LOCUS       NM_020820               6752 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens phosphatidylinositol-3,4,5-trisphosphate dependent Rac
            exchange factor 1 (PREX1), mRNA.
ACCESSION   NM_020820
VERSION     NM_020820.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ravala,S.K., Adame-Garcia,S.R., Li,S., Chen,C.L., Cianfrocco,M.A.,
            Silvio Gutkind,J., Cash,J.N. and Tesmer,J.J.G.
  TITLE     Structural and dynamic changes in P-Rex1 upon activation by PIP3
            and inhibition by IP4
  JOURNAL   Elife 12, 92822 (2024)
   PUBMED   39082940
  REMARK    GeneRIF: Structural and dynamic changes in P-Rex1 upon activation
            by PIP3 and inhibition by IP4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Zhang,H., Okuyama,H., Jain,A., Jadhav,R.R., Wu,B., Sturmlechner,I.,
            Morales,J., Ohtsuki,S., Weyand,C.M. and Goronzy,J.J.
  TITLE     PREX1 improves homeostatic proliferation to maintain a naive CD4+ T
            cell compartment in older age
  JOURNAL   JCI Insight 9 (5), e172848 (2024)
   PUBMED   38329813
  REMARK    GeneRIF: PREX1 improves homeostatic proliferation to maintain a
            naive CD4+ T cell compartment in older age.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6752)
  AUTHORS   Gao,X., Lin,X., Lin,M., Lan,Y., Wang,Y., Wu,R., Li,J., Huang,C. and
            Zhong,D.
  TITLE     Silencing Rac1 and Prex1 Inhibit Epithelial-Mesenchymal Transition
            in Human Gastric Cancer Cells Induced by Transforming Growth
            Factor-beta1
  JOURNAL   Turk J Gastroenterol 34 (9), 975-981 (2023)
   PUBMED   37434402
  REMARK    GeneRIF: Silencing Rac1 and Prex1 Inhibit Epithelial-Mesenchymal
            Transition in Human Gastric Cancer Cells Induced by Transforming
            Growth Factor-beta1.
REFERENCE   4  (bases 1 to 6752)
  AUTHORS   Chang,Y.G., Lupton,C.J., Bayly-Jones,C., Keen,A.C., D'Andrea,L.,
            Lucato,C.M., Steele,J.R., Venugopal,H., Schittenhelm,R.B.,
            Whisstock,J.C., Halls,M.L. and Ellisdon,A.M.
  TITLE     Structure of the metastatic factor P-Rex1 reveals a two-layered
            autoinhibitory mechanism
  JOURNAL   Nat Struct Mol Biol 29 (8), 767-773 (2022)
   PUBMED   35864164
  REMARK    GeneRIF: Structure of the metastatic factor P-Rex1 reveals a
            two-layered autoinhibitory mechanism.
REFERENCE   5  (bases 1 to 6752)
  AUTHORS   Lavictoire,S.J., Jomaa,D., Gont,A., Jardine,K., Cook,D.P. and
            Lorimer,I.A.J.
  TITLE     Identification of Rac guanine nucleotide exchange factors promoting
            Lgl1 phosphorylation in glioblastoma
  JOURNAL   J Biol Chem 297 (5), 101172 (2021)
   PUBMED   34624316
  REMARK    GeneRIF: Identification of Rac guanine nucleotide exchange factors
            promoting Lgl1 phosphorylation in glioblastoma.
REFERENCE   6  (bases 1 to 6752)
  AUTHORS   Weiner,O.D.
  TITLE     Rac activation: P-Rex1 - a convergence point for PIP(3) and
            Gbetagamma?
  JOURNAL   Curr Biol 12 (12), R429-R431 (2002)
   PUBMED   12123595
  REMARK    GeneRIF: P-Rex1 is synergistically activated by PIP(3) and
            Gbetagamma and may act as a coincidence detector for these
            signaling molecules
REFERENCE   7  (bases 1 to 6752)
  AUTHORS   Welch,H.C., Coadwell,W.J., Ellson,C.D., Ferguson,G.J.,
            Andrews,S.R., Erdjument-Bromage,H., Tempst,P., Hawkins,P.T. and
            Stephens,L.R.
  TITLE     P-Rex1, a PtdIns(3,4,5)P3- and Gbetagamma-regulated
            guanine-nucleotide exchange factor for Rac
  JOURNAL   Cell 108 (6), 809-821 (2002)
   PUBMED   11955434
  REMARK    GeneRIF: P-Rex1 appears to be a coincidence detector in
            PtdIns(3,4,5)P3 and Gbetagamma signaling pathways that is
            particularly adapted to function downstream of heterotrimeric G
            proteins in neutrophils.
REFERENCE   8  (bases 1 to 6752)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 6752)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   10 (bases 1 to 6752)
  AUTHORS   Fossey,S.C., Mychaleckyj,J.C., Pendleton,J.K., Snyder,J.R.,
            Bensen,J.T., Hirakawa,S., Rich,S.S., Freedman,B.I. and Bowden,D.W.
  TITLE     A high-resolution 6.0-megabase transcript map of the type 2
            diabetes susceptibility region on human chromosome 20
  JOURNAL   Genomics 76 (1-3), 45-57 (2001)
   PUBMED   11549316
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133342.14, AL035106.28 and
            AL445192.3.
            
            On Nov 23, 2018 this sequence version replaced NM_020820.3.
            
            Summary: The protein encoded by this gene acts as a guanine
            nucleotide exchange factor for the RHO family of small GTP-binding
            proteins (RACs).  It has been shown to bind to and activate RAC1 by
            exchanging bound GDP for free GTP. The encoded protein, which is
            found mainly in the cytoplasm, is activated by
            phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma
            subunits of heterotrimeric G proteins. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB037836.2, AJ320261.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371941.4/ ENSP00000361009.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               AL133342.14        48856-49213         c
            359-430             AL035106.28        99930-100001        c
            431-553             AL035106.28        97146-97268         c
            554-658             AL035106.28        86667-86771         c
            659-760             AL035106.28        78411-78512         c
            761-922             AL035106.28        60382-60543         c
            923-1056            AL035106.28        52875-53008         c
            1057-1175           AL035106.28        44794-44912         c
            1176-1325           AL035106.28        43069-43218         c
            1326-1473           AL035106.28        40779-40926         c
            1474-1574           AL035106.28        33357-33457         c
            1575-1678           AL035106.28        31773-31876         c
            1679-1728           AL035106.28        31482-31531         c
            1729-1804           AL035106.28        28315-28390         c
            1805-1877           AL035106.28        18405-18477         c
            1878-2020           AL035106.28        12041-12183         c
            2021-2113           AL035106.28        10258-10350         c
            2114-2262           AL035106.28        9162-9310           c
            2263-2348           AL035106.28        7412-7497           c
            2349-2485           AL035106.28        5483-5619           c
            2486-2606           AL035106.28        4708-4828           c
            2607-2794           AL035106.28        3518-3705           c
            2795-2956           AL035106.28        3016-3177           c
            2957-3167           AL035106.28        2118-2328           c
            3168-3444           AL035106.28        1422-1698           c
            3445-3651           AL445192.3         73827-74033         c
            3652-3740           AL445192.3         72385-72473         c
            3741-3823           AL445192.3         70383-70465         c
            3824-3914           AL445192.3         70144-70234         c
            3915-4043           AL445192.3         67742-67870         c
            4044-4085           AL445192.3         65687-65728         c
            4086-4306           AL445192.3         64439-64659         c
            4307-4406           AL445192.3         62652-62751         c
            4407-4550           AL445192.3         60472-60615         c
            4551-4665           AL445192.3         60253-60367         c
            4666-4732           AL445192.3         58704-58770         c
            4733-4905           AL445192.3         57425-57597         c
            4906-5008           AL445192.3         55837-55939         c
            5009-5076           AL445192.3         55524-55591         c
            5077-6752           AL445192.3         52228-53903         c
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..6752
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /note="phosphatidylinositol-3,4,5-trisphosphate dependent
                     Rac exchange factor 1"
                     /db_xref="GeneID:57580"
                     /db_xref="HGNC:HGNC:32594"
                     /db_xref="MIM:606905"
     exon            1..358
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..5119
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /note="ptdIns(3,4,5)-dependent Rac exchanger 1; PIP3
                     dependent Rac exchange factor 1"
                     /codon_start=1
                     /product="phosphatidylinositol
                     3,4,5-trisphosphate-dependent Rac exchanger 1 protein"
                     /protein_id="NP_065871.3"
                     /db_xref="CCDS:CCDS13410.1"
                     /db_xref="GeneID:57580"
                     /db_xref="HGNC:HGNC:32594"
                     /db_xref="MIM:606905"
                     /translation="MEAPSGSEPGGDGAGDCAHPDPRAPGAAAPSSGPGPCAAARESE
                     RQLRLRLCVLNEILGTERDYVGTLRFLQSAFLHRIRQNVADSVEKGLTEENVKVLFSN
                     IEDILEVHKDFLAALEYCLHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLV
                     ELNKIPTVRAFLLSCMLLGGRKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHP
                     DHPAVQSALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQG
                     TLLKISAGNIQERAFFLFDNLLVYCKRKSRVTGSKKSTKRTKSINGSLYIFRGRINTE
                     VMEVENVEDGTADYHSNGYTVTNGWKIHNTAKNKWFVCMAKTAEEKQKWLDAIIRERE
                     QRESLKLGMERDAYVMIAEKGEKLYHMMMNKKVNLIKDRRRKLSTVPKCFLGNEFVAW
                     LLEIGEISKTEEGVNLGQALLENGIIHHVSDKHQFKNEQVMYRFRYDDGTYKARSELE
                     DIMSKGVRLYCRLHSLYTPVIKDRDYHLKTYKSVLPGSKLVDWLLAQGDCQTREEAVA
                     LGVGLCNNGFMHHVLEKSEFRDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILAK
                     RLLILPQEEDYGFDIEEKNKAVVVKSVQRGSLAEVAGLQVGRKIYSINEDLVFLRPFS
                     EVESILNQSFCSRRPLRLLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEA
                     MAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEA
                     RASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVV
                     YEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVENCRRLMALSSAIVTM
                     PHFEFRNICDTKLESIGQRIACYQEFAAQLKSRVSPPFKQAPLEPHPLCGLDFCPTNC
                     HINLMEVSYPKTTPSVGRSFSIRFGRKPSLIGLDPEQGHLNPMSYTQHCITTMAAPSW
                     KCLPAAEGDPQGQGLHDGSFGPASGTLGQEDRGLSFLLKQEDREIQDAYLQLFTKLDV
                     ALKEMKQYVTQINRLLSTITEPTSGGSCDASLAEEASSLPLVSEESEMDRSDHGGIKK
                     VCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGSGDEL
                     PCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKHFPMNHSLQEFKQ
                     KEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLLALLKCTDTELQ
                     LRRDAIFCQALVAAVCTFSKQLLAALGYRYNNNGEYEESSRDASRKWLEQVAATGVLL
                     HCQSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVFYHIEGSRQA
                     LKVIFYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAAEDLQQDIN
                     AQSLEKVQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRLMKSFVHP
                     KPGAAGSVGAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILARSHGLLP
                     KCIMQATDIMRKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL"
     exon            359..430
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            431..553
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            554..658
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..760
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            761..922
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1056
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1175
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1325
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1473
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1574
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1678
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1728
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1804
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1877
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..2020
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2113
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2262
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2348
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2485
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2606
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..2794
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2795..2956
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3167
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3168..3444
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3651
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3740
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3741..3823
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..3914
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3915..4043
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4044..4085
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4086..4306
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4307..4406
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4407..4550
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4551..4665
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4666..4732
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4733..4905
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            4906..5008
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            5009..5076
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     exon            5077..6752
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6728..6733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /note="hexamer: ATTAAA"
     polyA_site      6752
                     /gene="PREX1"
                     /gene_synonym="P-REX1"
                     /note="major polyA site"
ORIGIN      
        1 agtcccgccc gccgcgccgg gagcccggcc cgccgctgag cccggcccgc cggcgcgccc
       61 cgtccgggat ggggcgcctg gacgcctgag ttgccgctcg gcgcgacgga aggagccggc
      121 gccgcgcggc cgcgctagaa tggaggcgcc cagcggcagc gagcccggcg gcgacggggc
      181 cggggactgc gcccacccgg acccccgggc ccctggcgcc gcggcgccca gctccggccc
      241 cggcccgtgc gcggccgccc gggagtccga gcgccagctg cgcctccgcc tctgcgtcct
      301 caacgagatc ttgggcaccg agagggacta cgtgggcacc ttgcgcttct tgcagtcggc
      361 attcctgcat cgcatccggc agaacgtggc cgactcagtg gagaagggcc tcacggagga
      421 gaatgtcaag gtcctgttct cgaacatcga agacatcctg gaagttcata aggatttctt
      481 ggccgccttg gagtattgtt tacacccgga gccgcagtct cagcatgaac ttgggaatgt
      541 tttcttaaaa ttcaaggaca agttctgcgt gtacgaggag tattgcagca accatgagaa
      601 agccctgagg ctgctggtgg agctgaacaa gatccctacc gtgcgcgcct tccttttgag
      661 ctgcatgctt ctgggaggcc ggaagaccac ggacatccct ttggaaggct acctgttgtc
      721 tccgatccag aggatctgca agtacccgct cctccttaag gagctggcca agaggactcc
      781 cggcaagcac ccagaccacc ccgcggtcca gagtgccctg caggccatga agaccgtttg
      841 ctccaacatc aatgagacca agcggcagat ggagaagctg gaagccctgg agcagctgca
      901 gtcccacatc gaaggctggg agggttccaa cctcacagac atctgcactc agctcctcct
      961 gcaagggact ttgttaaaga tctctgcggg caacatccag gaaagggcct tcttcctctt
     1021 cgacaacctt ctcgtctact gcaagcggaa atccagggtc accgggagca agaagtccac
     1081 caagaggacc aaatccatca acggctccct ctacatcttc aggggtcgaa tcaacactga
     1141 agtcatggag gtggagaatg tggaagatgg gacagcggat taccatagca acggctatac
     1201 cgtcaccaac ggctggaaga tccacaacac ggccaagaat aagtggtttg tctgcatggc
     1261 caagacggca gaggagaagc agaagtggct ggatgccatc atccgcgagc gggagcagcg
     1321 cgagagcctg aagctgggca tggagcgtga tgcctacgtc atgattgcgg agaaggggga
     1381 gaagctgtac cacatgatga tgaacaagaa ggtgaacctc atcaaggacc gccggagaaa
     1441 gctgagcact gtccccaagt gctttcttgg caatgagttc gttgcctggc tcctagaaat
     1501 tggtgaaatc agcaagacgg aagaaggagt caacttgggc caagccctgt tggagaatgg
     1561 catcatccac catgtttccg acaagcacca gttcaagaat gagcaggtga tgtatcgctt
     1621 ccgctacgac gatggcacct acaaggcccg aagtgagctg gaggacatca tgtccaaggg
     1681 tgtgaggctt tactgccgtc ttcacagcct ctacaccccg gtgatcaaag accgtgatta
     1741 ccacctgaag acctacaagt cagtgcttcc cgggagcaag ctggtggact ggctgctggc
     1801 tcagggagac tgccagactc gggaggaggc agtggcgctc ggcgtgggtc tgtgcaacaa
     1861 tggcttcatg caccacgtgc tggagaagag cgagttcagg gatgagtccc agtacttccg
     1921 ctttcatgct gacgaggaga tggaggggac cagcagcaag aacaaacagc ttcgcaacga
     1981 cttcaagctg gtggagaaca ttctggccaa gcgcctgctg atcctgcccc aggaggagga
     2041 ctatggcttt gacatcgagg agaagaacaa ggctgtggtg gtgaagtccg tccagagggg
     2101 ctcgctggct gaggtggctg gcctgcaggt ggggaggaag atctactcca tcaatgagga
     2161 cctggtgttc ctgcggccgt tttcagaggt ggagtccatc ctcaaccagt ccttctgctc
     2221 ccgccgccct ctgcgcctcc tggtggccac gaaggccaaa gagatcatca aaatccccga
     2281 ccagccggac acactgtgct tccagattcg tggagctgcc ccaccgtacg tctatgctgt
     2341 ggggagaggc tctgaggcca tggctgcagg gctctgtgct ggtcagtgca ttctgaaggt
     2401 caatggcagc aacgtgatga acgatggtgc ccctgaggtc ctggagcact tccaggcatt
     2461 ccggagtcgg cgcgaagagg ccctgggcct gtaccagtgg atctaccaca cccatgagga
     2521 tgcccaggaa gcacgagcca gtcaggaggc ctccactgag gaccccagtg gcgagcaggc
     2581 ccaggaggaa gaccaggctg attcagcctt cccactgctg tccctgggtc cccggctgag
     2641 cctgtgtgag gacagcccca tggtcaccct gactgtggac aacgtgcacc tggaacacgg
     2701 cgtggtgtat gagtatgtga gcacggcagg cgtcaggtgc catgtgctgg agaagatcgt
     2761 ggagccccgc ggctgcttcg gcctcaccgc caagatcctc gaggcctttg ctgccaatga
     2821 cagcgtcttc gtggagaact gcaggcggct catggccctg agcagcgcca tcgtgaccat
     2881 gccccacttt gagttccgca acatctgtga caccaagctg gagagcattg gccagaggat
     2941 tgcctgctac caggagtttg cagcccaact gaagagcagg gtcagcccac ccttcaaaca
     3001 agcccccctg gagccccacc cgctgtgtgg cctggacttc tgccccacca attgccacat
     3061 caacctcatg gaagtgtcct accccaagac caccccctca gtgggcaggt ccttcagcat
     3121 ccgctttgga cgcaaaccct ccctcatcgg ccttgacccg gagcaaggcc acctgaaccc
     3181 catgtcgtac acccagcact gcatcaccac catggctgct ccctcctgga agtgcttgcc
     3241 tgctgcagag ggtgatcccc aaggccaggg tctccatgat ggcagcttcg ggccagccag
     3301 tgggaccctt ggtcaggaag accggggcct cagcttccta ctcaagcagg aggaccgtga
     3361 gatccaggat gcctacctgc agctcttcac caagctggat gtggccctga aggagatgaa
     3421 gcaatatgtc acccagatca acaggctgct gtccaccatc acagagccca cctcgggtgg
     3481 gtcctgcgac gcatccttgg ctgaggaggc ctcctccctg cccctggtca gtgaagagag
     3541 cgagatggac aggagtgacc atgggggcat caagaaggtg tgcttcaagg tggccgagga
     3601 ggaccaggag gactcaggcc acgacaccat gagttatcgc gactcctaca gcgagtgtaa
     3661 cagcaatcga gactcggtcc tgtcctacac cagcgtgaga agtaacagct cctacttggg
     3721 cagcgacgag atggggtctg gagatgagct gccctgtgac atgcggatcc catctgacaa
     3781 gcaggacaag cttcatggct gcctggagca cctctttaac caggtggact ccatcaatgc
     3841 tctcctcaag gggccagtca tgagccgggc tttcgaagag accaagcatt tccctatgaa
     3901 ccacagctta caagagttta aacagaaaga agagtgtaca atccgtggcc ggagcctgat
     3961 ccagattagc atccaggagg acccctggaa cctccccaac tccatcaaga ccctggtgga
     4021 caacattcag agatatgtgg aagatgggaa gaaccagctg ctcctggcct tgctgaagtg
     4081 cacagacacg gagctgcagc tgcgcagaga cgcgatcttc tgccaggccc tggtggccgc
     4141 cgtgtgcacc ttctccaagc agctgctggc ggccctgggc taccgctaca acaacaatgg
     4201 cgagtacgag gagagcagcc gcgacgccag ccgcaagtgg ctggagcagg tggcggccac
     4261 gggcgtcctg ctgcactgcc agtccctgct ctcgccagcc acagtgaagg aggaacggac
     4321 catgctggag gacatctggg tgacgctgtc agagctggac aatgtcacct tctcctttaa
     4381 gcagctggac gagaactatg tggccaacac caacgtcttc taccacattg agggcagccg
     4441 gcaggcgctg aaggtcatct tctacctcga cagctaccac ttctccaagc tgccctcccg
     4501 cctggagggt ggggccagcc tgaggctgca cacagcgctg ttcacgaaag tgctggagaa
     4561 cgtggagggg ctgccttctc caggcagcca ggccgcggag gatttgcagc aggacatcaa
     4621 cgcgcagtcc ctggagaaag ttcagcagta ttaccgcaaa ctcagggcat tttacctgga
     4681 gcggtctaac ctgcccacgg atgccagcac cacggcggta aagatagacc agctgatccg
     4741 ccccatcaat gccctggatg agctctgccg cctcatgaag tcctttgtcc acccaaagcc
     4801 tggtgctgct gggagtgtgg gcgccggcct catccccatc tcctcggagc tctgctaccg
     4861 cctgggggcc tgccagatgg tcatgtgtgg cacaggcatg cagaggagca ccctgagcgt
     4921 gtccctggag caggcggcca tcttggcacg gagccacggg ttgctgccca agtgcatcat
     4981 gcaggccacg gacatcatgc ggaagcaggg cccaagggtg gagattctgg ccaaaaacct
     5041 gcgagtcaag gaccagatgc cccagggtgc tccgcgcctc taccgcctct gccagccgcc
     5101 ggtggatggg gacctctgaa cacccaaatg ccccacgctg ggccgcggcc tctggagctg
     5161 ggatttggga ggacacagca ggcagcgctg gccttctcca gggatggccc aaggcttccg
     5221 cagccgcccg ttccgggacc tgcccagcgt cctccctgcc tccttccggg acaagcctgg
     5281 ccaccctcgc tgtgatgacg agctggctga ttggccctgg gccggcccat tcttcacacg
     5341 cctgccagaa gctggagggg tgctggagac ccatagagct gatgggagca gctggtgcct
     5401 ggccttcggc tcctgcgtcc ccagaaccca agggaacgtc atggaggcca catggggcca
     5461 cccggctccc tcgggatggc tccgcctgca cttttgaaac cccggtttcc ttcaacgtcc
     5521 acattccagg tgaccacacg tgtctcctcc tcctcatctt agcttccagg ttcaccctaa
     5581 ccctgtacta acctgcttgg tggacttgga aaagacttgg ctctgtcggg aaaggagaga
     5641 cggggcctcc atcacgcctg ttaccagagg atccccgaga gccacaccag ctctggacat
     5701 caccgcccct ggaactgggg ccaccagccc tgggcacgag atttgctctg actttattta
     5761 tatggcatga aatctctggt ttattttggg attttttgtt gttggtgttg tcaaagtttg
     5821 ttttttctaa agttgtgtga ttatatattt gacattttac atttcaaaga aaggtatgtt
     5881 gtctaacagg ggaccaacag aaggtagtat tgacaactgt tcctgcttct actaaaaaaa
     5941 aaagagcaca aaagaaaaac taaattattg aaaaattaaa aaatgtcatt gtttcctgtt
     6001 tgttaatatt agggttgtaa ggtgtcgttt tgaggtatcg actgtgattc cttcccccac
     6061 cctccattct ccagcggttg gccggtgtta gaactcgctc tctttgagtg actggctaca
     6121 agggcctgag aggtggccag ccagggttgg agctggaggg gatggagccc cacctgaggt
     6181 gccgtgtcac acgggttaga gggtcactgg gaaacaccgg gcggtggctt ctgtgattta
     6241 ttttcttgat ggtaacttct cagagcaggg cgattgggac atcaccagcc agagcacagg
     6301 aagccaccct gcctgctggg gaggagggac ccacacaagc cccctcggca gtttgtcccc
     6361 ccagcttcgg tatgccttca gggaaaggtc acagctgggg aggaagcggg gggacgcctg
     6421 tcacccctgg caggtggtga gttcaggtgg gggctccctg ctgcccccag gcctgggagc
     6481 ttgaagccct cccggcatct ggcatccgag cctcccgccc tccagggtgc gcttccctct
     6541 cttgccgcag catacacgag ggcaggcagt ggccttgtca ctgtatcttg catcagagac
     6601 aaaggaggac ccgctttagc cctgctgcgg gaaatggggg agggcccagg gccagcgcat
     6661 tgtgcactgg tttactttaa aatgtacaga ttcttctcgt taaattcttg atagattttt
     6721 tattattatt aaaagtcagt ttataataca aa
//
