LOCUS       NM_001164161            5072 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 6 regulatory subunit 3 (PPP6R3),
            transcript variant 6, mRNA.
ACCESSION   NM_001164161
VERSION     NM_001164161.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5072)
  AUTHORS   Heo,J., Larner,J.M. and Brautigan,D.L.
  TITLE     Protein kinase CK2 phosphorylation of SAPS3 subunit increases PP6
            phosphatase activity with Aurora A kinase
  JOURNAL   Biochem J 477 (2), 431-444 (2020)
   PUBMED   31904830
  REMARK    GeneRIF: The substitution of Ala in nine putative phosphorylation
            sites in SAPS3 was required to prevent CK2 activation of the
            phosphatase. Different CK2 chemical inhibitors equally increased
            phosphorylation of endogenous AURKA in living cells, consistent
            with reduction in PP6 activity.
REFERENCE   4  (bases 1 to 5072)
  AUTHORS   Smebye,M.L., Agostini,A., Johannessen,B., Thorsen,J., Davidson,B.,
            Trope,C.G., Heim,S., Skotheim,R.I. and Micci,F.
  TITLE     Involvement of DPP9 in gene fusions in serous ovarian carcinoma
  JOURNAL   BMC Cancer 17 (1), 642 (2017)
   PUBMED   28893231
  REMARK    GeneRIF: we found a DPP9-PPP6R3 fusion transcript in one tumor
            showing a matching genomic 11;19-translocation. Another tumor had a
            rearrangement of DPP9 with PLIN3. Both rearrangements were
            associated with diminished expression of the 3' end of DPP9
            corresponding to the breakpoints identified by RNA-seq.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5072)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   6  (bases 1 to 5072)
  AUTHORS   Nasu-Nishimura,Y., Hayashi,T., Ohishi,T., Okabe,T., Ohwada,S.,
            Hasegawa,Y., Senda,T., Toyoshima,C., Nakamura,T. and Akiyama,T.
  TITLE     Role of the Rho GTPase-activating protein RICS in neurite outgrowth
  JOURNAL   Genes Cells 11 (6), 607-614 (2006)
   PUBMED   16716191
REFERENCE   7  (bases 1 to 5072)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 5072)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   9  (bases 1 to 5072)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   10 (bases 1 to 5072)
  AUTHORS   Twells,R.C., Metzker,M.L., Brown,S.D., Cox,R., Garey,C.,
            Hammond,H., Hey,P.J., Levy,E., Nakagawa,Y., Philips,M.S., Todd,J.A.
            and Hess,J.F.
  TITLE     The sequence and gene characterization of a 400-kb candidate region
            for IDDM4 on chromosome 11q13
  JOURNAL   Genomics 72 (3), 231-242 (2001)
   PUBMED   11401438
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP314824.1, CR933658.1, AB046778.2 and BC007738.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001164161.1.
            
            Summary: Protein phosphatase regulatory subunits, such as SAPS3,
            modulate the activity of protein phosphatase catalytic subunits by
            restricting substrate specificity, recruiting substrates, and
            determining the intracellular localization of the holoenzyme. SAPS3
            is a regulatory subunit for the protein phosphatase-6 catalytic
            subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 [PubMed
            16769727]).[supplied by OMIM, Nov 2010].
            
            Transcript Variant: This variant (6) includes an additional exon in
            the 5' UTR, lacks three alternate in-frame exons, and uses an
            alternate splice site resulting in a distinct 3' coding region,
            compared to variant 1. The resulting isoform (6) lacks three
            internal segments and has a distinct C-terminus, compared to
            isoform 1. Variants 6, 28, and 35 all encode the same isoform (6).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR936718.1, SRR1803612.50796.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393800.7/ ENSP00000377389.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 BP314824.1         35-37
            4-1662              CR933658.1         1-1659
            1663-2263           AB046778.2         1645-2245
            2264-3653           CR933658.1         2261-3650
            3654-5072           BC007738.2         1476-2894
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..5072
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="protein phosphatase 6 regulatory subunit 3"
                     /db_xref="GeneID:55291"
                     /db_xref="HGNC:HGNC:1173"
                     /db_xref="MIM:610879"
     exon            1..76
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            77..227
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            228..460
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..2855
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="isoform 6 is encoded by transcript variant 6; SAPS
                     domain family, member 3; serine/threonine-protein
                     phosphatase 6 regulatory subunit 3; sporulation-induced
                     transcript 4-associated protein SAPL"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 6
                     regulatory subunit 3 isoform 6"
                     /protein_id="NP_001157633.1"
                     /db_xref="CCDS:CCDS53672.1"
                     /db_xref="GeneID:55291"
                     /db_xref="HGNC:HGNC:1173"
                     /db_xref="MIM:610879"
                     /translation="MFWKFDLHSSSHIDTLLEREDVTLKELMDEEDVLQECKAQNRKL
                     IEFLLKAECLEDLVSFIIEEPPQDMDEKIRYKYPNISCELLTSDVSQMNDRLGEDESL
                     LMKLYSFLLNDSPLNPLLASFFSKVLSILISRKPEQIVDFLKKKHDFVDLIIKHIGTS
                     AIMDLLLRLLTCIEPPQPRQDVLNWLNEEKIIQRLVEIVHPSQEEDRHSNASQSLCEI
                     VRLSRDQMLQIQNSTEPDPLLATLEKQEIIEQLLSNIFHKEKNESAIVSAIQILLTLL
                     ETRRPTFEGHIEICPPGMSHSACSVNKSVLEAIRGRLGSFHELLLEPPKKSVMKTTWG
                     VLDPPVGNTRLNVIRLISSLLQTNTSSINGDLMELNSIGVILNMFFKYTWNNFLHTQV
                     EICIALILASPFENTENATITDQDSTGDNLLLKHLFQKCQLIERILEAWEMNEKKQAE
                     GGRRHGYMGHLTRIANCIVHSTDKGPNSALVQQLIKDLPDEVRERWETFCTSSLGETN
                     KRNTVDLVTTCHIHSSSDDEIDFKETGFSQDSSLQQAFSDYQMQQMTSNFIDQFGFND
                     EKFADQDDIGNVSFDRVSDINFTLNTNESGNIALFEACCKERIQQFDDGGSDEEDIWE
                     EKHIAFTPESQRRSSSGSTDSEESTDSEEEDGAKQDLFEPSSANTEDKMEVDLSEPPN
                     WSANFDVPMETTHGAPLDSVGSDVWSTEEPMPTKETGWASFSEFTSSLSTKDSLRSNS
                     PVEMETSTEPMDPLTPSAAALAVQPEAAGSVAMEASSDGEEDAESTDKVTETVMNGGM
                     KETLSLTVDAKTETAVFKSEEGKLSTSQDAACKDAEECPETAEAKCAAPRPPSSSPEQ
                     RTGQPSAPGDTSVNGPV"
     exon            461..647
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            648..785
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            786..851
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            852..964
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1078
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1208
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1361
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1511
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1576
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1692
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1778
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1865
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1963
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2018
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2149
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2271
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2425
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2532
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2683
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2803
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..5072
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4312..4317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="hexamer: AATAAA"
     polyA_site      4330
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="major polyA site"
     regulatory      5044..5049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="hexamer: AATAAA"
     polyA_site      5072
                     /gene="PPP6R3"
                     /gene_synonym="C11orf23; PP6R3; SAP190; SAPL; SAPLa;
                     SAPS3"
                     /note="major polyA site"
ORIGIN      
        1 gggcgcttcg ctgatggtgt cggtgagcgc gtttcccgcc tgagcgcaac tagcggcggg
       61 tcgtgggcac ctccaggaga gcttgtttca tatccatatc ccactgtatt cctgctaatc
      121 tgctaatgca gtaaattgga ggaaaactgt taccaggata acctgtaatg ggcaaggagc
      181 cacaaagaag aaaacatttc ttttaatttt taaacttggt ttgaaagacc agcatgtttt
      241 ggaaatttga tcttcactca tcatcccaca tagacacact tctagaaaga gaagatgtaa
      301 cactgaagga gttaatggat gaggaagatg ttttacagga atgtaaagct cagaaccgca
      361 aacttataga gtttctgtta aaagcagaat gtctcgaaga tttagtctca ttcattatag
      421 aagaaccacc tcaagacatg gatgaaaaga tcagatacaa gtatccaaat atatcttgtg
      481 agttgctcac ttctgatgtc tcccagatga atgatagact gggagaagat gaatccttgc
      541 taatgaaatt atatagcttc ctcctaaacg attccccttt gaatccacta cttgccagtt
      601 tcttcagcaa ggtgctaagt attcttatca gcagaaaacc agaacagatt gtggatttct
      661 taaagaagaa gcatgatttt gtagacctta ttataaagca cataggaact tctgctatca
      721 tggatttgtt gctcaggctc ctgacgtgta tcgaacctcc acagcccagg caagatgtgc
      781 tgaattggtt aaatgaggag aaaattatcc agaggcttgt ggaaatagtt catccatcgc
      841 aagaagaaga tcgacattca aatgcatcac aatcactttg tgaaattgtt cgcctgagca
      901 gagaccagat gttacaaatt cagaacagta cagagcccga ccccctgctt gccactctag
      961 aaaagcaaga aattatagag cagcttctat caaatatttt ccacaaggag aaaaatgagt
     1021 cagccatagt cagtgcaatc cagatattgc tgactttact tgagacacga cgaccaacat
     1081 ttgaaggcca tatagagatc tgcccaccag gcatgagcca ttcagcttgt tcagtaaaca
     1141 agagtgttct agaagccatc agaggaagac ttggatcttt tcatgaactc ctgctggagc
     1201 cacccaagaa aagtgtgatg aagaccacat ggggtgtgct ggatcctcct gtggggaata
     1261 cccggttgaa tgtcattagg ttgatatcca gcctgcttca aaccaatacc agcagtataa
     1321 atggggacct tatggagctg aatagcattg gagtcatatt gaacatgttc ttcaagtata
     1381 catggaataa ctttttgcat acacaagtgg aaatttgtat tgcactgatt cttgcaagtc
     1441 cttttgaaaa cacagaaaat gccacaatta ccgatcaaga ctccactggt gataatttgt
     1501 tattaaaaca tcttttccaa aaatgtcaat taatagaacg aatacttgaa gcctgggaaa
     1561 tgaatgagaa gaaacaggct gagggaggaa gacggcatgg ttacatggga cacctaacga
     1621 ggatagctaa ctgtatcgtg cacagcactg acaagggccc caacagtgca ttagtgcagc
     1681 agcttatcaa agatcttccc gacgaagtca gggaacgatg ggagacgttc tgcacaagct
     1741 ccttaggaga aactaacaag aggaacacgg tagatctagt tacaacctgc catattcatt
     1801 catccagtga tgatgaaatt gactttaaag aaacgggttt ctcacaggat tcttctttgc
     1861 agcaagcctt ttctgattat cagatgcaac aaatgacgtc caattttatt gaccagtttg
     1921 gcttcaacga tgagaagttt gcagatcaag atgacattgg caatgtttct tttgatcgag
     1981 tatcagacat caactttact ctcaatacaa atgaaagtgg aaatattgcc ttgtttgaag
     2041 catgttgtaa ggaaagaata caacagtttg atgatggtgg ctctgatgag gaagatatat
     2101 gggaggaaaa gcacatcgca ttcacaccag aatcccaaag acgatccagc tcggggagta
     2161 cagacagtga ggaaagtaca gactctgaag aagaagatgg agcaaagcaa gacttgtttg
     2221 aacccagcag tgccaacacg gaggataaaa tggaggtgga cctgagtgaa ccacccaact
     2281 ggtcagctaa ctttgatgtc ccaatggaaa caacccacgg tgctccattg gattctgtgg
     2341 gatctgatgt ctggagcaca gaggagccga tgccaactaa agagacgggc tgggcttctt
     2401 tttcagagtt cacgtcttcc ctgagcacaa aagattcttt aaggagtaat tctccagtgg
     2461 aaatggaaac cagcactgaa cccatggacc ctctgactcc cagtgcggct gccctggcag
     2521 tgcagccaga agcggcaggc agtgtggcca tggaagccag ctctgacgga gaggaggatg
     2581 cagaaagtac agacaaggta actgagacag tgatgaatgg cggcatgaag gaaacgctca
     2641 gcctcactgt agatgccaag acagagactg cggtcttcaa aagtgaggaa gggaaactgt
     2701 ctacctctca agatgctgct tgtaaagacg cagaggagtg tcccgagact gcagaggcga
     2761 agtgcgcggc gcccaggcct cccagcagca gtcccgagca gaggactggc caaccaagcg
     2821 caccaggtga cacttcagtg aatggccctg tatgacgggt gacgtctgct gctgctgact
     2881 gaggactgca gaccgccacc actcaggggc tctggagggg tcagctggag cccaccaagc
     2941 tgtcactgct gcactcactc tgcaagggat caggaccagc aacctttata ttctagattc
     3001 taagacattg tacagagaaa ttcagaagtg taaaaatatt gcacattgac aaataccaag
     3061 aatttttgcg tatgtttata ttgtattgtt ctaaataatg ggtagcctgt gaaataagat
     3121 cttgccaccc atgtaataat agtagtaata ctatagttaa aatggctgta agaatagttt
     3181 tataaaagtg aatacacaga tctattgtat ttgaaacata actttgacaa ttattagtgt
     3241 gaccaaagta ttaggcggtt ttcatacatt tttcaccttg tacaaaatta tgaattcatt
     3301 tttcctccag gccgacaagg agttgtagaa tgaaaatgcc ctctaagtgt tattttggtt
     3361 gttctaactt acaaaagtga ttttgaataa gaaatatttg gtgttctttt tataaccagt
     3421 ttttgattgg taattgtttt ctgtattgtt taaaacggat caaaaatgta agtctattgg
     3481 tagagattaa gtaaagtatt tattgctaca tcatagttga taaattgatg ttatcgtaaa
     3541 gccatatgtt ctgttcaagt cttgtttgct tgaaatgatt attcctacaa gtgaaacact
     3601 agactatttg gagtgtatat ggcttgtgtt ttgggatttt tttttttttt ttttggcttt
     3661 tgtttttgtt tgtttttttg tttcatttgg tagttcatct gccttttaac ccattcacca
     3721 aaatttacct tgttaacaag catcaccaat gaacatttca gagcaatctg catatttaac
     3781 agacctaaaa taaatcctat taggcaagtc agttgaaaat gctcgtgctg ctaatggaat
     3841 tagagtgcgt tcattttaca ggctagtatt ttaaaagtag aaatcaaaat ctggcaccga
     3901 agcatgctaa ttgtttactg taccttgtga ggttttcact cataaattta aaccagtgta
     3961 tttttttaga actggtttgt gtatatatat agtgattatg gatactaatt caatgtaatt
     4021 tataattttc tatgtcaata caaaaataca tcacagcctt ctcaaacagc tcaagcaata
     4081 tattgtatat tgccatatcg tctggtgaaa gggttaaatt acttcacctc ttgcactttt
     4141 agatgcaaat cagtttttca tttctgtaat agaaaattat tcacgtattt ttacatcatt
     4201 tgtttttcct gaccagtatt taaaaccaaa aggatattct gaaaaatggc caacaatttt
     4261 tttagaagta gcatcccaag cagcgtgcct aaacattaca ttgcatatgg aaataaaaga
     4321 atcaaacgtc taatgcctta ttatttctga tttccttttt cattttaagt ggtgtggaga
     4381 ttccagcact cccaggacag tggagtcagc agtaagccct gggacaggtg gcaagggtgg
     4441 gtcccttgac ctttgcacgc ctcctcagga accccctttc ccgggtgagc ccctctctga
     4501 agagactgtc cttgggcctc ctctggaagc agcaccccca gaggacaggg ctcctcctgc
     4561 ttgcctcagg gctgcctgac ttgaatggcg ttggacctcg gggattactg gtagataata
     4621 tgctctggtc tcgcctggtg gtgagttttg ccagccatgg ccagggtttg gctccactgg
     4681 tggcacacgt ggcctccgtg gtatggacct ggtggcttct ccatcccact gtggcctctg
     4741 tggtatggac ctggtggctt ctccatccta cccaaggtaa cagtgtcttg cttcatccca
     4801 ctgactgctg ggagagagcc tctgggactt ttctttgggg catcattttg ttttgtcttt
     4861 cgtagcaggg aaaggatatg acaatgggga ggacagttct tttggaggtt ggaggggcca
     4921 agccaaggac aggagcaagt gtgccctcat tttgtttcta cttttaattt ctgtgtgttg
     4981 gccatactga attatgagac taacagatgt ctacaataca atacctgtat tcaaaataac
     5041 aaaaataaag cctgattctt tgtttctaga aa
//
