative bacteria, and fungi.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000781.6.
            
            On May 31, 2019 this sequence version replaced NM_001243245.2.
            
            Summary: The protein encoded by this gene is the predominant
            constituent of the crystalline core of the eosinophil granule. High
            levels of the proform of this protein are also present in placenta
            and pregnancy serum, where it exists as a complex with several
            other proteins including pregnancy-associated plasma protein A
            (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be
            involved in antiparasitic defense mechanisms as a cytotoxin and
            helminthotoxin, and in immune hypersensitivity reactions. The
            encoded protein contains a peptide that displays potent
            antimicrobial activity against Gram-positive bacteria,
            Gram-negative bacteria, and fungi. It is directly implicated in
            epithelial cell damage, exfoliation, and bronchospasm in allergic
            diseases. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Nov 2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            donor splice site at an internal coding exon compared to variant 1.
            This results in a shorter isoform (2) missing a 11 aa protein
            segment compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB997424.1, BX379838.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162568 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            Protein has antimicrobial activity :: PMID: 3199069
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AP000781.6         145912-145966       c
            56-125              AP000781.6         145203-145272       c
            126-400             AP000781.6         144359-144633       c
            401-532             AP000781.6         143893-144024       c
            533-644             AP000781.6         143070-143181       c
            645-1398            AP000781.6         142096-142849       c
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..1398
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /note="proteoglycan 2, pro eosinophil major basic protein"
                     /db_xref="GeneID:5553"
                     /db_xref="HGNC:HGNC:9362"
                     /db_xref="MIM:605601"
     exon            1..55
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     exon            56..125
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..703
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; natural killer cell activator; bone marrow
                     proteoglycan; eosinophil granule major basic protein;
                     eosinophil major basic protein; proteoglycan 2
                     preproprotein; proteoglycan 2, bone marrow (natural killer
                     cell activator, eosinophil granule major basic protein)"
                     /codon_start=1
                     /product="bone marrow proteoglycan isoform 2
                     preproprotein"
                     /protein_id="NP_001230174.1"
                     /db_xref="CCDS:CCDS58133.1"
                     /db_xref="GeneID:5553"
                     /db_xref="HGNC:HGNC:9362"
                     /db_xref="MIM:605601"
                     /translation="MKLPLLLALLFGAVSALHLRSETSTFETPLGAKTLPEDEETPEQ
                     EMEETPCRELEEEEEWGSGSEDASKKDGAVESISVPDMVDKNLTCPEEEDTVKVVGIP
                     GCQTCRYLLFTCRRCYRGNLVSIHNFNINYRIQCSVSALNQGQVWIGGRITGSGRCRR
                     FQWVDGSRWNFAYWAAHQPWSRGGHCVALCTRGGHWRRAHCLRRLPFICSY"
     exon            126..400
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     exon            401..532
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     exon            533..644
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     exon            645..1398
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      792..797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /note="hexamer: AATAAA"
     polyA_site      813
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /note="major polyA site"
     regulatory      1380..1385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
                     /note="hexamer: AATAAA"
     polyA_site      1398
                     /gene="PRG2"
                     /gene_synonym="BMPG; MBP; MBP1; proMBP"
ORIGIN      
        1 aaagaaggac ctgggctttg ggaagatcta aagacccagg aaggtctctg ggtgggataa
       61 agccaagatg aaactcccct tacttctggc tcttctattt ggggcagttt ctgctcttca
      121 tctaaggtct gagacttcca cctttgagac ccctttgggt gctaagacgc tgcctgagga
      181 tgaggagaca ccagagcagg agatggagga gaccccttgc agggagctgg aggaagagga
      241 ggagtggggc tctggaagtg aagatgcctc caagaaagat ggggctgttg agtctatctc
      301 agtgccagat atggtggaca aaaaccttac gtgtcctgag gaagaggaca cagtaaaagt
      361 ggtgggcatc cctgggtgcc agacctgccg ctacctcctg tttacttgcc ggaggtgcta
      421 caggggcaac ctggtttcca tccacaactt caatattaat tatcgaatcc agtgttctgt
      481 cagcgcgctc aaccagggtc aagtctggat tggaggcagg atcacaggct cgggtcgctg
      541 cagacgcttt cagtgggttg acggcagccg ctggaacttt gcatactggg ctgctcacca
      601 gccctggtcc cgcggtggtc actgcgtggc cctgtgtacc cgaggaggcc actggcgtcg
      661 agcccactgc ctcagaagac ttcctttcat ctgttcctac tgagctggtc ccagccagca
      721 gttcagagct gccctctcct gggcagctgc ctcccctcct ctgcttgcca tccctccctc
      781 cacctccctg caataaaatg ggttttactg aaatggattt attttctcct ctgatcgcgg
      841 atccactctg cttagccctc attgaaactt cttccttatc atctctcccc acaccacaac
      901 tttcatagaa gtgtcagaag ctactactcc ttgaggagga ggatggaggg tggagttggg
      961 tctatggagc cttttggaga tggaggaatg ggctcagcta gttctcttca tagaacacct
     1021 gattactggg cacctgcata gtgctgccag gacctttcaa ggttgtaggt agactcccaa
     1081 tggcccagtt tgcatctctg taaccaaagg ccttttctct ctctctctcc aaccccagaa
     1141 ctgtggttgg ttttatatgt aaggaagtta acatgtccct gggaacagtc cacaacattc
     1201 aggaatgaat gtataagtac cgcaatcccc ggcccctcaa gtggaataaa tctaacatgt
     1261 attgggcacc atttcccagt ggcctgctgt ggtagttggc cttattccat gcatttttat
     1321 gggctgcctt cccttcctca actgcattct ctgctccttc ctactctctg caactcccaa
     1381 ataaacactt gtacgcaa
//
