LOCUS       NM_001242898            4094 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 6 regulatory subunit 2 (PPP6R2),
            transcript variant 1, mRNA.
ACCESSION   NM_001242898
VERSION     NM_001242898.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4094)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 4094)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   5  (bases 1 to 4094)
  AUTHORS   Couzens,A.L., Knight,J.D., Kean,M.J., Teo,G., Weiss,A.,
            Dunham,W.H., Lin,Z.Y., Bagshaw,R.D., Sicheri,F., Pawson,T.,
            Wrana,J.L., Choi,H. and Gingras,A.C.
  TITLE     Protein interaction network of the mammalian Hippo pathway reveals
            mechanisms of kinase-phosphatase interactions
  JOURNAL   Sci Signal 6 (302), rs15 (2013)
   PUBMED   24255178
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4094)
  AUTHORS   Marquez,J., Kohli,M., Arteta,B., Chang,S., Li,W.B., Goldblatt,M.
            and Vidal-Vanaclocha,F.
  TITLE     Identification of hepatic microvascular adhesion-related genes of
            human colon cancer cells using random homozygous gene perturbation
  JOURNAL   Int J Cancer 133 (9), 2113-2122 (2013)
   PUBMED   23629598
REFERENCE   7  (bases 1 to 4094)
  AUTHORS   Zeng,K., Bastos,R.N., Barr,F.A. and Gruneberg,U.
  TITLE     Protein phosphatase 6 regulates mitotic spindle formation by
            controlling the T-loop phosphorylation state of Aurora A bound to
            its activator TPX2
  JOURNAL   J Cell Biol 191 (7), 1315-1332 (2010)
   PUBMED   21187329
REFERENCE   8  (bases 1 to 4094)
  AUTHORS   Stefansson,B., Ohama,T., Daugherty,A.E. and Brautigan,D.L.
  TITLE     Protein phosphatase 6 regulatory subunits composed of ankyrin
            repeat domains
  JOURNAL   Biochemistry 47 (5), 1442-1451 (2008)
   PUBMED   18186651
REFERENCE   9  (bases 1 to 4094)
  AUTHORS   Stefansson,B. and Brautigan,D.L.
  TITLE     Protein phosphatase 6 subunit with conserved Sit4-associated
            protein domain targets IkappaBepsilon
  JOURNAL   J Biol Chem 281 (32), 22624-22634 (2006)
   PUBMED   16769727
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX545851.6, DB452735.1,
            AK302472.1, AL671545.13 and AL096767.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001242898.1.
            
            Summary: The protein encoded by this gene is a regulatory protein
            for the protein phosphatase-6 catalytic subunit. Together, these
            proteins act as a significant T-loop phosphatase for Aurora A, an
            essential mitotic kinase. Loss of function of either the regulatory
            or catalytic subunit of protein phosphatase-6 interferes with
            spindle formation and chromosome alignment. [provided by RefSeq,
            May 2017].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.272956.1,
                                           SRR3476690.990363.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000612753.5/ ENSP00000478417.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                BX545851.6         1819-1839
            22-313              DB452735.1         32-323
            314-1041            AK302472.1         235-962
            1042-1042           AL671545.13        19896-19896
            1043-2765           AK302472.1         964-2686
            2766-2766           AL096767.14        16271-16271
            2767-3268           AK302472.1         2688-3189
            3269-4094           AL096767.14        19694-20519
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.33"
     gene            1..4094
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="protein phosphatase 6 regulatory subunit 2"
                     /db_xref="GeneID:9701"
                     /db_xref="HGNC:HGNC:19253"
                     /db_xref="MIM:610877"
     exon            1..224
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            225..355
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            356..598
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             372..3251
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="isoform 1 is encoded by transcript variant 1; SAPS
                     domain family, member 2; serine/threonine-protein
                     phosphatase 6 regulatory subunit 2"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 6
                     regulatory subunit 2 isoform 1"
                     /protein_id="NP_001229827.1"
                     /db_xref="CCDS:CCDS74881.1"
                     /db_xref="GeneID:9701"
                     /db_xref="HGNC:HGNC:19253"
                     /db_xref="MIM:610877"
                     /translation="MFWKFDLNTTSHVDKLLDKEHVTLQELMDEDDILQECKAQNQKL
                     LDFLCRQQCMEELVSLITQDPPLDMEEKVRFKYPNTACELLTCDVPQISDRLGGDESL
                     LSLLYDFLDHEPPLNPLLASFFSKTIGNLIARKTEQVITFLKKKDKFISLVLKHIGTS
                     ALMDLLLRLVSCVEPAGLRQDVLHWLNEEKVIQRLVELIHPSQDEDRQSNASQTLCDI
                     VRLGRDQGSQLQEALEPDPLLTALESQDCVEQLLKNMFDGDRTESCLVSGTQVLLTLL
                     ETRRVGTEGLVDSFSQGLERSYAVSSSVLHGIEPRLKDFHQLLLNPPKKKAILTTIGV
                     LEEPLGNARLHGARLMAALLHTNTPSINQELCRLNTMDLLLDLFFKYTWNNFLHFQVE
                     LCIAAILSHAAREERTEASGSESRVEPPHENGNRSLETPQPAASLPDNTMVTHLFQKC
                     CLVQRILEAWEANDHTQAAGGMRRGNMGHLTRIANAVVQNLERGPVQTHISEVIRGLP
                     ADCRGRWESFVEETLTETNRRNTVDLVSTHHLHSSSEDEDIEGAFPNELSLQQAFSDY
                     QIQQMTANFVDQFGFNDEEFADQDDNINAPFDRIAEINFNIDADEDSPSAALFEACCS
                     DRIQPFDDDEDEDIWEDSDTRCAARVMARPRFGAPHASESCSKNGPERGGQDGKASLE
                     AHRDAPGAGAPPAPGKKEAPPVEGDSEGAMWTAVFDEPANSTPTAPGVVRDVGSSVWA
                     AGTSAPEEKGWAKFTDFQPFCCSESGPRCSSPVDTECSHAEGSRSQGPEKAFSPASPC
                     AWNVCVTRKAPLLASDSSSSGGSHSEDGDQKAASAMDAVSRGPGREAPPLPTVARTEE
                     AVGRVGCADSRLLSPACPAPKEVTAAPAVAVPPEATVAITTALSKAGPAIPTPAVSSA
                     LAVAVPLGPIMAVTAAPAMVATLGTVTKDGKTDAPPEGAALNGPV"
     exon            599..785
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            786..923
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            924..989
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1102
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1216
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1343
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1496
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1706
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1771
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1887
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..1973
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2054
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2152
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2210
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2335
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2336..2499
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2656
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2745
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2746..2950
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3202
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3203..4094
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3358..3363
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="hexamer: AATAAA"
     polyA_site      3395
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="major polyA site"
     regulatory      4069..4074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="hexamer: AATAAA"
     polyA_site      4094
                     /gene="PPP6R2"
                     /gene_synonym="KIAA0685; PP6R2; SAP190; SAPS2"
                     /note="major polyA site"
ORIGIN      
        1 gccgccattt tggagcgatc ggagtgccgc ccgcggcccc gagtcggtct cgagccgccg
       61 gccggccgtg ccggtgtccg taggcgctgc gccctcggcc gggcccatgt gtgtgcggcc
      121 cgcccgaggc cgcccgggct ttgcctccac cagcgccctg gcctccgctc gggcctccac
      181 acgggcctcc gaagagctgc cgcgacgccc ggcccgcagg gcaggtaaag agattataaa
      241 tcttccactg aatgaaaaaa attttcttaa agctgcatat actccaagaa aaaaaccaca
      301 aatgtttttc tgttttgcct gaatacatga tttaaacaag agatttccac agaagctctg
      361 cggccgtcac gatgttctgg aagtttgact tgaacaccac gtcccatgtt gacaagctgc
      421 tggacaagga gcatgtgacg ctgcaggagt taatggatga agatgacatc ttgcaggagt
      481 gtaaggctca gaaccagaag ctgctggact tcctgtgcag gcagcagtgc atggaggagc
      541 tggtgagcct catcacacag gatccgcccc tggacatgga ggagaaggtc cgcttcaaat
      601 atccaaacac agcctgtgag cttctgactt gtgatgtgcc gcagatcagc gaccgcctcg
      661 gtggggacga gagcctgctg agcctcctgt acgacttctt ggaccatgag ccgcctctca
      721 atcctctgct cgccagtttt ttcagcaaga ccattggcaa tctcattgca agaaaaaccg
      781 aacaggtgat tacgtttttg aagaagaagg acaagttcat cagcctggtg ttgaagcaca
      841 tcggcacctc agcgcttatg gacctgctgc tgcgcctggt cagctgtgtg gagccagccg
      901 ggctccggca ggacgtcctg cactggctga atgaagagaa ggtcatccag aggcttgtgg
      961 agttgatcca cccgagccag gatgaagata ggcagtcaaa tgcttctcag actctctgtg
     1021 acatagttag gctgggcaga gaccagggca gtcagctgca agaggctctg gagccagacc
     1081 cgctcctcac agcgctggag tcgcaggact gtgtggagca gcttctgaag aacatgtttg
     1141 atggagaccg gacggagagc tgcctcgtca gtgggactca ggtgttactc accttgctgg
     1201 aaaccaggcg ggttgggaca gagggcttgg tggactcctt ttctcaggga ctggaaaggt
     1261 catacgctgt cagcagcagc gtactacacg gcatcgagcc tcggctgaag gacttccacc
     1321 agctcctgct caacccgccc aagaagaaag cgatcctgac caccattggt gtgctggagg
     1381 agcccctggg gaatgcccgt ctgcatggcg cccgcctcat ggcagcactg ctgcacacaa
     1441 acacacccag catcaaccag gagctctgcc ggctcaacac gatggactta ctgctggact
     1501 tgttctttaa gtacacctgg aataactttt tgcacttcca agtggaacta tgcatagccg
     1561 ctattctctc ccacgctgcc cgtgaggaga ggacagaagc cagcggatcc gagagcaggg
     1621 tggagcctcc gcatgagaac gggaaccgga gcctggagac tccccagccg gccgccagcc
     1681 tccctgacaa cacaatggtg acccacctgt tccagaagtg ctgcctggtg cagaggatcc
     1741 tggaggcctg ggaagccaac gaccacacgc aggcagcggg tggcatgaga cgtgggaaca
     1801 tgggccacct cacacggatc gccaacgcgg tggtgcagaa cctggagcgg ggccctgtgc
     1861 agacgcacat cagcgaggtc atccgagggc tccctgcgga ctgccgtggc cgctgggaga
     1921 gcttcgtgga ggagacgctg acggagacga accgcaggaa cactgtggac ctggtgagca
     1981 ctcaccacct tcactcctca agtgaggacg aggacattga gggtgctttc cctaacgagc
     2041 tgtcccttca gcaggccttc tctgactacc agatccagca gatgacagcc aacttcgtgg
     2101 atcagtttgg cttcaatgat gaggagtttg ccgaccagga cgacaacatc aatgccccgt
     2161 ttgacaggat cgcagagatc aacttcaaca tcgacgctga cgaggacagt cccagcgcag
     2221 ctctgtttga ggcctgctgc agtgaccgca tccagccctt tgatgatgat gaggacgagg
     2281 acatctggga ggacagtgac actcgctgtg ctgcccgggt gatggccaga cccaggtttg
     2341 gagcccccca tgcttcagag agttgctcaa agaatggccc agagcgtgga ggccaggatg
     2401 ggaaggcgag cttggaagca cacagagatg cacctggggc aggtgcccca ccggcccccg
     2461 ggaagaagga agcgccccct gtggagggtg actcagaagg cgccatgtgg acggcagtgt
     2521 ttgatgagcc agcgaactca acgcccacag ccccaggagt ggtgagggac gtgggttcca
     2581 gtgtgtgggc agctggcacc tcagctccag aggagaaagg ctgggccaag ttcactgact
     2641 tccaaccttt ctgctgctcc gagtcagggc ccaggtgcag ctctccggtg gacacagaat
     2701 gcagccatgc tgagggcagc cggagccaag gccctgagaa agccttcagc ccggcttctc
     2761 catgtgcctg gaacgtgtgt gtcaccagga aggcccccct gctggcctct gacagtagct
     2821 cctctggggg ctcccacagc gaggatggcg accagaaggc agcgagtgcc atggatgcgg
     2881 tgagcagggg tcccggccgg gaggcccccc cgctgcccac agtggccagg acagaggagg
     2941 ctgtcggcag ggtcgggtgt gctgacagcc ggctgttaag ccctgcctgc cccgcgccaa
     3001 aggaagtgac tgctgcccca gccgtggctg tgccccccga ggctactgtg gccatcacca
     3061 cagcactgag caaggctggc cccgccatac ccaccccagc agtctcttct gcactggccg
     3121 tggcggtccc cctagggccc atcatggcag tcacagcagc cccagccatg gtggccaccc
     3181 tggggacagt gacaaaggac gggaagacag atgccccgcc agaaggagct gccttaaatg
     3241 gcccagtgtg atgctgctgc cgcccggcca cggcccaccc tggtcaggct gcctccttaa
     3301 tcgagaaaac tacctggtga tgcaatcttt ttttttttta atttaattta attttaaaat
     3361 aaatgctgca ttggtaaagc tggcagttga aaccagttgg acggcccagc ttgcgtctct
     3421 tctgcctgag tgggcctctc aggtcactcg tgccctgctg gaggacagag gggcacctca
     3481 gccgccccca agcccagagc acagcaataa ggtcggcctg caggagccgg ggtgggggtg
     3541 ggggtggggg gggcaggacc ctgagatgcc accaggacct gatgggccag gaagggcgtg
     3601 gacatggagg ctgtttttac agtttttttt ttgttgttgt tttgttttta aagaatacag
     3661 aaggagccaa gcttttttgc actttgtatc cagctgcaag ctcagggcag agtcaagggc
     3721 ctgggttgga aaaacctgac tcacaggaat gcataattga cccttgcagc tacccaatag
     3781 cccttggagc tggcactgaa ccaggctgca agatttgact gccttaaaaa cacaaggccc
     3841 tctaggcctg gcagggatgt ccctgtgccc agcacagggt gcctggcagg gggagaccac
     3901 aggtatgcag gtggggggac atggtgtggc actaggggct cgaagactgg tttctagcac
     3961 taccggtcac ggccatgtcg tcctagaagg gtccagaaga ttattttacg ttgagtccat
     4021 ttttaatgtt ctgatcacct gacagggcac cccaaacccc caactcccaa taaaagccgt
     4081 gacgttcgga caaa
//
