LOCUS       NM_013388               2127 bp    mRNA    linear   PRI 16-JUN-2024
DEFINITION  Homo sapiens prolactin regulatory element binding (PREB),
            transcript variant 1, mRNA.
ACCESSION   NM_013388
VERSION     NM_013388.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Melville,D.B., Studer,S. and Schekman,R.
  TITLE     Small sequence variations between two mammalian paralogs of the
            small GTPase SAR1 underlie functional differences in coat protein
            complex II assembly
  JOURNAL   J Biol Chem 295 (25), 8401-8412 (2020)
   PUBMED   32358066
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Yuan,L., Kenny,S.J., Hemmati,J., Xu,K. and Schekman,R.
  TITLE     TANGO1 and SEC12 are copackaged with procollagen I to facilitate
            the generation of large COPII carriers
  JOURNAL   Proc Natl Acad Sci U S A 115 (52), E12255-E12264 (2018)
   PUBMED   30545919
  REMARK    GeneRIF: Both cTAGE5 and SEC12 were exported with TANGO1 in large
            COPII carriers.
REFERENCE   3  (bases 1 to 2127)
  AUTHORS   Park,J.M., Kim,M.Y., Kim,T.H., Min,D.K., Yang,G.E. and Ahn,Y.H.
  TITLE     Prolactin regulatory element-binding (PREB) protein regulates
            hepatic glucose homeostasis
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1864 (6 Pt A), 2097-2107 (2018)
   PUBMED   29601978
  REMARK    GeneRIF: Administration of recombinant adenovirus containing human
            PREB to db/db, ob/ob, and DIO mice diminished glucose, insulin, and
            pyruvate tolerance indicating PREB to be a negative regulator of
            liver gluconeogenic genes.
REFERENCE   4  (bases 1 to 2127)
  AUTHORS   Sun,J., Yu,S., Zhang,X., Capac,C., Aligbe,O., Daudelin,T.,
            Bonder,E.M. and Gao,N.
  TITLE     A Wntless-SEC12 complex on the ER membrane regulates early Wnt
            secretory vesicle assembly and mature ligand export
  JOURNAL   J Cell Sci 130 (13), 2159-2171 (2017)
   PUBMED   28515233
  REMARK    GeneRIF: Wls-SEC12 binding is stable, with the interacting
            interface biochemically mapped to cytosolic segments of individual
            proteins. Mutant Wls that fails to communicate with the COPII
            machinery cannot effectively support Wnt secretion. These data
            suggest that formation of early Wnt secretory vesicles is carefully
            regulated to ensure proper export of functional ligands
REFERENCE   5  (bases 1 to 2127)
  AUTHORS   Maeda,M., Katada,T. and Saito,K.
  TITLE     TANGO1 recruits Sec16 to coordinately organize ER exit sites for
            efficient secretion
  JOURNAL   J Cell Biol 216 (6), 1731-1743 (2017)
   PUBMED   28442536
REFERENCE   6  (bases 1 to 2127)
  AUTHORS   Edgar,A.J.
  TITLE     The gene structure and expression of human ABHD1: overlapping
            polyadenylation signal sequence with Sec12
  JOURNAL   BMC Genomics 4 (1), 18 (2003)
   PUBMED   12735795
REFERENCE   7  (bases 1 to 2127)
  AUTHORS   Kirchhausen,T.
  TITLE     Three ways to make a vesicle
  JOURNAL   Nat Rev Mol Cell Biol 1 (3), 187-198 (2000)
   PUBMED   11252894
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol 2001 Mar;2(3):216]
REFERENCE   8  (bases 1 to 2127)
  AUTHORS   Taylor Clelland,C.L., Levy,B., McKie,J.M., Duncan,A.M.,
            Hirschhorn,K. and Bancroft,C.
  TITLE     Cloning and characterization of human PREB; a gene that maps to a
            genomic region associated with trisomy 2p syndrome
  JOURNAL   Mamm Genome 11 (8), 675-681 (2000)
   PUBMED   10920239
REFERENCE   9  (bases 1 to 2127)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   10 (bases 1 to 2127)
  AUTHORS   Fliss,M.S., Hinkle,P.M. and Bancroft,C.
  TITLE     Expression cloning and characterization of PREB (prolactin
            regulatory element binding), a novel WD motif DNA-binding protein
            with a capacity to regulate prolactin promoter activity
  JOURNAL   Mol Endocrinol 13 (4), 644-657 (1999)
   PUBMED   10194769
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC013403.9.
            
            On Nov 23, 2018 this sequence version replaced NM_013388.5.
            
            Summary: This gene encodes a protein that specifically binds to a
            Pit1-binding element of the prolactin (PRL) promoter. This protein
            may act as a transcriptional regulator and is thought to be
            involved in some of the developmental abnormalities observed in
            patients with partial trisomy 2p. This gene overlaps the
            abhydrolase domain containing 1 (ABHD1) gene on the opposite
            strand. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.691035.1,
                                           SRR3476690.597616.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260643.7/ ENSP00000260643.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-350               AC013403.9         74987-75336         c
            351-540             AC013403.9         74232-74421         c
            541-761             AC013403.9         73817-74037         c
            762-842             AC013403.9         73543-73623         c
            843-967             AC013403.9         73303-73427         c
            968-1141            AC013403.9         72930-73103         c
            1142-1214           AC013403.9         72710-72782         c
            1215-1374           AC013403.9         72372-72531         c
            1375-2127           AC013403.9         71456-72208         c
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..2127
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /note="prolactin regulatory element binding"
                     /db_xref="GeneID:10113"
                     /db_xref="HGNC:HGNC:9356"
                     /db_xref="MIM:606395"
     exon            1..350
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1469
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prolactin regulatory element-binding protein; mammalian
                     guanine nucleotide exchange factor mSec12; guanine
                     nucleotide-exchange factor SEC12"
                     /codon_start=1
                     /product="guanine nucleotide-exchange factor SEC12 isoform
                     1"
                     /protein_id="NP_037520.1"
                     /db_xref="CCDS:CCDS1738.1"
                     /db_xref="GeneID:10113"
                     /db_xref="HGNC:HGNC:9356"
                     /db_xref="MIM:606395"
                     /translation="MGRRRAPELYRAPFPLYALQVDPSTGLLIAAGGGGAAKTGIKNG
                     VHFLQLELINGRLSASLLHSHDTETRATMNLALAGDILAAGQDAHCQLLRFQAHQQQG
                     NKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELRVENLQAVQTDFSSDPLQKVV
                     CFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIEDLALGPDGKLVTVGRDLK
                     ASVWQKDQLVTQLHWQENGPTFSSTPYRYQACRFGQVPDQPAGLRLFTVQIPHKRLRQ
                     PPPCYLTAWDGSNFLPLRTKSCGHEVVSCLDVSESGTFLGLGTVTGSVAIYIAFSLQC
                     LYYVREAHGIVVTDVAFLPEKGRGPELLGSHETALFSVAVDSRCQLHLLPSRRSVPVW
                     LLLLLCVGLIIVTILLLQSAFPGFL"
     exon            351..540
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            541..761
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            762..842
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            843..967
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1141
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1214
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1374
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..2127
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2098..2103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /note="hexamer: AACAAG"
     polyA_site      2127
                     /gene="PREB"
                     /gene_synonym="SEC12"
                     /note="major polyA site"
ORIGIN      
        1 agtgcgcgtc tgcgagaccg acttggacgg agccgagctg aggctcggct tcctgctgat
       61 ggtcagggtt ttggcaactc cccggtgtga gaggggtagg gagtgctccc ggcggcgacg
      121 gggccgagtt caccagccgc cggggcagta gtcgaaggcc cggcgcggca tgtcctgggt
      181 gccgcggtgc gggcagtgaa cgcgcgccgg gcgggatggg ccggcgccgg gcgccagagc
      241 tgtaccgggc tccgttcccg ttgtacgcgc ttcaggtcga ccccagcact gggctgctca
      301 tcgctgcggg cggaggaggc gccgccaaga caggcataaa gaatggcgtg cactttctgc
      361 agctagagct gattaatggg cgcttgagtg cctccttgct gcactcccat gacacagaga
      421 cacgggccac catgaacttg gcactggctg gtgacatcct tgctgcaggg caggatgccc
      481 actgtcagct cctgcgcttc caggcacatc aacagcaggg caacaaggca gagaaggccg
      541 gttccaagga gcaggggcct cgacaaagga agggagcagc cccagcagag aagaaatgtg
      601 gagcggaaac ccagcacgag gggctagaac tcagggtaga gaatttgcag gcggtgcaga
      661 cagactttag ctccgatcca ctgcagaaag ttgtgtgctt caaccacgat aataccctgc
      721 ttgccactgg aggaacagat ggctacgtcc gtgtctggaa ggtgcccagc ctggagaagg
      781 ttctggagtt caaagcccac gaaggggaga ttgaagacct ggctttaggg cctgatggca
      841 agttggtaac cgtgggccgg gaccttaagg cctctgtgtg gcagaaggat cagctggtga
      901 cacagctgca ctggcaagaa aatggaccca ccttttccag cacaccttac cgctaccagg
      961 cctgcaggtt tgggcaggtt ccagaccagc ctgctggcct gcgactcttc acagtgcaaa
     1021 ttccccacaa gcgcctgcgc cagccccctc cctgctacct cacagcctgg gatggctcca
     1081 acttcttgcc ccttcggacc aagtcctgtg gccatgaagt cgtctcctgc ctcgatgtca
     1141 gtgaatccgg caccttccta ggcctgggca cagtcactgg ctctgttgcc atctacatag
     1201 ctttctctct ccagtgcctc tactacgtga gggaggccca tggcattgtg gtgacggatg
     1261 tggcctttct acctgagaag ggtcgtggtc cagagctcct tgggtcccat gaaactgccc
     1321 tgttctctgt ggctgtggac agtcgttgcc agctgcatct gttgccctca cggcggagtg
     1381 ttcctgtgtg gctcctgctc ctgctgtgtg tcgggcttat tattgtgacc atcctgctgc
     1441 tccagagtgc ctttccaggt ttcctttagc ttccctgctt cctgggaatc aggagcctgg
     1501 acactgccat ctctagagca gagtggaggc ctggactccc tttgctcact ccattcgggt
     1561 ccacagctga ggttgccgct gacaagatga atgggcactg cctgcccttc tagtgaaaag
     1621 gcttggctat ggccctgtgt gactccaggt cccaggaacc ttgccttcgt catctgtgga
     1681 tccatccaga acagcggtat ctgaagccca ggccatactc cctgcctcct ttcttctgcc
     1741 taccagaggc tccagagttg agcttgtcct tatctagaaa catgtgaaga tgcccaagag
     1801 cctggaggca ctgctgtcct tcctgcagaa acagtttctc ctcctcccct cagccttgtg
     1861 gccagttcct cttcacatga agcccctggc atttgctggg gaagggactg gcctggtact
     1921 tgctgttagg gcaggaaggg gcaaaaggaa gacttgggta gtaatctggg ggttcagatg
     1981 ggtagcacta agccagctgg cctaaagatg caataagttc ctaggtagtc tacccttacc
     2041 ttgaggaatg ggaaaatgaa cctcagccca ttaggcagga aaagttgata tttaataaac
     2101 aaggaaagag tgaactgaga ccccaaa
//
