LOCUS       NM_001319214            3204 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 3,
            mRNA.
ACCESSION   NM_001319214
VERSION     NM_001319214.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wu,Y., Pan,X. and Jin,X.
  TITLE     Haplotype-based association study between PRCP gene polymorphisms
            and essential hypertension in Hani minority group from a remote
            region of China
  JOURNAL   J Renin Angiotensin Aldosterone Syst 21 (4), 1470320320981316
            (2020)
   PUBMED   33319614
  REMARK    GeneRIF: Haplotype-based association study between PRCP gene
            polymorphisms and essential hypertension in Hani minority group
            from a remote region of China.
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wu,Y., Yang,H. and Xiao,C.
  TITLE     Genetic association study of prolylcarboxypeptidase polymorphisms
            with susceptibility to essential hypertension in the Yi minority of
            China: A case-control study based on an isolated population
  JOURNAL   J Renin Angiotensin Aldosterone Syst 21 (2), 1470320320919586
            (2020)
   PUBMED   32448049
  REMARK    GeneRIF: Genetic association study of prolylcarboxypeptidase
            polymorphisms with susceptibility to essential hypertension in the
            Yi minority of China: A case-control study based on an isolated
            population.
REFERENCE   3  (bases 1 to 3204)
  AUTHORS   Bayer,M., Tsiskarishvili,N., Stegemann,A., Bohm,M. and Konig,S.
  TITLE     Fast oxidation of alpha-melanocyte-stimulating hormone and derived
            peptides under laboratory conditions causes irreproducible
            results-Insights from studies of prolylcarboxypeptidase in human
            cell types
  JOURNAL   Pigment Cell Melanoma Res 33 (2), 378-382 (2020)
   PUBMED   31837203
  REMARK    GeneRIF: Fast oxidation of alpha-melanocyte-stimulating hormone and
            derived peptides under laboratory conditions causes irreproducible
            results-Insights from studies of prolylcarboxypeptidase in human
            cell types.
REFERENCE   4  (bases 1 to 3204)
  AUTHORS   Krochmal,M., Cisek,K., Filip,S., Markoska,K., Orange,C.,
            Zoidakis,J., Gakiopoulou,C., Spasovski,G., Mischak,H., Delles,C.,
            Vlahou,A. and Jankowski,J.
  TITLE     Identification of novel molecular signatures of IgA nephropathy
            through an integrative -omics analysis
  JOURNAL   Sci Rep 7 (1), 9091 (2017)
   PUBMED   28831120
  REMARK    GeneRIF: Using multiple -omics approach, study validated key
            molecules, such as CAP1, SHC1 and PRCP, that might play a
            significant role in IgA nephropathy pathogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3204)
  AUTHORS   Kehoe,K., Van Elzen,R., Verkerk,R., Sim,Y., Van der Veken,P.,
            Lambeir,A.M. and De Meester,I.
  TITLE     Prolyl carboxypeptidase purified from human placenta: its
            characterization and identification as an apelin-cleaving enzyme
  JOURNAL   Biochim Biophys Acta 1864 (11), 1481-1488 (2016)
   PUBMED   27449720
  REMARK    GeneRIF: For the first time, PRCP is identified as an
            apelin-cleaving enzyme.
REFERENCE   6  (bases 1 to 3204)
  AUTHORS   Watson,B. Jr., Nowak,N.J., Myracle,A.D., Shows,T.B. and
            Warnock,D.G.
  TITLE     The human angiotensinase C gene (HUMPCP) maps to 11q14 within 700
            kb of D11S901: a candidate gene for essential hypertension
  JOURNAL   Genomics 44 (3), 365-367 (1997)
   PUBMED   9325062
REFERENCE   7  (bases 1 to 3204)
  AUTHORS   Jackman,H.L., Tan,F., Schraufnagel,D., Dragovic,T., Dezso,B.,
            Becker,R.P. and Erdos,E.G.
  TITLE     Plasma membrane-bound and lysosomal peptidases in human alveolar
            macrophages
  JOURNAL   Am J Respir Cell Mol Biol 13 (2), 196-204 (1995)
   PUBMED   7626287
REFERENCE   8  (bases 1 to 3204)
  AUTHORS   Tan,F., Morris,P.W., Skidgel,R.A. and Erdos,E.G.
  TITLE     Sequencing and cloning of human prolylcarboxypeptidase
            (angiotensinase C). Similarity to both serine carboxypeptidase and
            prolylendopeptidase families
  JOURNAL   J Biol Chem 268 (22), 16631-16638 (1993)
   PUBMED   8344943
  REMARK    Erratum:[J Biol Chem 1993 Dec 5;268(34):26032]
REFERENCE   9  (bases 1 to 3204)
  AUTHORS   Odya,C.E., Marinkovic,D.V., Hammon,K.J., Stewart,T.A. and
            Erdos,E.G.
  TITLE     Purification and properties of prolylcarboxypeptidase
            (angiotensinase C) from human kidney
  JOURNAL   J Biol Chem 253 (17), 5927-5931 (1978)
   PUBMED   28321
REFERENCE   10 (bases 1 to 3204)
  AUTHORS   Yang,H.Y., Erdos,E.G. and Chiang,T.S.
  TITLE     New enzymatic route for the inactivation of angiotensin
  JOURNAL   Nature 218 (5148), 1224-1226 (1968)
   PUBMED   4297812
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK302386.1, BC001500.2,
            AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001319214.1.
            
            Summary: This gene encodes a member of the peptidase S28 family of
            serine exopeptidases. The encoded preproprotein is proteolytically
            processed to generate the mature lysosomal prolylcarboxypeptidase.
            This enzyme cleaves C-terminal amino acids linked to proline in
            peptides such as angiotension II, III and des-Arg9-bradykinin. The
            cleavage occurs at acidic pH, but the enzyme activity is retained
            with some substrates at neutral pH. This enzyme has been shown to
            be an activator of the cell matrix-associated prekallikrein. The
            importance of angiotension II, one of the substrates of this
            enzyme, in regulating blood pressure and electrolyte balance
            suggests that this gene may be related to essential hypertension. A
            pseudogene of this gene has been identified on chromosome 2.
            Alternative splicing results in multiple transcript variants, at
            least one of which encodes an isoform that is proteolytically
            processed. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (3) differs in the the 5' UTR,
            lacks a portion of the 5' coding region and initiates translation
            at a downstream start codon compared to variant 1. The encoded
            isoform (3) has a shorter N-terminus and lacks a predicted signal
            peptide compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302386.1, SRR18074969.3081359.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1218              AK302386.1         48-1265
            1219-1648           BC001500.2         1495-1924
            1649-1832           AA477618.1         10-193              c
            1833-2087           BE154586.1         110-364             c
            2088-2597           CB154314.1         81-590
            2598-2998           AP001646.5         62032-62432         c
            2999-3200           AW627417.1         13-214              c
            3201-3204           AP001646.5         61826-61829         c
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q14.1"
     gene            1..3204
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="prolylcarboxypeptidase"
                     /db_xref="GeneID:5547"
                     /db_xref="HGNC:HGNC:9344"
                     /db_xref="MIM:176785"
     exon            1..52
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            53..154
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1234
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /EC_number="3.4.16.2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     angiotensinase C; lysosomal Pro-x carboxypeptidase;
                     proline carboxypeptidase; lysosomal carboxypeptidase C"
                     /codon_start=1
                     /product="lysosomal Pro-X carboxypeptidase isoform 3"
                     /protein_id="NP_001306143.1"
                     /db_xref="CCDS:CCDS91553.1"
                     /db_xref="GeneID:5547"
                     /db_xref="HGNC:HGNC:9344"
                     /db_xref="MIM:176785"
                     /translation="MWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQ
                     ALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIW
                     QFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCS
                     PLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLL
                     LQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFE
                     PHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTK
                     DITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH"
     exon            155..336
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            337..494
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            495..664
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            665..829
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1017
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..3204
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1750..1755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAAA"
     polyA_site      1773
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="major polyA site"
     regulatory      3002..3007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAGA"
     polyA_site      3022
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
     regulatory      3170..3175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
                     /note="hexamer: AATAAA"
     polyA_site      3204
                     /gene="PRCP"
                     /gene_synonym="HUMPCP; PCP"
ORIGIN      
        1 agcgaggaaa tcctacaagc atctggaaac gaccattttc gtcaaactgc gggggttcat
       61 gtgggatgtg gctgaggaac tgaaagctat gttggtgttt gctgaacatc gatactatgg
      121 agagtctctc ccctttggtg acaactcatt caaggattcc agacacttga atttcctgac
      181 atcagaacaa gctctggctg attttgcaga gttaatcaaa cacttgaaaa gaacaatccc
      241 aggagctgaa aatcaacctg tcattgccat aggaggctcc tatggtggca tgcttgccgc
      301 ctggtttagg atgaaatatc ctcatatggt agttggagct cttgcagctt ctgcccctat
      361 ctggcagttt gaggatttag taccttgtgg tgtatttatg aagatcgtaa ctacagattt
      421 taggaaaagc ggtccacatt gttcagagag catccacagg tcctgggatg ccattaatcg
      481 actctcaaat actggcagtg gtttgcagtg gcttactgga gcccttcact tatgcagccc
      541 attaacttct caggacatcc aacatttgaa agactggatc tctgaaacct gggtgaatct
      601 ggcaatggtg gactatcctt atgcctctaa ctttttacag cctttgcctg cttggcctat
      661 caaggtagtg tgccagtatt tgaaaaatcc caatgtatct gattcactgc tgctgcagaa
      721 tattttccaa gctctgaatg tatattacaa ttattcgggc caggtgaaat gcctgaatat
      781 ttcagagaca gcaactagca gtctgggaac actgggttgg agctatcagg cctgcacaga
      841 agtagtcatg cccttttgta ctaatggtgt cgatgacatg tttgaacctc actcatggaa
      901 cttaaaggaa ctttctgatg actgttttca acagtggggt gtgagaccaa ggccctcctg
      961 gatcactact atgtatggag gcaaaaacat tagttcacac acaaacattg ttttcagcaa
     1021 tggtgaacta gacccctggt caggaggtgg agtaactaag gatatcacag acactctggt
     1081 tgcagtcacc atctcagagg gggcccacca cttagatctc cgcaccaaga atgccttgga
     1141 tcctatgtct gtgctgttag cccgctcctt ggaagttaga catatgaaga attggatcag
     1201 agatttctat gacagtgcgg gaaagcagca ctgagaaact tttgattgtt ttcaatttct
     1261 tcttttatgt tcacaccacc acattcccat tcactttgat tttctacatg taattacctt
     1321 cttttgttta tcattagatt tgatggggcc aaagttgaga tagaatagag ggtgatgacg
     1381 gtaagagcaa gtgtcccatg aatgtgattt cctgggttct cactgtcttt gcaccacgtc
     1441 taggaagaat cttcttgata gctctcccac accatcagtg gccctcataa ctggagtaga
     1501 gttcctggtt gcttttcata agagggagag ttactttctt tgtatctctg caagcagaga
     1561 tttctctttg gttttgaggt tgaagtgtct ttggcccatt tgtaagtccc catccctacc
     1621 ctacacaaag taaaagcaga agatagataa aaaatgatgt aattgcagct ggtaggatgt
     1681 ctggtgccaa tccaggaagt gagagccatt tcttttgtac cggatttaat gactttgaac
     1741 tgtgctgtaa ataaataata cagctggacc ttatacctgt gtgttgcttc tgaagctcac
     1801 ttcaactccg gctgatggaa atgctgtgtg cactgggctt agctgttttt ggaaagtcct
     1861 ttaatttttt ctcttccact tggcagcttt tcttagttct gggatgttgg ccactgtggt
     1921 actgccagta agaaatctgg cctttttctg ctccatttct ctttgtttct aaggtcagtt
     1981 ttaatttgtt tgaattctgc cagtttagga aaatgactct gttttgtctt tttaaatctg
     2041 agcaagcttt atgctgtagt cactaattat atttcagtaa gaagccttta aaaattgatg
     2101 aatcagctat tccaaaagta gaatatagta atattcagct tttaaatttt taaaatttta
     2161 ttttctgata gttgttactg ctgtcattaa aaccagaagt gttagacact aacaggcagg
     2221 tgcacaagaa cttttctgta tgacttccaa aagacaagct gactaattca tgcaggcagc
     2281 atatttgaca ttaataaatg gccaagccat tcctatttcc aaggggttgg cttctttcaa
     2341 gcagttctta cactgagacc tttcttcaga gagcttttct aggataacaa atagggttgg
     2401 atcaaaacct ttcagggaca cagagagaca gagagagaga gactaacagt ttctactctg
     2461 ttgaaagatt tcttcctcct tgtttctttt cattttctct caataatttt tttttcttca
     2521 ccctgccttg ggaacatttt ctgcttttaa aaaactgtac cttgagcttg atcattttta
     2581 tggcattttt tttttttaag ggcatatata gagttcattc ttgggatgga aagctgaaat
     2641 atttccttta ctttccctgg catctgggag atacaggaaa atctttgtct tattttggga
     2701 gccttcagtt ctcattgtaa tgagttgtct atcaaagcat ttggtaacta ttttgtttca
     2761 gcatcacaga acaatcgatt tgggtgggaa tttaagggcc aaaacaatac tagtttttat
     2821 tctttgtcaa ctaagaaatt aaataatgca gggtttatgg gctcaataga ggatattctt
     2881 acaatttatt ttacgatttt agggtttttc ccttgtgtag aatctttaaa atactttgcc
     2941 attttatttg atgttgatta tttggatgag ttacattatt ctgaagaaac aaactgcaaa
     3001 aaatagaatt ttttggcaag cataattgta tcctattact gtatgcattt taggaatata
     3061 gatagcagcc ttaattgaag tgagtataat tataatatgc taaggtttaa ttttaacatg
     3121 aaaaagaatt ggagtgtcag ataagcctta ttatttaaag catccctcta ataaaactgc
     3181 actttttact gacagggaaa gaga
//
