LOCUS       NM_001122964            5506 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens protein phosphatase 4 regulatory subunit 3B (PPP4R3B),
            transcript variant 1, mRNA.
ACCESSION   NM_001122964
VERSION     NM_001122964.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Kristiansson,K., Perola,M., Tikkanen,E., Kettunen,J., Surakka,I.,
            Havulinna,A.S., Stancakova,A., Barnes,C., Widen,E., Kajantie,E.,
            Eriksson,J.G., Viikari,J., Kahonen,M., Lehtimaki,T.,
            Raitakari,O.T., Hartikainen,A.L., Ruokonen,A., Pouta,A., Jula,A.,
            Kangas,A.J., Soininen,P., Ala-Korpela,M., Mannisto,S.,
            Jousilahti,P., Bonnycastle,L.L., Jarvelin,M.R., Kuusisto,J.,
            Collins,F.S., Laakso,M., Hurles,M.E., Palotie,A., Peltonen,L.,
            Ripatti,S. and Salomaa,V.
  TITLE     Genome-wide screen for metabolic syndrome susceptibility Loci
            reveals strong lipid gene contribution but no evidence for common
            genetic basis for clustering of metabolic syndrome traits
  JOURNAL   Circ Cardiovasc Genet 5 (2), 242-249 (2012)
   PUBMED   22399527
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
            Goudreault,M. and Gingras,A.C.
  TITLE     PP4R4/KIAA1622 forms a novel stable cytosolic complex with
            phosphoprotein phosphatase 4
  JOURNAL   J Biol Chem 283 (43), 29273-29284 (2008)
   PUBMED   18715871
REFERENCE   3  (bases 1 to 5506)
  AUTHORS   Chowdhury,D., Xu,X., Zhong,X., Ahmed,F., Zhong,J., Liao,J.,
            Dykxhoorn,D.M., Weinstock,D.M., Pfeifer,G.P. and Lieberman,J.
  TITLE     A PP4-phosphatase complex dephosphorylates gamma-H2AX generated
            during DNA replication
  JOURNAL   Mol Cell 31 (1), 33-46 (2008)
   PUBMED   18614045
  REMARK    GeneRIF: A three-protein PP4 phosphatase complex in mammalian
            cells, containing PP4C, PP4R2, and PP4R3beta, specifically
            dephosphorylates ATR-mediated gamma-H2AX generated during DNA
            replication.
REFERENCE   4  (bases 1 to 5506)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   5  (bases 1 to 5506)
  AUTHORS   Gingras,A.C., Caballero,M., Zarske,M., Sanchez,A., Hazbun,T.R.,
            Fields,S., Sonenberg,N., Hafen,E., Raught,B. and Aebersold,R.
  TITLE     A novel, evolutionarily conserved protein phosphatase complex
            involved in cisplatin sensitivity
  JOURNAL   Mol Cell Proteomics 4 (11), 1725-1740 (2005)
   PUBMED   16085932
REFERENCE   6  (bases 1 to 5506)
  AUTHORS   Mendoza,M.C., Du,F., Iranfar,N., Tang,N., Ma,H., Loomis,W.F. and
            Firtel,R.A.
  TITLE     Loss of SMEK, a novel, conserved protein, suppresses MEK1 null cell
            polarity, chemotaxis, and gene expression defects
  JOURNAL   Mol Cell Biol 25 (17), 7839-7853 (2005)
   PUBMED   16107728
REFERENCE   7  (bases 1 to 5506)
  AUTHORS   Kittler,R., Putz,G., Pelletier,L., Poser,I., Heninger,A.K.,
            Drechsel,D., Fischer,S., Konstantinova,I., Habermann,B.,
            Grabner,H., Yaspo,M.L., Himmelbauer,H., Korn,B., Neugebauer,K.,
            Pisabarro,M.T. and Buchholz,F.
  TITLE     An endoribonuclease-prepared siRNA screen in human cells identifies
            genes essential for cell division
  JOURNAL   Nature 432 (7020), 1036-1040 (2004)
   PUBMED   15616564
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            KF510821.1 and AC015982.9.
            
            On Nov 29, 2018 this sequence version replaced NM_001122964.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.243610.1,
                                           SRR1803613.78145.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000616407.2/ ENSP00000483228.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               KF510821.1         39-363
            326-479             AC015982.9         97042-97195         c
            480-535             AC015982.9         95349-95404         c
            536-634             AC015982.9         83876-83974         c
            635-1258            AC015982.9         78314-78937         c
            1259-1336           AC015982.9         68777-68854         c
            1337-1453           AC015982.9         66516-66632         c
            1454-1570           AC015982.9         64949-65065         c
            1571-1702           AC015982.9         61465-61596         c
            1703-1805           AC015982.9         59577-59679         c
            1806-1901           AC015982.9         58145-58240         c
            1902-1943           AC015982.9         57213-57254         c
            1944-2102           AC015982.9         53517-53675         c
            2103-2272           AC015982.9         48092-48261         c
            2273-2412           AC015982.9         44800-44939         c
            2413-2597           AC015982.9         44211-44395         c
            2598-2791           AC015982.9         38673-38866         c
            2792-5506           AC015982.9         27190-29904         c
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.1"
     gene            1..5506
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /note="protein phosphatase 4 regulatory subunit 3B"
                     /db_xref="GeneID:57223"
                     /db_xref="HGNC:HGNC:29267"
                     /db_xref="MIM:610352"
     exon            1..479
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             338..2887
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     3B; SMEK homolog 2, suppressor of mek1"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 3B isoform 1"
                     /protein_id="NP_001116436.3"
                     /db_xref="CCDS:CCDS46289.1"
                     /db_xref="GeneID:57223"
                     /db_xref="HGNC:HGNC:29267"
                     /db_xref="MIM:610352"
                     /translation="MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRA
                     ESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQG
                     KDPSVEVTQDLIDESEEERFEEMPETSHLIDLPTCELNKLEEIADLVTSVLSSPIRRE
                     KLALALENEGYIKKLLQLFQACENLENTEGLHHLYEIIRGILFLNKATLFEVMFSDEC
                     IMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDII
                     LPTPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELV
                     NFFKEFCAFSQTLQPQNRDAFFKTLAKLGILPALEIVMGMDDLQVRSAATDIFSYLVE
                     FSPSMVREFVMQEAQQSDDDILLINVVIEQMICDTDPELGGAVQLMGLLRTLIDPENM
                     LATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKDFFLKHYRYSWSFICTPSH
                     SHSHSTPSSSISQDNIVGSNKNNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYI
                     MNKDLLRRVLVLMNSKHTFLALCALRFMRRIIGLKDEFYNRYITKGNLFEPVINALLD
                     NGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKALESIEYVQTFKGLKTKYEQE
                     KDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVVAPVEKPKPEDD
                     FPDNYEKFMETKKAKESEDKENLPKRTSPGGFKFTFSHSASAANGTNSKSVVAQIPPA
                     TSNGSSSKTTNLPTSVTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS"
     exon            480..535
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..634
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            635..1258
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1336
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1453
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1570
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1702
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1805
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1901
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..1943
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2102
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2272
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2412
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2597
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2791
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..5506
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5484..5489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /note="hexamer: AATAAA"
     polyA_site      5506
                     /gene="PPP4R3B"
                     /gene_synonym="FLFL2; PP4R3B; PSY2; SMEK2; smk1"
                     /note="major polyA site"
ORIGIN      
        1 gaagagaaag gcagagagag tcgggttaca agatggcgga tctgtagtag ttaccgcggc
       61 ggcgggagag caagcgagcc ctggggggca aagagacggg agagtgggtg tatgcgcggg
      121 tgaagtgaga ggtaacgggg cctccgggcg gagaggcctc agtggctctt gtcacccctt
      181 ctcgcggctg aacctttgga gccatggtga attcgggcct ctccgaagcc gccgccgccg
      241 ccaccgccac tactgccttt accgtctcct aagagtgagg agcgcggacg aggtaagcga
      301 ggaggcggcg gctagagcgg tggagacagc agccaccatg tcggatacgc ggcggcgagt
      361 gaaggtctat accctgaacg aagaccggca atgggacgac cgaggcaccg ggcacgtctc
      421 ctccacttac gtggaggagc tcaaggggat gtcgctgctg gttcgggcag agtccgacgg
      481 atcactactc ttggaatcaa agataaatcc aaatactgca tatcagaaac aacaggatac
      541 attaattgtt tggtcagaag cagagaacta tgatttggct ctgagttttc aggagaaagc
      601 tggctgtgat gagatctggg aaaaaatttg tcaggttcaa ggtaaagacc catcagtgga
      661 agtcacacag gacctcattg atgaatctga agaagaacga tttgaagaaa tgcctgaaac
      721 tagtcatctg attgacctgc ccacatgtga actcaataaa cttgaagaga ttgctgactt
      781 agttacctca gtgctctcct cacctatccg tagggaaaag ctggctctcg ccttggaaaa
      841 tgaaggctat attaaaaaac tattgcagct gttccaagct tgcgagaacc tagaaaacac
      901 tgaaggctta caccatttgt atgaaattat tagaggaatc ttattcctaa ataaggcaac
      961 tctttttgag gtaatgtttt ctgatgagtg tatcatggat gtcgtgggat gccttgaata
     1021 tgaccctgct ttggctcagc caaaaagaca tagagaattc ttgaccaaaa ctgcaaagtt
     1081 caaggaagtt ataccaataa cagactctga actaaggcaa aaaatacatc agacttacag
     1141 ggtacagtac attcaggaca tcattttgcc cacaccatct gtttttgaag agaattttct
     1201 ttctactctt acgtctttta ttttcttcaa caaagttgag atagtcagca tgttgcagga
     1261 agatgagaag tttttgtctg aagtttttgc acaattaaca gatgaggcta cagatgatga
     1321 taaacggcgt gaattggtta attttttcaa ggagttttgt gcattttctc agacattaca
     1381 acctcaaaac agggatgcat ttttcaaaac attggcaaaa ttgggaattc ttcctgctct
     1441 tgaaattgta atgggcatgg atgatttgca agtcagatca gctgctacag atatattttc
     1501 ttatctagta gaatttagtc catctatggt ccgagagttt gtaatgcaag aagctcagca
     1561 gagtgatgac gatattcttc ttattaatgt ggtaattgaa caaatgatct gtgatactga
     1621 tcctgagcta ggaggcgctg ttcagttaat gggacttctt cgtactctaa ttgatccaga
     1681 gaacatgctg gctacaacta ataaaaccga aaaaagtgaa tttctaaatt ttttctacaa
     1741 ccattgtatg catgttctca cagcaccact tttgaccaat acttcagaag acaaatgtga
     1801 aaaggatttt tttttaaaac attacagata tagttggagt ttcatatgta ccccttcaca
     1861 ttcccattcc cattctaccc cctcttcctc catctctcaa gataatatag ttggatcaaa
     1921 caaaaacaac acaatttgtc ccgataatta tcaaacagca cagctacttg ccttaatttt
     1981 agagttactc acattttgtg tggaacatca cacatatcac ataaaaaact atattatgaa
     2041 caaggacttg ctaagaagag tcttggtctt gatgaattca aagcacactt ttctggcctt
     2101 gtgtgccctt cgctttatga ggcggataat tggacttaaa gatgaatttt ataatcgtta
     2161 catcaccaag ggaaatcttt ttgagccagt tataaatgca cttctggata atggaactcg
     2221 gtataatctg ttgaattcag ctgttattga gttgtttgaa tttataagag tggaagatat
     2281 caagtctctt actgcccata tagttgaaaa cttttataaa gcacttgaat cgattgaata
     2341 tgttcagaca ttcaaaggat tgaagactaa atatgagcaa gaaaaagaca gacaaaatca
     2401 gaaactgaac agtgtaccat ctatattgcg tagtaacaga tttcgcagag atgcaaaagc
     2461 cttggaagag gatgaagaaa tgtggtttaa tgaagatgaa gaagaggaag gaaaagcagt
     2521 tgtggcacca gtggaaaaac ctaagccaga agatgatttt ccagataatt atgaaaagtt
     2581 tatggagact aaaaaagcaa aagaaagtga agacaaggaa aaccttccca aaaggacatc
     2641 tcctggtggc ttcaaattta ctttctccca ctctgccagt gctgctaatg gaacaaacag
     2701 taaatctgta gtggctcaga taccaccagc aacttctaat ggatcctctt ccaaaaccac
     2761 aaacttgcct acgtcagtaa cagccaccaa gggaagtttg gttggcttag tggattatcc
     2821 agatgatgaa gaggaagatg aagaagaaga atcgtccccc aggaaaagac ctcgtcttgg
     2881 ctcataaaat atttattagg ggaccctcaa catgtggtct tacaatgctg caactgttca
     2941 gtgagctgaa aatctgaatc agaaagcttt ctcaattgaa cttataaaat atacaaggag
     3001 tagcaaaaga cagtatatca gctaagagag tttagttcta ataaaaatca ggcttcccag
     3061 gaacttgatt gcttgctagt aattaagggg tttgcctttt aggctgtcaa aacaaacatt
     3121 agtaaccaga acctgggaga cagcttctca gcaaggaaaa gtcacaggtt tggggacggt
     3181 ttaggggagg ggaaaaggtt gatataataa tgcagggttg ctcctcgggg tgtcgatcta
     3241 gaaacaattt tacagaactt cagttgtaaa ctcaataaca ttacttgtat aatggtgctg
     3301 gccatgttgt tgttttaatc agttgcctct ttttaaaaga aatttttatg gaaaacacat
     3361 tcaactatca ttaaaaaaat gaagttaagc tgttgggacc atttctttaa gatttaacaa
     3421 aagttcagcc ttttaggtag ttgaagggaa gtacaccccg tattcagcac atgttgagtt
     3481 ttctacacca ggaattttca atatgtatat tgatgaaaac aagctcaatt caaactggac
     3541 agttttaaga taatgttaaa atcagcactt ttagagacaa cgaaggccaa gaatcagtac
     3601 agtagtattc caaaatgatt ttctctagaa atttgaaagt agatcgaaca gaatgttgtc
     3661 aaccgcctac cagtacaatc ttttgtggaa gatactttga aatcactttc tactttgtta
     3721 gtaaagttct gtctttccag agctgcaagt tttaaagtgt tacttataca gaccaaccaa
     3781 gaatagtgct gaattaagtg gcatttagta tctagaagcc attttgatcc aagaagctac
     3841 ttaagtgtca aagtcagcat gcagcacatg tagcttttct gtaaacaagg gtgtgatatg
     3901 aaagctgctt ttttaagaag agtaaaagca cattccatat acgtaagtga attttaaaaa
     3961 taaattgagg caaacagtta agttttattt ttagagcaac aagttaactg taaatatttt
     4021 aatgttagtt tgctcatcta tgatctgaga tcatgccgaa gtgagaaaaa tctccccaaa
     4081 atacaattta atgcattggg aaaaaaaaac tttaacagta attccagcca caatctttag
     4141 atcacccttg taatgtgtta cgggtccatt tttcctggaa tcgtttaatc taaagcagtt
     4201 tcccctgttt tggagatttt gtagttaatt ttaattttgg ctattgtttg gaaaagatga
     4261 gctgtctgtg tagatatgaa gtatagtttt ttccataaaa cagatgttta ttttgtatta
     4321 aaaaatacca ctgtacttgt tttacaccat ttgtatacat gtggtgatat taatgctaaa
     4381 ctgtaaaatt caggaattaa aatgtgaccc tgtaattcca taattgatgt ttgtgtttgg
     4441 tttgttgtaa atataaatta actccatttt gagatatgtc aattttacag tatcttttaa
     4501 aagccaataa aattatccat aaatatttat tataaactgc tagatcatac cccaatttta
     4561 atgaaataat tctaaacaaa tgcgggtcaa tggcacttta tcagtgacta cttatagaag
     4621 gtaagccttg gggaaatcat agcatcacag tatggagctt ttctcacctt ttcaattaaa
     4681 ggtgtacata tagtaagaaa gaaagacctt ccacctcata aggcaggtga ctcaggatat
     4741 tttaaactaa gatcagtaaa gttcccatcc actttctttg aatcatcaaa ttttatcgag
     4801 acccatctct cagcttttag taaatttctt cttaaaataa gcccacatgt aaaacacttt
     4861 cctagttact ttttgtttgt ttgtttgagt ctcgctctgt tgcccaggct ggagtgtagt
     4921 tgcacgattt cggcttattg caacatccgc cacctgggtt caagcaattc tctgcctcag
     4981 cctgagtagc tgggattaca ggtgcccgtc accatgccag gctaattttt gtattttcag
     5041 tagagacggg gtttcaccat cttggccagg ctggtcttaa actcctgacc tcacaatcca
     5101 cccgcctcag cctccctatc tctgtatctt atgaagactt cctttttctt tctgaagagc
     5161 tgccataagt cttaaacatt taaatcatca gaaccttttc ttgtagcaga aatgtgaatg
     5221 ttttcttgaa gtacaggtta cggttttcat ggtacaagtg cttacctacg acgataagag
     5281 aacaaggctt aaaatatccc tcaaaagaac aattgtgaga attccatgtt agcggctgtc
     5341 tttaaacgac taccctcagg cagatctggg tttttatatc tttatcttca ttcagtgaag
     5401 caagccaaaa gcccacattt gtatgcctta ggtcttctta aaatggtatc tgtaaacatg
     5461 tgtccaatat aaaaactatt tttaataaaa agttacatga aacaga
//
