LOCUS       NM_001080826            4845 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens PEAK1 related, kinase-activating pseudokinase 1
            (PRAG1), transcript variant 1, mRNA.
ACCESSION   NM_001080826 XM_005272369 XM_291277 XM_937796
VERSION     NM_001080826.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Buianova,A.A., Proskura,M.V., Cheranev,V.V., Belova,V.A.,
            Shmitko,A.O., Pavlova,A.S., Vasiliadis,I.A., Suchalko,O.N.,
            Rebrikov,D.V., Petrosyan,E.K. and Korostin,D.O.
  TITLE     Candidate Genes for IgA Nephropathy in Pediatric Patients:
            Exome-Wide Association Study
  JOURNAL   Int J Mol Sci 24 (21), 15984 (2023)
   PUBMED   37958966
  REMARK    GeneRIF: Candidate Genes for IgA Nephropathy in Pediatric Patients:
            Exome-Wide Association Study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 4845)
  AUTHORS   Safari,F., Shafiee Nejad,N. and Aghaei Nejad,A.
  TITLE     The inhibition of Panc1 cancer cells invasion by hAMSCs secretome
            through suppression of tyrosine phosphorylation of SGK223 (at Y411
            site), c-Src (at Y416, Y530 sites), AKT activity, and JAK1/Stat3
            signaling
  JOURNAL   Med Oncol 39 (3), 28 (2022)
   PUBMED   35059869
  REMARK    GeneRIF: The inhibition of Panc1 cancer cells invasion by hAMSCs
            secretome through suppression of tyrosine phosphorylation of SGK223
            (at Y411 site), c-Src (at Y416, Y530 sites), AKT activity, and
            JAK1/Stat3 signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4845)
  AUTHORS   Lecointre,C., Simon,V., Kerneur,C., Allemand,F., Fournet,A.,
            Montarras,I., Pons,J.L., Gelin,M., Brignatz,C., Urbach,S.,
            Labesse,G. and Roche,S.
  TITLE     Dimerization of the Pragmin Pseudo-Kinase Regulates Protein
            Tyrosine Phosphorylation
  JOURNAL   Structure 26 (4), 545-554 (2018)
   PUBMED   29503074
  REMARK    GeneRIF: It was discovered that pseudo-kinase pragmin uses the
            tyrosine kinase CSK to induce protein tyrosine phosphorylation in
            human cells.
            Erratum:[Structure. 2018 Nov 6;26(11):1563. doi:
            10.1016/j.str.2018.10.020.. PMID: 30403993]
REFERENCE   5  (bases 1 to 4845)
  AUTHORS   Patel,O., Griffin,M.D.W., Panjikar,S., Dai,W., Ma,X., Chan,H.,
            Zheng,C., Kropp,A., Murphy,J.M., Daly,R.J. and Lucet,I.S.
  TITLE     Structure of SgK223 pseudokinase reveals novel mechanisms of
            homotypic and heterotypic association
  JOURNAL   Nat Commun 8 (1), 1157 (2017)
   PUBMED   29079850
  REMARK    GeneRIF: analysis of how the kinase fold mediates non-catalytic
            functions in SgK223
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4845)
  AUTHORS   Safari,F., Murata-Kamiya,N., Saito,Y. and Hatakeyama,M.
  TITLE     Mammalian Pragmin regulates Src family kinases via the
            Glu-Pro-Ile-Tyr-Ala (EPIYA) motif that is exploited by bacterial
            effectors
  JOURNAL   Proc Natl Acad Sci U S A 108 (36), 14938-14943 (2011)
   PUBMED   21873224
  REMARK    GeneRIF: Pragmin as a mammalian EPIYA effector and suggests that
            bacterial EPIYA effectors target Pragmin to subvert SFKs for
            successful infection.
REFERENCE   7  (bases 1 to 4845)
  AUTHORS   Tanaka,H., Katoh,H. and Negishi,M.
  TITLE     Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity
  JOURNAL   J Biol Chem 281 (15), 10355-10364 (2006)
   PUBMED   16481321
  REMARK    GeneRIF: Rnd2 regulates neurite outgrowth by functioning as the
            RhoA activator through Pragmin, in contrast to Rnd1 and Rnd3
            inhibiting RhoA signaling
REFERENCE   8  (bases 1 to 4845)
  AUTHORS   Zhang,Y., Wolf-Yadlin,A., Ross,P.L., Pappin,D.J., Rush,J.,
            Lauffenburger,D.A. and White,F.M.
  TITLE     Time-resolved mass spectrometry of tyrosine phosphorylation sites
            in the epidermal growth factor receptor signaling network reveals
            dynamic modules
  JOURNAL   Mol Cell Proteomics 4 (9), 1240-1250 (2005)
   PUBMED   15951569
REFERENCE   9  (bases 1 to 4845)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   10 (bases 1 to 4845)
  AUTHORS   Manning,G., Whyte,D.B., Martinez,R., Hunter,T. and Sudarsanam,S.
  TITLE     The protein kinase complement of the human genome
  JOURNAL   Science 298 (5600), 1912-1934 (2002)
   PUBMED   12471243
  REMARK    GeneRIF: Also known as SgK223, a member of the NKF3 subfamily of
            eukaryotic protein kinases.
            Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC103957.8, BX420505.2, AK122582.1, AC068353.34, ABBA01026926.1 and
            AL833872.1.
            
            On or before Apr 17, 2019 this sequence version replaced
            XM_005272369.5, NM_001080826.2.
            
            Summary: This gene encodes an enzyme that belongs to the tyrosine
            protein kinase family. A similar protein in rat binds to Rho family
            GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of
            Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar
            2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: The coding region has been updated to add two internal
            6-nt sequences that were included in the update of the GRCh37
            reference genome assembly to the GRCh38 assembly. The update
            results in a protein that is 4 aa longer.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.956320.1,
                                           SRR11853564.25398.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000615670.5/ ENSP00000481109.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AC103957.8         103014-103132
            120-274             BX420505.2         175-329
            275-1416            AK122582.1         1-1142
            1417-2054           AC103957.8         112249-112886
            2055-3542           AK122582.1         1781-3268
            3543-3704           AC068353.34        57099-57260         c
            3705-3719           ABBA01026926.1     23264-23278
            3720-4510           AC068353.34        56299-57089         c
            4511-4837           AK122582.1         4238-4564
            4838-4845           AL833872.1         2175-2182
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.1"
     gene            1..4845
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /note="PEAK1 related, kinase-activating pseudokinase 1"
                     /db_xref="GeneID:157285"
                     /db_xref="HGNC:HGNC:25438"
                     /db_xref="MIM:617344"
     exon            1..119
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     exon            120..536
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..4427
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /EC_number="2.7.10.2"
                     /note="tyrosine-protein kinase SgK223; sugen kinase 223;
                     homolog of rat pragma of Rnd2; Pragmin, RND2 effector
                     protein; tyrosine-protein kinase PRAG1; PEAK family member
                     2"
                     /codon_start=1
                     /product="inactive tyrosine-protein kinase PRAG1"
                     /protein_id="NP_001074295.2"
                     /db_xref="CCDS:CCDS43706.1"
                     /db_xref="GeneID:157285"
                     /db_xref="HGNC:HGNC:25438"
                     /db_xref="MIM:617344"
                     /translation="MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVA
                     GPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEA
                     NLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYT
                     MVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTT
                     SGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSR
                     GRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEA
                     AISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLG
                     LTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNA
                     WAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQ
                     NSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPP
                     VSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQR
                     RPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSG
                     QNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGS
                     AESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPS
                     GSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNV
                     HDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKG
                     APSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCED
                     LFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPE
                     PKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCV
                     VVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDL
                     CLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGP
                     REKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIY
                     ELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGE
                     AKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVE
                     LEDWLCCQYLASAEPGALLQSLKLLQLL"
     exon            537..2368
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2526
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..3278
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..4845
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4818..4823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /note="hexamer: ATTAAA"
     polyA_site      4845
                     /gene="PRAG1"
                     /gene_synonym="NACK; PEAK2; PRAGMIN; SGK223"
                     /note="major polyA site"
ORIGIN      
        1 agaagccacc cgcccgcggc cgggcacgca ccgcgcgcag accttctgcg aacaatgctc
       61 cggccgggcg gctggcggct cggagaccga cggaggggcc gggggagcgc agcccagagg
      121 aagccgctct gtgacccacc tctgaatccc acaaaactgc accagagagc cgggcgcaag
      181 atgaaccagc accctgtcgg ctcaagatgc accagaccct ctgcctgaac cccgagagcc
      241 tgaaaatgtc tgcgtgcagt gactttgtgg agcacatctg gaaacccggg tcctgcaaga
      301 actgcttctg cctgcggagc gaccaccagc tggtggccgg ccctccccag cccagagcgg
      361 gcagcctgcc ccctccaccg cgcctgcctc ccaggcctga gaactgccgc ctggaagatg
      421 aaggtgtgaa cagctcacct tactccaagc ccacaattgc cgtgaagccc accatgatga
      481 gctccgaggc ctctgatgtg tggacagagg ccaacctgag tgccgaagtc tcgcaggtca
      541 tctggagacg agcccctggc aagctccccc tcccgaagca ggaggatgcc cccgtcgtct
      601 acctgggcag cttccgaggt gtacagaagc ctgctggtcc ctctacctcc cctgatggca
      661 attctcgctg tcccccagct tacaccatgg tcggcctgca caaccttgag ccccgcggcg
      721 agaggaacat tgccttccac ccggtgagct tcccggagga gaaggctgtg cacaaagaaa
      781 aaccctcatt tccttaccaa gaccggccct ccacccagga gagcttccgc cagaaactgg
      841 ctgcctttgc tgggaccaca tctggctgtc accagggccc tgggcccctg cgggaatccc
      901 tgccctcgga ggatgacagt gatcaaaggt gctcgccctc cggggacagc gagggtggag
      961 agtactgctc catcctggac tgctgccctg ggagccctgt tgccaaggct gcctcccaga
     1021 ctgcaggttc ccggggcagg catggtggca gggactgctc acccacgtgc tgggagcagg
     1081 ggaagtgttc cgggcccgca gagcaggaga agcggggccc gagcttcccc aaggagtgct
     1141 gtagccaggg ccccactgcc cacccatcct gcctgggccc caagaaactg tccctcacct
     1201 cggaggctgc catttcttcc gacggcctct cttgtggcag cggcagcggc agcggcagcg
     1261 gcgccagtag ccccttcgtc ccccacctcg agagtgatta ctgctccctc atgaaggaac
     1321 ctgccccaga gaagcagcag gaccctggct gcccaggggt gacccctagc agatgccttg
     1381 ggctgacggg ggagccccag cccccggccc acccccggga ggctacacag cctgaaccca
     1441 tctatgctga gagcaccaag aggaagaagg cagctccggt gccttccaag tcacaggcca
     1501 agatagaaca tgcagctgct gcccagggcc aaggccaggt atgcacaggt aatgcctggg
     1561 cccagaaagc agcatctggc tggggccggg acagcccaga cccaactccc caggtgtcag
     1621 ccaccatcac agtcatggcg gcccacccgg aagaggacca tcggacgatc tacctgagca
     1681 gccctgactc tgcagtgggg gtgcagtggc cacgagggcc tgtgagccag aactccgagg
     1741 taggtgaaga ggagacttcg gctgggcagg ggctgagctc cagggaaagc catgctcaca
     1801 gtgccagcga gagcaagccc aaggagaggc ccgccattcc ccccaagttg tccaagagta
     1861 gccctgtagg gtccccggtg tcaccgtctg ctggagggcc cccagtgtca ccgctggctg
     1921 accttagtga tgggagctct ggcggcagca gcattgggcc ccagcctcca tcccaaggtc
     1981 ctgctgaccc cgctccttcc tgccggacca acggtgtcgc tatcagtgac ccatccaggt
     2041 gtccccagcc tgccgcctcg tcagcctcgg aacagaggcg gcccaggttc caggcaggca
     2101 cctggagtcg tcagtgccgg atagaggaag aagaggaggt ggagcaggaa ttgctgagtc
     2161 acagctgggg aagagagacc aaaaatggcc ccacggacca ttcaaactcc acgacctggc
     2221 accgtctcca ccccacagat ggctcctctg ggcagaacag caaagttggg accgggatga
     2281 gcaaatccgc ctcttttgcc tttgagttcc ccaaggacag aagtgggatt gagacattct
     2341 cacctcctcc tccgcctcca aagtcgcggc accttctaaa aatgaacaag agcagctctg
     2401 atttggaaaa agtgagccag ggctctgcag aaagcctcag cccatccttc aggggtgtcc
     2461 acgtcagctt caccaccggc tccacggaca gcctggcctc agactctagg acctgcagcg
     2521 atggaggtcc ctcgtctgag ctggctcact cgcccaccaa cagcgggaag aagctctttg
     2581 ctcccgttcc gtttccttca ggctccactg aggacgtgtc ccccagtggc ccccagcagc
     2641 cccctccact cccccagaaa aagatagtga gccgggcagc ctcttcaccg gatggcttct
     2701 tctggaccca aggctccccc aagcccggaa cagcaagccc caagctgaac ctaagccact
     2761 cggaaaccaa cgtccacgac gaatctcact ttagctattc gttgagcccc gggaaccgcc
     2821 accatcctgt cttctcctct tccgatcctc tggagaaagc tttcaaaggc agtggccact
     2881 ggcttccggc agcagggctg gcgggcaaca gaggcggctg cgggagccct ggcctccagt
     2941 gcaaaggggc cccctccgcc tcatcctccc agctgagcgt gtccagtcaa gcctccaccg
     3001 ggagcaccca gcttcagctg cacggtctcc tgagcaacat cagcagcaag gagggcacct
     3061 atgccaagct ggggggactc tacacccagt ccctggcccg ccttgtagcc aaatgtgagg
     3121 acctcttcat gggcggccag aaaaaggagc tccacttcaa tgagaataac tggtcgctct
     3181 tcaagctgac ttgtaacaag ccctgctgtg actcggggga tgccatttat tactgtgcca
     3241 cctgctctga ggaccccggc agcacctatg ctgtgaaaat ctgcaaagcc cctgagccca
     3301 aaacagtctc ctactgcagc ccgtccgtgc ccgtgcactt taacatccag caggactgcg
     3361 gccacttcgt cgcctcggtg ccgtccagca tgctcagctc ccccgacgcg cccaaggacc
     3421 ctgtgcctgc cctgcccaca cacccccctg cccaggagca ggactgcgtg gtggtcatca
     3481 cccgagaggt gccacatcag accgcctccg acttcgtgcg ggactcggcg gccagccacc
     3541 aggcggagcc cgaggcgtac gagcggcgcg tgtgcttcct gcttctgcaa ctctgcaacg
     3601 ggctggagca cctgaaggag cacgggatca tccaccggga cctgtgcctg gagaacctgc
     3661 tgctggtgca ctgcaccctc caggccggcc ccgggcccgc ccccgccccc gccccggctc
     3721 ccgcccccgc cgccgccgcg cctccctgct cctctgccgc cccgcctgct ggtggcactc
     3781 tcagccccgc agccggcccc gcctccccgg aagggccccg ggagaagcag ctgccccggc
     3841 tcatcatcag caactttttg aaggccaagc agaagccggg cggcacccca aacctgcagc
     3901 agaagaagag ccaggcccgg ctggcccccg agatcgtgtc tgcttcccag taccgcaagt
     3961 tcgatgagtt ccagacaggc atcctcatct acgagctgct gcaccaaccc aacccgttcg
     4021 aggtgcgcgc ccagctgcgg gagagagact accggcagga ggacctgccg ccgctgcccg
     4081 cgctgtccct ctactcaccc ggcctgcagc agctggcaca tctgctactg gaggccgacc
     4141 ccatcaagcg tatccgcatc ggcgaggcca agcgcgtgct gcagtgcctg ctgtgggggc
     4201 ctcggcgcga gctggtgcag cagccgggca cctcggagga ggcgctgtgc ggcacgctgc
     4261 acaactggat cgacatgaag cgggccctga tgatgatgaa gtttgcggag aaggcggtgg
     4321 atcgcaggcg gggcgtggag ctggaggact ggctttgctg ccagtacctg gcgtctgcgg
     4381 agcccggggc cctcttacag tcgctgaagc tcctgcagct tctgtgagcc aagccccagc
     4441 ctgcaccgtc gctgcccctt ccctgcctaa ccctttcctg tctcgccttg gaagcaccca
     4501 tgtctccctg ggaaatggta cagatgactg ggatacctgg atgtaaaata tataaatata
     4561 tatatagaaa atacatatac catatataaa tatgaaagac taaggatgct gttgcccgtc
     4621 cacactcgtc tcctctctgc actaagtcct ccctgttttc ttctgtaatt atacacattt
     4681 ccagttccat gcaacgtcct gaggacagtt ctgtgaactg aatgcagcct ggacactggc
     4741 ctcaatacct tgtttaggat ttcttcaccc ttttgtcaaa ttgttattta aagaaaaaaa
     4801 aaaaagaaga aaattccatt aaaatttttt ttggttgagt catga
//
