LOCUS       NM_001348144            3961 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 4 regulatory subunit 4 (PPP4R4),
            transcript variant 5, mRNA.
ACCESSION   NM_001348144 XM_005267930
VERSION     NM_001348144.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   3  (bases 1 to 3961)
  AUTHORS   St-Denis,N., Gupta,G.D., Lin,Z.Y., Gonzalez-Badillo,B., Veri,A.O.,
            Knight,J.D.R., Rajendran,D., Couzens,A.L., Currie,K.W., Tkach,J.M.,
            Cheung,S.W.T., Pelletier,L. and Gingras,A.C.
  TITLE     Phenotypic and Interaction Profiling of the Human Phosphatases
            Identifies Diverse Mitotic Regulators
  JOURNAL   Cell Rep 17 (9), 2488-2501 (2016)
   PUBMED   27880917
REFERENCE   4  (bases 1 to 3961)
  AUTHORS   Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
            Goudreault,M. and Gingras,A.C.
  TITLE     PP4R4/KIAA1622 forms a novel stable cytosolic complex with
            phosphoprotein phosphatase 4
  JOURNAL   J Biol Chem 283 (43), 29273-29284 (2008)
   PUBMED   18715871
  REMARK    GeneRIF: PP4R4 forms a novel cytosolic complex with PP4c,
            independent from the complexes containing PP4R1, PP4R2.PP4R3, and
            alpha4, and the regulatory subunits of PP4c have evolved different
            modes of interaction with the catalytic subunit
REFERENCE   5  (bases 1 to 3961)
  AUTHORS   Namciu,S.J., Friedman,R.D., Marsden,M.D., Sarausad,L.M.,
            Jasoni,C.L. and Fournier,R.E.
  TITLE     Sequence organization and matrix attachment regions of the human
            serine protease inhibitor gene cluster at 14q32.1
  JOURNAL   Mamm Genome 15 (3), 162-178 (2004)
   PUBMED   15014966
REFERENCE   6  (bases 1 to 3961)
  AUTHORS   Andrade,M.A., Petosa,C., O'Donoghue,S.I., Muller,C.W. and Bork,P.
  TITLE     Comparison of ARM and HEAT protein repeats
  JOURNAL   J Mol Biol 309 (1), 1-18 (2001)
   PUBMED   11491282
  REMARK    Review article
REFERENCE   7  (bases 1 to 3961)
  AUTHORS   Groves,M.R., Hanlon,N., Turowski,P., Hemmings,B.A. and Barford,D.
  TITLE     The structure of the protein phosphatase 2A PR65/A subunit reveals
            the conformation of its 15 tandemly repeated HEAT motifs
  JOURNAL   Cell 96 (1), 99-110 (1999)
   PUBMED   9989501
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121838.4 and AL117259.6.
            
            On Jun 1, 2019 this sequence version replaced NM_001348144.1.
            
            Summary: The protein encoded by this gene is a HEAT-like
            repeat-containing protein. The HEAT repeat is a tandemly repeated,
            37-47 amino acid long module occurring in a number of cytoplasmic
            proteins. Arrays of HEAT repeats form a rod-like helical structure
            and appear to function as protein-protein interaction surfaces. The
            repeat-containing region of this protein has some similarity to the
            constant regulatory domain of the protein phosphatase 2A PR65/A
            subunit. The encoded protein binds protein serine/threonine
            phosphatase 4c in the cytoplasm. [provided by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (5), as well as variant 4, encodes
            isoform 4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.242664.1,
                                           SRR1803616.256537.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-261               AL121838.4         63569-63829
            262-335             AL121838.4         65301-65374
            336-438             AL121838.4         97711-97813
            439-542             AL117259.6         163194-163297       c
            543-690             AL117259.6         159838-159985       c
            691-764             AL117259.6         159257-159330       c
            765-871             AL117259.6         156813-156919       c
            872-979             AL117259.6         155903-156010       c
            980-1101            AL117259.6         152886-153007       c
            1102-1224           AL117259.6         149777-149899       c
            1225-1394           AL117259.6         148615-148784       c
            1395-1514           AL117259.6         148164-148283       c
            1515-1592           AL117259.6         145860-145937       c
            1593-1676           AL117259.6         144902-144985       c
            1677-1859           AL117259.6         144033-144215       c
            1860-1965           AL117259.6         140295-140400       c
            1966-2113           AL117259.6         138676-138823       c
            2114-2258           AL117259.6         133968-134112       c
            2259-2300           AL117259.6         131748-131789       c
            2301-2375           AL117259.6         131203-131277       c
            2376-2445           AL117259.6         125625-125694       c
            2446-2532           AL117259.6         125099-125185       c
            2533-2626           AL117259.6         124685-124778       c
            2627-2697           AL117259.6         123543-123613       c
            2698-2845           AL117259.6         115051-115198       c
            2846-3961           AL117259.6         110838-111953       c
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12-q32.13"
     gene            1..3961
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="protein phosphatase 4 regulatory subunit 4"
                     /db_xref="GeneID:57718"
                     /db_xref="HGNC:HGNC:23788"
                     /db_xref="MIM:616790"
     exon            1..261
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            262..335
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            336..438
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            439..542
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            543..690
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     CDS             570..2870
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="isoform 4 is encoded by transcript variant 5;
                     HEAT-like repeat-containing protein;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     4; cilia and flagella associated protein 14"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 4 isoform 4"
                     /protein_id="NP_001335073.1"
                     /db_xref="GeneID:57718"
                     /db_xref="HGNC:HGNC:23788"
                     /db_xref="MIM:616790"
                     /translation="MQLTAAMSFLTILQDESVSIHAYTHSFLQVILLHLEHRDTGVSN
                     AWLETLLSVIEVLPKETLRHEILNPLVSKAQLSQTVQSRLVSCKILGKLTNKFDAHTI
                     KREILPLVKSLCQDVEYEVRSCMCRQLENIAQGIGTELTKSVVLPELIELSRDEGSSV
                     RLAAFETLVNLLDIFDTDDRSQTILPLVKSFCEKSFKADESILISLSFHLGKLCHGLY
                     GIFTPDQHLRFLEFYKKLCTLGLQQENGHNENQIPPQILEQEKKYISVRKNCAYNFPA
                     MIVFVDPKNFHMELYSTFFCLCHDPEVPVRYTIAICFYEVSKLLNSGVYLIHKELITL
                     LQDESLEVLDALIDHLPEILELMSTGGESSVQENKLSSLPDLIPALTAAEQRAAASLK
                     WRTHEKLLQKYACLPHVISSDQIYYRFLQRMFTIMMTNNVLPVQKAASRTLCIFLRYN
                     RKQEQRHEVIQKLIEQLGQGKSYWNRLRFLDTCEFIIEIFSKSFFCKYFFLPAIELTH
                     DPVANVRMKLCYLLPKVKSTLKIPADKHLLQQLEMCVRKLLCQEKDKDVLAIVKRTVL
                     ELDRMEMSMDAFQKKFYEKDLLDQEKEREELLLLEMEQLEKEKQQNDGRPMSDKMFEK
                     KRRDTKTPTQSLPKNIPISVPGPSSVTPSTSKEIKKSKLIRSQSFNNQAFHAKYGNLE
                     KCASKSSTTGYTTSVSGLGKTSVLSLADDSFRTRNASSVPSSFSPNTPLPSTSRGTGN
                     SVDPKSSGSKDTQPRKATLKSRKSNP"
     exon            691..764
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            765..871
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            872..979
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1101
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1224
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1394
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1514
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1592
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1676
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1859
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1965
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2113
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2258
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2300
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2375
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2445
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2532
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2626
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..2697
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2845
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..3961
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3940..3945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="hexamer: AATAAA"
     polyA_site      3961
                     /gene="PPP4R4"
                     /gene_synonym="CFAP14; KIAA1622; PP4R4"
                     /note="major polyA site"
ORIGIN      
        1 cttgctcccg ccgcctggca gcctcacgct cggctccagc ggccaagagc cggagaaagt
       61 cctgctggtg ggcggccgcg gggctgaggg cgtccggcat cccggggccg ctccggcccg
      121 ggcggcgaga gtgcccggcg gtccatgcat ccgccgccgc ccgccgccgc gatggatttc
      181 agtcagaaca gcctgttcgg ttacatggag gacctgcagg agctcaccat catcgagagg
      241 ccggtccgcc ggagcctcaa gacaccggaa gaaatagaaa gattgacagt cgatgaagac
      301 ctcagtgata ttgaaagggc tgtttatctg ctcagtgctg gtcaagatgt ccaaggaaca
      361 agtgtgattg caaatctccc atttttgatg cgacagaatc ccactgagac gcttcggaga
      421 gtgttgccaa aagtcagatg tcttccaagg tccatgagga tgcacactta tttatccaga
      481 gagtatggat ctcacatatg tttgtccaga gggtgtgact ctgtggagga gaacacttac
      541 tggaagccct gcatgttgca ggagtggaaa tgcagttaac ggctgcgatg tcatttctga
      601 ccattctgca ggacgaatca gtgtcaattc atgcatatac ccactcattc ctccaagtca
      661 ttctcctgca tctggagcac agggacacag gtgtcagcaa tgcatggctg gaaactcttc
      721 tgtctgttat agaagtattg ccaaaagaaa ccctacggca tgagattttg aatccacttg
      781 tttccaaggc acaactttcc caaacagtcc agtctcgttt agttagttgt aaaattttag
      841 gaaaattgac caacaaattt gatgcccaca ccattaagcg agaaatactt cctctggtaa
      901 aatcactctg tcaagatgta gaatatgaag ttcgatcttg tatgtgtcgg caattagaaa
      961 atatagccca gggcattggg acagaactta caaaaagtgt ggtgctccct gaattaatag
     1021 aactttctag ggatgaaggc agcagtgtac gacttgcagc ttttgaaact ttggttaatc
     1081 tgcttgatat atttgataca gatgacagaa gtcaaactat acttccctta gtgaaatcat
     1141 tttgtgaaaa atctttcaaa gcagatgaat caattcttat ttctttatct ttccatttag
     1201 gaaaactatg tcatggacta tatggaattt tcactccaga tcagcacttg agatttttgg
     1261 aattttataa gaaactttgt acattgggtt tgcaacaaga aaatggacac aatgaaaacc
     1321 agattccacc ccaaatccta gagcaggaga agaaatatat ttcagtacgg aagaactgtg
     1381 cttataactt tccggccatg attgtttttg ttgatcctaa aaacttccac atggaactct
     1441 attctacatt cttctgcctt tgccatgacc ctgaagtacc agtcagatac actattgcta
     1501 tttgctttta tgaagtatct aagcttctga attctggagt atatttaata cataaagaac
     1561 taataacatt attacaagat gaatcactgg aggtactaga tgctcttata gatcatcttc
     1621 cagaaatctt ggaacttatg tctactggtg gagaaagcag tgttcaagaa aataagttat
     1681 cttctctgcc tgacttgatt ccagcactca cagctgctga acagcgagct gcagcctctt
     1741 taaaatggag aactcatgag aagctacttc agaaatatgc ctgcctgcca catgtcatat
     1801 caagcgatca gatttattac cgtttcttac aaagaatgtt cacaatcatg atgacaaata
     1861 atgttttacc tgtccaaaag gcggcttcac gaactctatg catttttctg cgttataatc
     1921 gtaaacaaga acagagacat gaggtcattc aaaaattaat tgaacaattg ggccaaggaa
     1981 aaagttactg gaatagactt cgatttttgg atacctgtga atttattata gagatatttt
     2041 caaaatcatt tttctgtaaa tatttctttc tacctgctat tgaactgaca catgatccag
     2101 tagcaaatgt gagaatgaaa ctttgctacc tgttgcccaa agtgaaatct actctgaaga
     2161 ttcctgctga taagcatcta cttcagcagt tagaaatgtg tgtgaggaaa ctcctgtgtc
     2221 aagaaaaaga taaagatgtt ctggctattg taaaaagaac tgtattagag ttggacagaa
     2281 tggaaatgtc tatggatgct tttcagaaaa agttttatga gaaagatttg ttggatcaag
     2341 agaaagaaag agaagaacta cttcttttgg aaatggaaca attagagaaa gaaaagcaac
     2401 agaatgatgg aaggcccatg agtgataaaa tgtttgaaaa gaaacgtaga gacactaaga
     2461 caccaacgca aagtctgccc aagaacatcc ccatttctgt tcctggaccc tcttctgtca
     2521 ccccatcgac aagtaaagaa atcaagaaat ccaaactgat tcgaagccag tcttttaata
     2581 atcaagcttt tcatgcaaaa tatggcaact tagagaaatg tgctagtaaa agttctacaa
     2641 caggatatac aacttctgtc tcagggttag gaaagacttc tgtgctttca ctagctgatg
     2701 attcattccg gactcgtaat gccagtagcg ttccatcttc cttttctcct aatactccct
     2761 taccgagtac ttcccgtggg acaggtaact cagttgaccc caagagcagt ggaagtaaag
     2821 atacacaacc acggaaggct accttaaaat ccagaaaatc caatccttaa atcaactgct
     2881 tgatgaagga ggcaaaacaa aggcagcagg agataatgtg attggtacac aaaagctaaa
     2941 gcagtggctg gggctttgtt tttaaatttt gggttttttt ttttgttgtt gttaatgagc
     3001 agaaagagag acataatgac agctgatgtt aaacttttca tatttcaaat tagattccct
     3061 aggaggtata atatatattt cttgagtaat aatgtggtta cggaattcca atgttatagt
     3121 gaagtgtaat gaaaaacatc tctaggaatg tgctttaacc actgctgcaa aagagacaag
     3181 tctgcattta tttgtgcagg aaaccagcca tttaattgtt ctagagtttt agcatttaaa
     3241 aatcgtatga aagtctacat cagctgaatt gtcctagctt gataagcact tggaggggga
     3301 cttggaaggt gagaaagata ctgcattttc tcatgagtct ccaagcccac ttgaaaagtc
     3361 acactgaaag gatgcaaata gccgtctgcg attttggcgg ccctccggat tgtggtgaca
     3421 atatgttgta cccggacatt cccaaattta aatttggcag tgaaaattcc acaaagattt
     3481 ctggtaactt tgagctataa ataccttaaa aataatagat tgatgatttt ctttatttcc
     3541 tagagaattt attttataaa tactttgttt acattttaga ttgtacttgt ctttatttaa
     3601 aatgatgaat ctagtctgaa tcagctgtta ttccaagcta tcatgcttaa gctatgtcaa
     3661 cagcatttat ttgtactaat gctaatattt ccatcaaaat ttgcctgtgg aatatataca
     3721 gttcttaatt ttaaactgtc agttcttgag atataccgtg tgaagctatt gtcagcttct
     3781 ctatttcaaa atgcaatcag catataacta tattgatatt agaagatatt tgttttttaa
     3841 aatatattga tgtatgataa agatgatcta atttgtgaat gcatatgtat gtgtggttac
     3901 tttttataat gtgaaataat gaataatgaa tttactcaaa ataaaacata ggttaatgag
     3961 a
//
