LOCUS       NM_000945               3024 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 3 regulatory subunit B, alpha
            (PPP3R1), mRNA.
ACCESSION   NM_000945
VERSION     NM_000945.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Li,M., Gong,W., Chen,J., Zhang,Y., Ma,Y. and Tu,X.
  TITLE     PPP3R1 Promotes MSCs Senescence by Inducing Plasma Membrane
            Depolarization and Increasing Ca2+ Influx
  JOURNAL   Int J Mol Sci 24 (5), 4421 (2023)
   PUBMED   36901851
  REMARK    GeneRIF: PPP3R1 Promotes MSCs Senescence by Inducing Plasma
            Membrane Depolarization and Increasing Ca[2+] Influx.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3024)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3024)
  AUTHORS   Miyata,H., Oura,S., Morohoshi,A., Shimada,K., Mashiko,D., Oyama,Y.,
            Kaneda,Y., Matsumura,T., Abbasi,F. and Ikawa,M.
  TITLE     SPATA33 localizes calcineurin to the mitochondria and regulates
            sperm motility in mice
  JOURNAL   Proc Natl Acad Sci U S A 118 (35) (2021)
   PUBMED   34446558
REFERENCE   5  (bases 1 to 3024)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3024)
  AUTHORS   Loh,C., Shaw,K.T., Carew,J., Viola,J.P., Luo,C., Perrino,B.A. and
            Rao,A.
  TITLE     Calcineurin binds the transcription factor NFAT1 and reversibly
            regulates its activity
  JOURNAL   J Biol Chem 271 (18), 10884-10891 (1996)
   PUBMED   8631904
REFERENCE   7  (bases 1 to 3024)
  AUTHORS   Kissinger,C.R., Parge,H.E., Knighton,D.R., Lewis,C.T.,
            Pelletier,L.A., Tempczyk,A., Kalish,V.J., Tucker,K.D.,
            Showalter,R.E., Moomaw,E.W. et al.
  TITLE     Crystal structures of human calcineurin and the human
            FKBP12-FK506-calcineurin complex
  JOURNAL   Nature 378 (6557), 641-644 (1995)
   PUBMED   8524402
REFERENCE   8  (bases 1 to 3024)
  AUTHORS   Kawamura,A. and Su,M.S.
  TITLE     Interaction of FKBP12-FK506 with calcineurin A at the B
            subunit-binding domain
  JOURNAL   J Biol Chem 270 (26), 15463-15466 (1995)
   PUBMED   7541044
REFERENCE   9  (bases 1 to 3024)
  AUTHORS   Guerini,D., Krinks,M.H., Sikela,J.M., Hahn,W.E. and Klee,C.B.
  TITLE     Isolation and sequence of a cDNA clone for human calcineurin B, the
            Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein
            phosphatase
  JOURNAL   DNA 8 (9), 675-682 (1989)
   PUBMED   2558868
REFERENCE   10 (bases 1 to 3024)
  AUTHORS   King,M.M., Huang,C.Y., Chock,P.B., Nairn,A.C., Hemmings,H.C. Jr.,
            Chan,K.F. and Greengard,P.
  TITLE     Mammalian brain phosphoproteins as substrates for calcineurin
  JOURNAL   J Biol Chem 259 (13), 8080-8083 (1984)
   PUBMED   6330098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC017083.7, BM468705.1, BC027913.1 and BQ228635.1.
            
            On Nov 22, 2018 this sequence version replaced NM_000945.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027913.1, SRR18074967.2554985.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000234310.8/ ENSP00000234310.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC017083.7         172031-172043       c
            14-381              BM468705.1         31-398
            382-2722            BC027913.1         365-2705
            2723-2733           BQ228635.1         732-742
            2734-3024           BC027913.1         2716-3006
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p14"
     gene            1..3024
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /note="protein phosphatase 3 regulatory subunit B, alpha"
                     /db_xref="GeneID:5534"
                     /db_xref="HGNC:HGNC:9317"
                     /db_xref="MIM:601302"
     exon            1..408
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             406..918
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /EC_number="3.1.3.16"
                     /note="protein phosphatase 2B regulatory subunit B alpha;
                     calcineurin B, type I (19kDa); protein phosphatase 2B
                     regulatory subunit 1"
                     /codon_start=1
                     /product="calcineurin subunit B type 1"
                     /protein_id="NP_000936.1"
                     /db_xref="CCDS:CCDS46310.1"
                     /db_xref="GeneID:5534"
                     /db_xref="HGNC:HGNC:9317"
                     /db_xref="MIM:601302"
                     /translation="MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFM
                     SLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMD
                     KDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGL
                     DIHKKMVVDV"
     exon            409..448
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     exon            449..625
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     exon            626..685
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     exon            686..870
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     exon            871..3024
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3005..3010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /note="hexamer: AATAAA"
     polyA_site      3024
                     /gene="PPP3R1"
                     /gene_synonym="CALNB1; CNB; CNB1"
                     /note="major polyA site"
ORIGIN      
        1 gtgccttgag gttgcgggtc agcgcgagcc gctgcagtga gtccgtcacg gctccggcgc
       61 gagcgcgagg ctgcagcccc cgagtttccc ggccgtcttc gccccctctc cccctccttt
      121 cttcttctct gcctctcctg cctctcgccg ctgctcctcc cgcgctctcc ggctctgaat
      181 ctcgacctta atttatttcc ccctaccctg cccgctccct cgcgtgccca atcgcccggc
      241 cggcgcgggc cccgcggcgc cgcctcccct cccccacgcg cgccccctcc ccgccggcga
      301 cccgagggcc gcagctgggc cgccgccgcc gtttcctgcg agccagcctg agcgcaacac
      361 ttctccgagc cagcgagcca gcgagccgcc gacccgccga gcaaaatggg aaatgaggca
      421 agttatcctt tggaaatgtg ctcacacttt gatgcggatg aaattaaaag gctaggaaag
      481 agatttaaga agcttgattt ggacaattct ggttctttga gtgtggaaga gttcatgtct
      541 ctgcctgagt tacaacagaa tcctttagta cagcgagtaa tagatatatt cgacacagat
      601 gggaatggag aagtagactt taaagaattc attgagggcg tctctcagtt cagtgtcaaa
      661 ggagataagg agcagaaatt gaggtttgct ttccgtatct atgacatgga taaagatggc
      721 tatatttcca atggggaact cttccaggta ttgaagatga tggtggggaa caatctgaaa
      781 gatacacagt tacagcaaat tgtagacaaa accataataa atgcagataa ggatggagat
      841 ggaagaatat cctttgaaga attctgtgct gttgtaggtg gcctagatat ccacaaaaag
      901 atggtggtag atgtgtgact cttatcagag agtaccaccc aacacttttg ctttcttctc
      961 catctctgaa gatctgctca agacgtccag caatgctctc tgtgtattta aatggaagta
     1021 tttttctctg tgaagccaca ttttccaaca tgagcctcat gaagccaact aagtgttatt
     1081 gaactgtaat tctctcaata actcagtgta gcactttaaa gtctgaagga cagcaacatg
     1141 aaaagagcat atcaatgtgg tggagaaagg gaaggggttg gctttttaat ttatttttct
     1201 tcatctttta taacaagaaa gtatctatat atacatatgt aaatatttat atatagatat
     1261 atgtagcttt ctatatatgt agtaggttgg ctttaattta atatacttga ttcagaaaca
     1321 aaacaataga gtacaaaagt gccaagcaga acataaaaca tccttacttt tatttcacac
     1381 agttttatat atagatagaa gactgtacaa tttgagccgg gtgttaggcc agctattttc
     1441 cttttcctgt gctttctcct ttgagagatt gacaaagcat ttgttaacgt cctattattt
     1501 accttaatta catttttgta acaaaggagt ctgtaacttt atttatactt atgaatatat
     1561 ccagggacta cttctcattg ctgagcagct tttaatacac ctctgcttga ggagaaagtc
     1621 tagttcattg ctactgccaa gagctagttc ttgtgttcat atagtaactg cacagggctt
     1681 atagctgctt cattctgcta ctttgtaact aggagccatt gcatttatta aatgtccctc
     1741 agtaacgtta agtgctagtt gtgattttat acataaaggc cagaagctgt ctgaggcaat
     1801 catgattgat tgtatgtatc acttactgaa gaatacctga agtgatcatg taactactta
     1861 taagggatat ccatttgttt gattacatgg gtaaataatt tgtcattaaa cttgtgtttg
     1921 aatcatgaat tcccttgttt caaaagactt gcagctaatc taaaaaactg gtgatattta
     1981 atatgcatgt atgtatctaa acacccacat atatttgtgg tttaagtgtg agaaatcttg
     2041 ctaatctata tgccacagaa gagcaaaatt gtatccaaat ttatgccact taaatttctt
     2101 taccacgagg gatagagcat gcatactggt ttttttttct tgatttgccc atataattgg
     2161 taatggataa cttaataaat ttgtgtgata taaaagtagt gtatcatgtt ctactgtttg
     2221 atcttatccc ttcccatttc tgcaaaggta ctgttagctg gaagaaaaac attttggtta
     2281 gctttgtatg acagtagtct ttccctttat agtttctcta taaaaactgg ttttaaaatc
     2341 agtggaaaag ggcaggttga atcaaggtga atcaatctga aattgagcac acctgcctgc
     2401 catcgctgtt ccttcaactg agtgctgcac atcatgggct ctgtctgtga gagaaaaatc
     2461 ccggtgcttg gtgtccttgc atgacatgga gttttgcatg tagatcaatt taaaatgtac
     2521 ctcttgttta cataatttgc ataattttaa aagataatgt tgccaaactt tggaaatgtt
     2581 aatgttcaga ctgaaaatct ccactacatg taactttctt cctctggatc agtggcatgg
     2641 cttataatcc cagccagtgg tttgaactgt tccagtgtca actgccatgt gctctgcttc
     2701 aagggggaac tagccttttg tgaatttttt gtacataagt atttgttaca aatattttag
     2761 caaatgcttt ctatttctct tgcttgtgca tatcttggct ggcgttacag aaaaatagtg
     2821 taaacattat ttccttaccg gggaatgagg gttttttctt tttctttttt tttttttttt
     2881 tttttagttt gtgtgtgggg gtgggtaagg gaggggatgg tttatgttga atgtttagtt
     2941 tttcttctgc atgatacgtc atgttgtggg atctttagaa aacttcatac tgtatgaata
     3001 agaaaataaa atatttgaaa cttg
//
