LOCUS       NM_001136239            9147 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens PR/SET domain 6 (PRDM6), mRNA.
ACCESSION   NM_001136239 XM_001723255 XM_927647 XM_937753
VERSION     NM_001136239.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Schmidt,C., Cohen,S., Gudenas,B.L., Husain,S., Carlson,A.,
            Westelman,S., Wang,L., Phillips,J.J., Northcott,P.A., Weiss,W.A.
            and Schwer,B.
  TITLE     PRDM6 promotes medulloblastoma by repressing chromatin
            accessibility and altering gene expression
  JOURNAL   Sci Rep 14 (1), 16074 (2024)
   PUBMED   38992221
  REMARK    GeneRIF: PRDM6 promotes medulloblastoma by repressing chromatin
            accessibility and altering gene expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Stanley,H.M., White,B.R., LaRosa,C.J., Cocalis,M.W., Gaynor,J.W.,
            Strong,A. and Gangaram,B.
  TITLE     Patent ductus arteriosus and coarctation of the aorta in
            association with PRDM6 variants
  JOURNAL   Am J Med Genet A 194 (4), e63500 (2024)
   PUBMED   38071433
  REMARK    GeneRIF: Patent ductus arteriosus and coarctation of the aorta in
            association with PRDM6 variants.
REFERENCE   3  (bases 1 to 9147)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9147)
  AUTHORS   Riveros-McKay,F., Mistry,V., Bounds,R., Hendricks,A., Keogh,J.M.,
            Thomas,H., Henning,E., Corbin,L.J., O'Rahilly,S., Zeggini,E.,
            Wheeler,E., Barroso,I. and Farooqi,I.S.
  CONSRTM   Understanding Society Scientific Group
  TITLE     Genetic architecture of human thinness compared to severe obesity
  JOURNAL   PLoS Genet 15 (1), e1007603 (2019)
   PUBMED   30677029
  REMARK    GeneRIF: we detected a novel obesity and BMI-associated locus at
            PKHD1 and novel variants driving associations at previously
            established signals (e.g. rs205262 at the SNRPC/C6orf106 locus and
            rs112446794 at the PRDM6-CEP120 locus).
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9147)
  AUTHORS   Li,N., Subrahmanyan,L., Smith,E., Yu,X., Zaidi,S., Choi,M.,
            Mane,S., Nelson-Williams,C., Behjati,M., Kazemi,M., Hashemi,M.,
            Fathzadeh,M., Narayanan,A., Tian,L., Montazeri,F., Mani,M.,
            Begleiter,M.L., Coon,B.G., Lynch,H.T., Olson,E.N., Zhao,H.,
            Ruland,J., Lifton,R.P. and Mani,A.
  TITLE     Mutations in the Histone Modifier PRDM6 Are Associated with
            Isolated Nonsyndromic Patent Ductus Arteriosus
  JOURNAL   Am J Hum Genet 99 (4), 1000 (2016)
   PUBMED   27716515
  REMARK    Correction to:[Am J Hum Genet. 2016 Jun 2;98(6):1082-1091. doi:
            10.1016/j.ajhg.2016.03.022.. PMID: 27181681]
REFERENCE   6  (bases 1 to 9147)
  AUTHORS   Li,N., Subrahmanyan,L., Smith,E., Yu,X., Zaidi,S., Choi,M.,
            Mane,S., Nelson-Williams,C., Behjati,M., Kazemi,M., Hashemi,M.,
            Fathzadeh,M., Narayanan,A., Tian,L., Montazeri,F., Mani,M.,
            Begleiter,M.L., Coon,B.G., Lynch,H.T., Olson,E.N., Zhao,H.,
            Ruland,J., Lifton,R.P. and Mani,A.
  TITLE     Mutations in the Histone Modifier PRDM6 Are Associated with
            Isolated Nonsyndromic Patent Ductus Arteriosus
  JOURNAL   Am J Hum Genet 98 (6), 1082-1091 (2016)
   PUBMED   27181681
  REMARK    GeneRIF: Mutations in the Histone Modifier PRDM6 Are Associated
            with Isolated Nonsyndromic Patent Ductus Arteriosus.
            Erratum:[Am J Hum Genet. 2016 Oct 6;99(4):1000. doi:
            10.1016/j.ajhg.2016.09.003.. PMID: 27716515]
REFERENCE   7  (bases 1 to 9147)
  AUTHORS   Hong,K.W., Lim,J.E., Kim,J.W., Tabara,Y., Ueshima,H., Miki,T.,
            Matsuda,F., Cho,Y.S., Kim,Y. and Oh,B.
  TITLE     Identification of three novel genetic variations associated with
            electrocardiographic traits (QRS duration and PR interval) in East
            Asians
  JOURNAL   Hum Mol Genet 23 (24), 6659-6667 (2014)
   PUBMED   25035420
  REMARK    GeneRIF: Three novel loci were identified in East Asians with
            cardiac arrhythmias: rs2483280 (PRDM16 locus) and rs335206 (PRDM6
            locus) were associated with QRS duration; and rs17026156 (SLC8A1
            locus) correlated with PR interval.
REFERENCE   8  (bases 1 to 9147)
  AUTHORS   Lindstrom,S., Thompson,D.J., Paterson,A.D., Li,J., Gierach,G.L.,
            Scott,C., Stone,J., Douglas,J.A., dos-Santos-Silva,I.,
            Fernandez-Navarro,P., Verghase,J., Smith,P., Brown,J., Luben,R.,
            Wareham,N.J., Loos,R.J., Heit,J.A., Pankratz,V.S., Norman,A.,
            Goode,E.L., Cunningham,J.M., deAndrade,M., Vierkant,R.A., Czene,K.,
            Fasching,P.A., Baglietto,L., Southey,M.C., Giles,G.G., Shah,K.P.,
            Chan,H.P., Helvie,M.A., Beck,A.H., Knoblauch,N.W., Hazra,A.,
            Hunter,D.J., Kraft,P., Pollan,M., Figueroa,J.D., Couch,F.J.,
            Hopper,J.L., Hall,P., Easton,D.F., Boyd,N.F., Vachon,C.M. and
            Tamimi,R.M.
  TITLE     Genome-wide association study identifies multiple loci associated
            with both mammographic density and breast cancer risk
  JOURNAL   Nat Commun 5, 5303 (2014)
   PUBMED   25342443
  REMARK    Erratum:[Nat Commun. 2015 Sep 09;6:8358. doi: 10.1038/ncomms9358..
            PMID: 26349435]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 9147)
  AUTHORS   Davis,C.A., Haberland,M., Arnold,M.A., Sutherland,L.B.,
            McDonald,O.G., Richardson,J.A., Childs,G., Harris,S., Owens,G.K.
            and Olson,E.N.
  TITLE     PRISM/PRDM6, a transcriptional repressor that promotes the
            proliferative gene program in smooth muscle cells
  JOURNAL   Mol Cell Biol 26 (7), 2626-2636 (2006)
   PUBMED   16537907
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC106786.2 and AC008548.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001136239.3.
            
            Summary: The protein encoded by this gene is a transcriptional
            repressor and a member of the PRDM family. Family members contain a
            PR domain and multiple zinc-finger domains. The encoded protein is
            involved in regulation of vascular smooth muscle cells (VSMC)
            contractile proteins. Mutations in this gene result in patent
            ductus arteriosus 3 (PDA3). [provided by RefSeq, Apr 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968540,
                              SAMEA2142853 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000407847.5/ ENSP00000384725.3
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-279               AC106786.2         66544-66822
            280-886             AC106786.2         67303-67909
            887-1194            AC106786.2         76957-77264
            1195-1322           AC008548.6         72306-72433
            1323-1447           AC008548.6         75936-76060
            1448-1790           AC008548.6         87188-87530
            1791-1967           AC008548.6         96569-96745
            1968-9147           AC008548.6         103509-110688
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q23.2"
     gene            1..9147
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /note="PR/SET domain 6"
                     /db_xref="GeneID:93166"
                     /db_xref="HGNC:HGNC:9350"
                     /db_xref="MIM:616982"
     exon            1..279
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            280..886
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..2082
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /EC_number="2.1.1.361"
                     /note="PR domain 6; PR-domain zinc finger protein 6; PR
                     domain-containing protein 6; PR domain containing 6"
                     /codon_start=1
                     /product="putative histone-lysine N-methyltransferase
                     PRDM6"
                     /protein_id="NP_001129711.1"
                     /db_xref="CCDS:CCDS47259.1"
                     /db_xref="GeneID:93166"
                     /db_xref="HGNC:HGNC:9350"
                     /db_xref="MIM:616982"
                     /translation="MLKPGDPGGSAFLKVDPAYLQHWQQLFPHGGAGPLKGSGAAGLL
                     SAPQPLQPPPPPPPPERAEPPPDSLRPRPASLSSASSTPASSSTSASSASSCAAAAAA
                     AALAGLSALPVSQLPVFAPLAAAAVAAEPLPPKELCLGATSGPGPVKCGGGGGGGGEG
                     RGAPRFRCSAEELDYYLYGQQRMEIIPLNQHTSDPNNRCDMCADNRNGECPMHGPLHS
                     LRRLVGTSSAAAAAPPPELPEWLRDLPREVCLCTSTVPGLAYGICAAQRIQQGTWIGP
                     FQGVLLPPEKVQAGAVRNTQHLWEIYDQDGTLQHFIDGGEPSKSSWMRYIRCARHCGE
                     QNLTVVQYRSNIFYRACIDIPRGTELLVWYNDSYTSFFGIPLQCIAQDENLNVPSTVM
                     EAMCRQDALQPFNKSSKLAPTTQQRSVVFPQTPCSRNFSLLDKSGPIESGFNQINVKN
                     QRVLASPTSTSQLHSEFSDWHLWKCGQCFKTFTQRILLQMHVCTQNPDRPYQCGHCSQ
                     SFSQPSELRNHVVTHSSDRPFKCGYCGRAFAGATTLNNHIRTHTGEKPFKCERCERSF
                     TQATQLSRHQRMPNECKPITESPESIEVD"
     exon            887..1194
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1322
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1447
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1790
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1967
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..9147
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2906..2911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /note="hexamer: AATAAA"
     polyA_site      2932
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /note="major polyA site"
     regulatory      9122..9127
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
                     /note="hexamer: AATAAA"
     polyA_site      9147
                     /gene="PRDM6"
                     /gene_synonym="KMT8C; PDA3; PRISM"
ORIGIN      
        1 gccagagcgt ggaaccaagg agccaggacg cggcagcggc caagcgcagc agcccacggc
       61 ggttgagtcg ggcgcccagg tccgtccgca ctctcgcgcc ctccgcgggc ctcccaattt
      121 tctcgcttgc aggtcgggag gtttccgggc ggcacaatct ctaggactct cctcccgcgc
      181 tgctcagggg catgtagcgc acgcagggcg cacactctcg cgcacccgca cgctcaccga
      241 gacacccgca cgcacccacc ggcagcaccg agttttcagt tcgaggcgcc ggacatgctg
      301 aagcccggag accccggcgg ttcggccttc ctcaaagtgg acccagccta cctgcagcac
      361 tggcagcaac tcttccctca cggaggcgca ggcccgctca agggcagcgg cgccgcgggt
      421 ctcctgagcg cgccgcagcc tcttcagccg ccgccgccgc ccccgccccc ggagcgcgct
      481 gagcctccgc cggacagcct gcgcccgcgg cccgcctctc tctcctccgc ctcgtccacg
      541 ccggcttcct cttccacctc cgcctcctcc gcctcctcct gcgctgctgc ggccgctgcc
      601 gccgcgctgg ctggtctctc ggccctgccg gtgtcgcagc tgccggtgtt cgcgcctcta
      661 gccgccgctg ccgtcgccgc cgagccgctg ccccccaagg aactgtgcct cggcgccacc
      721 tccggccccg ggcccgtcaa gtgcggtggt ggtggcggcg gcggcgggga gggtcgcggc
      781 gccccgcgct tccgctgcag cgcagaggag ctggactatt acctgtatgg ccagcagcgc
      841 atggagatca tcccgctcaa ccagcacacc agcgacccca acaaccgttg cgacatgtgc
      901 gcggacaacc gcaacggcga gtgccctatg catgggccac tgcactcgct gcgccggctt
      961 gtgggcacca gcagcgctgc ggccgccgcg cccccgccgg agctgccgga gtggctgcgg
     1021 gacctgcctc gcgaggtgtg cctctgcacc agtactgtgc ccggcctggc ctacggcatc
     1081 tgcgcggcgc agaggatcca gcaaggcacc tggattggac ctttccaagg cgtgcttctg
     1141 cccccagaga aggtgcaggc aggcgccgtg aggaacacgc agcatctctg ggagatatat
     1201 gaccaggatg ggacactaca gcactttatt gatggtgggg aacctagtaa gtcgagctgg
     1261 atgaggtata tccgatgtgc aaggcactgc ggagaacaga atctaacagt agttcagtac
     1321 aggtcgaata tattctaccg agcctgtata gatatcccta ggggcaccga gcttctggtg
     1381 tggtacaatg acagctatac gtctttcttt gggatcccct tacaatgcat tgcccaggat
     1441 gaaaacttaa atgtcccttc aacggtaatg gaagccatgt gcagacaaga cgccctgcag
     1501 cccttcaaca aaagcagcaa actcgcccct accacccagc agcgctccgt tgttttcccc
     1561 cagactccgt gcagcaggaa cttctctctt ctggataagt ctgggcccat tgaatcagga
     1621 tttaatcaaa tcaacgtgaa aaaccagcga gtcctggcaa gcccaacttc cacaagccag
     1681 ctccactcgg agttcagtga ctggcatctt tggaaatgtg ggcagtgctt taagactttc
     1741 acccagcgga tcctcttaca gatgcacgtg tgcacgcaga accccgacag accctaccaa
     1801 tgcggccact gctcccagtc cttttcccag ccttcagaac tgaggaacca cgtggtcact
     1861 cactctagtg accggccttt caagtgcggc tactgtggtc gtgcctttgc cggggccacc
     1921 accctcaaca accacatccg aacccacact ggagaaaagc ccttcaagtg cgagaggtgt
     1981 gagaggagct tcacgcaggc cacccagctg agccgacacc agcggatgcc caatgagtgc
     2041 aagccaataa ctgagagccc agaatcaatc gaagtggatt aacggattga ctggttggaa
     2101 ttaaactgca aggaaagtca tgattaaatg tcacggacac ttaagcaaaa ccaaagattt
     2161 cctctgagca actttcaatc agtcccagaa aaccaaaagc agtaataaaa taagtaagat
     2221 gttaagagat attgatcctg gcatggaagt cagaccagga aagagattat ttatttatga
     2281 cttagggatg agacttattt cagtggacaa ctaacctggg atggttaaca tttccagtcc
     2341 caccatgtat tttgctttgt ttctaaaaag ctttttaaaa actgttattt aataccaaag
     2401 ggaggaatcg tatgggttct tctgcccacc gttgtgacta agaatgcaca gggacttggt
     2461 tctcgttgca ccttttttta gtaacatgtt tcatggggac ccactgtaca gcccttcatt
     2521 ctgctgtgtc agtttggcct ggcctgacac tggctgcccc agcggggacc acggaagcag
     2581 agtgagagcc ttcgctgagt caatgctacc ttcagcccca gacgcatccc atttccatgt
     2641 cttccatgct cactgctcat gcacttttta cacggtttct tccaaacagc ccggtcttga
     2701 tgcaggagag tctggaaaag gaagaaaatg gtttcagttt caaaattcaa aggaaaaagt
     2761 tgaggactta ttttgtcctg tcaagattgc aagaacatgt aaaatgtacg gagcttcata
     2821 atacgttata ttgttccgaa gcagctcgtt gagaaacatt tgttttcaat aacattttag
     2881 cttaaaaaaa aaaaaagaaa atgaaaataa agttctttgg tttaaggctg gagataaggt
     2941 gttaggcttc attgtaattt ttttagttgt ttctgtgaaa gtgttgaaca tgtgaacaaa
     3001 aatagtgcca aaccccatcg tcaaggccct tttagcaatt ttatctttct tcctcttgca
     3061 gatgaacccc aggagccccc tttcctctct cctgagtgtg ggaggagcat gtctacaaac
     3121 acaagtgaaa atagcccaga caaggagact ttgagaggag gaaccatgga gaaggttgtg
     3181 gttagaacag atagcccagg agcagctaat tttatcaata ggattacttt gtcttttctt
     3241 gagattgatt aattgaaaga gaaggaattc tccacacaat ttcactattc tttgagtgct
     3301 gagaatgtga gtttaatttc agctagcctg ttggtggttc aacagtggtg ctcaatgaaa
     3361 ttgggtcaca tttaatgaaa tgatctagtt ttgggaagag ctgatagctc ccagcagcat
     3421 ctcagcgact gtctaccttg atggccagtt tagataaaaa ctgttcagtt ttatctagag
     3481 tgtttaataa caacagtttc tgtttgtaaa tttcatcatc acctgatatc aggacacagt
     3541 cttttaagga actccttgtc tttgagcttt cccattgtct tccctcaata aaaatgagac
     3601 acataactga actcactact aaaaggcaag gatactgtgt ggtttagttt aatctattat
     3661 tatgtatctg gctctgagaa aatctctctt cccttttaat tttgcttctc tattccttag
     3721 caccccagct accacatttt aaatggaatt caacagggtc taattaataa gtgtttacag
     3781 ttattattta tatacgtata tatacatata tacacataat aataatctct agaaccctgg
     3841 gccataaaga aaaaaatggc taattctgac ctctgttgcc caatctcaag ccattcacct
     3901 agaattttcc tccagttctt actatctcct ggtcatgaaa gtagtaagga atctatgctg
     3961 ctgtataaca gtagctgatt gaaactattc tctctggtag ttaggaatgt gttctgattt
     4021 tacaattccc tgccctaagt gatggatacg tttgcaaaca atgtgcgtca ctgggaagtg
     4081 tgaatgaaat gtagccatat taacctgaaa cctgcaagaa aaggacagat cagcttcaca
     4141 gatgagtaag ctgttccatt tgttaaggtt gaaagattta ctcaaacttt ttgaaaacaa
     4201 cagtagggaa tcacctgtat atttctaatt ttttttttca tttgaaaaat cagctcagcc
     4261 atttgccaca tttcacagtg gaacaccatg tactactcag ttctcatgag cttcagaacg
     4321 tgggagaatg agaaggcttc cctcctccct tgatgtctgg catgggaagg ttttccttaa
     4381 caatgatctc tctgccaccc tgatagcttt gctagctgat cccaaacttc cactgaatgt
     4441 ggcagtaacc tgtactccaa aacactcttc tagtgttctg ctccatttaa atctattttt
     4501 atcctctatt attaagagaa attgttggca tatatattcc accaaatttg gtcagaaaat
     4561 attttctgcc taacaaatag aaaattggat actctaattt tccaggcatg tgggcacatt
     4621 cacaattttt acattgctgt cctattgcca gcttctagca gaccagccta ggctgtctgt
     4681 ccacaaagac aggcttacac ctggacattg acttggaacc ctgaagcagg tactgagatc
     4741 atttgggtcc aaaatgattt taatctaagt gtatgctggg gaggaggttt ctctttgaaa
     4801 cttcctaaca attgctttag ccttttctat ttaaagtgga cacacagtcc cctgggatac
     4861 agcaaatggg tggtagaaat tgcagtcgta aaatttgcca gtttttctta ttagtaactt
     4921 cctaattagt agtgctataa agagagtaaa tgctctgaat cactggggaa aggatattta
     4981 agataagaaa ggtagtctgt cagagctgaa tcagggtttg gctcttgaag caatccttct
     5041 ttactctttt ctttcaatgg ggataaaata ataataattt tttcagaata agacttttca
     5101 tcctagcgta tgttccttta tttagcagag tctaagacga aaggtaatgc cagggaaaag
     5161 tcagggtgca ataggatgag agatggagct gcagaccttt gcatttctag aaaaatctca
     5221 atatcccaag gcaaagttgt attttcccca ggatatgtgc atacattttt catagataag
     5281 cttttcattt ttgttttgtt tttatcttta aaaatgactt ttttggagga gatatacttc
     5341 taaaaagtta ttgtatggct aattaaagct atgaaactta atctcatata atttatataa
     5401 tttgattcag ttggtacatt ttttattgtg ttcatttaaa tgtagccttc ttgggaaact
     5461 atgtataaca ggaaaagatc atgacatcat tgtgtcctca tagggctcat aattgaattt
     5521 taccttgtaa aaatttcatt ctggttcagg tatgcaaaga tttatacgaa catttagcag
     5581 ttcatcaatt tttaaatcag atgttcattg tttcattcat ttattcaaca agtatttatt
     5641 gaactcttat tttatataaa gaccccattt tatgtgttca ccataagcag cattatttaa
     5701 gtgactattc atgataaaat aatattctcc tcctaagtgg ctgctagtaa aatgggtatt
     5761 ttagtccttt agaccattgt ctcagtgaag ttaaagattt tgttccaact gggaaaatct
     5821 gagagaggta aattcatctg gggaccagaa aatagcatat tgcaaaacaa gttaagatgc
     5881 acaacttggt tgtctttgct taactcactg ggctattggt tgttagcagc agtatcaaga
     5941 gaccaggatt agggcctggg tcttgggttt ttatgacccc ccctttcaat gataaggcaa
     6001 caagttgggt acaaggctgg agttgctcag ggaaggagat ctgcgaccct gaatgggtca
     6061 gagttgcctc attagtggta agcgttggca gtgtagtccc tttccccggt gtgcagaagt
     6121 aacattattt gttttgcact aatttaaaat ttaaaatgca agtatctatt gagttgttta
     6181 aaatgggaat catggcacgt ctcacaaaga gggcatttcc agaacagcag ggttaacggg
     6241 tccctctgag tgtacccagc agcctgcgtt taggagcaaa ttcattgctc tcatattaag
     6301 gctatgcatg cctacagaaa ccaggccagg aataagaaat aaataatcac atttcctgaa
     6361 atgtttatct gtttcttcct tgacatgaat ttaattttta tttcccagag tctttactag
     6421 atcctgacat cagattttaa aagatactga gatatatgct tctttaaatg ataactgtaa
     6481 gttccagaaa tgttattcac ttgtcaggat ccttttaagg tgaagagctt tggaaaattt
     6541 tatgcgctaa ctagatacct gggtgatcat caacccaaag tttaaagttc tcccctcact
     6601 agcatttatc tgaactacca caaaacaaca acgcaggtta ctaggaaggc acctatttca
     6661 gccctcccca agattgcatt ctacaaaccc agagatcagc tgtcaggtac aactggccag
     6721 acagagccaa actgaaaaga atattgcttt atctgtggaa accttgccac agatgtttaa
     6781 cctgtagtcc atggctgtgt ttgacattta aaatatttat ttagtttctt cctttaaata
     6841 ataaatgcca gatgtttcat cagatcaata tgtaaaacga taacaaaaaa tatgtcggaa
     6901 acaggtcaaa gtcatgaaac atttgaattg tctttcctat agactgaata tgccaccact
     6961 gcacttagaa aacctatgga ggaaatttta atggaaaaat aaggacattc tgcgcttatt
     7021 cccacagagg ttgatgggaa tgatgatgat tataaattat attgtgcaat tactgccatg
     7081 ccttgttcta atgcttaatg tggattatct cttttaatcc ttagtaactc tataacaaag
     7141 atactatcat tatccccttc tcatatatgc ttaacagttt tagctttgta gcttctttag
     7201 tttttttcct ccttcaaagc cataaacagg caattgttaa aagaggaaaa tacttctcta
     7261 tgtaaagctt ttttttgaga acaagagctt ttaaaaatta ataaacacaa gtataattta
     7321 tttataaaat gtgtttgtgg aaacaatact gaagtgctat taaaacacgt atcattatag
     7381 atgctgattt tgtcatgccc aaatctcatt gctggacaga atttcaacta ttccattgct
     7441 gtcaggcatt gtttttgttt ttctaagcag gaatctaaaa ttccacattg aaagaaaaaa
     7501 aaataaccct ctcaagtgtg atatacatta ggacaaatgg caacaggcag gaagggcagg
     7561 ttgtgttgga gggtggccag aagagaataa ggttcgaaca ctatcgacag gtaaaaacac
     7621 atcctcaacg gggctaagtg aaatctcgtt ttctttcttt gttctgactg aatgctaagt
     7681 gagttacttt ccaggcatca atggagtcat tacgcagttg tgtgcattaa gaatatgggc
     7741 ttatgttgtt agcctgttgg tttaaagcag tgcagcagaa agactggagt cattataggt
     7801 ttggtttgtc aaaggcacag atagtgtagt tacatgcatg aggtactcaa ggaaccagtt
     7861 caagttctgg ggcacaatct agatgaaact gtgaggtttg ttcaaaaagt aagtgtacaa
     7921 aatttcatca tgaactggct ttattacaac tctttaccgt ggtttattga aaccacaatg
     7981 tatactactt tcctccagat tctgattcta atgggcacat ttgtttgttg aaatgataaa
     8041 tatattttcc ttgtcaaaca tggtgggtcc tggttgatat ctcctgtttc ctgccttgaa
     8101 aaacatcctt gttggtgcct ttctcccact ttggtacatc actcccaact gaaaacagtc
     8161 tagatataaa ctttaacaag ctggagatct atttctacag cacaactctt tcatgtttaa
     8221 aagcaattat gccgtatcag gtgattgcca catgttgggg aaagcagcca tcttagagca
     8281 aaagtctgcc aacccacagt ccctgttgat ccacatgtgg gcattgtcta atgttaacaa
     8341 aatcagcact tgtagactta ctttctctga acaatctagt acacttctct agtttctccc
     8401 aacctggaga aaatgtatag caaaagccag aggcaaaatt attctcctta gcagttctaa
     8461 gtatttcatg aaaaagcctt cccccagttt aaatgcagag gctaaggtga tgactaacga
     8521 attaaggaaa actttggctc agatcagcaa aattattttg gaaacttaat gtcccaagtt
     8581 aattattttt tttatatcaa ggggaagaca tccagttctg taggatgcta atagaagtgt
     8641 gatatttaga tgctattagg acattgtata catttcttta atgtggttat tttaagttta
     8701 ggactgtaaa tgttccctac tggaatagaa atatgtctct ctccaacttt ccaccaaata
     8761 aaatggttag agtgaatgcc cagtttcaca tctgcctttt ggagtttgga aagcttaaac
     8821 aagtcagtta agagaatgag aagaatctca ctgtgggtga taaggagtgt gtagtttgct
     8881 gtaagggcca ctgatatgac aagaatgatt atcccacagt aagaatgggt aaaacttaat
     8941 aaagttttct aactttatta aactcacatt atttaatgaa aaaataactg caaaggacca
     9001 atgagatcat gcatgctgct gtattcttgt gatggaatct accaggatta agttgtattt
     9061 aaatgaaaaa acaggtacac atttagagat cattgaccaa gaaatgtaaa tatatttgat
     9121 taataaaacg tttttatgat gactcga
//
