LOCUS       NM_022114               8698 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens PR/SET domain 16 (PRDM16), transcript variant 1, mRNA.
ACCESSION   NM_022114 XM_001126711
VERSION     NM_022114.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Mao,L., Lu,J., Hou,Y. and Nie,T.
  TITLE     Directly targeting PRDM16 in thermogenic adipose tissue to treat
            obesity and its related metabolic diseases
  JOURNAL   Front Endocrinol (Lausanne) 15, 1458848 (2024)
   PUBMED   39351529
  REMARK    GeneRIF: Directly targeting PRDM16 in thermogenic adipose tissue to
            treat obesity and its related metabolic diseases.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Bathina,S., Colleluori,G., Villareal,D.T., Aguirre,L., Chen,R. and
            Armamento-Villareal,R.
  TITLE     A PRDM16-driven signal regulates body composition in
            testosterone-treated hypogonadal men
  JOURNAL   Front Endocrinol (Lausanne) 15, 1426175 (2024)
   PUBMED   39286266
  REMARK    GeneRIF: A PRDM16-driven signal regulates body composition in
            testosterone-treated hypogonadal men.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8698)
  AUTHORS   Xu,F., Jiang,H., Li,X., Pan,J., Li,H., Wang,L., Zhang,P., Chen,J.,
            Qiu,S., Xie,Y., Li,Y., Zhang,D. and Dong,Z.
  TITLE     Discovery of PRDM16-Mediated TRPA1 Induction as the Mechanism for
            Low Tubulo-Interstitial Fibrosis in Diabetic Kidney Disease
  JOURNAL   Adv Sci (Weinh) 11 (7), e2306704 (2024)
   PUBMED   38072665
  REMARK    GeneRIF: Discovery of PRDM16-Mediated TRPA1 Induction as the
            Mechanism for Low Tubulo-Interstitial Fibrosis in Diabetic Kidney
            Disease.
REFERENCE   4  (bases 1 to 8698)
  AUTHORS   Sun,B., Rouzbehani,O.M.T., Kramer,R.J., Ghosh,R., Perelli,R.M.,
            Atkins,S., Fatahian,A.N., Davis,K., Szulik,M.W., Goodman,M.A.,
            Hathaway,M.A., Chi,E., Word,T.A., Tunuguntla,H., Denfield,S.W.,
            Wehrens,X.H.T., Whitehead,K.J., Abdelnasser,H.Y., Warren,J.S.,
            Wu,M., Franklin,S., Boudina,S. and Landstrom,A.P.
  TITLE     Nonsense Variant PRDM16-Q187X Causes Impaired Myocardial
            Development and TGF-beta Signaling Resulting in Noncompaction
            Cardiomyopathy in Humans and Mice
  JOURNAL   Circ Heart Fail 16 (12), e010351 (2023)
   PUBMED   38113297
  REMARK    GeneRIF: Nonsense Variant PRDM16-Q187X Causes Impaired Myocardial
            Development and TGF-beta Signaling Resulting in Noncompaction
            Cardiomyopathy in Humans and Mice.
REFERENCE   5  (bases 1 to 8698)
  AUTHORS   Yan,C., Wang,P., Zhao,C., Yin,G., Meng,X., Li,L., Cai,S. and
            Meng,B.
  TITLE     Long Noncoding RNA MAGI2-AS3 Represses Cell Progression in Clear
            Cell Renal Cell Carcinoma by Modulating the miR-629-5p/PRDM16 Axis
  JOURNAL   Crit Rev Eukaryot Gene Expr 33 (7), 43-56 (2023)
   PUBMED   37602452
  REMARK    GeneRIF: Long Noncoding RNA MAGI2-AS3 Represses Cell Progression in
            Clear Cell Renal Cell Carcinoma by Modulating the miR-629-5p/PRDM16
            Axis.
REFERENCE   6  (bases 1 to 8698)
  AUTHORS   Nishikata,I., Sasaki,H., Iga,M., Tateno,Y., Imayoshi,S., Asou,N.,
            Nakamura,T. and Morishita,K.
  TITLE     A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
            expressed mainly in t(1;3)(p36;q21)-positive AML and blocks
            G-CSF-induced myeloid differentiation
  JOURNAL   Blood 102 (9), 3323-3332 (2003)
   PUBMED   12816872
  REMARK    GeneRIF: structure, expression pattern, and function of MEL1 in
            leukemia cells; overexpression of the zinc finger protein lacking
            the PR domain (EVI1 and MEL1S)is likely one of the causative
            factors in the pathogenesis of myeloid leukemia.
REFERENCE   7  (bases 1 to 8698)
  AUTHORS   Xinh,P.T., Tri,N.K., Nagao,H., Nakazato,H., Taketazu,F.,
            Fujisawa,S., Yagasaki,F., Chen,Y.Z., Hayashi,Y., Toyoda,A.,
            Hattori,M., Sakaki,Y., Tokunaga,K. and Sato,Y.
  TITLE     Breakpoints at 1p36.3 in three MDS/AML(M4) patients with
            t(1;3)(p36;q21) occur in the first intron and in the 5' region of
            MEL1
  JOURNAL   Genes Chromosomes Cancer 36 (3), 313-316 (2003)
   PUBMED   12557231
  REMARK    GeneRIF: Breakpoints occurring in the first intron and in the 5'
            region of the MEL1 gene associated with the t(1;3)(p36;q21)
            translocation have been found in three myelodysplastic
            syndrome/acute myelogenous leukemia patients.
REFERENCE   8  (bases 1 to 8698)
  AUTHORS   Mochizuki,N., Shimizu,S., Nagasawa,T., Tanaka,H., Taniwaki,M.,
            Yokota,J. and Morishita,K.
  TITLE     A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the
            MDS1/EVI1 gene and is transcriptionally activated in
            t(1;3)(p36;q21)-positive leukemia cells
  JOURNAL   Blood 96 (9), 3209-3214 (2000)
   PUBMED   11050005
REFERENCE   9  (bases 1 to 8698)
  AUTHORS   Secker-Walker,L.M., Mehta,A. and Bain,B.
  TITLE     Abnormalities of 3q21 and 3q26 in myeloid malignancy: a United
            Kingdom Cancer Cytogenetic Group study
  JOURNAL   Br J Haematol 91 (2), 490-501 (1995)
   PUBMED   8547101
REFERENCE   10 (bases 1 to 8698)
  AUTHORS   Bloomfield,C.D., Garson,O.M., Volin,L., Knuutila,S. and de la
            Chapelle,A.
  TITLE     t(1;3)(p36;q21) in acute nonlymphocytic leukemia: a new
            cytogenetic-clinicopathologic association
  JOURNAL   Blood 66 (6), 1409-1413 (1985)
   PUBMED   4063527
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX092842.1, BC161614.1 and
            AL354743.33.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_022114.3.
            
            Summary: The reciprocal translocation t(1;3)(p36;q21) occurs in a
            subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia
            (AML). This gene is located near the 1p36.3 breakpoint and has been
            shown to be specifically expressed in the t(1:3)(p36,q21)-positive
            MDS/AML. The protein encoded by this gene is a zinc finger
            transcription factor and contains an N-terminal PR domain. The
            translocation results in the overexpression of a truncated version
            of this protein that lacks the PR domain, which may play an
            important role in the pathogenesis of MDS and AML. Alternatively
            spliced transcript variants encoding distinct isoforms have been
            reported. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC161614.1, AB051462.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000270722.10/ ENSP00000270722.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BX092842.1         26-50
            26-1653             BC161614.1         1-1628
            1654-1654           AL354743.33        103698-103698
            1655-5739           BC161614.1         1630-5714
            5740-8128           AL354743.33        127567-129955
            8129-8698           BC161614.1         8104-8673
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.32"
     gene            1..8698
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /note="PR/SET domain 16"
                     /db_xref="GeneID:63976"
                     /db_xref="HGNC:HGNC:14000"
                     /db_xref="MIM:605557"
     exon            1..94
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..3888
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /EC_number="2.1.1.367"
                     /note="isoform 1 is encoded by transcript variant 1; PR
                     domain zinc finger protein 16; transcription factor MEL1;
                     MDS1/EVI1-like gene 1; PR domain containing 16; PR domain
                     16; histone-lysine N-methyltransferase PRDM16"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase PRDM16
                     isoform 1"
                     /protein_id="NP_071397.3"
                     /db_xref="CCDS:CCDS41236.2"
                     /db_xref="GeneID:63976"
                     /db_xref="HGNC:HGNC:14000"
                     /db_xref="MIM:605557"
                     /translation="MRSKARARKLAKSDGDVVNNMYEPNRDLLASHSAEDEAEDSAMS
                     PIPVGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKR
                     KMEAGERLGPCVVVPRAAAKETDFGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDA
                     NQAGAGSWLKYIRVACSCDDQNLTMCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLG
                     TVPPGLDEEPTFRCDECDELFQSKLDLRRHKKYTCGSVGAALYEGLAEELKPEGLGGG
                     SGQAHECKDCERMFPNKYSLEQHMVIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSG
                     KRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVK
                     PFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNH
                     YTPGGIFAPGLPLTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFP
                     ALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQDAKLPSPLGNPALPLVSAVSNSS
                     QGTTAAAGPEEKFESRLEDSCVEKLKTRSSDMSDGSDFEDVNTTTGTDLDTTTGTGSD
                     LDSDVDSDPDKDKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQL
                     LTATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPF
                     TDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASG
                     EEQPLDLSIGSRARASQNGGGREPRKNHVYGERKLGAGEGLPQVCPARMPQQPPLHYA
                     KPSPFFMDPIYSRVEKRKVTDPVGALKEKYLRPSPLLFHPQMSAIETMTEKLESFAAM
                     KADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRT
                     HTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH
                     EHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQA
                     STRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDDLEEDDEDSLAGKSQDDTVSPA
                     PEPQAAYEDEEDEEPAASLAVGFDHTRRCAEDHEGGLLALEPMPTFGKGLDLRRAAEE
                     AFEVKDVLNSTLDSEALKHTLCRQAKNQAYAMMLSLSEDTPLHTPSQGSLDAWLKVTG
                     ATSESGAFHPINHL"
     exon            95..444
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            445..495
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            496..630
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            631..733
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            734..941
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1089
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1243
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..2660
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2748
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2918
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            2919..2996
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3166
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3341
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3578
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3753
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     exon            3754..8698
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6239..6244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /note="hexamer: ATTATA"
     polyA_site      6261
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /note="major polyA site"
     regulatory      8676..8681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /note="hexamer: AATAAA"
     polyA_site      8698
                     /gene="PRDM16"
                     /gene_synonym="CMD1LL; KMT8F; LVNC8; MEL1; PFM13"
                     /note="major polyA site"
ORIGIN      
        1 agtgtgtggc tgcttctgga ctcaaggagg aggagagaga ttccgcgagc cgacaccatg
       61 cgatccaagg cgagggcgag gaagctagcc aaaagtgacg gtgacgttgt aaataatatg
      121 tatgagccca accgggacct gctggccagc cacagcgcgg aggacgaggc cgaggacagt
      181 gccatgtcgc ccatccccgt ggggccaccg tcccccttcc ccaccagcga ggacttcacc
      241 cccaaggagg gctcgccgta cgaggcccct gtctacattc ctgaagacat tccgatccca
      301 gcagacttcg agctccgaga gtcctccatc ccaggggctg gcctgggggt ctgggccaag
      361 aggaagatgg aagccgggga gaggctgggc ccctgcgtgg tggtgccccg ggcggcggca
      421 aaggagacag acttcggatg ggagcaaata ctgacggacg tggaagtgtc gccccaggaa
      481 ggctgcatca caaagatctc cgaagacctg ggcagtgaga agttctgcgt ggatgcaaat
      541 caggcggggg ctggcagctg gctcaagtac atccgtgtgg cgtgctcctg cgatgaccag
      601 aacctcacca tgtgtcagat cagtgagcag atttactata aagtcattaa ggacattgag
      661 ccaggtgagg agctgctggt gcacgtgaag gaaggcgtct accccctggg cacagtgccg
      721 cccggcctgg acgaggagcc cacgttccgc tgtgacgagt gtgacgaact cttccagtcc
      781 aagctggacc tgcggcgcca taagaagtac acgtgtggct cagtgggggc tgcgctctac
      841 gagggcctgg ctgaggagct caagcccgag ggccttggcg gtggcagcgg ccaagcccac
      901 gagtgcaagg actgcgagcg gatgttcccc aacaagtaca gcctggagca gcacatggtc
      961 atccacacgg aggagcgcga gtacaaatgc gaccagtgtc ccaaggcctt caactggaag
     1021 tccaacctca tccgccacca gatgtcccac gacagcggca aacgcttcga atgtgaaaac
     1081 tgcgtgaagg tgttcacgga ccccagcaac cttcagcggc acatccgctc gcagcacgtg
     1141 ggcgctcggg cccacgcctg ccccgactgc gggaagacct tcgccacgtc ctccggcctc
     1201 aagcagcaca agcatatcca cagcacggtg aagcctttca tatgtgaggt ctgccacaag
     1261 tcctacacgc agttctccaa cctgtgccgg cacaagcgga tgcacgccga ctgccgcacg
     1321 cagatcaagt gcaaggactg tggccagatg ttcagcacta cctcctccct caacaagcac
     1381 cggcgcttct gcgagggcaa gaaccattac acgccgggcg gcatctttgc cccgggcctg
     1441 cccttgaccc ccagccccat gatggacaag gcaaaaccct cccccagcct caatcacgcc
     1501 agcctgggct tcaacgagta ctttccctcc aggccgcacc cggggagcct gcccttctcc
     1561 acggcgcctc ccacgttccc cgcactcacc cccggcttcc cgggcatctt ccctccatcc
     1621 ttgtaccccc ggccgcctct gctacctccc acatcgctgc tcaagagccc cctgaaccac
     1681 acccaggacg ccaagctccc cagtcccctg gggaacccag ccctgcccct ggtctccgcc
     1741 gtcagcaaca gcagccaggg cacgacggca gctgcggggc ccgaggagaa gttcgagagc
     1801 cgcctggagg actcctgtgt ggagaagctg aagaccagga gcagcgacat gtcggacggc
     1861 agtgactttg aggacgtcaa caccaccacg gggaccgacc tggacacgac cacggggacg
     1921 ggctcggacc tggacagcga cgtggacagc gaccctgaca aggacaaggg caagggcaag
     1981 tccgccgagg gccagcccaa gtttgggggc ggcttggcgc ccccgggggc cccgaacagc
     2041 gtggccgagg tgcctgtctt ctattcccag cactcattct tcccgccacc cgacgagcag
     2101 ctgctgactg caacgggcgc cgccggggac tccatcaagg ccatcgcatc cattgccgag
     2161 aagtactttg gccccggctt catggggatg caggagaaga agctgggctc gctcccctac
     2221 cactcggcgt tccccttcca gttcctgccc aacttccccc actcccttta ccccttcacg
     2281 gaccgagccc tcgcccacaa cttgctggtc aaggccgagc caaagtcacc ccgggacgcc
     2341 ctcaaggtgg gcggccccag tgccgagtgc ccctttgatc tcaccaccaa gcccaaagac
     2401 gtgaagccca tcctgcccat gcccaagggc ccctcggccc ccgcatccgg cgaggagcag
     2461 ccgctggacc tgagcatcgg cagccgggcc cgtgccagcc aaaacggcgg cgggcgggag
     2521 ccccgcaaga accacgtcta tggggaacgc aagctgggcg ccggcgaggg gctgccccag
     2581 gtgtgcccgg cgcggatgcc ccagcagccc ccgctccact acgccaagcc ctcgcccttc
     2641 ttcatggacc ccatctacag cagggtagaa aagcggaagg tcacagaccc cgtgggagcc
     2701 ctgaaggaga agtacctgcg gccgtccccg ctgctcttcc acccccagat gtcagccata
     2761 gagaccatga cagagaagct ggagagcttt gcagccatga aggcggactc gggcagctcc
     2821 ctgcagcccc tcccccacca ccccttcaac ttccggtccc cacccccaac gctctccgac
     2881 cccatcctca ggaagggcaa ggagcgatac acgtgcaggt actgtgggaa gatcttcccc
     2941 agatcagcca atctcaccag acacctgagg acgcacactg gggagcagcc gtacaggtgt
     3001 aagtactgcg accgctcctt cagcatctct tcgaacctcc agcggcacgt ccggaacatc
     3061 cacaacaagg agaagccttt caagtgccac ctgtgcaacc gctgcttcgg gcagcagacc
     3121 aacctggacc ggcacctcaa gaagcacgag cacgagaacg caccagtgag ccagcacccc
     3181 ggggtcctca cgaaccacct ggggaccagc gcgtcctctc ccacctcaga gtcggacaac
     3241 cacgcacttt tagacgagaa agaagactct tatttctcgg aaatcagaaa ctttattgcc
     3301 aatagtgaga tgaaccaagc atcaacgcga acagagaaac gggcggacat gcagatcgtg
     3361 gacggcagtg cccagtgtcc aggcctagcc agtgagaagc aggaggacgt ggaggaggag
     3421 gacgacgatg acctggagga ggacgatgag gacagcctgg ccgggaagtc gcaggatgac
     3481 accgtgtccc ccgcacccga gccccaggcc gcctacgagg atgaggagga tgaggagcca
     3541 gccgcctccc tggccgtggg ctttgaccac acccgaaggt gtgctgagga ccacgaaggc
     3601 ggtctgttag ctttggagcc gatgccgact tttgggaagg ggctggacct ccgcagagca
     3661 gctgaggaag catttgaagt taaagatgtg cttaattcca ccttagattc tgaggcttta
     3721 aaacatacac tgtgcaggca ggctaagaac caggcatatg caatgatgct gtccctttcc
     3781 gaagacactc ctctccacac cccctcccag ggttctctgg acgcttggtt gaaggtcact
     3841 ggagccacgt cggagtctgg agcatttcac cccatcaacc acctctgacg ggctgggcag
     3901 ccgggggccg gtggccagag cgagggcacc agccacgaag gacggaggcg ggcggggccc
     3961 cggagaaccc tgtccctgcg tgtggccact cctcagcatc ctccccaccc accatggttc
     4021 attccgactt ttccaatgga aactcagatc ccaaaagtcc ctaaagcagt cgtagagtct
     4081 caccatctcc aaggattggt cttgagaaca ctgttcagtg acggccatgc aggtggccgt
     4141 ccaaagacag ccaacggagc tgcctcgcag aatcagccag tgggcaggtg gacgctctgc
     4201 tgagacagaa gctggtggcc actgccgggt gcccgcgtgg ggtcgcggaa gggaatggat
     4261 agactggtgt gctcaaaaga gagagatcac tcaaatgatt tttataatga aatgacaaga
     4321 ataacccttt tggtaaccgt attgactgca gagtctattt aagcatgtgg ttttaaaaat
     4381 agacagtatt ttttaaaaat caaaaaatga cttgcaaatt gttttttaaa agtaattttg
     4441 cattgctttg aaatttgagc tcatttgcaa acccgagtct gcctgggaac ccgcactgtg
     4501 cctgggtgta ttctttatac tgtagataat ggagaaattt tctatctctg tccctatttg
     4561 tataagccaa ggtgatgctg ggtgccccga ggcagaacaa gaggcgcggg gccacacccg
     4621 tgaaccatgc agacggccga agaagtctta ggcagggcgc cctgggctgc aggcctgccc
     4681 gaggctggga tgggaagtgt gcctgccctc gtgtgacatg gaattggtgt caggaccgcc
     4741 acgtggcctt cagaggaatc cacaggtccc cacccaagat ccctcaatta tatggggaag
     4801 tcgagggcct gtggcttgga tccgccatgc agagatgtgg ccgggcaccc atcttccttc
     4861 cctcctctgt ccctgcctcg gccaccccac gcgggaaccc agcgccgtcc tctgaaggca
     4921 gggccttggc cacgtcctgg gtctcccacc tcccacctga ccccagcggc tccggtgtcc
     4981 tccacgtggc tgccctgggg agcaatccca gcggatcgct ccgggccacc aagccgcacc
     5041 tgtgcctgag actccggatg gacgacacag tcgtcacgtc gctcttcctg cgggttcttg
     5101 gcgagacaca gcttgagaac agaagggcgt cgggggaacc tgccgcaagg agcagagaca
     5161 gcacagcccc ccgggcccag ccgcctccct ctcttgggac gcaacttctt ccccactcgg
     5221 atgggcttta aattattccc ataggggcca atttcaaata ataatttttt tccctgatgg
     5281 aatttacctt aatctgtata taacttgtaa ttttttctaa ttcatttctt ttcttatttt
     5341 atttcctcct taacagtatt tttggcatta gacattctta ttgtgaagaa ataatgttaa
     5401 tataagtatc tggtgaagga ccaaaaccgt gtgataaggt tgtgtgtcgt gtgggagtgg
     5461 ggcgattttt tatgtgccaa atacccccgt cccccccatg aatcctgctg tccctgctgc
     5521 cgtttaccag acaatcatat gtttttgtta aatttgcgtt tcagttacat ttgcatttaa
     5581 gacaagtgtt ctatttattt cttgtattgt ttggaagaaa aaatgatgat agagtcccaa
     5641 aaagaagaga aaaaaaatgc ccaagttgcc ctttaaaaaa aaagagcgta aatacaaaca
     5701 ggagtggtgc aagccgcctt ggtgtgggtt tgtgtcacgt gtggacatct cctcaggctt
     5761 tgtgtcacgc gtggacatct cctcaggctg tccccagcgg tgacgggagg tgtcctggct
     5821 gctccaggac aaaagacaat cgtctctgtg ggtgccgggt ggtccaggct tgcactgaag
     5881 acgtgccacg gggaggctcc tgcaggaggc tcaacccgac ggatcacagt gaaagggatt
     5941 cctcccacgc cagatctgca caacgaggca agacaggacc cacctgtgcg tgcgctgggg
     6001 ccatggggtg gccccgccgg ggcagcgggg gagctgcctg cagaagagcc agctggcgtg
     6061 tcgggaagga tccaggatct gcaaacacaa ctgctcaggc cttctcacgc gtttccacaa
     6121 catcccctgg gtcagaccca ccaggtaccc cgtaggaatt tccagtttcc cttgatctag
     6181 atgggattct tataaaaatt caacctcaga cataaacacc ccatttctgt aaacccaaat
     6241 tatatggttt cttctgcgaa agagtaaggt gtgtgctttt ttttttttgc aatatgaccc
     6301 cgtctctctg aagtgggaca ttcggacgga tggagccctc agcgtgtctt ttcagcagga
     6361 gcagaaccga tgagagccgc ccttaccgtt ggtctccgga tcccccagtc ccatcccgcc
     6421 gttttcggct gtcttcctaa ccgtcctgtc ttctcttggc gctctttcct tccacctttc
     6481 ccaagagtcc tggttgcacg ttttaagtca tatattttcg tccccctgaa aatgatggca
     6541 agcccagttt ctcctgagca ttcagacccc caggccccag cacttggcgt tttcaggagg
     6601 ccctgttctt agagcccctg acaaaggcag cacttatttc ctgggctggt gcgccccaaa
     6661 acacggcccc gacacttagt gtggccccag gccccagcga gcctcgccct cccagttttg
     6721 ctctgcccag cagtgttggt gcccagagat gacaagggcc agggagcctg gcccgggtgt
     6781 gagaattcag agattctggc ctccagctgt caccacaccg taacggggcc atgtaactgt
     6841 gcagcatgga cagggatgcg acggggcagc tggctgtgtc catggccagg tggccagggt
     6901 cagggctgca agccaggggt ccagggccct tccgttcagc ccaaatgctg ccccaatgct
     6961 aactccttgg attgtcaacc cccatccccc aaatggaaat tccgaaggag gcctcctcgc
     7021 acctgccctc cgctgctcct cagaccccag cccccagcga gccgacgtcc ccacccgttc
     7081 ctgctctcat ccccaggttg ggcacgtggg gttcctcctc tgtgggcctg gcagaccctt
     7141 catgagtggg acccaagata tcactgactt caacccagag gatcgagccc ctgcaccctg
     7201 cctggggccc tggggtgtgg agcagtggct ggggtgggcg tggtgtggcc tgagagactg
     7261 cccagctgga gaggccttcc tttacaaggc cacgcgtgca gctgtcccat ccagaccccg
     7321 actggccaag acctccacgt ccccagagtc cagccctgga aattccaagg gccctggcgt
     7381 cctctgcctt ccccgcttcc ccatgagcgt ctgcaaaaca cttgcctgaa tacatatcac
     7441 gtattttaga ctcgaagcct caaagcactg gattgtggtc ccctgccccc tctgtcccgt
     7501 ccccctgccc aagtgactga aacctactga gctatattca ctgtgctgtc ctagggggag
     7561 ggagagcaga gctcgcccct gcactgcagc cttgtggggg agggcaaggc tctcctccca
     7621 gccagggacg ccaggacata gctgctcctg gtcagtggag gtcagccggg tatcaaaagc
     7681 catgaaactg tgtctctgta gcaatgagtg atactgtgac aaaaccatcc ttgcattctt
     7741 cctagaagag ttcctctgct ccttccattc catttttgtg tttgttttgt tcttttctgt
     7801 cactgatccg tattaccact tttggaaaaa aataaataaa taaataaata aaaggcagct
     7861 tgagtttcca aacgtgtgat tcacttgtga acaaaagtca ttctaacaat tgccttcagc
     7921 gtcacgtgca ttgccactgc gctttcggca cgagggatgc tgagccctgg tgtcagagtc
     7981 gtaatttaaa gcgtgtgtgt atatggactt tgtcccttaa ggtcgatata aagaatcctc
     8041 gcagaatcac agacctgtgc cgcccgccac cttctgccat tgttacatta cagatttggt
     8101 ttagttttgt tttgttttgt tttttctttt agaactgtat agtattgaaa aagaaatcaa
     8161 atgtaaatgt ctggttttca tataatgttt aaaaagacca ttgagaagga ggctggcgct
     8221 cgccccatgt cccccttgat tgtaaattgc ttctgttctg tttataagta aactgtgcat
     8281 gactcctgct tagcggtcat tatcgtgtct gttggtgaaa tttttattaa aaggaaaatt
     8341 ctgtagatgc acttattgaa tatgtgatta ggatctacgt ctgagactag gagtcctgaa
     8401 ctgctgacgc gaaagaggcg cagttcccaa ttaatacgga aatcgctgtg ggagaagaat
     8461 gaaataagac gtgaagtgta ggaaatcatg aaaagaacaa ttttgcaaat tgcattctga
     8521 tgcttgtgat gaacacaaat gtacttgtgt agagacattt ccttaagaga aagcctagga
     8581 gaagccgatt tggaggttaa tgctgtagaa taggactgta taccaaatgt aatctttcca
     8641 atgctccaat gaatttatac atgagattga tatgcaataa atctgtgtgc ttttctaa
//
