LOCUS       NM_174907               4957 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 4 regulatory subunit 2 (PPP4R2),
            transcript variant 1, mRNA.
ACCESSION   NM_174907 NM_019853
VERSION     NM_174907.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   3  (bases 1 to 4957)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   4  (bases 1 to 4957)
  AUTHORS   St-Denis,N., Gupta,G.D., Lin,Z.Y., Gonzalez-Badillo,B., Veri,A.O.,
            Knight,J.D.R., Rajendran,D., Couzens,A.L., Currie,K.W., Tkach,J.M.,
            Cheung,S.W.T., Pelletier,L. and Gingras,A.C.
  TITLE     Phenotypic and Interaction Profiling of the Human Phosphatases
            Identifies Diverse Mitotic Regulators
  JOURNAL   Cell Rep 17 (9), 2488-2501 (2016)
   PUBMED   27880917
REFERENCE   5  (bases 1 to 4957)
  AUTHORS   Hasegawa,Y., Taylor,D., Ovchinnikov,D.A., Wolvetang,E.J., de
            Torrente,L. and Mar,J.C.
  TITLE     Variability of Gene Expression Identifies Transcriptional
            Regulators of Early Human Embryonic Development
  JOURNAL   PLoS Genet 11 (8), e1005428 (2015)
   PUBMED   26288249
  REMARK    GeneRIF: Results from a study on gene expression variability
            markers in early-stage human embryos shows that PPP4R2 is a
            putative expression variability marker for the 3-day, 8-cell embryo
            stage.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4957)
  AUTHORS   Chen,G.I., Tisayakorn,S., Jorgensen,C., D'Ambrosio,L.M.,
            Goudreault,M. and Gingras,A.C.
  TITLE     PP4R4/KIAA1622 forms a novel stable cytosolic complex with
            phosphoprotein phosphatase 4
  JOURNAL   J Biol Chem 283 (43), 29273-29284 (2008)
   PUBMED   18715871
REFERENCE   7  (bases 1 to 4957)
  AUTHORS   Chowdhury,D., Xu,X., Zhong,X., Ahmed,F., Zhong,J., Liao,J.,
            Dykxhoorn,D.M., Weinstock,D.M., Pfeifer,G.P. and Lieberman,J.
  TITLE     A PP4-phosphatase complex dephosphorylates gamma-H2AX generated
            during DNA replication
  JOURNAL   Mol Cell 31 (1), 33-46 (2008)
   PUBMED   18614045
  REMARK    GeneRIF: A three-protein PP4 phosphatase complex in mammalian
            cells, containing PP4C, PP4R2, and PP4R3beta, specifically
            dephosphorylates ATR-mediated gamma-H2AX generated during DNA
            replication.
REFERENCE   8  (bases 1 to 4957)
  AUTHORS   Gingras,A.C., Caballero,M., Zarske,M., Sanchez,A., Hazbun,T.R.,
            Fields,S., Sonenberg,N., Hafen,E., Raught,B. and Aebersold,R.
  TITLE     A novel, evolutionarily conserved protein phosphatase complex
            involved in cisplatin sensitivity
  JOURNAL   Mol Cell Proteomics 4 (11), 1725-1740 (2005)
   PUBMED   16085932
REFERENCE   9  (bases 1 to 4957)
  AUTHORS   Carnegie,G.K., Sleeman,J.E., Morrice,N., Hastie,C.J., Peggie,M.W.,
            Philp,A., Lamond,A.I. and Cohen,P.T.
  TITLE     Protein phosphatase 4 interacts with the Survival of Motor Neurons
            complex and enhances the temporal localisation of snRNPs
  JOURNAL   J Cell Sci 116 (Pt 10), 1905-1913 (2003)
   PUBMED   12668731
REFERENCE   10 (bases 1 to 4957)
  AUTHORS   Hastie,C.J., Carnegie,G.K., Morrice,N. and Cohen,P.T.
  TITLE     A novel 50 kDa protein forms complexes with protein phosphatase 4
            and is located at centrosomal microtubule organizing centres
  JOURNAL   Biochem J 347 Pt 3 (Pt 3), 845-855 (2000)
   PUBMED   10769191
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC098481.2 and AC103559.2.
            
            On May 17, 2019 this sequence version replaced NM_174907.3.
            
            Summary: The protein encoded by this gene is a regulatory subunit
            of the serine/threonine-protein phosphatase 4 complex. In addition
            to being required for efficient DNA double strand break repair,
            this complex plays a role in organization of microtubules at
            centrosomes and processing of spliceosomal snRNPs. Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.253802.1,
                                           SRR1803613.53653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356692.10/ ENSP00000349124.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-261               AC098481.2         175248-175508
            262-343             AC098481.2         176514-176595
            344-514             AC098481.2         225623-225793
            515-608             AC103559.2         113719-113812
            609-646             AC103559.2         115705-115742
            647-721             AC103559.2         118355-118429
            722-865             AC103559.2         118685-118828
            866-1155            AC103559.2         119534-119823
            1156-4957           AC103559.2         120079-123880
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p13"
     gene            1..4957
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /note="protein phosphatase 4 regulatory subunit 2"
                     /db_xref="GeneID:151987"
                     /db_xref="HGNC:HGNC:18296"
                     /db_xref="MIM:613822"
     exon            1..261
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..1481
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein phosphatase 4 regulatory subunit
                     2"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 2 isoform 1"
                     /protein_id="NP_777567.1"
                     /db_xref="CCDS:CCDS2917.1"
                     /db_xref="GeneID:151987"
                     /db_xref="HGNC:HGNC:18296"
                     /db_xref="MIM:613822"
                     /translation="MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQ
                     FKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQ
                     RLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSP
                     SYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTS
                     ESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGET
                     EASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQEKESDDALT
                     VNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSDCRETEELVGSNSSKTG
                     EILSESSMENDDEATEVTDEPMEQD"
     exon            262..343
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            344..514
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            515..608
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            609..646
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            647..721
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            722..865
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1155
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..4957
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2931..2936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /note="hexamer: AATAAA"
     polyA_site      2952
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /note="major polyA site"
     regulatory      4937..4942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
                     /note="hexamer: AATAAA"
     polyA_site      4957
                     /gene="PPP4R2"
                     /gene_synonym="PP4R2"
ORIGIN      
        1 gggcgccatg ttggagggtt ggtggtagcg gcttggggag gtgctcgctc tgtcggtctt
       61 gctctctcgc acgcttcccc cggctccctt cgtttccccc ccccggtcgc ctgcgtgccg
      121 gagtgtgtgc gagggagggg gagggcgtcg ggggggtggg gggaggcgtt ccggtcccca
      181 agagacccgc ggagggaggc ggaggctgtg agggactccg ggaagccatg gacgtcgaga
      241 ggctccagga ggcgctgaaa gattttgaga agagggggaa aaaggaagtt tgtcctgtcc
      301 tggatcagtt tctttgtcat gtagccaaga ctggagaaac aatgattcag tggtcccaat
      361 ttaaaggcta ttttattttc aaactggaga aagtgatgga tgatttcaga acttcagctc
      421 ctgagccaag aggtcctccc aaccctaatg tcgaatatat tccctttgat gaaatgaagg
      481 aaagaatact gaaaattgtc actggattta atggtatccc ttttactatt cagcgactat
      541 gtgaattgtt aacagatcca aggagaaact atacaggaac agacaaattt ctcagaggag
      601 tagaaaagaa tgtgatggtt gttagctgtg tttatccttc ttcagagaaa aacaattcca
      661 atagtttaaa tcgaatgaat ggtgttatgt ttcctggaaa ttcaccaagc tatactgaga
      721 ggtctaatat aaatgggcct gggacaccca ggccacttaa tcgaccaaag gtttctttgt
      781 cagcccccat gacaacaaat gggttgcctg agagcacaga cagcaaagag gcaaatttgc
      841 agcaaaatga ggagaaaaat cacagtgact cttcgacctc tgaatcagaa gtttcctcag
      901 tgagcccttt gaaaaataaa catccagatg aagatgctgt ggaagctgag gggcatgagg
      961 taaaaagact caggtttgac aaagaaggtg aagtcagaga aacagccagt caaacgactt
     1021 ccagcgaaat ttcttcagtt atggtaggag aaacagaagc atcatcttca tctcaggata
     1081 aagacaaaga tagccgttgt acccggcagc actgtacaga agaggatgaa gaagaggatg
     1141 aagaggaaga agaagagtct tttatgacat caagagaaat gatcccagaa agaaaaaatc
     1201 aagaaaaaga atctgatgat gccttaactg tgaatgaaga gacttctgag gaaaataatc
     1261 aaatggagga atctgatgtg tctcaagctg agaaagattt gctacattct gaaggtagtg
     1321 aaaacgaagg ccctgtaagt agtagttctt ctgactgccg tgaaacagaa gaattagtag
     1381 gatccaattc cagtaaaact ggagagattc tttcagaatc atccatggaa aatgatgacg
     1441 aagccacaga agtcaccgat gaaccaatgg aacaagacta actatttaga aacatttaga
     1501 tgcagtattt tacatacagt tctggtttta acactgtata aaacttttgt gtaataaaat
     1561 ggacctttag ttttacaaga gaagcaggtt gtaaaataaa gtactttatg gataattcct
     1621 gaaagagttg tacatgtaag aactgtgaat atcagctcct ctgggtcctg cttaccttac
     1681 cgctgacttt tctttctttc tttttttggt ctgggcaaat cagtggtttg tgtatagatt
     1741 tttttttttt tttaatttag gattgaagtt tttaaactgg aaagtaatta caattttgaa
     1801 aagttttttg agattatcac atttagttta tacatatgca agaagctttt tgtcttgtct
     1861 ctttctgata gctctagcag ttttcatatt ttggtcatag tttcaacatt ttaacatgtg
     1921 aattataggg tttcatgctg gtttccagat tttattgttt ggctacgtac aatggaactt
     1981 taagtcatat atacatacat atatatatat atatatatat aattctaagg ggggaaatgt
     2041 tatatttttc tgtttctata agagatgaat acagtggata ctttttctat tggtaatgat
     2101 tgagttcacc tctttcagaa gacattttct ttctcttctg agtaattgaa ataaaatctg
     2161 gcccttgtga aaccctggaa atcttaagtc tgttgaaata ccaggttaaa cacattccaa
     2221 gagatctgtt caaactcaaa ttcttttgta tacttctgag gtgcctgaga aaaagacttc
     2281 attatttatg agaaaatatg ctttatcttg gaaattgtgt tcaaatgtta gcttactatt
     2341 ttgtagaatg aatgtttatg aagctgatat gagaccatct cagaagaacc aagtaggttc
     2401 cttgaccttt tgcttgcttt tctgaacatt gtgaatatta cacatgtctt tctaaattat
     2461 tctagggtat gcaaatgtca atggtatgaa acaccactgt actggaagaa ttaatatatt
     2521 actttagtat gtacctgagc taaatgactg aagctttagg ggtgcataga aaccaccata
     2581 atttgtatga cattttgaag tgaattaaat atttttgaac atgcttcttc gacagccagt
     2641 gttatatttt tcagatcaac acaaagcaca atgattactc gaaattcagt attttcaaat
     2701 ttacatattt aaagtcatgc aagctgtaac ttccctgtca aaattactgg ctgccaaatt
     2761 tatacctgtt tcttcagctg taccttttga tatttaaagt ttttaaattt ctgtaaagta
     2821 gattttgtag aatgtaatgt gttcactgcc tttgtgaagc ggtatataat tgtataattt
     2881 ctgtgtgtaa actgaatgct tgggctttca atacagtatt catataaagc aataaatatt
     2941 aatgttatga aatatttgac tacattttta tcaaaatatg aaagaatccc ccctttttta
     3001 gtttcagata cctgaactac acagatgagc ttctaaaact gatgcaaaca gtttctgaca
     3061 ctgtataata tgcttttggg tgatttgggg ggcaaccaca agttttgcgt tttgactact
     3121 taaatcatca tggctataaa taccaaaacg atttggatcc atttatgttt gtaggataat
     3181 atactactga ctgacttgac tgtcaggttc acaacagcta gatgatatat ttatgactat
     3241 gtctaatagt tgaaataaaa tctgaatatt gatttactat acccaagagg ggagaaaaat
     3301 taaccattgt aaatttttaa aaaatttttc aaaaatgtta aaatgaggca aatttaagtt
     3361 tacaaatttt gaaattttct tttgaatatt tatgaaattg tcagtaaact tacctaagat
     3421 cctgtgacct tttgatattt tttattttaa ttgtagtgcc atggaccatt tgtaaacaaa
     3481 ttgatttact tttgttggtt gtaagttgaa gatttagcat tatgactttg aggtctgtgg
     3541 ttttatttgt aaacttgcaa ttgctatatt tgcaagggca aatgtatttc tttattaaat
     3601 aaagtacaat aatggtgaat gtaccaaaat gacatcactt aactctatga gagatctgca
     3661 ttttaatcta tagtttaata gttttaatat ttattagata ttcatatgtt gatcatagat
     3721 caaacttgtt gctgtttata cagataattg tagaatgctc atggaatatc tttagggtag
     3781 gtggaatact tctgtagtta aattgggaaa ccttgttcag ctggttttag atattgatgg
     3841 ccatttggaa gtaaatttcc gcaggtattc ataggtgcac ttaacacaga ctttgcttaa
     3901 tgaaaatgtc agttctaata gtaactgatt cacttctgaa cagaagtgat tttaggcata
     3961 tttcttaaca tatatcaagc aaagtcctgt taaaagatct aaatgaagaa tggagacctc
     4021 agtgattaaa gatattttgt ttctgacctt gagcagattg cttacctgtt ctctagacta
     4081 taacccaaca tgtaaaaaaa atttgaagat ggtgatgagg aaagtgagat atatatatat
     4141 atatgtatta tgtttctagc acttttccct tttaaaaagt gaaaatatcc ttgtacattt
     4201 ttgaaaaata tattttcagt tctgaaaaat gtagcagaag tagtgaaaat gtcatatttt
     4261 aaatgttgat tattagataa atttaacctg cttagggttt attgtaacta cacctttcag
     4321 acgtgtgttt tggagtagtg gaattgccag ccaggccctg tggcttggaa aggcatccca
     4381 gaaatcctcg gccagaaggt gtggcttgtt aaagcattga gattcagagt attttgtttt
     4441 gccggtgtag ataggcatgt atttatgcat ttttgcattt gtaaaatcaa cttttcaaat
     4501 aatgtaaatg taatatacta gtttacttaa aggtacttgg gcagaatcta aagctgctac
     4561 aatgtttgat tatgaaaaaa atgtaacatg gtaaggatga aaatgcaact tacaaaacca
     4621 aaggaattaa aaattttcgg tagtgtttca aattgtcttc tgaacaggaa tttaacattg
     4681 gttttgatga aggtgagggt cagttctcaa gatttgtgct aatcataaaa tgaatgaatg
     4741 caaaacacct tgtaatttca tatggaatta taaaaattag gtttgctggg ttttggccta
     4801 ataagagtgc tagtatgtat tggttagact acatcttact atttcacatt ttaaaaatca
     4861 gtacactctt caggattttc ttttatttca acttggagcc tagattactt tgccaaatgt
     4921 attattttca taatgcaata aaatataaat tagaata
//
