LOCUS       NM_145202                744 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens proline rich acidic protein 1 (PRAP1), transcript
            variant 1, mRNA.
ACCESSION   NM_145202 XM_370588
VERSION     NM_145202.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 744)
  AUTHORS   Wolfarth,A.A., Liu,X., Darby,T.M., Boyer,D.J., Spizman,J.B.,
            Owens,J.A., Chandrasekharan,B., Naudin,C.R., Hanley,K.Z.,
            Robinson,B.S., Ortlund,E.A., Jones,R.M. and Neish,A.S.
  TITLE     Proline-Rich Acidic Protein 1 (PRAP1) Protects the Gastrointestinal
            Epithelium From Irradiation-Induced Apoptosis
  JOURNAL   Cell Mol Gastroenterol Hepatol 10 (4), 713-727 (2020)
   PUBMED   32629119
  REMARK    GeneRIF: Proline-Rich Acidic Protein 1 (PRAP1) Protects the
            Gastrointestinal Epithelium From Irradiation-Induced Apoptosis.
REFERENCE   3  (bases 1 to 744)
  AUTHORS   Li,M., Zhai,G., Gu,X. and Sun,K.
  TITLE     ATF3 and PRAP1 play important roles in cisplatin-induced damages in
            microvascular endothelial cells
  JOURNAL   Gene 672, 93-105 (2018)
   PUBMED   29886035
  REMARK    GeneRIF: ATF3 and PRAP1 play important roles in cisplatin-induced
            DNA damage repair process.
REFERENCE   4  (bases 1 to 744)
  AUTHORS   Sze,K.M., Chu,G.K., Mak,Q.H., Lee,J.M. and Ng,I.O.
  TITLE     Proline-rich acidic protein 1 (PRAP1) is a novel interacting
            partner of MAD1 and has a suppressive role in mitotic checkpoint
            signalling in hepatocellular carcinoma
  JOURNAL   J Pathol 233 (1), 51-60 (2014)
   PUBMED   24374861
  REMARK    GeneRIF: PRAP1 is a protein interacting partner of MAD1 and that
            PRAP1 is able to down-regulate MAD1 and suppress mitotic checkpoint
            signalling in hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 744)
  AUTHORS   Huang,B.H., Zhuo,J.L., Leung,C.H., Lu,G.D., Liu,J.J., Yap,C.T. and
            Hooi,S.C.
  TITLE     PRAP1 is a novel executor of p53-dependent mechanisms in cell
            survival after DNA damage
  JOURNAL   Cell Death Dis 3 (12), e442 (2012)
   PUBMED   23235459
  REMARK    GeneRIF: Induction of PRAP1 expression by p53 in response to
            DNA-damaging agents contributes to cancer cell survival.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 744)
  AUTHORS   Diao,H., Xiao,S., Zhao,F. and Ye,X.
  TITLE     Uterine luminal epithelium-specific proline-rich acidic protein 1
            (PRAP1) as a marker for successful embryo implantation
  JOURNAL   Fertil Steril 94 (7), 2808-11 (2010)
   PUBMED   20674898
REFERENCE   7  (bases 1 to 744)
  AUTHORS   Li,J., Liu,F., Wang,H., Liu,X., Liu,J., Li,N., Wan,F., Wang,W.,
            Zhang,C., Jin,S., Liu,J., Zhu,P. and Liu,Y.
  TITLE     Systematic mapping and functional analysis of a family of human
            epididymal secretory sperm-located proteins
  JOURNAL   Mol Cell Proteomics 9 (11), 2517-2528 (2010)
   PUBMED   20736409
REFERENCE   8  (bases 1 to 744)
  AUTHORS   Zhang,J., Wong,H., Ramanan,S., Cheong,D., Leong,A. and Hooi,S.C.
  TITLE     The proline-rich acidic protein is epigenetically regulated and
            inhibits growth of cancer cell lines
  JOURNAL   Cancer Res 63 (20), 6658-6665 (2003)
   PUBMED   14583459
REFERENCE   9  (bases 1 to 744)
  AUTHORS   Kasik,J. and Rice,E.
  TITLE     A novel complementary deoxyribonucleic acid is abundantly and
            specifically expressed in the uterus during pregnancy
  JOURNAL   Am J Obstet Gynecol 176 (2), 452-456 (1997)
   PUBMED   9065197
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL360181.37 and BC071872.1.
            
            On Nov 22, 2018 this sequence version replaced NM_145202.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.14338.1, AY358908.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000433452.6/ ENSP00000416126.2
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AL360181.37        54886-54919
            35-346              BC071872.1         1-312
            347-357             AL360181.37        59799-59809
            358-744             BC071872.1         324-710
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.3"
     gene            1..744
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="proline rich acidic protein 1"
                     /db_xref="GeneID:118471"
                     /db_xref="HGNC:HGNC:23304"
                     /db_xref="MIM:609776"
     exon            1..53
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..501
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; epididymis tissue protein Li 178; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="proline-rich acidic protein 1 isoform 1
                     precursor"
                     /protein_id="NP_660203.3"
                     /db_xref="CCDS:CCDS7679.1"
                     /db_xref="GeneID:118471"
                     /db_xref="HGNC:HGNC:23304"
                     /db_xref="MIM:609776"
                     /translation="MRRLLLVTSLVVVLLWEAGAVPAPKVPIKMQVKHWPSEQDPEKA
                     WGARVVEPPEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGHV
                     LSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ"
     exon            54..120
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /inference="alignment:Splign:2.1.0"
     exon            121..173
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /inference="alignment:Splign:2.1.0"
     exon            174..307
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /inference="alignment:Splign:2.1.0"
     exon            308..744
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      589..594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="hexamer: AATAAA"
     polyA_site      609
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="major polyA site"
     regulatory      720..725
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="hexamer: ACTAAA"
     regulatory      726..731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
                     /note="hexamer: AATACA"
     polyA_site      738
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
     polyA_site      744
                     /gene="PRAP1"
                     /gene_synonym="PRO1195; UPA"
ORIGIN      
        1 actgcagctc cctgagcact ctctacagag acgcggaccc cagacatgag gaggctcctc
       61 ctggtcacca gcctggtggt tgtgctgctg tgggaggcag gtgcagtccc agcacccaag
      121 gtccctatca agatgcaagt caaacactgg ccctcagagc aggacccaga gaaggcctgg
      181 ggcgcccgtg tggtggagcc tccggagaag gacgaccagc tggtggtgct gttccctgtc
      241 cagaagccga aactcttgac caccgaggag aagccacgag gtcagggcag gggccccatc
      301 cttccaggca ccaaggcctg gatggagacc gaggacaccc tgggccatgt cctgagtccc
      361 gagcccgacc atgacagcct gtaccaccct ccgcctgagg aggaccaggg cgaggagagg
      421 ccccggttgt gggtgatgcc aaatcaccag gtgctcctgg gaccggagga agaccaagac
      481 cacatctacc acccccagta gggctccagg ggccatcact gcccccgccc tgtcccaagg
      541 cccaggctgt tgggactggg accctcccta ccctgcccca gctagacaaa taaaccccag
      601 caggccgggc gcggtggctc acctctgtaa tcccagcact tttagaggcc gaggcaggcg
      661 gatcacctga aatcaggagt tccagaccag cctgggcaac atggtgaaac cccgtctcta
      721 ctaaaaatac aaaaattagc cggg
//
