LOCUS       NM_001042388            3923 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 4 regulatory subunit 1 (PPP4R1),
            transcript variant 1, mRNA.
ACCESSION   NM_001042388
VERSION     NM_001042388.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Xu,W., Hua,Z., Wang,Y., Tang,W., Ou,W., Liu,F., Yang,Y., Ding,W.,
            Wang,Z., Cui,L., Ge,W., Gu,Y., Wang,X., Chen,Y., Liu,C.Y. and Du,P.
  TITLE     AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c
            mediated IKK dephosphorylation in an autophagy-independent manner
  JOURNAL   Cell Death Differ 31 (5), 618-634 (2024)
   PUBMED   38424148
  REMARK    GeneRIF: AMBRA1 promotes intestinal inflammation by antagonizing
            PP4R1/PP4c mediated IKK dephosphorylation in an
            autophagy-independent manner.
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   He,Z., Zhong,Y., Lv,T., Wang,J., Jin,Y., Li,F. and Hu,H.
  TITLE     PP4R1 promotes glycolysis and gallbladder cancer progression
            through facilitating ERK1/2 mediated PKM2 nuclear translocation
  JOURNAL   Cancer Lett 586, 216677 (2024)
   PUBMED   38301910
  REMARK    GeneRIF: PP4R1 promotes glycolysis and gallbladder cancer
            progression through facilitating ERK1/2 mediated PKM2 nuclear
            translocation.
REFERENCE   3  (bases 1 to 3923)
  AUTHORS   Zhu,X., Chen,X., Shen,X., Liu,Y., Fu,W., Wang,B., Zhao,L., Yang,F.,
            Mo,N., Zhong,G., Jiang,S. and Yang,Z.
  TITLE     PP4R1 accelerates the malignant progression of NSCLC via
            up-regulating HSPA6 expression and HSPA6-mediated ER stress
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (1), 119588 (2024)
   PUBMED   37739270
  REMARK    GeneRIF: PP4R1 accelerates the malignant progression of NSCLC via
            up-regulating HSPA6 expression and HSPA6-mediated ER stress.
REFERENCE   4  (bases 1 to 3923)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3923)
  AUTHORS   Wang,B., Pan,L.Y., Kang,N. and Shen,X.Y.
  TITLE     PP4R1 interacts with HMGA2 to promote non-small-cell lung cancer
            migration and metastasis via activating MAPK/ERK-induced
            epithelial-mesenchymal transition
  JOURNAL   Mol Carcinog 59 (5), 467-477 (2020)
   PUBMED   32077156
  REMARK    GeneRIF: PP4R1 interacts with HMGA2 to promote non-small-cell lung
            cancer migration and metastasis via activating MAPK/ERK-induced
            epithelial-mesenchymal transition.
REFERENCE   6  (bases 1 to 3923)
  AUTHORS   Toyo-oka,K., Mori,D., Yano,Y., Shiota,M., Iwao,H., Goto,H.,
            Inagaki,M., Hiraiwa,N., Muramatsu,M., Wynshaw-Boris,A., Yoshiki,A.
            and Hirotsune,S.
  TITLE     Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and
            microtubule organization via NDEL1 dephosphorylation
  JOURNAL   J Cell Biol 180 (6), 1133-1147 (2008)
   PUBMED   18347064
REFERENCE   7  (bases 1 to 3923)
  AUTHORS   Gingras,A.C., Caballero,M., Zarske,M., Sanchez,A., Hazbun,T.R.,
            Fields,S., Sonenberg,N., Hafen,E., Raught,B. and Aebersold,R.
  TITLE     A novel, evolutionarily conserved protein phosphatase complex
            involved in cisplatin sensitivity
  JOURNAL   Mol Cell Proteomics 4 (11), 1725-1740 (2005)
   PUBMED   16085932
REFERENCE   8  (bases 1 to 3923)
  AUTHORS   Zhang,X., Ozawa,Y., Lee,H., Wen,Y.D., Tan,T.H., Wadzinski,B.E. and
            Seto,E.
  TITLE     Histone deacetylase 3 (HDAC3) activity is regulated by interaction
            with protein serine/threonine phosphatase 4
  JOURNAL   Genes Dev 19 (7), 827-839 (2005)
   PUBMED   15805470
REFERENCE   9  (bases 1 to 3923)
  AUTHORS   Wada,T., Miyata,T., Inagi,R., Nangaku,M., Wagatsuma,M., Suzuki,D.,
            Wadzinski,B.E., Okubo,K. and Kurokawa,K.
  TITLE     Cloning and characterization of a novel subunit of protein
            serine/threonine phosphatase 4 from mesangial cells
  JOURNAL   J Am Soc Nephrol 12 (12), 2601-2608 (2001)
   PUBMED   11729228
REFERENCE   10 (bases 1 to 3923)
  AUTHORS   Kloeker,S. and Wadzinski,B.E.
  TITLE     Purification and identification of a novel subunit of protein
            serine/threonine phosphatase 4
  JOURNAL   J Biol Chem 274 (9), 5339-5347 (1999)
   PUBMED   10026142
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR006046.1, AK027064.1,
            BC060829.1 and AF100744.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001042388.2.
            
            Summary: This gene encodes one of several alternate regulatory
            subunits of serine/threonine protein phosphatase 4 (PP4). The
            protein features multiple HEAT repeats. This protein forms a
            complex with PP4RC. This complex may have a distinct role from
            other PP4 complexes, including regulation of HDAC3 (Zhang et al.,
            PMID: 15805470). There is also a transcribed pseudogene on
            chromosome 20. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jun 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer protein (isoform a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK027064.1, AF200478.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000400556.8/ ENSP00000383402.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DR006046.1         56-56
            2-2833              AK027064.1         1-2832
            2834-3804           BC060829.1         2783-3753
            3805-3923           AF100744.1         2020-2138
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.22"
     gene            1..3923
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="protein phosphatase 4 regulatory subunit 1"
                     /db_xref="GeneID:9989"
                     /db_xref="HGNC:HGNC:9320"
                     /db_xref="MIM:604908"
     exon            1..82
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2928
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="isoform a is encoded by transcript variant 1;
                     serine/threonine phosphatase 4; serine/threonine-protein
                     phosphatase 4 regulatory subunit 1"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform a"
                     /protein_id="NP_001035847.1"
                     /db_xref="CCDS:CCDS42412.1"
                     /db_xref="GeneID:9989"
                     /db_xref="HGNC:HGNC:9320"
                     /db_xref="MIM:604908"
                     /translation="MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEM
                     LTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAVLERISRLADDSEPTV
                     RAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLALLE
                     QELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFC
                     EMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECF
                     MAVSCATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKE
                     ESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASG
                     KPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQ
                     ELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELE
                     EMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNC
                     KINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQ
                     YLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRT
                     LAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREY
                     LYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIALNLCADKVSSVR
                     WISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDC
                     LPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTI
                     MALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY"
     exon            83..127
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            128..263
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            264..370
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            371..513
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..660
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            661..768
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..834
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            835..993
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1121
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1648
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1821
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1917
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2103
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2265
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2366
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2487
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2622
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2764
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..3923
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3781..3786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="hexamer: AATAAA"
     polyA_site      3800
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="major polyA site"
     regulatory      3850..3855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="hexamer: AATAAA"
     polyA_site      3873
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
     regulatory      3900..3905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
                     /note="hexamer: ATTAAA"
     polyA_site      3923
                     /gene="PPP4R1"
                     /gene_synonym="MEG1; PP4(Rmeg); PP4R1"
ORIGIN      
        1 accggctccc cggctcgcgc cgctccatgt agccccggct ccccgggcgg ccgcggagga
       61 gggggcgacc acaagatggc ggacctctcg ctgcttcagg aggacctgca ggaggacgca
      121 gacggatttg gtgtggatga ctacagctca gagtctgatg tgattattat accttcagcc
      181 ctggactttg tctcacaaga tgaaatgttg acgcccctgg ggagattgga caagtatgct
      241 gcaagtgaga acatatttaa cagacaaatg gtggcccgga gtttgctcga taccttgagg
      301 gaagtctgcg atgatgaaag agattgtatt gctgttttgg aaagaattag cagattggcc
      361 gatgattcag aaccaactgt gagagcggag ctgatggaac aggtgcctca catcgcactg
      421 ttttgtcaag aaaaccggcc ttcaatacca tatgcttttt caaaattctt actacctatt
      481 gtggttagat accttgcaga tcagaataat caggtgagga aaacaagtca ggcagctttg
      541 ctggctctgt tggagcagga gctcattgaa cgatttgatg tggagaccaa agtgtgccct
      601 gtcctcatag agctgacagc cccagatagc aatgatgatg tgaaaacaga agctgtggct
      661 ataatgtgca aaatggctcc catggttggg aaggatatta cagagcgtct tatcctccct
      721 aggttttgtg agatgtgctg cgattgcaga atgtttcacg ttcgaaaggt ctgtgctgcc
      781 aattttggag atatttgcag tgtagttggc cagcaagcta ctgaagaaat gttgctgccc
      841 agatttttcc agctttgttc tgataatgta tggggagtcc gaaaggcttg tgctgaatgc
      901 ttcatggcgg tttcatgtgc aacatgtcaa gaaatccgac ggaccaaatt atcagcactt
      961 tttattaatt tgatcagtga tccttcacgt tgggttcgcc aagcagcttt tcagtctctg
     1021 ggacctttca tatctacttt tgctaatcca tctagctcag gccagtattt taaagaagaa
     1081 agcaaaagtt cagaagagat gtcagtagaa aacaaaaata ggaccagaga tcaagaagcc
     1141 ccagaggatg tacaagtcag gccagaggat actccttcag atctcagtgt tagtaattcc
     1201 agtgtcatac tggaaaacac gatggaagac catgctgctg aggcatccgg gaagcctcta
     1261 ggtgaaatta gtgttccact ggacagctct ttactttgta ctttgtcctc agaatctcac
     1321 caggaagcag ctagtaatga gaatgataaa aaacctggta actacaaatc tatgttacga
     1381 ccagaggttg gcaccacttc acaagattca gctctcttag atcaggaatt gtataactcc
     1441 ttccatttct ggaggactcc tcttcctgaa atagatctag acatagagct tgaacagaac
     1501 tctgggggaa aacccagccc agagggacca gaggaagaat ctgagggccc tgtgcccagt
     1561 tctccaaaca tcaccatggc caccagaaag gaactggaag aaatgataga aaatctagag
     1621 ccccacattg atgatccaga tgttaaagca caagtggaag tgctgtccgc tgcactacgt
     1681 gcttccagcc tggatgcaca tgaagagacc atcagtatag aaaagagaag tgatttgcaa
     1741 gatgaactgg atataaatga gctaccaaat tgtaaaataa atcaagaaga ttctgtgcct
     1801 ttaatcagcg atgctgttga gaatatggac tccactcttc actatattca cagcgattca
     1861 gacttgagca acaatagcag ttttagccct gatgaggaaa ggagaactaa agtacaagat
     1921 gttgtacctc aggcgttgtt agatcagtat ttatctatga ctgacccttc tcgtgcacag
     1981 acggttgaca ctgaaattgc taagcactgt gcatatagcc tccctggtgt ggccttgaca
     2041 ctcggaagac agaattggca ctgcctgaga gagacgtatg agactctggc ctcagacatg
     2101 cagtggaaag ttcgacgaac tctagcattc tccatccacg agcttgcagt tattcttgga
     2161 gatcaattga cagctgcaga tctggttcca atttttaatg gatttttaaa agacctcgat
     2221 gaagtcagga taggtgttct taaacacttg catgattttc tgaagcttct tcatattgac
     2281 aaaagaagag aatatcttta tcaacttcag gagtttttgg tgacagataa tagtagaaat
     2341 tggcggtttc gagctgaact ggctgaacag ctgattttac ttctagagtt atatagtccc
     2401 agagatgttt atgactattt acgtcccatt gctctgaatc tgtgtgcaga caaagtttct
     2461 tctgttcgtt ggatttccta caagttggtc agcgagatgg tgaagaagct gcacgcggca
     2521 acaccaccaa cgttcggagt ggacctcatc aatgagcttg tggagaactt tggcagatgt
     2581 cccaagtggt ctggtcggca agcctttgtc tttgtctgcc agactgtcat tgaggatgac
     2641 tgccttccca tggaccagtt tgctgtgcat ctcatgccgc atctgctaac cttagcaaat
     2701 gacagggttc ctaacgtgcg agtgctgctt gcaaagacat taagacaaac tctactagaa
     2761 aaagactatt tcttggcctc tgccagctgc caccaggagg ctgtggagca gaccatcatg
     2821 gctcttcaga tggaccgtga cagcgatgtc aagtattttg caagcatcca ccctgccagt
     2881 accaaaatct ccgaagatgc catgagcaca gcgtcctcaa cctactagaa ggcttgaatc
     2941 tcggtgtctt tcctgcttcc atgagagccg aggttcagtg ggcattcgcc acgcatgtga
     3001 cctgggatag ctttcggggg aggagagacc ttcctctcct gcggacttca ttgcaggtgc
     3061 aagttgccta cacccaatac cagggatttc aagagtcaag agaaagtaca gtaaacacta
     3121 ttatcttatc ttgactttaa ggggaaataa tttctcagag gattataatt gtcaccgaag
     3181 ccttaaatcc ttctgtcttc ctgactgaat gaaacttgaa ttggcagagc attttcctta
     3241 tggaagggat gagattccca gagacctgca ttgctttctc ctggttttat ttaacaatcg
     3301 acaaatgaaa ttcttacagc ctgaaggcag acgtgtgccc agatgtgaaa gagaccttca
     3361 gtatcagccc taactcttct ctcccaggaa ggacttgctg ggctctgtgg ccagctgtcc
     3421 agcccagccc tgtgtgtgaa tcgtttgtga cgtgtgcaaa tgggaaagga ggggttttta
     3481 catctcctaa aggacctgat gccaacacaa gtaggattga cttaaactct taagcgcagc
     3541 atattgctgt acacatttac agaatggttg ctgagtgtct gtgtctgatt ttttcatgct
     3601 ggtcatgacc tgaaggaaat ttattagacg tataatgtat gtctggtgtt tttaacttga
     3661 tcatgatcag ctctgaggtg caacttcttc acatactgta catacctgtg accactcttg
     3721 ggagtgctgc agtctttaat catgctgttt aaactgttgt ggcacaagtt ctcttgtcca
     3781 aataaaattt attaataaga tctatagaga gagatatata cacttttgat tgttttctag
     3841 atgtctacca ataaatgcaa tttgtgacct gtattaatga tttaaagtgg ggaaactaga
     3901 ttaaaatatt tgtcttttaa cta
//
