LOCUS       NM_003981               3072 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens protein regulator of cytokinesis 1 (PRC1), transcript
            variant 1, mRNA.
ACCESSION   NM_003981
VERSION     NM_003981.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ciapponi,M., Karlukova,E., Schkolziger,S., Benda,C. and Muller,J.
  TITLE     Structural basis of the histone ubiquitination read-write mechanism
            of RYBP-PRC1
  JOURNAL   Nat Struct Mol Biol 31 (7), 1023-1027 (2024)
   PUBMED   38528151
  REMARK    GeneRIF: Structural basis of the histone ubiquitination read-write
            mechanism of RYBP-PRC1.
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Gluszek-Kustusz,A., Craske,B., Legal,T., McHugh,T. and Welburn,J.P.
  TITLE     Phosphorylation controls spatial and temporal activities of
            motor-PRC1 complexes to complete mitosis
  JOURNAL   EMBO J 42 (21), e113647 (2023)
   PUBMED   37592895
  REMARK    GeneRIF: Phosphorylation controls spatial and temporal activities
            of motor-PRC1 complexes to complete mitosis.
REFERENCE   3  (bases 1 to 3072)
  AUTHORS   Walia,G.K., Sharma,P., Agarwal,T., Lal,M., Negandhi,H.,
            Prabhakaran,D., Khadgawat,R., Sachdeva,M.P. and Gupta,V.
  TITLE     Genetic associations of TMEM154, PRC1 and ZFAND6 loci with type 2
            diabetes in an endogamous business community of North India
  JOURNAL   PLoS One 18 (9), e0291339 (2023)
   PUBMED   37738238
  REMARK    GeneRIF: Genetic associations of TMEM154, PRC1 and ZFAND6 loci with
            type 2 diabetes in an endogamous business community of North India.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3072)
  AUTHORS   Li,S., Motino,O., Lambertucci,F., Martins,I., Sun,L. and Kroemer,G.
  TITLE     Protein regulator of cytokinesis 1: a potential oncogenic driver
  JOURNAL   Mol Cancer 22 (1), 128 (2023)
   PUBMED   37563591
  REMARK    GeneRIF: Protein regulator of cytokinesis 1: a potential oncogenic
            driver.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3072)
  AUTHORS   Yi,M., Lv,S., He,Y., Wu,Y., Zhang,J. and Chen,Q.
  TITLE     CircKDM1B promotes hepatocellular carcinoma progression through
            regulating miR-1322/PRC1 axis
  JOURNAL   Environ Toxicol 38 (8), 2031-2040 (2023)
   PUBMED   37227723
  REMARK    GeneRIF: CircKDM1B promotes hepatocellular carcinoma progression
            through regulating miR-1322/PRC1 axis.
REFERENCE   6  (bases 1 to 3072)
  AUTHORS   Kurasawa,Y., Earnshaw,W.C., Mochizuki,Y., Dohmae,N. and Todokoro,K.
  TITLE     Essential roles of KIF4 and its binding partner PRC1 in organized
            central spindle midzone formation
  JOURNAL   EMBO J 23 (16), 3237-3248 (2004)
   PUBMED   15297875
  REMARK    GeneRIF: kinesin family member 4 and its binding partner PRC1 play
            essential roles in the organization of central spindles and midzone
            formation
REFERENCE   7  (bases 1 to 3072)
  AUTHORS   Ban,R., Irino,Y., Fukami,K. and Tanaka,H.
  TITLE     Human mitotic spindle-associated protein PRC1 inhibits MgcRacGAP
            activity toward Cdc42 during the metaphase
  JOURNAL   J Biol Chem 279 (16), 16394-16402 (2004)
   PUBMED   14744859
  REMARK    GeneRIF: contributes to the correct formation of the spindle during
            the metaphase
REFERENCE   8  (bases 1 to 3072)
  AUTHORS   Li,C., Shridhar,K. and Liu,J.
  TITLE     Molecular characterization of oncostatin M-induced growth arrest of
            MCF-7 cells expressing a temperature-sensitive mutant of p53
  JOURNAL   Breast Cancer Res Treat 80 (1), 23-37 (2003)
   PUBMED   12889596
REFERENCE   9  (bases 1 to 3072)
  AUTHORS   Mollinari,C., Kleman,J.P., Jiang,W., Schoehn,G., Hunter,T. and
            Margolis,R.L.
  TITLE     PRC1 is a microtubule binding and bundling protein essential to
            maintain the mitotic spindle midzone
  JOURNAL   J Cell Biol 157 (7), 1175-1186 (2002)
   PUBMED   12082078
  REMARK    GeneRIF: PRC1 is a microtubule-associated protein required to
            maintain the spindle midzone, and that distinct functions are
            associated with modular elements of the primary sequence.
REFERENCE   10 (bases 1 to 3072)
  AUTHORS   Jiang,W., Jimenez,G., Wells,N.J., Hope,T.J., Wahl,G.M., Hunter,T.
            and Fukunaga,R.
  TITLE     PRC1: a human mitotic spindle-associated CDK substrate protein
            required for cytokinesis
  JOURNAL   Mol Cell 2 (6), 877-885 (1998)
   PUBMED   9885575
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068831.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 24, 2018 this sequence version replaced NM_003981.3.
            
            Summary: This gene encodes a protein that is involved in
            cytokinesis. The protein is present at high levels during the S and
            G2/M phases of mitosis but its levels drop dramatically when the
            cell exits mitosis and enters the G1 phase. It is located in the
            nucleus during interphase, becomes associated with mitotic spindles
            in a highly dynamic manner during mitosis, and localizes to the
            cell mid-body during cytokinesis. This protein has been shown to be
            a substrate of several cyclin-dependent kinases (CDKs). It is
            necessary for polarizing parallel microtubules and concentrating
            the factors responsible for contractile ring assembly. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jun 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003138.1, AF044588.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394249.8/ ENSP00000377793.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               AC068831.17        83246-83374         c
            130-262             AC068831.17        73532-73664         c
            263-385             AC068831.17        72857-72979         c
            386-619             AC068831.17        70587-70820         c
            620-790             AC068831.17        70338-70508         c
            791-940             AC068831.17        69723-69872         c
            941-1088            AC068831.17        69081-69228         c
            1089-1225           AC068831.17        67997-68133         c
            1226-1321           AC068831.17        65515-65610         c
            1322-1468           AC068831.17        63424-63570         c
            1469-1579           AC068831.17        62975-63085         c
            1580-1690           AC068831.17        59243-59353         c
            1691-1867           AC068831.17        58286-58462         c
            1868-1909           AC068831.17        57918-57959         c
            1910-3072           AC068831.17        54879-56041         c
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..3072
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /note="protein regulator of cytokinesis 1"
                     /db_xref="GeneID:9055"
                     /db_xref="HGNC:HGNC:9341"
                     /db_xref="MIM:603484"
     exon            1..129
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..1981
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein regulating cytokinesis 1; anaphase spindle
                     elongation 1 homolog"
                     /codon_start=1
                     /product="protein regulator of cytokinesis 1 isoform 1"
                     /protein_id="NP_003972.2"
                     /db_xref="CCDS:CCDS32334.1"
                     /db_xref="GeneID:9055"
                     /db_xref="HGNC:HGNC:9341"
                     /db_xref="MIM:603484"
                     /translation="MRRSEVLAEESIVCLQKALNHLREIWELIGIPEDQRLQRTEVVK
                     KHIKELLDMMIAEEESLKERLIKSISVCQKELNTLCSELHVEPFQEEGETTILQLEKD
                     LRTQVELMRKQKKERKQELKLLQEQDQELCEILCMPHYDIDSASVPSLEELNQFRQHV
                     TTLRETKASRREEFVSIKRQIILCMEALDHTPDTSFERDVVCEDEDAFCLSLENIATL
                     QKLLRQLEMQKSQNEAVCEGLRTQIRELWDRLQIPEEEREAVATIMSGSKAKVRKALQ
                     LEVDRLEELKMQNMKKVIEAIRVELVQYWDQCFYSQEQRQAFAPFCAEDYTESLLQLH
                     DAEIVRLKNYYEVHKELFEGVQKWEETWRLFLEFERKASDPNRFTNRGGNLLKEEKQR
                     AKLQKMLPKLEEELKARIELWEQEHSKAFMVNGQKFMEYVAEQWEMHRLEKERAKQER
                     QLKNKKQTETEMLYGSAPRTPSKRRGLAPNTPGKARKLNTTTMSNATANSSIRPIFGG
                     TVYHSPVSRLPPSGSKPVAASTCSGKKTPRTGRHGANKENLELNGSILSGGYPGSAPL
                     QRNFSINSVASTYSEFAKDPSLSDSSTVGLQRELSKASKSDATSGILNSTNIQS"
     exon            130..262
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            263..385
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            386..619
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            620..790
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            791..940
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1088
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1225
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1321
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1468
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1579
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1690
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1867
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1909
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..3072
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3043..3048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /note="hexamer: ATTAAA"
     polyA_site      3072
                     /gene="PRC1"
                     /gene_synonym="ASE1; MAP65"
                     /note="major polyA site"
ORIGIN      
        1 aacggctcgc ggagcggcta cgcggagtga catcgccggt gtttgcgggt ggttgttgct
       61 ctcggggccg tgtggagtag gtctggacct ggactcacgg ctgcttggag cgtccgccat
      121 gaggagaagt gaggtgctgg cggaggagtc catagtatgt ctgcagaaag ccctaaatca
      181 ccttcgggaa atatgggagc taattgggat tccagaggac cagcggttac aaagaactga
      241 ggtggtaaag aagcatatca aggaactcct ggatatgatg attgctgaag aggaaagcct
      301 gaaggaaaga ctcatcaaaa gcatatccgt ctgtcagaaa gagctgaaca ctctgtgcag
      361 cgagttacat gttgagccat ttcaggaaga aggagagacg accatcttgc aactagaaaa
      421 agatttgcgc acccaagtgg aattgatgcg aaaacagaaa aaggagagaa aacaggaact
      481 gaagctactt caagagcaag atcaagaact gtgcgaaatt ctttgtatgc cccactatga
      541 tattgacagt gcctcagtgc ccagcttaga agagctgaac cagttcaggc aacatgtgac
      601 aactttgagg gaaacaaagg cttctaggcg tgaggagttt gtcagtataa agagacagat
      661 catactgtgt atggaagcat tagaccacac cccagacaca agctttgaaa gagatgtggt
      721 gtgtgaagac gaagatgcct tttgtttgtc tttggagaat attgcaacac tacaaaagtt
      781 gctacggcag ctggaaatgc agaaatcaca aaatgaagca gtgtgtgagg ggctgcgtac
      841 tcaaatccga gagctctggg acaggttgca aatacctgaa gaagaaagag aagctgtggc
      901 caccattatg tctgggtcaa aggccaaggt ccggaaagcg ctgcaattag aagtggatcg
      961 gttggaagaa ctgaaaatgc aaaacatgaa gaaagtgatt gaggcaattc gagtggagct
     1021 ggttcagtac tgggaccagt gcttttatag ccaggagcag agacaagctt ttgccccttt
     1081 ctgtgctgag gactacacag aaagtctgct ccagctccac gatgctgaga ttgtgcggtt
     1141 aaaaaactac tatgaagttc acaaggaact ctttgaaggt gtccagaagt gggaagaaac
     1201 ctggaggctt ttcttagagt ttgagagaaa agcttcagat ccaaatcgat ttacaaaccg
     1261 aggaggaaat cttctaaaag aagaaaaaca acgagccaag ctccagaaaa tgctgcccaa
     1321 gctggaagaa gagttgaagg cacgaattga attgtgggaa caggaacatt caaaggcatt
     1381 tatggtgaat gggcagaaat tcatggagta tgtggcagaa caatgggaga tgcatcgatt
     1441 ggagaaagag agagccaagc aggaaagaca actgaagaac aaaaaacaga cagagacaga
     1501 gatgctgtat ggcagcgctc ctcgaacacc tagcaagcgg cgaggactgg ctcccaatac
     1561 accgggcaaa gcacgtaagc tgaacactac caccatgtcc aatgctacgg ccaatagtag
     1621 cattcggcct atctttggag ggacagtcta ccactccccc gtgtctcgac ttcctccttc
     1681 tggcagcaag ccagtcgctg cttccacctg ttcagggaag aaaacacccc gtactggcag
     1741 gcatggagcc aacaaggaga acctggagct caacggcagc atcctgagtg gtgggtaccc
     1801 tggctcggcc cccctccagc gcaacttcag cattaattct gttgccagca cctattctga
     1861 gtttgcgaag gatccgtccc tctctgacag ttccactgtt gggcttcagc gagaactttc
     1921 aaaggcttcc aaatctgatg ctacttctgg aatcctcaat tcaaccaaca tccagtcctg
     1981 agaagccctg atcagtcaac cagctgtggc ttcctgtgcc tagactggac ctaattatat
     2041 gggggtgact ttagtttttc ttcagcttag gcgtgcttga aaccttggcc aggttccatg
     2101 accatgggcc taacttaaag atgtgaatga gtgttacagt tgaaagccca tcataggttt
     2161 agtggtccta ggagacttgg ttttgactta tatacatgaa aagtttatgg caagaagtgc
     2221 aaattttagc atatggggcc tgacttctct accacataat tctacttgct gaagcatgat
     2281 caaagcttgt tttatttcac cactgtagga aaatgattga ctatgcccat ccctgggggt
     2341 aattttggca tgtatacctg taactagtaa ttaacatctt ttttgtttag gcatgttcaa
     2401 ttaatgctgt agctatcata gctttgctct tacctgaagc cttgtcccca ccacacagga
     2461 cagccttcct cctgaagaga atgtctttgt gtgtccgaag ttgagatggc ctgccctact
     2521 gccaaagagg tgacaggaag gctgggagca gctttgttaa attgtgttca gttctgttac
     2581 acagtgcatt gccctttgtt gggggtatgc atgtatgaac acacatgctt gtcggaacgc
     2641 tttctcggcg tttgtccctt ggctctcatc tcccccattc ctgtgcctac tttgcctgag
     2701 ttcttctacc cccgcagttg ccagccacat tgggagtctg tttgttccaa tgggttgagc
     2761 tgtctttgtc gtggagatct ggaactttgc acatgtcact actggggagg tgttcctgct
     2821 ctagcttcca cgatgaggcg ccctctttac ctatcctctc aatcactact cttcttgaag
     2881 cactattatt tattcttccg ctgtctgcct gcagcagtac tactgtcaac atagtgtaaa
     2941 tggttctcaa aagcttacca gtgtggactt ggtgttagcc acgctgttta ctcatacagt
     3001 acgtgtcctg tttttaaaat atacaattat tcttaaaaat aaattaaaat ctgtatactt
     3061 acatttcaaa aa
//
