LOCUS       NM_024870              10824 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens phosphatidylinositol-3,4,5-trisphosphate dependent Rac
            exchange factor 2 (PREX2), transcript variant 1, mRNA.
ACCESSION   NM_024870
VERSION     NM_024870.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Shang,J., Li,Y., Yin,G., Li,Z., Jiang,L. and Bai,Q.
  TITLE     Phosphatidylinositol 3,4,5-Trisphosphate-Dependent Rac Exchanger 2
            Protein Facilitates Glioma Progression via Akt and Stat3 Signaling
  JOURNAL   J Mol Neurosci 71 (8), 1674-1682 (2021)
   PUBMED   34322848
  REMARK    GeneRIF: Phosphatidylinositol 3,4,5-Trisphosphate-Dependent Rac
            Exchanger 2 Protein Facilitates Glioma Progression via Akt and
            Stat3 Signaling.
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Hedayati,M.A., Ahmadi,S., Servatyari,K. and Sheikhesmaeili,F.
  TITLE     PREX2 gene's expression in gastric antral epithelial cells of
            patients with H. pylori infection
  JOURNAL   Arq Gastroenterol 58 (3), 353-358 (2021)
   PUBMED   34705970
  REMARK    GeneRIF: PREX2 gene's expression in gastric antral epithelial cells
            of patients with H. pylori infection.
REFERENCE   3  (bases 1 to 10824)
  AUTHORS   D'Andrea,L., Lucato,C.M., Marquez,E.A., Chang,Y.G., Civciristov,S.,
            Mastos,C., Lupton,C.J., Huang,C., Elmlund,H., Schittenhelm,R.B.,
            Mitchell,C.A., Whisstock,J.C., Halls,M.L. and Ellisdon,A.M.
  TITLE     Structural analysis of the PTEN:P-Rex2 signaling complex reveals
            how cancer-associated mutations coordinate to hyperactivate Rac1
  JOURNAL   Sci Signal 14 (681) (2021)
   PUBMED   33947796
  REMARK    GeneRIF: Structural analysis of the PTEN:P-Rex2 signaling complex
            reveals how cancer-associated mutations coordinate to hyperactivate
            Rac1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10824)
  AUTHORS   Mense,S.M., Barrows,D., Hodakoski,C., Steinbach,N., Schoenfeld,D.,
            Su,W., Hopkins,B.D., Su,T., Fine,B., Hibshoosh,H. and Parsons,R.
  TITLE     PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor
            cell invasion
  JOURNAL   Sci Signal 8 (370), ra32 (2015)
   PUBMED   25829446
  REMARK    GeneRIF: PREX2 mutants are likely selected in cancer to escape
            PTEN-mediated inhibition of invasion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10824)
  AUTHORS   Hodakoski,C., Hopkins,B.D., Barrows,D., Mense,S.M., Keniry,M.,
            Anderson,K.E., Kern,P.A., Hawkins,P.T., Stephens,L.R. and
            Parsons,R.
  TITLE     Regulation of PTEN inhibition by the pleckstrin homology domain of
            P-REX2 during insulin signaling and glucose homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 111 (1), 155-160 (2014)
   PUBMED   24367090
  REMARK    GeneRIF: P-REX2 PH-domain-mediated inhibition of PTEN has a role in
            regulating insulin sensitivity and glucose homeostasis
REFERENCE   6  (bases 1 to 10824)
  AUTHORS   Hernandez-Negrete,I., Carretero-Ortega,J., Rosenfeldt,H.,
            Hernandez-Garcia,R., Calderon-Salinas,J.V., Reyes-Cruz,G.,
            Gutkind,J.S. and Vazquez-Prado,J.
  TITLE     P-Rex1 links mammalian target of rapamycin signaling to Rac
            activation and cell migration
  JOURNAL   J Biol Chem 282 (32), 23708-23715 (2007)
   PUBMED   17565979
REFERENCE   7  (bases 1 to 10824)
  AUTHORS   Joseph,R.E. and Norris,F.A.
  TITLE     Substrate specificity and recognition is conferred by the
            pleckstrin homology domain of the Dbl family guanine nucleotide
            exchange factor P-Rex2
  JOURNAL   J Biol Chem 280 (30), 27508-27512 (2005)
   PUBMED   15897194
  REMARK    GeneRIF: the pleckstrin homology domain of the Dbl family guanine
            nucleotide exchange factor P-Rex2 has a role in substrate
            specificity and recognition
REFERENCE   8  (bases 1 to 10824)
  AUTHORS   Barrios-Rodiles,M., Brown,K.R., Ozdamar,B., Bose,R., Liu,Z.,
            Donovan,R.S., Shinjo,F., Liu,Y., Dembowy,J., Taylor,I.W., Luga,V.,
            Przulj,N., Robinson,M., Suzuki,H., Hayashizaki,Y., Jurisica,I. and
            Wrana,J.L.
  TITLE     High-throughput mapping of a dynamic signaling network in mammalian
            cells
  JOURNAL   Science 307 (5715), 1621-1625 (2005)
   PUBMED   15761153
REFERENCE   9  (bases 1 to 10824)
  AUTHORS   Donald,S., Hill,K., Lecureuil,C., Barnouin,R., Krugmann,S., John
            Coadwell,W., Andrews,S.R., Walker,S.A., Hawkins,P.T., Stephens,L.R.
            and Welch,H.C.
  TITLE     P-Rex2, a new guanine-nucleotide exchange factor for Rac
  JOURNAL   FEBS Lett 572 (1-3), 172-176 (2004)
   PUBMED   15304343
  REMARK    GeneRIF: P-Rex2 is a 183 kDa protein that activates the small
            GTPase Rac and is regulated by phosphatidylinositol
            (3,4,5)-trisphosphate.
REFERENCE   10 (bases 1 to 10824)
  AUTHORS   Rosenfeldt,H., Vazquez-Prado,J. and Gutkind,J.S.
  TITLE     P-REX2, a novel PI-3-kinase sensitive Rac exchange factor
  JOURNAL   FEBS Lett 572 (1-3), 167-171 (2004)
   PUBMED   15304342
  REMARK    GeneRIF: P-REX2 may serve as a novel link between Rac activation
            and the PI-3 kinase pathway.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104416.7, BI457806.1,
            AJ437636.2, CR936666.1, AC103783.2 and R05648.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024870.3.
            
            Summary: The protein encoded by this gene belongs to the
            phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac
            exchanger (PREX) family, which are Dbl-type guanine-nucleotide
            exchange factors for Rac family small G proteins. Structural
            domains of this protein include the catalytic diffuse B-cell
            lymphoma homology and pleckstrin homology (DHPH) domain, two
            disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ
            domains, and a C-terminal inositol polyphosphate-4 phosphatase
            (IP4P) domain that is found in one of the isoforms. This protein
            facilitates the exchange of GDP for GTP on Rac1, allowing the
            GTP-bound Rac1 to activate downstream effectors. Studies also show
            that the pleckstrin homology domain of this protein interacts with
            the phosphatase and tensin homolog (PTEN) gene product to inhibit
            PTEN phosphatase activity, thus activating the phosphoinositide-3
            kinase (PI3K) signaling pathway. Conversely, the PTEN gene product
            has also been shown to inhibit the GEF activity of this protein.
            This gene plays a role in insulin-signaling pathways, and either
            mutations or overexpression of this gene have been observed in some
            cancers. [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.237225.1,
                                           SRR1803613.210938.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288368.5/ ENSP00000288368.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                AC104416.7         80893-80961
            70-72               BI457806.1         8-10
            73-5285             AJ437636.2         1-5213
            5286-6302           CR936666.1         4566-5582           c
            6303-10787          AC103783.2         73971-78455         c
            10788-10824         R05648.1           14-50               c
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.2"
     gene            1..10824
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /note="phosphatidylinositol-3,4,5-trisphosphate dependent
                     Rac exchange factor 2"
                     /db_xref="GeneID:80243"
                     /db_xref="HGNC:HGNC:22950"
                     /db_xref="MIM:612139"
     exon            1..490
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     CDS             350..5170
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /note="isoform a is encoded by transcript variant 1;
                     phosphatidylinositol 3,4,5-trisphosphate-dependent Rac
                     exchanger 2 protein; PtdIns(3,4,5)-dependent Rac exchanger
                     2; DEP domain-containing protein 2; protein phosphatase 1,
                     regulatory subunit 129"
                     /codon_start=1
                     /product="phosphatidylinositol
                     3,4,5-trisphosphate-dependent Rac exchanger 2 protein
                     isoform a"
                     /protein_id="NP_079146.2"
                     /db_xref="CCDS:CCDS6201.1"
                     /db_xref="GeneID:80243"
                     /db_xref="HGNC:HGNC:22950"
                     /db_xref="MIM:612139"
                     /translation="MSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEF
                     LVSAFLHRMNQCAASKVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQ
                     QEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDV
                     PLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQM
                     EKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCK
                     RKHRRLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTA
                     KNKWFVCMAKTPEEKHEWFEAILKERERRKGLKLGMEQDTWVMISEQGEKLYKMMCRQ
                     GNLIKDRKRKLTTFPKCFLGSEFVSWLLEIGEIHRPEEGVHLGQALLENGIIHHVTDK
                     HQFKPEQMLYRFRYDDGTFYPRNEMQDVISKGVRLYCRLHSLFTPVIRDKDYHLRTYK
                     SVVMANKLIDWLIAQGDCRTREEAMIFGVGLCDNGFMHHVLEKSEFKDEPLLFRFFSD
                     EEMEGSNMKHRLMKHDLKVVENVIAKSLLIKSNEGSYGFGLEDKNKVPIIKLVEKGSN
                     AEMAGMEVGKKIFAINGDLVFMRPFNEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPD
                     SADGLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVT
                     ACRKYRRPTKQDSIQWVYNSIESAQEDLQKSHSKPPGDEAGDAFDCKVEEVIDKFNTM
                     AIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMIEPKGFFSLTAKILEAL
                     AKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRISSYKKFSRVLKNRA
                     WPTFKQAKSKISPLHSSDFCPTNCHVNVMEVSYPKTSTSLGSAFGVQLDSRKHNSHDK
                     ENKSSEQGKLSPMVYIQHTITTMAAPSGLSLGQQDGHGLRYLLKEEDLETQDIYQKLL
                     GKLQTALKEVEMCVCQIDDLLSSITYSPKLERKTSEGIIPTDSDNEKGERNSKRVCFN
                     VAGDEQEDSGHDTISNRDSYSDCNSNRNSIASFTSICSSQCSSYFHSDEMDSGDELPL
                     SVRISHDKQDKIHSCLEHLFSQVDSITNLLKGQAVVRAFDQTKYLTPGRGLQEFQQEM
                     EPKLSCPKRLRLHIKQDPWNLPSSVRTLAQNIRKFVEEVKCRLLLALLEYSDSETQLR
                     RDMVFCQTLVATVCAFSEQLMAALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHF
                     QSLLSPNLTDEQAMLEDTLVALFDLEKVSFYFKPSEEEPLVANVPLTYQAEGSRQALK
                     VYFYIDSYHFEQLPQRLKNGGGFKIHPVLFAQALESMEGYYYRDNVSVEEFQAQINAA
                     SLEKVKQYNQKLRAFYLDKSNSPPNSTSKAAYVDKLMRPLNALDELYRLVASFIRSKR
                     TAACANTACSASGVGLLSVSSELCNRLGACHIIMCSSGVHRCTLSVTLEQAIILARSH
                     GLPPRYIMQATDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPAGEE"
     exon            491..562
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            563..685
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            686..790
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            791..892
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1054
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1188
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1292
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1442
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1587
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1688
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1792
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1842
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1918
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..1991
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2134
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2227
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2376
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2462
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2599
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2717
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2902
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3064
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3287
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3288..3495
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3675
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3676..3770
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3771..3853
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3854..3944
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            3945..4073
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4074..4115
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4116..4333
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4436
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4580
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4581..4695
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4696..4762
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4763..4953
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            4954..5056
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            5057..5124
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     exon            5125..10824
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10799..10804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /note="hexamer: AATAAA"
     polyA_site      10824
                     /gene="PREX2"
                     /gene_synonym="DEP.2; DEPDC2; P-REX2; PPP1R129"
                     /note="major polyA site"
ORIGIN      
        1 agcttttgat taatgtccaa gtcactgctg tggctgccga agccgcttcc cctccagccc
       61 cggccgtgtg aggccagagc agcggggccg ggcgcgcagg gcctggccgg agcccccact
      121 cccaggagtt tcctctgcag agccccgcgc cccccgcgcc gggatttcag cccgatcccc
      181 tcctctccct gcgcccagcc tctccccagc atgtaaagtc ttctgtctct cctcggagtt
      241 ggcggaggcg gcaactttcc attctcgccg ccgggggccg ggcagcagcg ggcgcgcggg
      301 tcagcgctca gcacggcggg cagcgccgcg ctgcgcaccg ccgccgacca tgagcgagga
      361 cagccgcgga gacagccgcg ccgagagcgc caaggacctg gagaagcagc ttcgcctgcg
      421 cgtgtgcgtg ctcagcgagc tccagaagac cgagcgggac tatgtgggca cgctggagtt
      481 cctggtgtcg gcattcttac acagaatgaa ccagtgtgca gcatcaaaag ttgacaaaaa
      541 tgtgacagaa gaaacagtga agatgttgtt ctcaaacatt gaagacatcc ttgcagtaca
      601 taaagaattc ttaaaagtcg tggaagaatg cttacacccc gaacctaatg ctcaacaaga
      661 agtgggaacc tgctttcttc actttaaaga caagtttcgt atctatgatg aatattgtag
      721 taaccatgag aaggcacaaa aattacttct tgaactcaac aaaataagaa caatccggac
      781 atttcttttg aactgcatgc tgcttggagg acggaagaac acagatgttc ccttggaagg
      841 atatttagta acaccaatac aaagaatatg caagtaccct cttattttga aggagttgct
      901 gaagcggact ccacggaaac acagtgacta tgcagcagtg atggaagccc tccaagccat
      961 gaaagctgtc tgttccaaca taaacgaggc caagagacag atggagaagt tagaagtttt
     1021 agaggaatgg cagtctcaca ttgaaggctg ggaggggtcc aacatcactg acacctgcac
     1081 tgaaatgcta atgtgtggag tcttactgaa aatttcttct ggaaatattc aagaacgggt
     1141 gttttttctt ttcgataatc ttttggtgta ctgcaaaaga aaacacagac ggttgaagaa
     1201 cagcaaggca tctacagatg gacatcggta cctttttcgt ggccggatca acacggaggt
     1261 gatggaagtg gagaatgtgg atgatggcac cgctgatttc catagcagtg gacacattgt
     1321 tgttaatgga tggaagatac ataacacagc aaaaaataaa tggtttgttt gtatggcaaa
     1381 aacacctgaa gagaagcatg aatggtttga agctattttg aaagaaagag aacggcggaa
     1441 aggtttaaaa ttaggaatgg agcaagatac ctgggtcatg atctctgaac agggtgagaa
     1501 actttataaa atgatgtgca gacaaggaaa tctgatcaaa gaccgaaaga gaaaactgac
     1561 tacgttccct aaatgctttc ttggaagcga atttgtgtca tggctgttgg aaattggaga
     1621 gattcacagg cctgaggaag gcgtgcactt gggacaagca ttattagaaa atggaatcat
     1681 tcaccatgtt actgataaac atcaattcaa accagaacag atgttatata gatttcgcta
     1741 tgatgatgga acattttatc caagaaatga gatgcaggac gtgatttcaa agggtgtaag
     1801 attatattgt cgtcttcata gcctttttac tccagtgata agagataaag attaccattt
     1861 aaggacctac aaatctgtgg tcatggccaa caaactgata gactggttaa ttgcacaggg
     1921 agattgccgc accagagaag aggcaatgat atttggcgtt ggactctgtg acaatggatt
     1981 tatgcaccat gtccttgaaa aaagcgaatt caaagatgaa cccctacttt tccgtttttt
     2041 ttcggatgag gaaatggagg gatcaaatat gaaacatcga cttatgaaac atgacttaaa
     2101 agttgtggaa aatgttatag ctaagtcatt attgattaaa tccaatgaag gcagctatgg
     2161 ctttggatta gaagacaaaa ataaagttcc aataataaag ttggtagaaa agggatctaa
     2221 tgctgagatg gctggcatgg aagtcgggaa aaagattttt gctattaatg gtgacctagt
     2281 ttttatgaga cctttcaatg aagtggattg cttcctgaaa tcgtgtttaa acagcagaaa
     2341 acctctaaga gttcttgtga gcacaaagcc aagagagaca gtgaaaattc cagattcagc
     2401 tgatggactt ggcttccaga tccggggatt tggcccttct gttgtgcatg ctgtaggaag
     2461 aggaactgtg gctgcagcag ctggtcttca ccctggacag tgcattatca aggtgaatgg
     2521 aatcaatgtc agcaaagaga cacatgccag tgtcattgca cacgttacag cctgcaggaa
     2581 gtacaggcgg ccaacgaagc aagattccat acaatgggtt tataatagca ttgagagtgc
     2641 tcaagaagac cttcaaaaat ctcactccaa gccccctgga gatgaagcag gggatgcttt
     2701 tgactgtaaa gtagaagagg ttattgacaa attcaacact atggccatca ttgatgggaa
     2761 gaaggagcat gtgagtctga cagtggacaa tgtccacctg gaatatggtg tcgtgtatga
     2821 gtacgacagc acagctggca tcaagtgcaa tgtggtggaa aagatgattg agcccaaagg
     2881 tttcttcagc ttaactgcca agattcttga agccctggct aaaagtgatg agcattttgt
     2941 acaaaactgt accagcctaa attctctaaa tgaagtgatt cctactgacc ttcagagtaa
     3001 attcagtgcc ctttgcagtg aaagaattga acacctatgt cagagaatat ccagttataa
     3061 aaagttttct cgtgtactga agaatagggc ctggcctact tttaaacagg ccaaatctaa
     3121 aatctcccca ctgcacagca gtgatttctg ccctaccaac tgccatgtca atgtgatgga
     3181 agtttcttat cccaaaacat caacctcttt gggaagtgca tttggtgttc agttggatag
     3241 caggaagcat aattctcatg ataaagaaaa caaatcttcg gagcaaggga aactgagccc
     3301 tatggtgtac attcagcaca ccatcacaac catggcggcc ccttcaggtc tgtctctggg
     3361 acagcaggat ggccatggtc tcaggtatct gctaaaagaa gaagacttag aaacccaaga
     3421 catctatcag aaactgctgg gcaaacttca gactgcactg aaagaggtgg agatgtgtgt
     3481 ttgtcaaata gatgaccttc tgtcttcaat aacatattct cctaaattag aacgtaagac
     3541 atcagagggc ataataccaa cagacagtga caatgagaag ggagaaagaa acagcaaacg
     3601 ggtatgtttt aatgtagcag gagatgaaca ggaagattct ggtcatgaca ccatcagcaa
     3661 cagagactct tacagtgact gcaacagcaa taggaattcc atcgcctcct tcaccagcat
     3721 ctgcagcagc cagtgcagct cgtatttcca cagtgatgaa atggactcag gtgatgaact
     3781 tcccttaagt gttcgcatat ctcatgataa acaggacaag atacatagtt gccttgagca
     3841 tcttttcagc caggtggatt caattaccaa tctcctaaaa gggcaggctg ttgtgagggc
     3901 ctttgaccaa accaagtatc tcactccagg ccgaggattg caagaatttc aacaggaaat
     3961 ggaaccaaag ctgagttgtc caaaaaggct acggcttcat atcaagcaag atccttggaa
     4021 tcttcccagc agcgtccgga ctcttgctca gaacatcagg aaatttgttg aagaggtaaa
     4081 gtgtaggcta ctcctggctc ttcttgaata ttcagatagt gagacacagc tccgtagaga
     4141 catggttttc tgccagactc ttgtggccac tgtctgtgcc ttctctgagc agctcatggc
     4201 ggccttgaac cagatgtttg acaacagcaa ggaaaatgag atggaaactt gggaagccag
     4261 caggaggtgg ctggaccaga tagcgaatgc aggtgttctt tttcactttc agtcacttct
     4321 gtcaccaaac ttgacagatg aacaagccat gttagaagat acactggttg cactatttga
     4381 tttggaaaag gtttcctttt actttaaacc atcagaagag gaacctctgg ttgcaaatgt
     4441 tcctcttaca tatcaagcag aaggaagtcg gcaagctctg aaagtttact tctacattga
     4501 tagttatcat tttgaacaac ttcctcaacg gctgaaaaat ggaggagggt ttaaaattca
     4561 tcctgttctt tttgcacaag cactagagag catggaagga tattattaca gagacaatgt
     4621 ttctgtggaa gaatttcaag ctcagataaa tgcagcctca ctggaaaagg tcaaacagta
     4681 caaccagaag ctcagggcat tctacttgga caagtcaaat tcaccaccaa actccacatc
     4741 caaagctgcc tatgtagata agctaatgag gcctctcaac gctttggatg aactttaccg
     4801 actggtagcc tcgtttatca gatccaagcg cacagctgcc tgtgcaaaca cagcttgcag
     4861 tgcttctggg gttggactgc tgtcagtttc ctcggagctg tgcaacaggc tgggcgcctg
     4921 ccacatcatc atgtgcagca gcggtgtgca tcggtgcacc ctgagcgtga cgctggagca
     4981 agccatcatt ctggccagaa gccacggact gccacctcgt tacatcatgc aggctacaga
     5041 tgtgatgcgg aagcagggag caagagttca gaacacagcg aagaatttgg gagtcagaga
     5101 ccggactcca cagtctgcac caaggctgta caagctgtgc gagccacctc ccccagctgg
     5161 agaagaatga aaagaactcc caagaaacca ggcaggcaga agctcctgaa tgctggacta
     5221 gacaaactac atgctggcta aacattctcc actgaagata catcaatgct tttttttttt
     5281 tttttttctg taaatctttc ttcccattgc aaacaagtag tgatcagttt atactttgat
     5341 atttatgctg gaaatggata gaagttcaat cagacagttc agcttcacga actgatgtct
     5401 ctctgtattg acattaagta ttcactttta gagtaaaatc catccctggg acataaagaa
     5461 aaaaatatta gagatttaaa aattaaccac catgcctcct ctcccattca ttaaaatggt
     5521 tttattatgc ttgttttcat ctttcccaag tggacacatg taataatgga gagttcctct
     5581 tgaagaaggc cattattgaa gaaacctggc gacatcttgc aaatcatcat gagaaatgtt
     5641 aaggacaata tagactgtgt gctatacatt ttcagtcccc catttgcaag agagaaagtc
     5701 attaatgtgt aaaaggtgga aaagcatgct ttactctttc tcctaagggc ttatttctta
     5761 ttagaggaga agaaagctga ctaaggcctc cagcaggact cactttcttc tagctgcctt
     5821 tgggcatcct ttattcttgt ggacagctct agcaaatcaa tgctattttt ggtgtctgca
     5881 atgggcatgg cttccttggg ccatgaatac tgggtgctgt gccataatgg ccctggggat
     5941 cagggaagtg ccctggggat cagggagggg aaccaggatg aagaagggga ctgtaagcct
     6001 attttaaaac taaaccttta agttagatac ctaaaactct taattttatg aaggctctca
     6061 tatgagaggt cagactcttc aaagaataca ccatcatgag aatttttctg ttaagatata
     6121 tgtataagat atgaaaataa ggctttattt gtactttaaa aggaccatta ggggacttgt
     6181 aatttatgga tgcacaaggt gtattctatc cttattctaa agtatatgta atgtttatca
     6241 tttctacatt gatgcaataa cattgttttt agcatagtaa atacagtaat atttatttta
     6301 aatccataat tttcaaacaa aacactcatg attgtgataa ttatatgggt ttacactaat
     6361 ttagtatgtg aaatgctttt catatctaaa ttatgtgcaa actattatta attttttttt
     6421 tattttttga aactgagtct cactctgtta cccagactgg agtgcggtgg tgcaatcttg
     6481 gctcactgca acctccacct cctgggttca agccattctc ttgcctcagc ctcccaggag
     6541 ctgggactac aggtgtgcac caccatgctc agctaatttt tgtattttta gtagagacgt
     6601 gttttcacca tgttggccag gctggtcttg aactcctgac atcaagtgat ccacccacct
     6661 cagcctccca aagtgctagg attacaggcg tgagccgctg ctcccggccc attattaaat
     6721 tttaatttat gaaatttgat catggttttc aaactattat taaaaccttt atcattaacc
     6781 attatgtatt gaataacaaa aataccatgt tacatgtgta ggcaatatgt gtatactact
     6841 aggtaaggta ataataattg ttatttggca cagaagctct agagactttt gtgagaagtc
     6901 catatacaat gaaaaacaag gaacctaaca tcagagtttt caagaaaaag ttgaacattt
     6961 tgaatttaat aaagatatct ttacaaaaat aaccttcaag gatttaacat ttgtaggact
     7021 aataaaggaa cacagctgat tagctagcaa gtcctttcat attgtcatga ctgttttctc
     7081 atcaccatcc tggaagcagt aatcctcatt agaggcatgc aagggtcatt aaaatgattt
     7141 cagttgtact ttgccatatt tcatggggca aattatcagc tatattgtgg tatttataag
     7201 ctaacatatt ttatattttt aaggatctac tttcttcata atagaatgtg cttcatatta
     7261 tttttagggt taaacataag tgtgtcgtct atcatgcttg gtacttacta aatgctcaaa
     7321 aattagtaat ggtttatatt aacatcagtc actctaactt ctgatcgtaa taaaaattgt
     7381 agctaacact tattaaggga ttactacatg cccagtaata cctccaaatg ctttgcctgt
     7441 attctgttgt tattgcttca cttaatcctc ataataatga cgtgacatgg tactcttttt
     7501 atcgcgccca tttttagagt tgaggaacca aagcaaagaa taacccagtg acttgcacaa
     7561 agtcacatga ttgacaaggg gtagaaccaa aatttgaacc caggaagtct gccttcttaa
     7621 tgcattatgc tacttttcct tatcaatata agaactattg taatattacg atgatttgta
     7681 agtaaatttg gagttatgcg gcatgtgttg tcattaaatt atacatcttg tattaaaaaa
     7741 cggtgaatgg aaatatgtaa atgctttaat tatatgaaaa ttatagtgtt tcctggacat
     7801 tttacaactc tggatgcaca ttttgaggta actcaacagt gttgcaatct gaaaaactat
     7861 tttgtatcat gttcctcctc atcaaaaata acagttttag atagtacatc tcatacaact
     7921 ttattataat tccaacacag tggtaagagt aaaaaacata cctaatggat gaatgaataa
     7981 tgacaatttt ttcaatgcca tatgtacctc catgtgacat tgggcattaa atggccttga
     8041 ggttttagga tgtgtttacc ttaatgctac atgctcctct ataaccagtg caccttcaaa
     8101 tactgctaaa acctgctaac ttcttccagg ttctgagcat ggacaactcc caccgccccc
     8161 caacctaaaa gggaaggtca tgcatagaaa gtttggattt taacttagag cctgactcaa
     8221 aaataattgt gaactaaaaa ttcatagtca atacttttta agcattctat tatctcctat
     8281 aattttttct aatattaatt ccaaatatta caaaatttaa tgtgatatta aagtatgaaa
     8341 aatttgagaa gattagaatt ttacatgtat ttccattcct atttaaagag agaaaatggg
     8401 gagataattt ctgtgctagt tttacttata ttggatatat aatattgaat ttatagtata
     8461 agctttattt tatcagttcg caaatctgaa gtctcatact gttttctgtc tctttaaaac
     8521 acattcttct ggtggcttgg gttcattgtg cattcattgg ccactcttct tgcagcaaat
     8581 tttaaatcaa tttaaaatta ggtaaacttt ttatgatact ataatatata tgacgttgaa
     8641 atgacattgg ttctgagtct attaatttaa attttgggat cattacattc agaatgaaat
     8701 gaaattgacc ttgatactaa ctttgagagc caaaaaaatg aatgggtaaa caaagcctaa
     8761 gggtcatgtt aattaattta acaaaacaca ctattttcag tcctttgggc ttgtacattt
     8821 tatagacaaa attagcattt attgatttgg aagtgttcaa ttgagagtaa atgactctgt
     8881 gttttgaaac taataccttt gcatttaaaa tactgaagta ttattttgtt agttgaagta
     8941 gatcttttcc tgtgtagcat gatttaatgc agagcagtta ttcacttata cttctgaaaa
     9001 gccataatat tgaaatttaa aatgaatagt cttactttaa ggtcactttt actttacatt
     9061 ttactttaaa ggtcgttttt agactttatg cactagtggt attgaaacta acctttaata
     9121 tttgactaaa ttgcttgtga atttttggaa actatgttta atttttaaag acattgttat
     9181 gtgaattatc ttactaaatt atcacttaag aactctctca tagcagtaat ttcattaaca
     9241 catatatata ctaaccaatt tgctaaatga attgtcggtt gtcctgctta agtactaggg
     9301 ccaagctttg aaatgactta aagtgatctg aattttgatt gagaaaaata atctgtcttc
     9361 tatcgaatgt ttgaactttt agtgatgagc ctcagcaaag tttcaccctg catgaagcag
     9421 aacaggtggg ggaaaattac aagaggggtc tgtaccaaaa atggccccag gatattccat
     9481 tgaacaaata ttgaccatta tcttcctagg tgctacaact ccatactagt cattacagtt
     9541 agtctttggt ttcacaaaaa tgttactgct tcaaaggctt ggcattaaca gtgatgtcag
     9601 cattcatact gccaacagtc tgctggtcat acatggaaga ctgttaaagt gtggtctgtg
     9661 catcattgag caattgttat gtagacacaa ggcctgcttc actcaggata gcttgctcct
     9721 ctcgctgaga agagatacct gaagtctact aagtctacta aatgagatat atatgtgtat
     9781 gtatatgcct atatatatta ttatatttac tagtgtttgt gataatgtta taaaaattaa
     9841 taagattgtt ttgttttgta tgaatgtacg acaaatcgag ttgttcaatg tattgaatga
     9901 atgtcttact gtaagcatca gagctgtcca accatgtgta aatgtacctg gtcttttgat
     9961 cactgacact actttgctgt tcaatctgga atcaactgac ctggctctgc cattgagaaa
    10021 taactaattt agaacaaaat tgttaaattt taaaattgca tttctactag aaatagcttt
    10081 ggagagttaa ttttaggtgc gaataacagg ataaatgcta tctgatcaag cattcatccg
    10141 aaaaatgttt ttgagaattc actttgtgca aggcacagct gttggggctg gtatatgtgt
    10201 ggacatgttt gatttgaaaa ccttcgtggt gtctctgtgt gttattgatc atctgtgtct
    10261 tttgcttaaa atggcattat ttgtaacaca aacctaatat catatataca aatatactgt
    10321 atgtagccaa gaacactaaa ttggtaagac gctttagaaa atatgcattt gaatttgaat
    10381 atctaatgcc tcaagcaaat ttattagagt ttcagatgaa tatatatttt tgtaactggc
    10441 aagtaaatga gtagaacaga gctttttgcc taaaaatatt tttataagta ctcatttaca
    10501 tagtattcat tattttaaat gacaaaatat caaattggtt ctataaatag taattgtttt
    10561 gaatatatcc atacttaaca tcatatgaaa tccaaaattt aatgataaat ttcatctgga
    10621 tgtgttatta taaaagtcat ctttttttcc tttgtaaaat atccatgtaa catagcttcc
    10681 attactccat attttatgta tggttaaaat tatcaaagtg cattatgtat tttatattgt
    10741 ttatttttgt ctgcatgaaa gattggaata gtaaccgtgt atgataaatg ttaataataa
    10801 taaagtgaaa tgtagtatga ccaa
//
