LOCUS       NM_005040               3489 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1,
            mRNA.
ACCESSION   NM_005040
VERSION     NM_005040.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Wu,Y., Pan,X. and Jin,X.
  TITLE     Haplotype-based association study between PRCP gene polymorphisms
            and essential hypertension in Hani minority group from a remote
            region of China
  JOURNAL   J Renin Angiotensin Aldosterone Syst 21 (4), 1470320320981316
            (2020)
   PUBMED   33319614
  REMARK    GeneRIF: Haplotype-based association study between PRCP gene
            polymorphisms and essential hypertension in Hani minority group
            from a remote region of China.
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Wu,Y., Yang,H. and Xiao,C.
  TITLE     Genetic association study of prolylcarboxypeptidase polymorphisms
            with susceptibility to essential hypertension in the Yi minority of
            China: A case-control study based on an isolated population
  JOURNAL   J Renin Angiotensin Aldosterone Syst 21 (2), 1470320320919586
            (2020)
   PUBMED   32448049
  REMARK    GeneRIF: Genetic association study of prolylcarboxypeptidase
            polymorphisms with susceptibility to essential hypertension in the
            Yi minority of China: A case-control study based on an isolated
            population.
REFERENCE   3  (bases 1 to 3489)
  AUTHORS   Bayer,M., Tsiskarishvili,N., Stegemann,A., Bohm,M. and Konig,S.
  TITLE     Fast oxidation of alpha-melanocyte-stimulating hormone and derived
            peptides under laboratory conditions causes irreproducible
            results-Insights from studies of prolylcarboxypeptidase in human
            cell types
  JOURNAL   Pigment Cell Melanoma Res 33 (2), 378-382 (2020)
   PUBMED   31837203
  REMARK    GeneRIF: Fast oxidation of alpha-melanocyte-stimulating hormone and
            derived peptides under laboratory conditions causes irreproducible
            results-Insights from studies of prolylcarboxypeptidase in human
            cell types.
REFERENCE   4  (bases 1 to 3489)
  AUTHORS   Krochmal,M., Cisek,K., Filip,S., Markoska,K., Orange,C.,
            Zoidakis,J., Gakiopoulou,C., Spasovski,G., Mischak,H., Delles,C.,
            Vlahou,A. and Jankowski,J.
  TITLE     Identification of novel molecular signatures of IgA nephropathy
            through an integrative -omics analysis
  JOURNAL   Sci Rep 7 (1), 9091 (2017)
   PUBMED   28831120
  REMARK    GeneRIF: Using multiple -omics approach, study validated key
            molecules, such as CAP1, SHC1 and PRCP, that might play a
            significant role in IgA nephropathy pathogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3489)
  AUTHORS   Kehoe,K., Van Elzen,R., Verkerk,R., Sim,Y., Van der Veken,P.,
            Lambeir,A.M. and De Meester,I.
  TITLE     Prolyl carboxypeptidase purified from human placenta: its
            characterization and identification as an apelin-cleaving enzyme
  JOURNAL   Biochim Biophys Acta 1864 (11), 1481-1488 (2016)
   PUBMED   27449720
  REMARK    GeneRIF: For the first time, PRCP is identified as an
            apelin-cleaving enzyme.
REFERENCE   6  (bases 1 to 3489)
  AUTHORS   Watson,B. Jr., Nowak,N.J., Myracle,A.D., Shows,T.B. and
            Warnock,D.G.
  TITLE     The human angiotensinase C gene (HUMPCP) maps to 11q14 within 700
            kb of D11S901: a candidate gene for essential hypertension
  JOURNAL   Genomics 44 (3), 365-367 (1997)
   PUBMED   9325062
REFERENCE   7  (bases 1 to 3489)
  AUTHORS   Jackman,H.L., Tan,F., Schraufnagel,D., Dragovic,T., Dezso,B.,
            Becker,R.P. and Erdos,E.G.
  TITLE     Plasma membrane-bound and lysosomal peptidases in human alveolar
            macrophages
  JOURNAL   Am J Respir Cell Mol Biol 13 (2), 196-204 (1995)
   PUBMED   7626287
REFERENCE   8  (bases 1 to 3489)
  AUTHORS   Tan,F., Morris,P.W., Skidgel,R.A. and Erdos,E.G.
  TITLE     Sequencing and cloning of human prolylcarboxypeptidase
            (angiotensinase C). Similarity to both serine carboxypeptidase and
            prolylendopeptidase families
  JOURNAL   J Biol Chem 268 (22), 16631-16638 (1993)
   PUBMED   8344943
  REMARK    Erratum:[J Biol Chem 1993 Dec 5;268(34):26032]
REFERENCE   9  (bases 1 to 3489)
  AUTHORS   Odya,C.E., Marinkovic,D.V., Hammon,K.J., Stewart,T.A. and
            Erdos,E.G.
  TITLE     Purification and properties of prolylcarboxypeptidase
            (angiotensinase C) from human kidney
  JOURNAL   J Biol Chem 253 (17), 5927-5931 (1978)
   PUBMED   28321
REFERENCE   10 (bases 1 to 3489)
  AUTHORS   Yang,H.Y., Erdos,E.G. and Chiang,T.S.
  TITLE     New enzymatic route for the inactivation of angiotensin
  JOURNAL   Nature 218 (5148), 1224-1226 (1968)
   PUBMED   4297812
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI827978.1, BC001500.2,
            AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005040.3.
            
            Summary: This gene encodes a member of the peptidase S28 family of
            serine exopeptidases. The encoded preproprotein is proteolytically
            processed to generate the mature lysosomal prolylcarboxypeptidase.
            This enzyme cleaves C-terminal amino acids linked to proline in
            peptides such as angiotension II, III and des-Arg9-bradykinin. The
            cleavage occurs at acidic pH, but the enzyme activity is retained
            with some substrates at neutral pH. This enzyme has been shown to
            be an activator of the cell matrix-associated prekallikrein. The
            importance of angiotension II, one of the substrates of this
            enzyme, in regulating blood pressure and electrolyte balance
            suggests that this gene may be related to essential hypertension. A
            pseudogene of this gene has been identified on chromosome 2.
            Alternative splicing results in multiple transcript variants, at
            least one of which encodes an isoform that is proteolytically
            processed. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.158416.1,
                                           SRR1803612.257561.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000313010.8/ ENSP00000317362.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BI827978.1         168-176
            10-1933             BC001500.2         1-1924
            1934-2117           AA477618.1         10-193              c
            2118-2372           BE154586.1         110-364             c
            2373-2882           CB154314.1         81-590
            2883-3283           AP001646.5         62032-62432         c
            3284-3485           AW627417.1         13-214              c
            3486-3489           AP001646.5         61826-61829         c
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q14.1"
     gene            1..3489
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="prolylcarboxypeptidase"
                     /db_xref="GeneID:5547"
                     /db_xref="HGNC:HGNC:9344"
                     /db_xref="MIM:176785"
     exon            1..196
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1519
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /EC_number="3.4.16.2"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; angiotensinase C; lysosomal Pro-x
                     carboxypeptidase; proline carboxypeptidase; lysosomal
                     carboxypeptidase C"
                     /codon_start=1
                     /product="lysosomal Pro-X carboxypeptidase isoform 1
                     preproprotein"
                     /protein_id="NP_005031.1"
                     /db_xref="CCDS:CCDS8262.1"
                     /db_xref="GeneID:5547"
                     /db_xref="HGNC:HGNC:9344"
                     /db_xref="MIM:176785"
                     /translation="MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAV
                     AKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNN
                     TGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKH
                     LKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVF
                     MKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLK
                     DWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVY
                     YNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSD
                     DCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTI
                     SEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH"
     exon            197..337
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            338..439
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            440..621
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            622..779
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            780..949
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1114
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1302
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..3489
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAAA"
     polyA_site      2058
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="major polyA site"
     regulatory      3287..3292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAGA"
     polyA_site      3307
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
     regulatory      3455..3460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAAA"
     polyA_site      3489
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
ORIGIN      
        1 acccgcactg cagtctccag cctgagccat gggccgccga gccctcctgc tcctgcttct
       61 gtcttttctg gcgccctggg ccaccatagc cctccggccg gccttaaggg ccctcggcag
      121 cctacacttg ccaaccaacc ccacatccct cccggctgta gccaagaact attcggttct
      181 ctacttccaa cagaaggttg atcattttgg atttaatact gtgaaaactt ttaatcagcg
      241 gtacctagta gctgataaat actggaagaa aaatggtgga tcaatacttt tctacactgg
      301 taatgaaggg gacattatct ggttttgtaa taacacgggg ttcatgtggg atgtggctga
      361 ggaactgaaa gctatgttgg tgtttgctga acatcgatac tatggagagt ctctcccctt
      421 tggtgacaac tcattcaagg attccagaca cttgaatttc ctgacatcag aacaagctct
      481 ggctgatttt gcagagttaa tcaaacactt gaaaagaaca atcccaggag ctgaaaatca
      541 acctgtcatt gccataggag gctcctatgg tggcatgctt gccgcctggt ttaggatgaa
      601 atatcctcat atggtagttg gagctcttgc agcttctgcc cctatctggc agtttgagga
      661 tttagtacct tgtggtgtat ttatgaagat cgtaactaca gattttagga aaagcggtcc
      721 acattgttca gagagcatcc acaggtcctg ggatgccatt aatcgactct caaatactgg
      781 cagtggtttg cagtggctta ctggagccct tcacttatgc agcccattaa cttctcagga
      841 catccaacat ttgaaagact ggatctctga aacctgggtg aatctggcaa tggtggacta
      901 tccttatgcc tctaactttt tacagccttt gcctgcttgg cctatcaagg tagtgtgcca
      961 gtatttgaaa aatcccaatg tatctgattc actgctgctg cagaatattt tccaagctct
     1021 gaatgtatat tacaattatt cgggccaggt gaaatgcctg aatatttcag agacagcaac
     1081 tagcagtctg ggaacactgg gttggagcta tcaggcctgc acagaagtag tcatgccctt
     1141 ttgtactaat ggtgtcgatg acatgtttga acctcactca tggaacttaa aggaactttc
     1201 tgatgactgt tttcaacagt ggggtgtgag accaaggccc tcctggatca ctactatgta
     1261 tggaggcaaa aacattagtt cacacacaaa cattgttttc agcaatggtg aactagaccc
     1321 ctggtcagga ggtggagtaa ctaaggatat cacagacact ctggttgcag tcaccatctc
     1381 agagggggcc caccacttag atctccgcac caagaatgcc ttggatccta tgtctgtgct
     1441 gttagcccgc tccttggaag ttagacatat gaagaattgg atcagagatt tctatgacag
     1501 tgcgggaaag cagcactgag aaacttttga ttgttttcaa tttcttcttt tatgttcaca
     1561 ccaccacatt cccattcact ttgattttct acatgtaatt accttctttt gtttatcatt
     1621 agatttgatg gggccaaagt tgagatagaa tagagggtga tgacggtaag agcaagtgtc
     1681 ccatgaatgt gatttcctgg gttctcactg tctttgcacc acgtctagga agaatcttct
     1741 tgatagctct cccacaccat cagtggccct cataactgga gtagagttcc tggttgcttt
     1801 tcataagagg gagagttact ttctttgtat ctctgcaagc agagatttct ctttggtttt
     1861 gaggttgaag tgtctttggc ccatttgtaa gtccccatcc ctaccctaca caaagtaaaa
     1921 gcagaagata gataaaaaat gatgtaattg cagctggtag gatgtctggt gccaatccag
     1981 gaagtgagag ccatttcttt tgtaccggat ttaatgactt tgaactgtgc tgtaaataaa
     2041 taatacagct ggaccttata cctgtgtgtt gcttctgaag ctcacttcaa ctccggctga
     2101 tggaaatgct gtgtgcactg ggcttagctg tttttggaaa gtcctttaat tttttctctt
     2161 ccacttggca gcttttctta gttctgggat gttggccact gtggtactgc cagtaagaaa
     2221 tctggccttt ttctgctcca tttctctttg tttctaaggt cagttttaat ttgtttgaat
     2281 tctgccagtt taggaaaatg actctgtttt gtctttttaa atctgagcaa gctttatgct
     2341 gtagtcacta attatatttc agtaagaagc ctttaaaaat tgatgaatca gctattccaa
     2401 aagtagaata tagtaatatt cagcttttaa atttttaaaa ttttattttc tgatagttgt
     2461 tactgctgtc attaaaacca gaagtgttag acactaacag gcaggtgcac aagaactttt
     2521 ctgtatgact tccaaaagac aagctgacta attcatgcag gcagcatatt tgacattaat
     2581 aaatggccaa gccattccta tttccaaggg gttggcttct ttcaagcagt tcttacactg
     2641 agacctttct tcagagagct tttctaggat aacaaatagg gttggatcaa aacctttcag
     2701 ggacacagag agacagagag agagagacta acagtttcta ctctgttgaa agatttcttc
     2761 ctccttgttt cttttcattt tctctcaata attttttttt cttcaccctg ccttgggaac
     2821 attttctgct tttaaaaaac tgtaccttga gcttgatcat ttttatggca tttttttttt
     2881 ttaagggcat atatagagtt cattcttggg atggaaagct gaaatatttc ctttactttc
     2941 cctggcatct gggagataca ggaaaatctt tgtcttattt tgggagcctt cagttctcat
     3001 tgtaatgagt tgtctatcaa agcatttggt aactattttg tttcagcatc acagaacaat
     3061 cgatttgggt gggaatttaa gggccaaaac aatactagtt tttattcttt gtcaactaag
     3121 aaattaaata atgcagggtt tatgggctca atagaggata ttcttacaat ttattttacg
     3181 attttagggt ttttcccttg tgtagaatct ttaaaatact ttgccatttt atttgatgtt
     3241 gattatttgg atgagttaca ttattctgaa gaaacaaact gcaaaaaata gaattttttg
     3301 gcaagcataa ttgtatccta ttactgtatg cattttagga atatagatag cagccttaat
     3361 tgaagtgagt ataattataa tatgctaagg tttaatttta acatgaaaaa gaattggagt
     3421 gtcagataag ccttattatt taaagcatcc ctctaataaa actgcacttt ttactgacag
     3481 ggaaagaga
//
