LOCUS       NM_002723                923 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens proline rich protein BstNI subfamily 4 (PRB4),
            transcript variant 1, mRNA.
ACCESSION   NM_002723
VERSION     NM_002723.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Manconi,B., Castagnola,M., Cabras,T., Olianas,A., Vitali,A.,
            Desiderio,C., Sanna,M.T. and Messana,I.
  TITLE     The intriguing heterogeneity of human salivary proline-rich
            proteins: Short title: Salivary proline-rich protein species
  JOURNAL   J Proteomics 134, 47-56 (2016)
   PUBMED   26375204
REFERENCE   2  (bases 1 to 923)
  AUTHORS   Castagnola,M., Cabras,T., Iavarone,F., Vincenzoni,F., Vitali,A.,
            Pisano,E., Nemolato,S., Scarano,E., Fiorita,A., Vento,G.,
            Tirone,C., Romagnoli,C., Cordaro,M., Paludetti,G., Faa,G. and
            Messana,I.
  TITLE     Top-down platform for deciphering the human salivary proteome
  JOURNAL   J Matern Fetal Neonatal Med 25 (Suppl 5), 27-43 (2012)
   PUBMED   23025766
  REMARK    Review article
REFERENCE   3  (bases 1 to 923)
  AUTHORS   Vitorino,R., Alves,R., Barros,A., Caseiro,A., Ferreira,R.,
            Lobo,M.C., Bastos,A., Duarte,J., Carvalho,D., Santos,L.L. and
            Amado,F.L.
  TITLE     Finding new posttranslational modifications in salivary
            proline-rich proteins
  JOURNAL   Proteomics 10 (20), 3732-3742 (2010)
   PUBMED   20879038
REFERENCE   4  (bases 1 to 923)
  AUTHORS   Helmerhorst,E.J., Sun,X., Salih,E. and Oppenheim,F.G.
  TITLE     Identification of Lys-Pro-Gln as a novel cleavage site specificity
            of saliva-associated proteases
  JOURNAL   J Biol Chem 283 (29), 19957-19966 (2008)
   PUBMED   18463091
REFERENCE   5  (bases 1 to 923)
  AUTHORS   Kauffman,D.L., Keller,P.J., Bennick,A. and Blum,M.
  TITLE     Alignment of amino acid and DNA sequences of human proline-rich
            proteins
  JOURNAL   Crit Rev Oral Biol Med 4 (3-4), 287-292 (1993)
   PUBMED   8373986
REFERENCE   6  (bases 1 to 923)
  AUTHORS   Kauffman,D.L., Bennick,A., Blum,M. and Keller,P.J.
  TITLE     Basic proline-rich proteins from human parotid saliva:
            relationships of the covalent structures of ten proteins from a
            single individual
  JOURNAL   Biochemistry 30 (14), 3351-3356 (1991)
   PUBMED   1849422
REFERENCE   7  (bases 1 to 923)
  AUTHORS   Lyons,K.M., Azen,E.A., Goodman,P.A. and Smithies,O.
  TITLE     Many protein products from a few loci: assignment of human salivary
            proline-rich proteins to specific loci
  JOURNAL   Genetics 120 (1), 255-265 (1988)
   PUBMED   3220251
REFERENCE   8  (bases 1 to 923)
  AUTHORS   Lyons,K.M., Stein,J.H. and Smithies,O.
  TITLE     Length polymorphisms in human proline-rich protein genes generated
            by intragenic unequal crossing over
  JOURNAL   Genetics 120 (1), 267-278 (1988)
   PUBMED   2851479
REFERENCE   9  (bases 1 to 923)
  AUTHORS   Azen,E.A. and Maeda,N.
  TITLE     Molecular genetics of human salivary proteins and their
            polymorphisms
  JOURNAL   Adv Hum Genet 17, 141-199 (1988)
   PUBMED   3055850
  REMARK    Review article
REFERENCE   10 (bases 1 to 923)
  AUTHORS   Maeda,N., Kim,H.S., Azen,E.A. and Smithies,O.
  TITLE     Differential RNA splicing and post-translational cleavages in the
            human salivary proline-rich protein gene system
  JOURNAL   J Biol Chem 260 (20), 11123-11130 (1985)
   PUBMED   2993301
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010176.12.
            
            On Aug 19, 2019 this sequence version replaced NM_002723.5.
            
            Summary: This gene encodes a member of the heterogeneous family of
            basic, proline-rich, human salivary glycoproteins. The encoded
            preproprotein undergoes proteolytic processing to generate one or
            more mature peptides before secretion from the parotid glands.
            Multiple alleles of this gene exhibiting variations in the length
            of the tandem repeats have been identified. The reference genome
            encodes the 'Small' allele. This gene is located in a cluster of
            closely related salivary proline-rich proteins on chromosome 12.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms that may undergo similar proteolytic
            processing. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279838.2498.1, ERR279871.3102.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2150585 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000279575.7/ ENSP00000279575.1
            RefSeq Select criteria :: based on manual assertion, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               AC010176.12        66951-67052
            103-138             AC010176.12        67982-68017
            139-800             AC010176.12        68505-69166
            801-923             AC010176.12        70188-70310
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.2"
     gene            1..923
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /note="proline rich protein BstNI subfamily 4"
                     /db_xref="GeneID:5545"
                     /db_xref="HGNC:HGNC:9340"
                     /db_xref="MIM:180990"
     exon            1..102
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..782
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; BstNI type basic salivary proline-rich protein
                     4; parotid o protein; salivary proline-rich protein Po;
                     salivary proline-rich protein II-1"
                     /codon_start=1
                     /product="basic salivary proline-rich protein 4 isoform 1
                     preproprotein"
                     /protein_id="NP_002714.2"
                     /db_xref="CCDS:CCDS8641.1"
                     /db_xref="GeneID:5545"
                     /db_xref="HGNC:HGNC:9340"
                     /db_xref="MIM:180990"
                     /translation="MLLILLSVALLALSSAESSSEDVSQEESLFLISGKPEGRRPQGG
                     NQPQRPPPPPGKPQGPPPQGGNQSQGPPPPPGKPEGRPPQGGNQSQGPPPHPGKPERP
                     PPQGGNQSQGTPPPPGKPERPPPQGGNQSHRPPPPPGKPERPPPQGGNQSQGPPPHPG
                     KPEGPPPQEGNKSRSARSPPGKPQGPPQQEGNKPQGPPPPGKPQGPPPAGGNPQQPQA
                     PPAGKPQGPPPPPQGGRPPRPAQGQQPPQ"
     exon            103..138
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /inference="alignment:Splign:2.1.0"
     exon            139..800
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /inference="alignment:Splign:2.1.0"
     exon            801..923
                     /gene="PRB4"
                     /gene_synonym="Po"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcacagagt tgggagtgac tccagagcct ccagcgagat gctgctgatt ctgctgtcag
       61 tggccctgct ggccctgagc tcagctgaga gttcaagtga agatgtcagc caggaagaat
      121 ctctcttcct aatatcagga aagccagaag gacgacgccc acaaggagga aaccagcccc
      181 aacgtccccc acctcctcca ggaaagccac aaggaccacc cccacaagga ggaaaccagt
      241 cccaaggtcc cccacctcct ccaggaaagc cagaaggacg acccccacaa ggaggcaacc
      301 agtcccaagg tcccccacct catccaggaa agccagaaag accaccccca caaggaggaa
      361 accagtccca aggtacccca cctcctccag gaaagccaga aagaccaccc ccacaaggag
      421 gcaaccagtc ccaccgtccc ccacctcctc caggaaagcc agaaagacca cccccacaag
      481 gaggtaacca gtcccaaggt cccccacctc atccaggaaa gccagaagga ccacccccac
      541 aggaaggaaa caagtcccga agtgcccgat ctcctccagg aaagccacaa ggaccacccc
      601 aacaagaagg caacaagcct caaggtcccc cacctcctgg aaagccacaa ggcccacccc
      661 cagcaggagg caatccccag cagcctcagg cacctcctgc tggaaagccc caggggccac
      721 ctccacctcc tcaagggggc aggccaccca gacctgccca gggacaacag cctccccagt
      781 aatctaggat tcaatgacag gaagtgaata agaagatatc agtgaattca aataattcaa
      841 ttgctacaaa tgccgtgaca ttggaacaag gtcatcatag ctctaacttt aatataccaa
      901 taaaataatc agcttgcaat ttc
//
